Storage Time Research Articles

Physico-mechanical parameters of rock materials used in open pit reclamation – a case study of CTL Maczki-Bór S.A. mine in Poland

The aim of the research was to assess the variability of selected physical and mechanical properties of Carboniferous barren rocks in the Upper Silesian Coal Basin and to analyze the impact of time and increasing vertical pressure on the mechanical parameters of the mixture of these rocks used in the reclamation of the open pit. Both type of tests were conducted in a strength press. For lithologically and stratigraphically differentiated rock samples, strength parameters in various states of humidity (air-dried and capillary saturation state) were determined, including compressive strength, cohesion and the angle of internal friction. It was found that the strength of all types of rock increases with geological age, however saturation of these rocks with water causes their structural weakening, which is especially noticeable in the case of sandstone and claystone. In the case of mudstone, due to their structure, the reduction of strength after saturation with water is not so significantly noticeable. In the second part of the laboratory tests, 5 samples of Carboniferous post-mining waste with different storage times in the open pit were subjected to the assessment of changes in density and compressibility in relation to increasing values of vertical pressure. It was found that the long-term depositing of waste in the excavation is conducive to the intensive development of hypergenic processes. This contributes to the disintegration of sedimentary rocks, which causes changes in their physical and mechanical parameters. This phenomenon is additionally intensified by the increasing vertical pressure with thickness of waste layers.

Evaluation of trypsin-like immunoreactivity, pancreatic lipase immunoreactivity, cobalamin, and folate in zoo-housed African painted dogs (Lycaon pictus) at a North American zoological facility.

Assess markers for pancreatic function and gastrointestinal malabsorption in African painted dogs (Lycaon pictus), including canine trypsin-like immunoreactivity (cTLI), canine pancreatic lipase immunoreactivity (cPLI), cobalamin, and folate at one North American facility. 15 healthy African painted dogs held at one institution were sampled during routine health examinations. Blood was collected at routine health examinations, and serum was separated and stored until testing. Serum was analyzed for cTLI, cPLI, cobalamin, and folate. The results were evaluated for correlation to sex, age, and storage time of samples. All individuals had cTLI and folate levels below normal reference ranges for domestic dogs (< 5.0 µg/L and < 7.7 µg/L, respectively). Cobalamin values were within or above reported domestic dog ranges, and cPLI values were within range as well. No analytes were significantly influenced by sex or time in storage, while cTLI was positively correlated with age. cTLI and folate did not fall within normal domestic canid reference ranges in this population of healthy African painted dogs. Clinical interpretation of these values based on domestic canid recommendations would indicate clinical disease, which was not apparent in this population. Analytes for pancreatic function and malabsorption or gastrointestinal indicators, including cTLI, cPLI, and folate, in African painted dogs should be interpreted with caution when using domestic dog references ranges.

Key Aroma Differences in Volatile Compounds of Aged Feng-Flavored Baijiu Determined Using Sensory Descriptive Analysis and GC×GC–TOFMS

Sensory descriptive analysis of aged feng-flavored Baijiu liquor indicated notable differences in samples of different ages. The samples of freshly distilled Baijiu and those with shorter storage times exhibit bran and fresh green flavors, whereas, with increasing storage time, honey, sweet, and floral fragrances are gradually enhanced. Samples of feng-flavored Baijiu were prepared using headspace solid-phase microextraction, followed by comprehensive two-dimensional gas chromatography time-of-flight mass spectrometry. A total of 496 compounds were identified in all samples, mainly categorized as 14 groups of substances, including esters and aldehydes. Interestingly, 42 of these substances were found in Feng-flavored Baijiu for the first time. Chemometrics was used to analyze the key differential compounds. First, 143 differential compounds closely related to aging were preliminarily screened, and principal component analysis revealed that these compounds were separated by baijiu age. Then, 65 differential compounds were selected by partial least squares discriminant analysis. Furthermore, 43 key differential compounds were selected by combined analysis with variable importance in projection and Pearson correlation coefficients. Partial least squares regression was used to study the correlation between the sensory properties and key differential compounds, and the results indicated that most compounds were closely related to the aging period of the Baijiu. The results of this study provide a theoretical basis and reference for flavor research on feng-flavored Baijiu.

The effect of synbiotic coating of flaxseed mucilage‐defatted rice bran carbohydrate on quality of dried mango, viability of Bifidobacterium animalis subsp. LactisBB12 on storage and simulating gastrointestinal condition

AbstractIn the present study, a synbiotic coating of flaxseed mucilage, defatted rice bran carbohydrate, and Bifidobacterium animalis subsp. Lactis BB12 was fabricated for coating dried mango slices (M‐P‐C). The control samples contained only probiotic bacteria without coating (M‐P). Several quality parameters (moisture, weight loss, shrinkage percentage, pH, firmness, and color) were assessed on specific storage circumstances (25°C, relative humidity (RH) = 22%.). In addition, the survival of Bifidobacterium animalis subsp. Lactis BB12 was evaluated on storage and under simulated gastrointestinal (GI) conditions. According to the results, the log number of Bifidobacterium animalis subsp. Lactis BB12 reached 8.1 and 6.2 for coated and uncoated samples, respectively, during the 45 days storage at 25°C (&gt;6 log CFU (log colony‐forming units)/g) and at finished stage of in vitro gastrointestinal circumstances, the log number of probiotic bacterial count reached 6.8 and 4 for coated and uncoated samples, respectively. The coating resulted in significantly less weight loss, moisture loss, and shrinkage of the mango slices than uncoated ones (p &lt; .05). The growth of yeasts and molds was undetectable in both samples. The results of acceptance experiments for M‐P and M‐P‐C dried mango samples showedthat there were no significant differences between M‐P and M‐P‐C samples (p &gt;.05), indeed in the case of purchase intention and overall acceptability. After reading the text highlighting, there was no significant difference in all attributes of M‐P‐C samples pre and post of reading text highlighting. It could be concluded that the synbiotic coating of mango slices improved the quality characteristics of the dried mango as well as viability of the probiotic bacteria at storage time and under simulated gastrointestinal conditions.

Characterizing the antioxidant and antifungal properties of nano‐encapsulated pistachio hull extract in fenugreek seed gum to maintain the quality and safety of fresh pistachio

AbstractThe quality of pistachio, one of the export products of Iran, will be decreased during storage as a result of mold spoilage, toxins production, and oil oxidation. This study aimed to investigate the capability of pistachio hull extract (PHE) loaded in fenugreek seed gum (FSG):whey protein isolate (WPI) nanoemulsion to control oil oxidation, and fungi growth in fresh pistachio nut during storage at 4°C. The total anthocyanin and total phenolic content of the PHE were 125.44 μg/g and 675.18 mg/g, respectively. The DPPH radical scavenging activity of PHE at 100 ppm was higher than that of tert‐butylhydroquinon (TBHQ). In comparison with other concentrations, 50 ppm showed the strongest antifungal activity against Aspergillus flavus, Aspergillus parasiticus, and Aspergillus nomius. All nanoemulsions have a mean size lower than 265 nm. The polydispersity index (PDI) of different nanoemulsions was lower than 0.3, and a negative zeta potential was observed. The encapsulation efficiency was higher than 67.0% and all nanoemulsions had spherical morphology. The pistachio nuts were coated with different coating solutions containing 0 and 100 ppm of PHE and stored at 4°C for 8 weeks. The results showed that the pistachio sample coated with a composite coating of WPI and FSG containing 100 ppm of PHE has a higher moisture content and lower changes in L*, a*, and b* indexes, oil oxidation, fungi development, and total mold and yeast count. This treatment exhibited higher overall acceptance than other samples at the end of storage time. The results of this study suggest the use of biodegradable coatings enriched with natural extracts that have high antioxidant and antifungal activities.

Proportions of mineral and organomineral fertilizers in onion cultivation

Organomineral fertilizers is an innovation in agriculture, since it uses organic matter enriched with minerals. The combination of mineral phosphate and organomineral fertilization can contribute to the development of a more sustainable and economical fertilization program for the crop. So, organomineral fertilization can be an alternative or complement to mineral fertilization. However, the careful selection of fertilizer sources and doses is essential to increase productivity in the onion crop and finding proportions between different fertilizers can be a promising choice for sustainable agriculture. The aim of the present research was to evaluate the effect of different proportions of mineral and organomineral fertilizers on the development, production and post-harvest quality of onion bulbs, cv. Sublime F1, under Cerrado conditions. The experiment was carried out in the field, in Cristalina, GO, Brazil, in a randomized block design, with four replications. Six treatments were used: five proportions of mineral fertilizer (M) with organomineral (OM) and the control (without additional fertilization). It was evaluated: length, width and number of leaves; productivity; soluble solids content; titratable acidity; and loss of fresh mass. Data were submitted to analysis of variance (ANOVA), at 1% (p≤0.01) and 5% (p≤0.05) of significance, and means compared by Tukey and Dunnett tests (p≤0.01 and p≤0.05). The fertilization proportions were submitted to regression (p≤0.05). The proportion of 50%M50%OM promotes higher bulb average mass and class 4 onion productivity. The proportions of fertilizers did not alter the postharvest characteristics (total soluble solids and total acidity) of the onion, which were affected only by the time of storage. Cultivar Sublime F1 showed good development for the region, conditions and fertilizers used.

Analysis of microcystins in alum water treatment sludges: holding times, temperatures, linearity of response, and sensitivity to pre-coagulation cell titers

ABSTRACT ELISA assays are a potential tool to screen for dissolved or cell bound microcystins in drinking water treatment sludges. In order to evaluate this potential more thoroughly, experiments were performed in alum sludges to: (1) evaluate the impacts of sample storage times, temperatures, and sludge composition on spiked microcystin-LR recovery by ELISA; (2) examine the linearity of ELISA responses to spiked microcystin-LR as a function of sludge composition; and (3) examine the sensitivity ELISA and LC/MS/MS to five different concentrations of microcystin-producing cyanobacteria entrained in sludges of two different compositions. During storage experiments, microcystin recovery efficiencies ranged from 85% to 125% across the range of 12 storage time and temperature combinations with recovery efficiencies in 7 of the 12 combinations falling into the 90% to 110% range. During the linearity experiments, linear models fit ELISA responses in all sludge compositions with R2 values ≥ 0.95. During the sensitivity studies, simple freeze/thaw/centrifugation processing combined with ELISA or LC/MS/MS analyses resulted in detection of microcystins in thickened sludges derived from pre-coagulation cell suspensions of 102–106 cells/mL. In addition, the relationships between toxin concentrations in sludges and the original cell titers were linear regardless of analytical method.

Metagenomic sequencing and reconstruction of 82 microbial genomes from barley seed communities

Barley (Hordeum vulgare) is essential to global food systems and the brewing industry. Its physiological traits and microbial communities determine malt quality. Although microbes influence barley from seed health to fermentation, there is a gap in metagenomic insights during seed storage. Crucially, elucidating the changes in microbial composition associated with barley seeds is imperative for understanding how these fluctuations can impact seed health and ultimately, influence both agricultural yield and quality of barley-derived products. Whole metagenomes were sequenced from eight barley seed samples obtained at different storage time points from harvest to nine months. After binning, 82 metagenome-assembled genomes (MAGs) belonging to 26 distinct bacterial genera were assembled, with a substantial proportion of potential novel species. Most of our MAG dataset (61%) showed over 90% genome completeness. This pioneering barley seed microbial genome retrieval provides insights into species diversity and structure, laying the groundwork for understanding barley seed microbiome interactions at the genome level.

Changes in water-soluble taste compounds of tilapia (Oreochromis niloticus) fillets subjected to different thawing methods during long-term frozen storage.

The taste of fish is highly dependent on the composition of free amino acids (FAAs) and nucleotides. The present study aimed to investigate the effect of long-term frozen storage periods (-18 °C, up to 6 months) and thawing methods [water thawing (WT, 25 °C), air thawing (AT, 25 °C), and chilled air thawing (CAT, 4 °C)] on the taste quality of tilapia (Oreochromis niloticus) fillets. The results showed that increase in bitter FAAs of CAT samples was 150.57% at 6 months of storage, which was lower than that of AT and WT. Glycine was the most abundant FAA and CAT maintained the highest sweet FAAs (249.90 mg/100 g). Additionally, the inosine monophosphate (IMP) of CAT samples were 1.18 and 1.09 times higher than that of WT and AT, respectively, at a frozen period of 6 months. In particular, the increase in equivalent umami concentration (EUC) values ranged from 24.25% to 103.16% in the three groups during the first 2 months. Data from principal component analysis (PCA) and orthogonal partial least-squares discrimination analysis (OPLS-DA) indicated that the taste quality was highly correlated with high levels of FAAs, hypoxanthine inosine (HxR) and hypoxanthine (Hx) as the storage time progressed. In general, CAT is beneficial in maintaining the taste quality of tilapia fillets during frozen storage, and frozen durations for 2 months enhances the umami flavor. This study provides useful information for the preservation of frozen aquatic products during the storage and thawing, and enrich the theoretical knowledge of the flavor chemistry of fish products. © 2024 Society of Chemical Industry.

Production and quality evaluation of a novel γ-aminobutyric acid-enriched yogurt

Objectiveγ-aminobutyric acid (GABA) is a neurotransmitter inhibitor that has beneficial effects on various health conditions such as hypertension, cognitive dysfunction, and anxiety. In this study, we investigated a novel yogurt naturally enriched with GABA using a Levilactobacillus brevis strain isolated in our laboratory; the specific optimum yogurt production conditions for this strain were determined.MethodsWe isolated an L. brevis strain and used it to produce yogurt naturally enriched with GABA. We explored the optimal conditions to enhance GABA yield, including fermentation temperature, inoculation amount, L-monosodium glutamate (L-MSG) concentration, fermentation time, and sucrose content. We also performed mixed fermentation with Streptococcus thermophilus and evaluated the quality of the yogurt.ResultsFollowing optimization (43°C, 8% inoculation amount, 1.5 g/L L-MSG, and 8% sucrose for 40 h of fermentation), the GABA yield of the yogurt increased by 2.2 times, reaching 75.3 mg/100 g. Mixed fermentation with S. thermophilus demonstrated favorable results, achieving a GABA yield akin to that found in some commercially available functional foods. Moreover, the viable microbe count in the GABA-enriched yogurt exceeded 1 × 108 cfu/mL, which is higher than that of commercial standards. The yogurt also exhibited a suitable water-holding capacity, viscosity, 3-week storage time, and favorable sensory test results.ConclusionThis study highlights the potential of naturally enriched GABA yogurt as a competitive commercial yogurt with beneficial health effects.

Preparation of modified atmosphere packaging based on the respiratory characteristics of cherry tomato and its freshness preservation application

Cherry tomato (Lycopersicon esculentum Mill.) belongs to the respiratory leap type of fruit, post-harvest respiratory metabolism exuberant, easy to lead to nutrient loss, tissue destruction, and then rotting and deterioration, seriously affecting its commercial value. Therefore, research on post-harvest preservation technology of cherry tomatoes is crucial for related industries. Spontaneous modified atmosphere film packaging technology can extend the storage period of fruits and vegetables by adjusting the gas concentration of the storage environment, so that the fruits and vegetables can be maintained at a level suitable for their storage. In this study, the respiration model of cherry tomatoes at different temperatures was established by measuring the respiration rate of cherry tomatoes at different temperatures. It was found that the respiration rate of cherry tomatoes increased with increasing temperature and gradually decreased with increasing storage time. T-test results showed that there was no significant difference between the experimental values and the predicted values. The chemical kinetic model and its equilibrium equation were used to determine the parameters of modified atmosphere packaging of cherry tomatoes. PEG/PVDF membranes and SPEEK/PEG/PVDF blend membranes were prepared with sulfonated polyether ether ketone (SPEEK), polyvinylidene fluoride (PVDF) and polyethylene glycol (PEG) according to the parameters of modified atmosphere membranes to keep cherry tomatoes fresh. The results showed that there was a significant effect of package method on the preservation parameters of cherry tomatoes, the SPEEK/PEG/PVDF membrane treatment group significantly reduced the weight loss rate of cherry tomatoes and maintained the lower film permeability and higher sound fruit rate, hardness, titratable acid (TA) content, VC content and total soluble solids (TSS) content of cherry tomatoes. It can effectively delay the ripening and senescence of cherry tomatoes and extend the fruit storage period.

Antioxidant Activity and Fatty Acid Profile of Sous-Vide Beef Marinated with Kiwiberry Fruit Pulp: Effects of Level Addition and Refrigerated Storage

The purpose of the study was to determine the antioxidant activity (AA) and fatty acid (FA) profile of sous-vide beef previously marinated in brine with a 10, 20 and 30% addition of kiwiberry (Actinidia arguta cv. ‘Ananasnaya’) fruit pulp, as well as changes in the parameters studied after 0, 1, 2 and 3 weeks of refrigerated storage in a vacuum package. The FA profile, FRAP (ferric-reducing antioxidant power assay), ABTS (2,2′-azinobis (3-ethylbenzthiazoline-6-acid)), total polyphenols, chlorophylls and carotenoids were also determined in the fruit pulp. Lipid indices for meat were calculated based on the obtained FA profile. The values of FRAP and ABTS of experimental meat products were significantly (p ≤ 0.05) higher than those of control samples but decreased with storage time. The proportion of unsaturated FA in the lipids of sous-vide meat was higher in samples with pulp than in control samples and insignificantly decreased with storage time. Meat marinated with kiwiberry pulp was characterized by a significantly (p ≤ 0.05) higher proportion of ALA (α-linolenic acid) and LA (linoleic acid), considerably affecting the more favorable value of polyunsaturated FA/saturated FA ratio. A troubling finding was the heightened level of palmitic acid (C16:0) in the lipids of beef subjected to 30% kiwiberry pulp, a factor recognized to play a significant role in the development of various diseases. Beef marinated with 20% kiwiberry pulp addition provides greater nutritional and health benefits than other sample variants because of optimal AA and FA profile changes during refrigerated storage.

Assessing the effect of sample storage time on viral detection using a rapid and cost-effective CTAB-based extraction method

BackgroundCassava leaf samples degrade quickly during storage and transportation from distant areas. Proper sampling and efficient, low-cost storage methods are critical for obtaining sufficient quality DNA and RNA for plant virus epidemiology and improving disease control understanding. This is useful when samples are collected from remote areas far from a laboratory or in developing countries where money and materials for virus diagnostics are scarce.ResultsThe effect of sample storage duration on nucleic acid (N.A.) quality on virus detection was investigated in this study. A simple, rapid, and cost-effective CTAB-based approach (M3) for single N.A. extraction was optimized and tested alongside two existing CTAB-based methods (M1 and M2) for N.A. extraction from fresh and herbarium cassava leaves stored for; 1, 8, 26, and 56 months. The amount and quality of DNA and RNA were determined using Nanodrop 2000 c U.V.–vis Spectrophotometer and agarose gel electrophoreses. The sample degradation rate was estimated using a simple mathematical model in Matlab computational software. The results show no significant difference in mean DNA concentration between M1 and M2 but a significant difference between M3 and the other two methods at p < 0.005. The mean DNA concentration extracted using M3 was higher for 1 and 8 months of leave storage. M3 and M2 produced high concentrations at 26 and 56 months of leave storage. Using a developed scale for quality score, M3 and M2 produced high-quality DNA from fresh samples. All methods produced poor-quality DNA and RNA at 8 and 26 months of leave storage and no visual bands at the age of 56 months. Statistically, there was a significant difference in the mean DNA quality between M1 and M2, but there was no significant difference between M3 and the other two methods at p < 0.005. However, Cassava brown streak virus (CBSV) and Ugandan cassava brown streak virus (UCBSV) were readily detected by RT-PCR from RNA isolated using M3. The quality of DNA declined per storage time at 0.0493 and 0.0521/month, while RNA was 0.0678 and 0.0744/month. Compared to the existing two methods, modified CTAB extracted enough high-quality N.A. in one-third the time of the existing two methods.ConclusionOur method provides cost-effective, quick, and simple processing of fresh and dry samples, which will quicken and guide the decision on when and what type of sample to process for plant disease management and surveillance actions.

Analyzing Hydrogen Flow Behavior Based on Deep Learning Sensor Selection Optimization Framework

Abstract With tremendous potential to lower the carbon footprint of power generation and as an alternative energy carrier for many applications, hydrogen has emerged as a key potential energy carrier. Understanding of geological conditions and the injection and production changes over time for hydrogen storage are paramount, requiring in situ reservoir sensing options. Determining the flow behavior is of critical importance in order to estimate hydrogen volumes within the reservoir. A novel AI-driven methodology for hydrogen flow behavior and volume estimation was demonstrated. For determining the S1 through S3 predicted hydrogen storage quantities, the framework is linked to an uncertainty estimate framework. The Pohokura field in New Zealand served as the basis for the framework's evaluation, and it performed acceptably in terms of identifying the hydrogen flow behaviors and quantities inside the subsurface reservoir. The framework is a significant first step in assessing the amount of hydrogen that can be stored in subterranean reservoirs for long-term hydrogen storage.

A new strategy to improve the quality of frozen chicken wings: High voltage electrostatic field combined with phosphorus-free water retaining agent

Freezing is a commonly used method for long-term storage of chicken wing products, of which disadvantages are mainly the product damage caused in the process. The aim of this study was to improve the freezing quality of chicken wings with a combination of phosphorus-free water retaining agent (WRA) and high-voltage electrostatic field (HVEF). The effect of WRA acting at different HVEF intensities (0, 1, 3, and 5 kV/cm) on the quality attributes of frozen chicken wings was investigated in 0, 7, 14, 21, 28 and 35 days of frozen storage. The results showed that WRA had functional properties of significantly improving the water holding capacity (WHC), color and texture properties, and fat stability of frozen chicken wing samples. The application of HVEF on this basis helped to promote the absorption of WRA and inhibit oxidative deterioration of chicken wing samples during frozen storage. Meanwhile, the combination of HVEF at 3 kV/cm was more prominent in terms of improvement in WHC, moisture content, color, protein secondary structure and microstructure integrity. This advantage had been consistently maintained with the extension of storage time. Overall, WRA combined with HVEF of 3 kV/cm can be used as an effective strategy to improve the freezing quality of chicken wing samples and has the potential to maintain the frozen chicken wing samples quality for a long time.

Mathematical Evaluation of Population Changes of Lactobacillus acidophilus and Bifidobacterium animalis ssp. lactis as Free and Encapsulated Cells in Butter

Growing butter markets, domestically and globally, provide opportunities for value-added variants of butter. Adding probiotics to butter could boosts its bioactivity; however, maintaining probiotic viability during storage is a major challenge. Mathematical analysis of probiotic population changes could help improve our understanding of how probiotics interact with butter and storage conditions. Two strains of probiotics in a 1:1 ratio as free cells or Whey Protein Hydrolysate–Maltodextrin (WPH-MD)-encapsulated cells, Lactobacillus acidophilus ATCC 4356 (LA5) and Bifidobacterium animalis ssp. lactis ATCC 27536 (BB12), were separately mixed into butter at 1% levels. Using analysis of covariance, a mathematical evaluation for probiotic population changes was performed by periodically determining viable counts, resulting in an adjusted R2 value of 0.98 and demonstrating a strong relationship between the dependent variable (log10 counts of probiotics) and independent variables (cell type, temperature of storage, and time of storage). After 21 days of storage, the number of free cells in butter dropped from 7.45 log10 CFU/g to 0.56 log10 CFU/g. On the other hand, it took 63 days for encapsulated cells to achieve 0.80 log10 CFU/g at the same temperature. The same trend persisted at −18 °C, indicating that the WPH-MD encapsulant had a protective effect.

Changes in amino acids, catechins and alkaloids during the storage of oolong tea and their relationship with antibacterial effect

The storage process has a significant impact on tea quality. Few is known about effect of storage on quality of oolong tea. This study aimed to assess the effect of different storage times on the key chemical components of oolong tea by measuring changes in catechin, free amino acid, and alkaloid content. Variation in the main substances was determined by principal component analysis and heat map analysis. The results revealed notable effects of the storage process on the levels of theanine, epigallocatechin gallate (EGCG), and glutamine. These findings suggest that these compounds could serve as indicators for monitoring changes in oolong tea quality during storage. Additionally, the study observed an increase in the antibacterial ability of tea over time. Correlation analysis indicated that the antibacterial ability against Micrococcus tetragenus and Escherichia coli was influenced by metabolites such as aspartic acid, threonine, serine, gamma-aminobutyric acid, ornithine, alanine, arginine, and EGCG. Overall, this study presents an approach for identifying key metabolites to monitor tea quality effectively with relatively limited data.

Physical and microbiological quality and oxidative stability in different storage periods of Angus-Nellore heifer meat in an integrated livestock-forest system

This study aimed to evaluate the physical and microbiological quality as well as the oxidative stability of meat stored frozen (30, 60, 90, 180, and 360 days) and obtained from Angus-Nelore heifers kept in a conventional system (CS; no shade available) and Integrated Livestock-Forest (ILF-1L and ILF-3L). Forty-eight ½ Nellore ½ Angus heifers with an average initial weight of approximately 276.70 ± 20.1 kg and an average age of nine months were distributed across a randomized block design with three treatments and four replicates. After slaughter, the Longissimus thoracis muscle was collected from each carcass for pH, lipid oxidation, meat color, and microbiological analyses. The physical and microbiological quality and oxidative stability of the meat were not affected by the tree component present in the pasture. Regarding the duration of frozen storage, it was found that the pH, meat color (a*, b*, C*, H*, O/M), and lipid oxidation increased as the period of storage increased (P&lt;0.05). The physical and microbiological quality, as well as the oxidative stability of the meat, was not affected by the tree component present in the pasture. However, the meat changed as the frozen storage time increased.

Effect of the 35 nm and 70 nm Size Exclusion Chromatography (SEC) Column and Plasma Storage Time on Separated Extracellular Vesicles

The technical difficulty of separating extracellular vesicles (EVs) from plasma proteins in human blood presents a significant hurdle in EV research, particularly during nano ultra-high-performance liquid chromatography–tandem mass spectrometric (UHPLC-MS/MS) analysis, where detecting “vesicular” proteins among abundant plasma proteins is challenging. Standardisation is a pressing issue in EV research, prompting collaborative global efforts to address it. While the MISEV guidelines offer valuable recommendations, unanswered questions remain, particularly regarding sample storage. We compared size exclusion chromatography (SEC) columns with pore sizes of 35 nm and 70 nm to identify fractions with minimal contaminating proteins and the highest concentration of small EVs (sEVs). Following column selection, we explored potential differences in the quality and quantity of sEVs isolated from platelet-free plasma (PFP) after long-term storage at −80 °C (&gt;2.5 years) compared to freshly drawn blood. Our methodologically rigorous study indicates that prolonged storage, under correct storage and processing conditions, does not compromise sEV quality. Both columns effectively isolated vesicles, with the 70 nm column exhibiting a higher abundance of “vesicular” proteins. We propose a relatively rapid and moderately efficient protocol for obtaining a comparatively pure sEV fraction from plasma, facilitating sEV processing in clinical trials.

Postharvest Treatment Effects on ‘Somerset Seedless’ Cold-Hardy Table Grapes

ABSTRACT Limited amount of information is available for cold-hardy table grape postharvest storage and strategies to extend the storage time. ‘Somerset Seedless’ is a cold-hardy table grape with a potential market for the Upper Midwest and Northern Great Plains. Postharvest treatments were assessed as a possible route to increase cold-hardy table grape shelf-life. In this study, a 1.4% chitosan postharvest treatment was tested on ‘Somerset Seedless’ grapes through 7 weeks of storage with temperature 1–4°C, humidity ≥90%. The effects were compared to two controls: one was diH2O, and the other one was acetic acid. The assessment included grape appearance traits (rachis, decay, mold, scattering, and splitting), physiochemical properties (TSS, pH and total acidity), antioxidant activity, as well as total phenolics and flavonoid content. In general, 1.4% chitosan, 1% acetic acid, and diH2O had a large impact on grape appearance during storage with limited impact on chemistry. Throughout 7 weeks of storage, no significant differences were observed in grape physiochemical and phytochemical changes. Chitosan performed the best for ‘Somerset Seedless’ postharvest storage in regard to the low rate of visible damage. Although acetic acid had similarly positive effects on mold control as chitosan treatment, it caused the highest split rate after 1 week and the highest shatter rate after 5 weeks of storage. Chitosan treated ‘Somerset Seedless’ still met the USDA standard of table grapes after 5 weeks. This study suggests chitosan postharvest treatments may have applications for enhancing the shelf-life of cold-hardy table grapes.