Tilia Platyphyllos Scop Research Articles

Can the galling mite Eriophyes tiliae alter the phenolic profile and antioxidant capacity of Tilia platyphyllos in a high UV radiation environment?

AbstractLeaves of large‐leaved lime, Tilia platyphyllos Scop. (Malvaceae), harbor colonies of Eriophyes tiliae (Pagenstecher) (Acari: Eriophyidae), capable of modifying the leaf structure (inducer of nail‐galls) and physiology. The aerial organs of T. platyphyllos have traditionally been consumed for their high antioxidant capacity, related to the rich polyphenol profile. Here, we investigated the polyphenol profiles and antioxidant activity of T. platyphyllos non‐galled leaves and galls to determine the changes induced by the mite in a Chilean environment with high ultraviolet (UV) radiation. Phenolic compound identification in the methanol extracts of non‐galled leaves and galls was carried out through HPLC. Furthermore, the antioxidant activity of the extracts was quantified through spectrophotometry, and it was compared with the standards of the three major phenolic compounds of both organs. Nine phenolic compounds were detected in non‐galled leaves and galls, with no differences between the two organs, except for the absence of gallic acid in galls. The concentration of phenolic compounds did differ significantly between the two conditions. In leaf galls, epicatechin and cyanidin‐3‐glucoside concentrations and antioxidant capacity increased significantly compared to non‐galled leaves. As chlorogenic acid and cyanidin‐3‐glucoside are active phenols responding to UV radiation stress, their concentration in T. platyphyllos leaves could be a response to the high UV radiation occurring in Chile during spring and summer. The phenolic compounds detected here have been reported to be potent antioxidants, that are probably potentiated by E. tiliae for its own protection against UV‐B radiation, as the two most abundant compounds in the galls exhibited the highest capacity to reduce ABTS and DPPH radicals. However, we do not rule out the participation of phenolics in protection against natural enemies of the gall mite, as chlorogenic acid and epicatechin are powerful antifeedants.

Plants and mental disorders: the case of Catalan linguistic area.

Introduction: Mental disorders are among the leading causes of ill-health and disability worldwide. Despite the disease burden they cause, including significant direct and indirect impacts on individual's health and major social and economic consequences in all countries of the world, it is still one of the most neglected areas of public health. In such a context, the medicinal plants traditionally used to pale these pathologies are presented as a promising tool for future drug development for the management of mental health disorders. The aim of the present study is to analyze the information about plant species used to treat mental disorders in the Catalan linguistic area (CLA) and compare these traditional uses with pharmacological literature in order to evaluate the most quoted taxa and their uses and to provide a basis for further research. Methods: Data have been recovered from the "Etnobotànica dels Països Catalans" webpage (https://etnobotanica.iec.cat/) and the meta-analytic work carried out in the present study covers 27 prospections performed in different territories between 1990 and 2019. Descriptive statistics and quantitative ethnobotany were carried out and some ethnobotanical indices were calculated. Results and Discussion: The number of use reports analysed to treat mental disorders in CLA is 2,544 spread over 183 taxa belonging to 64 families, being the most cited the Malvaceae (29.36% of use reports), Lamiaceae (16.71%), Caprifoliaceae (7.94%), Rutaceae (7.47%) and Papaveraceae (6.01%). The most used taxa to treat or alleviate the mental disorders have been Tilia platyphyllos Scop. (24.53%), Valeriana officinalis L. (7.47%), Salvia officinalis L. (5.07%), Sambucus nigra L. (4.28%), and Ruta chalepensis L. (3.89%). The flowers or inflorescences (47.68%), followed by aerial part (23.49%), have been the most used plant parts, and tisane the most commonly used pharmaceutical form (78.03%). The most reported use is as sedative with 40.92%, followed by anticephalalgic (21. 19%) and tranquilizer (20.01%). The informant consensus factor (FIC) was 0.93, and 3.72% was the ethnobotanicity index (EI) value. The information is coincidental with at least one of the comprehensive pharmacological literature sources checked for 73.68% of ethnobotanical uses.

Total secondary metabolites and heavy metal profile of some medicinal plants frequently consumed as winter tea

In this study, sage (Salvia officinalis L.), linden (Tilia platyphyllos Scop.), and daisy (Matricaria chamomilla L.) herbs, which have rich phytochemical content and are frequently consumed in winter months for medicinal purposes, were obtained from three different herbalists located in Bingol and investigated in terms of their heavy metal contents, total flavonoid, total anthocyanin and total polyphenol contents. Some of the heavy metal (Cr, Fe, Co, Ni, Cu, Zn, As, Hg, Pb) contents of the collected herb samples were determined by ICP-MS. Total flavonoid, total anthocyanin and total polyphenol contents were determined by analyzing spectrophotometric methods. While the total phenolic content was found the most in sage (S1) ethylacetate and methanol extracts (760.1±2.0, 410.33±1.5 mg GAE/g), it was found the least in linden (L1, L3) hexane extracts (6.66±.1.3, 8.35±0.8 mg GAE/g). It was determined that total flavonoid content and total anthocyanin contents were higher in sage (S1) ethylacetate (294.31±2.1 mg CE/g and 291.31±2.1 mg MvGE/g), sage (S2) methanol (375.0±1.9 mg CE/g and 139.01*±2.0 mg MvGE/g) extracts, and less in linden (L1) and daisy (D1) hexane extracts respectively. It was determined that Cr and Cu metals were among the values suitable for consumption in terms of health in all samples except the chamomile (D1, D3) samples, and Zn metals were among the values suitable for consumption in all samples except the linden (L2) sample. Furthermore, the toxic metal Cd (0.05±0.00- 0.09±0.00 μg/g) in daisy (D1, D3) samples, as well as the other toxic metal Pb (4.50±0.01-6.43±0.01 μg/g) in sage (S1,S2,S3) and linden (L1,L2,L3) samples, were found to be among the values suitable for consumption in terms of health. As a result, when the total anthocyanin, polyphenol and flavonoid contents were compared in all groups it was found that sage had the highest value in ethyl acetate extract. Additionally, Cu and Ni values of sage, and Fe and Pb values in both sage and linden were within safe limits in terms of health. They are of utmost importance in terms of supporting the daily mineral intake. However, As, Pb, and Hg were found to be toxic in daisy extract. This result can be shown as a result that the daisy will be a bioaccumulator. Therefore, consumption of daisy tea should be limited.

The experience of preserving ancient trees in Vienna, Austria

Ancient trees hold considerable aesthetic and ecological value within urban environments. The examination of ancient trees and their overall health and condition is an integral part of a comprehensive assessment aimed at preserving these trees and conserving the natural environment within urban spaces. The purpose of the study was to examine the experience of preserving valuable trees growing in urban space. General scientific theoretical methods – analysis and synthesis – were used to work with lists of tree inventory. Heritage trees grow in all districts in Vienna. It was established that ancient trees were given the status of natural monuments since 1936, the dynamics of bequests were traced, and the decades of Vienna’s most active nature conservation activities were determined. The most famous heritage trees in the city were described. The species diversity of monumental trees was analysed, and their taxonomic affiliation was summarised. The heritage trees in Vienna are represented by 49 genera, most of which belong to the phylum of Magnoliophyta. The most common species among protected trees are Platanus orientalis L., Pinus nigra J.F. Arnold, Aesculus hippocastanum L., Quercus robur L., Taxus baccata L., Tilia platyphyllos Scop. Most of heritage trees (60%) are solitary trees. It was evaluated that the general sanitary condition of protected trees is excellent; almost all trees have high vitality and play a leading role in shaping urban landscapes. Every heritage tree possesses a commendable and elevated aesthetic rating. Analysis of the existing inventory of ancient trees in Vienna, their sanitary condition and aesthetic condition will contribute to the environmental awareness of communities and the improvement of management measures for such trees. The obtained results can be used to share the experience of researchers from different countries regarding preserving ancient trees and forming European databases of heritage trees

Volatile compositions of Tilia platyphyllos Scop. infusions by headspace-solid-phase microextraction (HS-SPME), antioxidant activity

The genus Tilia (Tiliaceae) represents 45 species, of which six species are European. Tilia sp. are simple, cordate or deciduous trees with long, silicate shaped, 5-valve fruits and fragrant flowers. It is used as a medicinal tea in traditional medicine for colds, coughs, and hypertension and as an antioxidant. Within the scope of this research, Tilia platyphyllos Scop. in the culture form from Eskişehir was obtained, and infusions were prepared at different times (including 5 min., 10 min., 15 min., and 30 min., respectively). Headspace-solid-phase microextraction (HS-SPME) combined with gas chromatography-mass spectrometry (GC-MS) was used to examine the volatile components in the infusion extracts. Major volatile compounds of infusion extracts determined as E)-β-ocimene (15.7-45.9%) and limonene (11.7-33.4%), respectively. Also, terpinolene (26.0%) were identified as the main compounds for 30 min infusion. After then, the infusion extracts were lyophilized, and the antioxidant activity of the infusion extracts were performed by DPPH· radical scavenging effect. It was determined that inhibition percentages were relatively high in the concentration range of 10-0.02 mg/mL (5.60-72.45%). Our first research was the chemical composition and biological activity of the time-dependent T. platyphyllos infusions.

Application of Artificial Neural Network for Predicting the Drying Kinetics and Chemical Attributes of Linden (Tilia platyphyllos Scop.) during the Infrared Drying Process

This study analyzes the possibility of utilizing artificial neural networks (ANNs) to characterize the drying kinetics of linden leaf samples during infrared drying (IRD) at different temperatures (50, 60, and 70 °C) with sample thicknesses between 0.210 mm and 0.230 mm. The statistical parameters were constructed using several thin-layer models and ANN techniques. The coefficient of determination (R2) and root mean square error (RMSE) were utilized to evaluate the appropriateness of the models. The effective moisture diffusivity ranged from 4.13 × 10−12 m2/s to 5.89 × 10−12 m2/s, and the activation energy was 16.339 kJ/mol. The applied Page, Midilli et al., Henderson and Pabis, logarithmic, and Newton models could sufficiently describe the kinetics of linden leaf samples, with R2 values of >0.9900 and RMSE values of <0.0025. The ANN model displayed R2 and RMSE values of 0.9986 and 0.0210, respectively. In addition, the ANN model made significantly accurate predictions of the chemical properties of linden of total phenolic content (TPC), total flavonoid content (TFC), DPPH, and FRAP, with values of R2 of 0.9975, 0.9891, 0.9980, and 0.9854, respectively. The validation of the findings showed a high degree of agreement between the anticipated values generated using the ANN model and the experimental moisture ratio data. The results of this study suggested that ANNs could potentially be applied to characterize the drying process of linden leaves and make predictions of their chemical contents.

Cellulose in Foliage and Changes during Seasonal Leaf Development of Broadleaf and Conifer Species.

Stable isotope approaches are widely applied in plant science and many improvements made in the field focus on the analysis of specific components of plant tissues. Although technical developments have been very beneficial, sample collection and preparation are still very time and labor-consuming. The main objective of this study was to create a qualitative dataset of alpha-cellulose content of leaf tissues of arboreal species. We extracted alpha-cellulose from twelve species: Abies alba Mill., Acer pseudoplatanus L., Fagus sylvatica L., Larix decidua Mill., Picea abies (L.) Karst., Pinus sylvestris L., Quercus cerris L., Quercus petrea (Matt.) Liebl., Quercus pubescens Wild., Quercus robur L., Tilia platyphyllos Scop. and Ulmus glabra Huds. While these species show an increase in cellulose yield from bud break to full leaf development, the rates of increase in cellulose content and the duration of the juvenile phase vary greatly. Moreover, the veins display significantly higher alpha-cellulose content (4 to 11%) compared to blade tissues, which reflects their different structural and biochemical functions. A guide for the mass of sample material required to yield sufficient alpha-cellulose for a standard stable isotope analysis is presented. The additional benefits of the assessment of the mass of required sample material are reduced sample preparation time and its usefulness in preparing samples of limited availability (e.g., herbarium material, fossil samples).

Фармакологічна корекція стрес-індукованих вегетативних порушень та інсомнії у дітей

У статті наведено механізми розвитку стрес-індукованої вегетативної дисфункції й інсомнії. Обговорюється можливість застосування при даних станах комплексного препарату Сілента. Детально розглядаються фармакодинаміка його компонентів і дані доказової медицини щодо їх застосування. Описано механізми стрес-лімітуючої дії магнію (антагонізм щодо глутаматного рецептора) та L-триптофану (джерело утворення нікотинамідних коферментів, серотоніну й мелатоніну). Комбінація екстрактів квіток ромашки аптечної, листя меліси лікарської, суцвіть липи широколистої гармонізує баланс збудливих і гальмівних процесів у центральній нервовій системі; зменшує психоемоційне напруження, дратівливість, тривожність, страхи, психовегетативні прояви та інсомнію.

Оптимізація протоколу стерилізації експлантатів деяких листяних деревних рослин у культурі in vitro

One of the methods of obtaining planting material of deciduous plants, in particular common ash (Fraxinus excelsior L.), broad-leaved linden (Tilia platyphyllos Scop.) and silver birch (Betula pendula Roth) is microclonal propagation. Asepticity of explants is a prerequisite for microclonal plant propagation. Chemical sterilization with liquid substances is mostly used for this purpose. The mode of decontamination is influenced by a number of factors, in particular the genotype of the plant. The purpose of the study was to optimize the sterilization protocol of F. excelsior, T. platyphyllos and B. pendula explants for microclonal propagation. For research, 20–30 cm of shoots isolated from 12-year-old T. platyphyllos, 10-year-old B. pendula, and 15-year-old F. excelsior in February-March 2021 were used. Plant material was cultured according to conventional methods on a nutrient medium MS (Murashige & Skoog, 1962). Biotechnological methods were used (plant tissue culture in vitro, microclonal propagation). MS Excel software package was used to process the experimental data, the mean and its standard error were calculated. One-way analysis of variance (ANOVA) was performed to analyze the effect of explant sterilization on asepsis. The expediency of keeping plant material during the day in 0.1 % solution of «Samshit»  F. excelsior and 0.3 % solution «Fundazole»  T. platyphyllos and B. pendula is shown. The sterilization protocol of experimental plants (efficiency over 50 %) was optimized by using a stepwise method using 70 % ethyl alcohol, 1.0 % and 2.0 % AgNO3 and 2.5 % and 5.0 % NaClO. The effect of the sterilization regime of experimental plants on asepsis is statistically significant at the level of 5%. To initiate the explants, a culture medium according to the MS prescription was used with the addition of 0.25 mg/L kinetin and 2.0 g/L activated carbon. Further studies are aimed at developing a protocol for direct regeneration of microshoots of F. excelsior, T. platyphyllos and B. pendula under the action of components of the culture medium in vitro.

Dendrocenosis of the Bila Tserkva national agricultural university main building courtyard: diversity, age and condition

The paper reveals the results of taxonomic assessment, phytosanitary monitoring and general condition of the dendrocenosis the BNAU main building the courtyard. It is established that the age structure of plantations ranges from 10 to 80 years, with 60 years old plants to make 12 %. The group of woody plants aged from 41 to 50 years is most numerous (33 % of the total number of trees); the larges number of shrubs ages 16–20 years (6 %). The structure of the dendrocenosis of the courtyard is formed by species of woody plants, the share of their participation ranges from 5.8 to 15 % of the total number of trees: Aesculus hippocastanum L. (15 %), Acer platanoides (9.2 %), Catalpa bignonioides Walt. (7.5 %), Populus tremula L. (6.7 %), Juglans cinerea L. (6.7 %), Ulmus glabra Huds. (6.7 %), Tilia platyphyllos Scop. (5.8 %). According to the life forms of plants, the dendrocenosis is represented by trees (81.7 %) and shrubs (183.%). The taxonomic assessment of the dendrocenosis reveals that that 120 specimens of shrubs are represented by 27 species, grouped into 23 genera of 17 families of 12 orders of 2 divisions. Magnoliophyta predominates in terms of species diversity, which is represented by 24 species from 20 genera of 16 families of 11 orders. According to the number of specimens represented by families, they can be ranked as follows: Sapindaceae Juss. > Juglandaceae DC. ex Perleb> Malvaceae Juss. > Bignoniaceae Juss. > Ulmaceae Mirb. > Salicaceae Mirb. > Oleaceae Hoffmanns. & Link> Anacardiaceae R.Br. > Fabaceae Juss. > Adoxaceae E.Mey. > Paeoniaceae Raf. According to the number of woody plants, the relative living condition of the dendrocenosis is 75 %, which characterizes the weakened condition of the stand of the BNAU main building courtyard. The best living condition was observed in 46 % of the species stand such as Picea abies L., Pinus sylvestris L., Pseudotsuga menziesii (Mirb.) Franco, Fraxinus excelsior L., Styphnolobium japonicum L., Carpinus betulus L., Rhus typhina L., Acer negundo L., Tilia cordata Mill. Under the influence of various biotic and abiotic factors, 26 % of shrubs are in a weakened state, 17 % are severely weakened, 11 % are withered. Key words: dendrocenosis, tree-shrub vegetation, species, genus, family, living condition.

Tree species rather than type of mycorrhizal association drive inorganic and organic nitrogen acquisition in tree–tree interactions

Mycorrhizal fungi play an important role for the nitrogen (N) supply of trees. The influence of different mycorrhizal types on N acquisition in tree-tree interactions is, however, not well understood, particularly with regard to the competition for growth-limiting N. We studied the effect of competition between temperate forest tree species on their inorganic and organic N acquisition in relation to their mycorrhizal type (i.e., arbuscular mycorrhiza or ectomycorrhiza). In a field experiment, we quantified net N uptake capacity from inorganic and organic N sources using 15N/13C stable isotopes for arbuscular mycorrhizal tree species (i.e., Acer pseudoplatanus L., Fraxinus excelsior L., and Prunus avium L.) as well as ectomycorrhizal tree species (i.e., Carpinus betulus L., Fagus sylvatica L., and Tilia platyphyllos Scop.). All species were grown in intra- and interspecific competition (i.e., monoculture or mixture). Our results showed that N sources were not used complementarily depending on a species' mycorrhizal association, but their uptake rather depended on the competitor, indicating species-specific effects. Generally, ammonium was preferred over glutamine and glutamine over nitrate. In conclusion, our findings suggest that the inorganic and organic N acquisition of the studied temperate tree species is less regulated by mycorrhizal association but rather by the availability of specific N sources in the soil as well as the competitive environment of different tree species.

Toxicological and anti-tumor effects of a linden extract (Tilia platyphyllos Scop.) in a HPV16-transgenic mouse model.

Tilia platyphyllos Scop. is a popular broad-leaved tree, native to Central and Southern Europe. Hydroethanolic extracts rich in phenolic compounds obtained from T. platyphyllos Scop. have shown in vitro antioxidant, anti-inflammatory and antitumor properties. The aim of this work was to evaluate the therapeutic properties of a hydroethanolic extract obtained from T. platyphyllos in HPV16-transgenic mice. The animals were divided into eight groups according to their sex and phenotype. Four groups of female: HPV+ exposed to linden (HPV linden; n = 6), HPV+ (HPV water; n = 4), HPV- exposed to linden (WT linden; n = 5) and HPV- (WT water; n = 4) and four groups of male: HPV+ exposed to linden (HPV linden; n = 5), HPV+ (HPV water; n = 5), HPV- exposed to linden (WT linden; n = 5) and HPV- (WT water; n = 7). The linden (Tilia platyphyllos Scop.) extract was orally administered at a dose of 4.5 mg/10 mL per animal (dissolved in water) and changed daily for 33 days. The hydroethanolic extract of T. platyphyllos consisted of protocatechuic acid and (-)-epicatechin as the most abundant phenolic acid and flavonoid, respectively, and was found to be stable during the studied period. In two male groups a significant positive weight gain was observed but without association with the linden extract. Histological, biochemical, and oxidative stress analyses for the evaluation of kidney and liver damage support the hypothesis that the linden extract is safe and well-tolerated under the present experimental conditions. Skin histopathology does not demonstrate the chemopreventive effect of the linden extract against HPV16-induced lesions. The linden extract has revealed a favourable toxicological profile; however, additional studies are required to determine the chemopreventive potential of the linden extract.

Nutlets of Tilia cordata Mill. and Tilia platyphyllos Scop. – Source of bioactive compounds

The aim of this study was to analyse contents of phytosterols, carotenoids, tocochromanols and fatty acid composition in oil from nutlets of linden. Standard determination methods for these compounds were applied using liquid and gas chromatography. In the analysed oils from small and large-leaved lime the dominant acids included linoleic (53.1 and 54.1%), oleic (18.5 and 22.2%), palmitic (15.3 and 11.8%). Linden seed oil was characterised by very high contents of phytosterols (2–2.5 g/100 g). β-Sitosterol was the dominant phytosterol (80–83%). Additionally squalene was detected at 806 and 607 mg/100 g, respectively, for Tilia cordata and T.platyphyllos. It was found that linden seed oil contains tocopherols (93%) and tocotrienols (7%). In terms of tocochromanol contents this oil is surpassed only by wheat germ oil. In terms of the α-T equivalent these oils contain 95 (Tilia cordata) and 50.6 mg/100 g vitamin E (Tilia platyphyllos)

Investigating the Influence of Infrared Drying Method on Linden (Tilia platyphyllos Scop.) Leaves: Kinetics, Color, Projected Area, Modeling, Total Phenolic, and Flavonoid Content.

The Linden (Tilia platyphyllos Scop.) is a highly popular herbal plant due to its central nervous system properties. In this study, thin layer drying kinetics of linden leave samples were experimentally investigated in an infrared (IR) dryer. In order to select the appropriate model for predicting the drying kinetics of linden leaves, eleven thin layer semi theoretical, theoretical, and empirical models, widely used in describing the drying behavior of agricultural products, were fitted to the experimental data. Moreover, the color, projected area (PA), total phenolic content (TPC), and total flavonoid content (TFC) were investigated. The results showed that the drying time decreased from 50 min to 20 min. with increased IR temperature from 50–70 °C. Therewithal, the Midilli model gave the most suitable data for 50 °C, 60 °C. Moreover, Verma et al. and Diffusion approximation models showed good results for 70 °C. The lightness and greenness of the dried linden leaves were significantly changed compared with fresh samples. The PA of dried sample decreased similar to the drying time. In addition, the drying temperature effect on the effective diffusion diffusivity (Deff) and activation energy (Ea) were also computed. The Deff ranges from 4.13 × 10−12 to 5.89 × 10−12 and Ea coefficient was 16.339 kJ/mol. Considering these results, the Midilli et al. model is above the 50 °C, 60 °C, and the Verma et al. and Diffusion to 70 °C, for explaining the drying behavior of linden leaves under IR drying. Moreover, it can be said that the Page model can be used, if it is desired, to express the drying behaviors, partially with the help of a simple equation material by drying. TPC and TFC values were statistically < 0.001 higher in dried samples compared to fresh samples; however, no change has been recorded of TPC and TFC values at different temperatures (50 °C, 60 °C, 70 °C).

Dynamics of mixed lowland forests in Central Bohemia over a 20-year period

The paper deals with the effect of environmental factors and management on various mixed lowland forests in the Medník National Natural Monument, Czech Republic, over a 20-year period. The objectives were to evaluate the structure, production, dynamics and radial growth in relation to climatic conditions in the mixed hornbeam-oak, herb-rich beech and spruce forest stands. The tree density decreased by 8.5% (to 120–1,364 trees·ha&lt;sup&gt;–1&lt;/sup&gt;), while stand volume increased by 28.0% (to 244–767 m&lt;sup&gt;3&lt;/sup&gt;·ha&lt;sup&gt;–1&lt;/sup&gt;) from 1998 to 2018. Large-leaved lime (Tilia platyphyllos Scop.) and Norway spruce (Picea abies /L./ Karst.) showed high variability and sensitivity to climatic factors in radial growth compared to stability and resistance in sessile oak (Quercus petraea /Matt./ Liebl.) and European beech (Fagus sylvatica L.). April, June and July were determined as the most significant months in relation to diameter increment. The synergism of precipitation deficit and high air temperature was a limiting factor of growth in the studied lowland area. The frequency of negative pointer years with extremely low radial growth has been increasing recently. Generally, hornbeam-oak stands are characterized by rich structure, high density and lower productivity, herb-rich beech stands represent rich structured productive forests and spruce forests are very productive stands but with low ecological stability.

Effect of brewing material and various additives on polyphenolic composition and antioxidant bioactivity of commercial Tilia platyphyllos Scop. infusions

Effect of brewing material and various additives on polyphenolic composition and antioxidant bioactivity of commercial Tilia platyphyllos Scop. infusions

Environmental factors influencing expression of bilateral symmetrical traits

Fluctuating asymmetry (FA) is a kind of asymmetry used to assess the stability of development, as the body’s ability to regulate its development on the phylogenetic level. Phenotypic plasticity helps plants to overcome negative effects of temperature variability, and allow to adjusting traits to adverse conditions. The aim of the study is to evaluate the level of plasticity and fluctuating asymmetry in leaf blade under influence of environmental factors. The leaf blades from urban population linden, Tilia spp. located in Moscow region, Russia were used. Fluctuating asymmetry was measured as FA = L – R / (L + R) in absolute value (L and R – left and right value of bilaterally symmetrical metric trait. Plastic variability was measured as PL = 1 – x/X (x and X – smallest and largest value of trait size). When some traits of a leaf blade had a high plasticity, other ones exposed instability of development with an increased FA value. The correlation PL - FA was changeable. In one site correlation r was – 0.69 (p<0.05; 2014; high temperature in May, up to 60% higher norm). The cold and humid vegetative season in 2017 revealed an increase in the plastic dimensions of leaf blades of the broad-leaved linden (Tilia platyphyllos Scop.) with increasing plastic variability of bilateral traits. We attribute results to the phenotypic deviation caused by low temperatures. The predominance of one type of variability was compensating by the weakness of other type variability most likely due to genetic epistasis.

Comparative metabolomics of Tilia platyphyllos Scop. bracts during phenological development

The medicinal plant drug “Tiliae flos” consists of the botanical flowers and bracts of Tilia sp., gathered almost exclusively during flowering. In this study, we examined the changes in the metabolome of specialized products in the bracts of Tilia platyphyllos from the appearance of the organ till the onset of senescence by LC-ESI-MS and data mining.A set of 504 natural products were detected, 241 of which showed significant seasonal variation (p < 9.92E-5). Seven compounds were quantified and an additional 45 were putatively identified. These included flavonoid glycosides, catechins, procyanidins, quinic acid derivatives (including chlorogenic acid) and coumarins. Compared to bracts during flowering, young tissues were characterized by a relatively high diversity of polyphenolic substances. Higher amounts of flavonol glycosides (quercetin, kaempferol), catechins and derivatives have been observed. Deoxyhexosides were almost exclusive to this phenological stage. Changes of about one order of magnitude were not uncommon. For some substances, 5-fold differences were observed (calibration with authentic standards). Some compounds (e.g. the coumarin fraxin) were more prominent at the late fruit growth stage.It was shown that bracts gathered before or after flowering could potentially be therapeutically useful. Changes are rapid during the early phase of bract development: three different groups of compounds presented their maxima during the first 32 days. Considering seasonal variation is of extreme importance during bioactivity tests and screening candidate sources for bioactive natural products. In the case of T. platyphyllos, young and old bracts can be of interest because of their high diversity of distinct specialized metabolites.

Стан локальної популяції Tilia Platyphyllos Scop. та моделювання інвазійності адвентивного виду у парковій зоні міста Дніпро

Зважаючи на континентальний характер регіонального клімату Дніпропетровської області, можна очікувати, що його зміни у напрямку посилення рис аридності мають бути важливим чинником впливу на межі розповсюдження рослинних видів. Зроблено припущення, що деякі деревні рослинні види могли отримати переваги для виживання і розселення на території Степового Придніпров’я в умовах кліматичних змін останніх десятиліть. Досліджено локальні популяції інвазійних адвентивних деревних видів на території парку «Дружби народів», розташованого у лівобережній частині міста Дніпро. Виявлено популяцію насіннєвого походження липи широколистої ( Tilia platyphyllos Scop .) у насадженнях із свіжими гігротопами на лучно-чорноземних ґрунтах із високою трофністю. Розроблено математичну модель розвитку популяцій насіннєвого походження липи широколистої в антропогенно-трансформованих екотопах на території парку «Дружби народів» та прогнозовано збереження тенденції росту інвазійності липи широколистої за умов подальших змін клімату у регіоні.

Vegetative Behaviors of the Linden (Tilia spp.) Seedlings

The main goal of this study was to investigate the morphological development of the seedlings of Tilia spp. The experiments were carried out with seeds from three Tilia species. The seeds were harvested from trees in the region of Plovdiv on 30, 45, 60, 75 and 90 days after flowering. The seeds were sown in soil bed immediately after collecting. The sprouting percentage and percentage of developed and survived seedlings were recorded. In a 3-4 leaves development stage, the morphological characteristics of root, stem and leaves were determined. The seedlings have only developed from 90 days seeds. The differences between species were observed. The highest stem was measured in Tilia tomentosa Moench. The largest root system was developed in the seedlings of Tilia platyphyllos Scop. The seed propagation of linden is most effective by using 90 day old seeds.