Time In Horses Research Articles

Detection of levamisole and its metabolites in horses after oral levamisole administration over seven days.

Levamisole is a regulated substance sometimes administered to racehorses to treat equine protozoal myelitis. Metabolites include compound II, aminorex, and pemoline. Aminorex and pemoline are Horseracing Integrity and Safety Authority-banned substances. Previous studies have examined single doses of the drug. This study examined the disposition of levamisole after 7 days of dosing. 6 healthy Thoroughbred geldings. Horses were treated with 500 mg (approx 0.91 to 1 mg/kg) of compounded levamisole hydrochloride paste PO every 12 hours for a total of 13 doses over 7 days. Serum and urine samples were analyzed for levamisole and its metabolites over a 28-day period. The terminal half-life of levamisole in serum was variable between horses. Following the last dose of levamisole on day 7, serum levamisole levels took 3 to 14 days (days 10 to 21) to fall below the limit of detection (LOD) in 5 of 6 horses. Serum from 1 horse remained over LOD on the last testing day (day 28). In urine, following the final dose (day 7), levamisole was below LOD on day 13 (6 days after final dose) and aminorex was below LOD on day 10 (3 days after final dose). Compound II was above LOD in 4 of 5 horses sampled on the last sampling day (day 28). Levamisole and its metabolites can be detected for variable lengths of time in horses, with detection lasting for days to weeks following multiple doses. This study supports the Racing Medication and Testing Consortium Advisory on levamisole, which suggests that clearance sample testing should be conducted on treated horses to verify elimination of levamisole and its metabolites.

Neonatal and maternal upregulation of antileukoproteinase in horses.

The end of gestation, ensuing parturition, and the neonatal period represent highly dynamic phases for immunological changes in both mother and offspring. The regulation of innate immune cells at the maternal-fetal interface during late term pregnancy, after birth, and during microbial colonization of the neonatal gut and other mucosal surfaces, is crucial for controlling inflammation and maintaining homeostasis. Innate immune cells and mucosal epithelial cells express antileukoproteinase (SLPI), which has anti-inflammatory and anti-protease activity that can regulate cellular activation. Here, we developed and validated new monoclonal antibodies (mAbs) to characterize SLPI for the first time in horses. Peripheral blood and mucosal samples were collected from healthy adults horses and a cohort of mares and their foals directly following parturition to assess this crucial stage. First, we defined the cell types producing SLPI in peripheral blood by flow cytometry, highlighting the neutrophils and a subset of the CD14+ monocytes as SLPI secreting immune cells. A fluorescent bead-based assay was developed with the new SLPI mAbs and used to establish baseline concentrations for secreted SLPI in serum and secretion samples from mucosal surfaces, including saliva, nasal secretion, colostrum, and milk. This demonstrated constitutive secretion of SLPI in a variety of equine tissues, including high colostrum concentrations. Using immunofluorescence, we identified production of SLPI in mucosal tissue. Finally, longitudinal sampling of clinically healthy mares and foals allowed monitoring of serum SLPI concentrations. In neonates and postpartum mares, SLPI peaked on the day of parturition, with mares returning to the adult normal within a week and foals maintaining significantly higher SLPI secretion until three months of age. This demonstrated a physiological systemic change in SLPI in both mares and their foals, particularly at the time around birth, likely contributing to the regulation of innate immune responses during this critical period.

Effect of Sugar Beet Pulp on the Composition and Predicted Function of Equine Fecal Microbiota.

The purpose of this study is to determine the effect of the partial replacement of dietary hay with sugar beet pulp (SBP) on the composition and predicted function of the fecal microbiota of healthy adult horses. Fecal samples were collected daily for 12 days from six adult horses after removal from pasture, including a five-day acclimation period, and a seven-day period following the introduction of SBP into their diet, and compared to six untreated horses over a comparable period. Fecal DNA was subjected to 16S rRNA amplicon sequencing and a longitudinal analysis was performed comparing the composition and predicted function. While no significant treatment-associated changes in the richness, alpha diversity, or beta diversity were detected, random forest regression identified several high-importance taxonomic features associated with change over time in horses receiving SBP. A similar analysis of the predicted functional pathways identified several high-importance pathways, including those involved in the production of L-methionine and butyrate. These data suggest that feeding SBP to healthy adult horses acutely increases the relative abundance of several Gram-positive taxa, including Cellulosilyticum sp., Moryella sp., and Weissella sp., and mitigates the predicted functional changes associated with removal from pasture. Large-scale studies are needed to assess the protective effect of SBP on the incidence of the gastrointestinal conditions of horses.

Thoroughbred Racehorses in Hong Kong Require Vitamin D Supplementation to Mitigate the Risk of Low Vitamin D Status.

There is a paucity of data relating to the vitamin D status of racehorses. We hypothesised that the management of racehorses in Hong Kong (HK) predisposes to low vitamin D status unless they receive dietary supplementation. Serum concentrations of 25-hydroxyvitamin D2 (25OHD2), 25-hydroxyvitamin D3 (25OHD3) and total 25-hydroxyvitamin D (total 25OHD) for 79 non-grazing HK racehorses were compared with those for 22 racehorses training in the United Kingdom (UK) that grazed for ≥1 h/d, and for which published data exists. A nested group of 41 HK horses was sampled twice to determine the effect of the duration in HK on vitamin D status. The HK horses had significantly lower serum concentrations of total 25OHD and 25OHD2 than the UK horses; 25OHD2 was undetectable in 15/79 HK sera and serum concentrations of 25OHD2 declined with the duration in HK. The main determinants of vitamin D status were assessed using linear regression; the retained variables were the 25OHD3 concentration and the duration in HK. The inverse relationship between the serum concentrations of 25OHD2 and 25OHD3, previously identified in humans, was observed for the first time in horses. In conclusion, HK racehorses have low serum 25OHD2 and total 25OHD concentrations and rely on D3 supplementation to maintain adequate vitamin D status. Further study is required to determine the optimal form of dietary vitamin D supplementation for Thoroughbred racehorses.

Neutrophil Gelatinase-Associated Lipocalin in Synovial Fluid from Horses with and without Septic Arthritis

Neutrophil gelatinase-associated lipocalin (NGAL) has been suggested to be a highly sensitive and specific marker of joint infection in humans. The aim of the study was to investigate NGAL concentrations in synovial fluid (SF) from horses with septic synovitis, horses without septic synovitis, and horses with uncertain status. NGAL was measured in 177 admission samples obtained from 152 horses. From a subset of horses (n = 35), additional samples obtained sequentially over the course of treatment were available. Concentrations of NGAL were significantly higher in septic synovitis (n = 47 samples) than in samples classified as non-septic (n = 103) or samples with uncertain status (n = 27), with median NGAL concentrations in the three groups being 1236, 16.8, and 266.4 µg/L, respectively. NGAL discriminated nearly perfectly between septic and non-septic (area under the receiver operating characteristic curve 0.98, 95% confidence interval 0.95-1.00). The optimal cut-off value for maximal sensitivity (87.2%) and specificity (75.0%) to discriminate septic samples from those with uncertain status was 444.6 µg/L, with an area under the receiver operating characteristic curve of 0.85 (95% confidence interval 0.74-0.93). Concentrations declined over time in horses undergoing treatment. NGAL is a novel biomarker that seems to have great potential for identifying septic synovitis and for monitoring the response to treatment of synovial infection in horses.

217 Acceptability of Hempseed Meal in Horses

Abstract Congress passed US Farm Bills legalizing hemp (Cannabis sativa L.), after which hemp-farming increased, resulting in an abundance of hemp by-products, like hempseed meal (HSM). As such, HSM must be evaluated as a potential animal feedstuff. A study was designed to evaluate the acceptability of HSM over time in horses, using 6 stock-type geldings (11.5 ± 3.4yr, 539.6 ± 31.9kg, BCS 6). Horses were offered 1 kg of HSM pellets over two 5-min periods, 1 h apart each day, and offered 1 kg of Coastal Bermudagrass (Cynodon dactylon) hay between offerings. Horses remained on a commercial ration during a 1-d feeding-pattern acclimation prior to the 6-d trial. Data (mean ± SEM) were analyzed using a Repeated Measures ANOVA within R Statistical Program. One horse was removed from the study due to complete diet refusal. Intake of HSM on d1 (0.287 ± 0.081kg; P = 0.062), d2 (0.378 ± 0.084kg; P = 0.07), and d3 (0.446 ± 0.087; P = 0.077) tended to be lower than d5 (0.695 ± 0.071 kg). Similarly, d1 (P = 0.043), d2 (P = 0.036), and d3 (P = 0.048) were less than d6 (0.652 ± 0.064 kg). Day 4 (0.530 ± 0.09 kg) tended to be less than d5 (P = 0.09), but there was no difference between d5 and d6 (P = 0.73). Hay consumption was greater on d1 (0.702 ± 0.026kg; P = 0.003) and d2 (0.723 ± 0.041kg; p = 0.001) than d6 (0.410 ± 0.051kg). Hay consumption and HSM intake were not correlated (R2 = 0.001), but the proportions of HSM consumed between feedings were highly correlated (R2 = 0.947), increasing at 0.4 of Feeding 1. These findings indicate that HSM consumption increases after 4 days of exposure but is not impacted by hay consumption.

Single-cell gene expression analysis of cryopreserved equine bronchoalveolar cells.

The transcriptomic profile of a cell population can now be studied at the cellular level using single-cell mRNA sequencing (scRNA-seq). This novel technique provides the unprecedented opportunity to explore the cellular composition of the bronchoalveolar lavage fluid (BALF) of the horse, a species for which cell type markers are poorly described. Here, scRNA-seq technology was applied to cryopreserved equine BALF cells. Analysis of 4,631 cells isolated from three asthmatic horses in remission identified 16 cell clusters belonging to six major cell types: monocytes/macrophages, T cells, B/plasma cells, dendritic cells, neutrophils and mast cells. Higher resolution analysis of the constituents of the major immune cell populations allowed deep annotation of monocytes/macrophages, T cells and B/plasma cells. A significantly higher lymphocyte/macrophage ratio was detected with scRNA-seq compared to conventional cytological differential cell count. For the first time in horses, we detected a transcriptomic signature consistent with monocyte-lymphocyte complexes. Our findings indicate that scRNA-seq technology is applicable to cryopreserved equine BALF cells, allowing the identification of its major (cytologically differentiated) populations as well as previously unexplored T cell and macrophage subpopulations. Single-cell gene expression analysis has the potential to facilitate understanding of the immunological mechanisms at play in respiratory disorders of the horse, such as equine asthma.

Feeding time in horses provided roughage in different combinations of haynets and on the stable floor

Restrictions in forage availability lead to short eating or foraging times which could contribute to reduced welfare in horses. Strategies to maximise foraging time are therefore of interest. One such strategy is to feed forage in small-holed haynets to restrict eating rate, but it can also induce frustrating behaviours in the horse. Other strategies are therefore of interest. The effect of adding straw to a forage-based diet, with and without the use of haynets, on eating and feeding time in horses was examined in this study. Combinations examined were: haylage on the floor and no straw; haylage in haynet and no straw; haylage and straw in the same haynet; and haylage in haynet and straw on the floor. Eight horses were used in a complete change-over study where each treatment lasted for three consecutive days and horses were observed for 2 h during each treatment day. Measurements included forage eating time (minutes per kg dry matter forage), forage eating rate (kg dry matter forage per h), and total feeding time (in minutes per observation period, including effective eating time and feed searching time). Average forage eating time was shorter (P = 0.0004) and average forage eating rate was faster (P = 0.0001) when haylage was fed on the floor with no straw available, compared to all other treatments. Average total feeding time was longer (P < 0.03) for treatments where straw was included compared to treatments without straw. Results showed that feeding forage in haynets prolonged forage eating time and slowed down forage eating rate compared to feeding forage on the floor. The total feeding time was extended by providing straw on the floor in addition to forage in a haynet, or together with the forage in the same haynet. In conclusion, providing straw in addition to forage with or without using a small-holed haynet increased total feeding time and feed searching time compared to treatments without straw

The potential of three whole blood microRNAs to predict outcome and monitor treatment response in sarcoid-bearing equids

MicroRNAs (miRNAs) have been proposed as biomarkers for equine sarcoid (ES) disease. In this study, the suitability of three whole blood miRNAs to diagnose ES and to predict and monitor the outcome of therapy was explored. Using reverse transcription-quantitative polymerase chain reaction (RT-qPCR), expression levels of eca-miR-127, eca-miR-379, and eca-miR-432 in whole blood of ES-affected equids before and at least one year after therapy were compared to those of unaffected control equids. Associations of age, sex, species, diagnosis, and therapy outcome with miRNA expression levels were examined using general linear models. In total, 48 ES-affected equids and 47 control equids were recruited. From the affected animals, 31 responded favorably to treatment, and 17 demonstrated a failure of therapy. None of the tested miRNAs were influenced by age. Male equids showed increased expression of eca-miR-127 compared to females and horses showed higher expression levels of eca-miR-379 and eca-miR-432 than donkeys. Eca-miR-127 was confirmed as a diagnostic discriminator between ES-affected and control equids. No difference in miRNA profiles before therapy was found when comparing ES-affected equids with success vs. failure of therapy. Eca-miR-379 and eca-miR-432 decreased over time in horses where therapy was successful, but not in those cases where it failed. Biological variables influence equine whole blood miRNA expression, which may complicate biomarker validation. While none of the tested miRNAs could predict the response to therapy in ES-affected equids and eca-miR-127 showed poor diagnostic accuracy for ES, eca-miR-379 and eca-miR-432 miRNAs might allow refinement of monitoring of success of ES therapy.

Continuous measurement of arterial oxygenation in mechanically ventilated horses.

The possibility of accurately and continuously measuring arterial oxygen partial pressure (PaO2 ) in horses may facilitate the management of hypoxaemia during general anaesthesia. The aim of this study was to evaluate the ability of a novel fibreoptic sensor to measure PaO2 (PaO2Sensor ) continuously and in real time in horses undergoing ventilatory manoeuvres during general anaesthesia. In vivo experimental study. Six adult healthy horses were anaesthetised and mechanically ventilated in dorsal recumbency. A fibreoptic sensor was placed in one of the facial arteries through a catheter to continuously measure and record PaO2Sensor . After an alveolar recruitment manoeuvre, a decremental positive end-expiratory pressure (PEEP) titration using 20-minute steps of 5cmH2 O from 20 to 0cmH2 O was performed. An arterial blood sample was collected at 15minutes of ventilation at each PEEP level for PaO2 measurement using an automated blood gas machine (PaO2Ref ). The agreement between PaO2Sensor and PaO2Ref was assessed by Pearson's correlation, Bland-Altman plot and four-quadrant plot analysis. In the last minute of ventilation at each PEEP level, a slow tidal inflation/deflation manoeuvre was performed. The mean relative bias between PaO2Sensor and PaO2Ref was 4% with limits of agreement between -17% and 29%. The correlation coefficient between PaO2Sensor and PaO2Ref was 0.98 (P<.001). The PaO2Sensor and PaO2Ref concordance rate for changes was 95%. Measurements of PaO2Sensor during the slow inflation/deflation manoeuvre at PEEP 15 and 10cmH2 O were not possible because of significant noise on the PaO2 signal generated by a small blood clot. Small sample size. The tested fibreoptic probe was able to accurately and continuously measure PaO2Sensor in anaesthetised horses undergoing ventilatory manoeuvres. A heparinised system in the catheter used by the fibreoptic sensor should be used to avoid blood clots and artefacts in the PaO2 measurements.

Outcomes following single, caudally based bilateral versus unilateral frontonasal sinusotomy for treatment of equine paranasal sinus disease

BackgroundBilateral sinus disease is relatively uncommon in horses, accounting for 3%–4.5% of horses with sinonasal disease, but may require bilateral paranasal surgery for complete resolution. Complications and recurrence following bilateral sinusotomy have not been reported or compared to those following unilateral procedures.ObjectiveTo describe clinical features and outcomes in horses undergoing standing single, caudally based bilateral frontonasal sinusotomy compared to unilateral frontonasal surgery.MethodsRecords of horses (n = 37) undergoing surgical treatment for sinus disease (five bilateral, 32 unilateral) were retrospectively reviewed (2010–2017) for signalment, presenting complaint, duration of signs preoperatively, diagnostic imaging, treatments administered, duration hospitalization, complications, and owner satisfaction with the procedure. Mann–Whitney testing was used to compare age, duration of hospitalization, and follow‐up time in horses undergoing unilateral or bilateral procedures. Fisher's exact testing was used to determine if sex predilection was present for unilateral or bilateral disease. Survival time and time to recurrence were compared by Kaplan–Meier survival curves and log‐rank curve comparison testing. Significance was assessed at p < 0.05.ResultsLength of signs prior to admission did not differ between horses with unilateral and bilateral disease (p = 0.09), but there was a tendency for horses with bilateral disease to have clinical signs for longer. Age (p = 0.19) and hospitalization duration (p = 0.53) did not differ between horses undergoing unilateral versus bilateral procedures. Recurrence or failure to resolve signs was reported in 11/32 (34%) of unilateral and 0/5 bilateral cases (p = 0.07).ConclusionsThe bilateral single, caudally based sinusotomy approach may be considered to effectively treat bilateral paranasal sinus disease without concern for increased risk of life‐threatening complications or longer hospitalization duration than would be typical for unilateral sinusotomy procedures.

Emergence and Spread of Different ESBL-Producing Salmonella enterica Serovars in Hospitalized Horses Sharing a Highly Transferable IncM2 CTX-M-3-Encoding Plasmid.

Salmonella enterica is a major causative pathogen of human and animal gastroenteritis. Antibiotic resistant strains have emerged due to the production of extended-spectrum β-lactamases (ESBLs) posing a major health concern. With the increasing reports on ESBL-producing Enterobacterales that colonize companion animals, we aimed to investigate ESBL dissemination among ESBL-producing Salmonella enterica (ESBL-S) in hospitalized horses. We prospectively collected ESBL-S isolates from hospitalized horses in a Veterinary-Teaching Hospital during Dec 2015–Dec 2017. Selection criteria for ESBL-S were white colonies on CHROMagarESBL plates and an ESBL phenotypic confirmation. Salmonella enterica serovars were determined using the Kaufmann-White-Le-Minor serological scheme. ESBL-encoding plasmids were purified, transformed and compared using restriction fragment length polymorphism (RFLP). Whole genome sequencing (Illumina and MinION platforms) were performed for detailed phylogenetic and plasmid analyses. Twelve ESBL-S were included in this study. Molecular investigation and Sequence Read Archive (SRA) meta-analysis revealed the presence of three unique Salmonella enterica serovars, Cerro, Havana and Liverpool, all reported for the first time in horses. PFGE revealed the clonal spread of S. Cerro between seven horses. All twelve isolates carried blaCTX–M–3 and showed an identical multidrug resistance profile with co-resistance to trimethoprim/sulfamethoxazole and to aminoglycosides. Plasmid RFLP proved the inter-serovar horizontal spread of a single blaCTX–M–3-encoding plasmid. Complete sequence of a representative plasmid (S. Havana strain 373.3.1), designated pSEIL-3 was a -86.4 Kb IncM2 plasmid, that encoded nine antibiotic resistance genes. pSEIL-3 was virtually identical to pCTX-M3 from Citrobacter freundii, and showed high identity (>95%) to six other blaCTX–M–3 or blaNDM–1 IncM2 broad host range plasmids from various Enterobacterales of human origin. Using a specific six gene-based multiplex PCR, we detected pSEIL-3 in various Enterobacterales species that co-colonized the horses’ gut. Together, our findings show the alarming emergence of ESBL-S in hospitalized horses associated with gut shedding and foal morbidity and mortality. We demonstrated the dissemination of CTX-M-3 ESBL among different Salmonella enterica serovars due to transmission of a broad host range plasmid. This report highlights horses as a zoonotic reservoir for ESBL-S, including highly transmissible plasmids that may represent a ‘One-Health’ hazard. This risk calls for the implementation of infection control measures to monitor and control the spread of ESBL-S in hospitalized horses.

Generation of myostatin edited horse embryos using CRISPR/Cas9 technology and somatic cell nuclear transfer

The application of new technologies for gene editing in horses may allow the generation of improved sportive individuals.Here, we aimed to knock out the myostatin gene (MSTN), a negative regulator of muscle mass development, using CRISPR/Cas9 and to generate edited embryos for the first time in horses. We nucleofected horse fetal fibroblasts with 1, 2 or 5 µg of 2 different gRNA/Cas9 plasmids targeting the first exon of MSTN. We observed that increasing plasmid concentrations improved mutation efficiency. The average efficiency was 63.6% for gRNA1 (14/22 edited clonal cell lines) and 96.2% for gRNA2 (25/26 edited clonal cell lines). Three clonal cell lines were chosen for embryo generation by somatic cell nuclear transfer: one with a monoallelic edition, one with biallelic heterozygous editions and one with a biallelic homozygous edition, which rendered edited blastocysts in each case. Both MSTN editions and off-targets were analyzed in the embryos. In conclusion, CRISPR/Cas9 proved an efficient method to edit the horse genome in a dose dependent manner with high specificity. Adapting this technology sport advantageous alleles could be generated, and a precision breeding program could be developed.

Modeling digesta mean retention time in horses using a 2-compartment model

Modeling digesta mean retention time in horses using a 2-compartment model

Using Movement Sensors to Assess Lying Time in Horses With and Without Angular Limb Deformities

Chronic musculoskeletal pathologies are common in horses, however, identifying related effects can be challenging. This study tested the hypothesis that movement sensors and analgesics could be used in combination to confirm the presence of restrictive pathologies by assessing lying time. Four horses presenting a range of angular limb deformities (ALDs) and four nonaffected controls were used. The study comprised two trials at separate paddock locations. Trial A consisted of a 3-day baseline phase and 2 × 3-day treatment phases, during which two analgesics were administered to two ALD horses and two controls in a standard crossover design. Trial B replicated trial A, except that as no difference between analgesics had been evident in trial A, only one analgesic was tested. Movement sensors were used to measure the horses' lying time and lying bouts. In trial A, ALD horses' basal mean lying time was significantly less than controls (means ± SD for ALD horses 213 ± 1.4 minutes and for controls 408 ± 46.7 minutes, P = .007); with analgesic administration, the difference became nonsignificant. In trial B, ALD horses' basal mean lying time was also significantly less than controls (ALD horses 179 ± 110.3 minutes; controls 422.5 ± 40.3 minutes, P < .001), again becoming nonsignificant with analgesic administration. Given the increases in ALD horses' lying time with analgesic administration, it is possible that their shorter basal lying time is associated with musculoskeletal discomfort. Despite the small sample size, movement sensors effectively measured this behavior change, indicating that they could be a useful tool to indirectly assess the impact of chronic musculoskeletal pathologies in horses.

Prevalence of Gastro-Intestinal Parasites of Horse (Equus Caballus Linnaeus, 1758) In Seven Village Development Committee of Rukum District, Nepal

This study aimed to determine the prevalence of gastro-intestinal parasites of horse in seven Village Development Committee (VDC) of Rukum district. It was carried out from March to November 2016. A total of 105 fecal samples of horses (79 males and 26 females) were collected by using opportunistic random method. The collected fecal samples were preserved in 2.5% potassium dichromate and microscopically examined using concentration techniques. The overall prevalence of gastro-intestinal parasites was 84.76% (89/105). The total number of genera observed during fecal examination was 12. Among them, Strongylus sp. showed the highest prevalence (51.42%) followed by Eimeria sp. (20%), Trichostrongylus sp. (14.28%), Trichonema sp. (13.33%), Parascaris equorum (10.47%), Balantidium sp. (9.52%), Dictyocaulus sp. (8.57%), Triodontophorus sp. (7.61%), Gastrodiscus sp. (6.66%), Oxyuris equi (4.76%), Entamoeba sp. (3.80%), Shistosoma sp. (1.90%) and unidentified nematode larvae (7.61%). Three genera of parasites; Shistosoma sp., Triodontophorus sp. and Dictyocaulus sp. have been reported for the first time in horses of Nepal. Present study showed higher infection rate in females (92.30%) than in male horses (82.27%). No significant association was observed between the prevalence of parasite rate with VDC (χ2 = 5.7161; p&gt;0.05) and sex of animals (χ2 = 0.3346; p&gt;0.05).Journal of Institute of Science and Technology Volume 22, Issue 2, January 2018, Page: 70-75

Influence of Anticoagulants on Platelet and Leukocyte Concentration From Platelet-Rich Plasma Derived From Blood of Horses and Mules

Platelet-Rich Plasma (PRP) is a biological product often used to promote tissue repair, due to its composition of growth factors. The aim of this study was to compare the viability of platelets on horses and mules and the influence of two different anticoagulants—dextrose citrate (ACD) and sodium citrate (CIT)—while obtaining PRP. Eight horses and eight mules were used, with two replicas each. PRP was obtained after single centrifugation at 133 g for 8 minutes. Blood analysis was performed on hematologic analyzer, and morphology was evaluated through optical microscopy of phase inversion. Platelet concentration on PRP (when compared with total blood samples) was 1.42 times in horses and 2.0 times in mules using ACD, and 1.39 times in horses and 2.69 times in mules using CIT. The protocol was more efficient in concentrating mules' platelets when compared with horses', and CIT was responsible for a larger PRP leukocyte reduction on both equids. CIT was a better anticoagulant for mules' PRP procurement, due to its promotion of higher platelet concentration.

Gastrointestinal nematodes of equine in the region of Thessaloniki

During a period of approximately two months (January-February 1995), 91 out of 119 riding horses of the seven riding clubs of Thessaloniki their feces were examined. The purpose of this survey was to find out and identify parasites after examining their reproductive elements. In the feces of the horses of two riding clubs had not been found any reproductive elements, while in the remaining five were found strongylid eggs in population whieh ranged from 2-5 to 85-94 / g of feces; per club ( rate infection 68; 1 %). After the examination of the 3rd stage larval, which obtained from fecal culture, were identified, from large strongylids (sub-family Strongylinae), the species Triontophorus sp (42%), Strongylus equinus (29%), Strongylus edentatus (21 %), Strongylus vulgaris (6%), and Trichostrongylus axei (2%) from the family of Trichostrongylidae. From the small strongylids (sub-family Cyathostominae) were identified the genera Cyathostomum (42%), Cylicostephanus (24%), Gyalocephalus (22%) and Poteriostomum (12%). The identified genera Triontophorus, Gyalocephalus and Poteriostomum as well as the species Trichostrongylus axei, are mentioned for the first time in horses in greek region.

Cytokine Concentrations Over Time In Horses With Acute Abdominal Pain

Cytokine Concentrations Over Time In Horses With Acute Abdominal Pain

The Incidence Of Post‐Operative Colic Over Time In Horses Discharged Following Emergency Exploratory Laparotomy

The Incidence Of Post‐Operative Colic Over Time In Horses Discharged Following Emergency Exploratory Laparotomy