Abstract: In this work, a simulative analysis of 40 Gb/s downstream time and wavelength division multiplexing-passive optical network (TWDM-PON) for next-generation passive optical network second stage (NG-PON2) had been designed and demonstrated. The performance of three different wavelength plans in TWDM-PON are evaluated as the solution to NG-PON2 to support 40Gbit/s bandwidth requirement. A four 10-Gb/s TWDM-PON signals are stacked using four different wavelengths in the downstream direction and externally modulated. The system performance is observed using Bit Error Rate (BER) and Optical Signal-to-Noise Ratio (OSNR) with respect to the design parameters such as distance, number of users and received optical power. Thus, the proposed system was able to support 512 number of users and 80 km transmission distance. When the system is tested upon transmission of triple play services over 80km distance with 512 users, it is shown that BER of 8.9 × 10-10, 3.9 × 10-9 and 1.3 × 10-4 are achieved for data, voice and video, respectively.