Keeping cover crops to reduce nitrogen leaching often conflicts with timing tillage operations before the soil becomes un-trafficable during winter, while leaving cover crops in the field until spring raises concerns over pre-emptive competition with the following crop. Therefore, farmers may resort to tilling their fields in autumn after letting cover crops remain in the fields for only a short period of time. We explore the effects of this practice in a laboratory lysimeter setting by analyzing the leaching of nitrate from intact topsoil cores. Cores were extracted from no-till (NT) plots and plots tilled in autumn (AuT), in areas kept bare (B) and with volunteer winter rye plant cover (V) after harvest. Nitrate breakthrough curves show that V significantly reduced N leaching by 61% relative to B in NT, but did not have a significant effect in AuT. Dissection of leached cores and undisturbed reference cores indicated a significant removal of mineral N from the soil during the lysimeter experiment for all treatments except V in NT. This indicates that volunteer cover removed a crucial amount of leachable N and suggests that tillage counteracted the effect of V in AuT, likely due to a combination of reduced uptake and re-mineralization of N in cover crop residue.