Background and objective. An abnormal vaginal discharge and an increase in intestinal aerobic bacteria indicate a vaginal infection. The aim of this study was to assess the antimicrobial susceptibility profiles of bacterial species present in the vaginal microbiota of both non-pregnant and pregnant women. Material and method. A total of 211 vaginal swabs were collected from 120 pregnant and 91 non-pregnant women. The VITEK 2 Compact Automated System validates bacterial isolate diagnosis and antibiotic susceptibility. Results. Out of 120 vaginal samples of pregnant women, 105 samples were identified as Gram-positive (GP) (79) and Gram-negative (GN) (26) while 15 samples were unidentified by VITEK®. The 91 nonpregnant samples comprised identified GP (53), GN (23) and unidentified samples (15). The GP bacteria were highly resistant to Oxacillin (OX1) in both groups, while the GN bacteria were resistant to Ceftazidime (CAZ) and ticarcillin (TIC) in non-pregnant group and TIC in the pregnant groups. The GP bacteria in both groups were sensitive to Tigecycline (TGC). Pregnant and non-pregnant GN bacteria were sensitive to Meropenem (MEM) (61.5%) and Piperacillin/Tazobactam (TZP) (91.3%), respectively. Conclusion. The current study showed that TZP and MEM were both groups' most effective antibiotics against GN isolates. GP bacteria were significantly TGC-sensitive.