Three-way Analysis Of Variance Research Articles

Effect of different beverages and polishing techniques on colour stability of CAD/CAM composite restorative materials

Objectives: The aim of this article was to compare the colour stability of short fibre-reinforced computer-assisted design/computer-assisted manufacturing (CAD/CAM) composite (SFRC CAD) to commercially available CAD/CAM materials following prolonged immersion in a variety of beverages. Furthermore, the influence of the polishing technique was evaluated. Materials and methods: A total of 120 rectangular specimens (10 mm length × 14 mm width × 2 mm thickness) were prepared from SFRC CAD, IPS e-max, Cerasmart 270, Celtra Duo, Enamic, and Brilliant Crios. The specimens underwent polishing through either a laboratory polishing machine equipped with 4000-grit silicon carbide paper or chairside polishing using Sof-Lex spiral. Twenty specimens of each tested CAD/CAM material were randomly divided into four groups (n = 5) based on the staining solution used in order to evaluate the colour stability of the materials. Group 1: distilled water, Group 2: coffee, Group 3: red wine, Group 4: coke. Using a spectrophotometer, the colour changes (∆E) of all CAD/CAM materials were assessed at baseline, and after 1 and 12 weeks of staining. Three-way analysis of variance was used to analyse the data (α = 0.05). Results: The staining solution and material type showed a significant influence on the CAD/CAM specimens’ colour stability (p < 0.05), while polishing method had no significant influence (p > 0.05). The average ∆E values for specimens submerged in wine were considerably higher (p < 0.05) than those for the other solutions. SFRC CAD, Cerasmart 270, and Enamic displayed the highest ∆E values in wine (p < 0.05). Conclusions: The colour stability of tested SFRC CAD was comparable to other composite-based CAD/CAM materials, while IPS e.max exhibited the highest level of colour stability.

Effects of Coloring Liquid Dipping Time and Surface Finishing Procedures on the Optical Properties of Monolithic Zirconia.

Monolithic zirconia restorations can be produced from self-colored or uncolored blocks. The coloring procedure of uncolored blocks and surface treatments may affect the appearance of the restoration. The purpose of this study is to evaluate the effect of liquid coloring and surface treatments on optical properties of translucent monolithic zirconia. All specimens were cut from zirconia blocks with a thickness of 1 mm. Specimens were dipped in the coloring liquids for 3 min, 5 min, and 7 min and then divided into groups of 10 specimens for surface treatments. Glaze and mechanical polishing were applied, and the color of the specimens was measured under the D65 lighting condition with a spectrophotometer device. Color values obtained from the CIE Lab formula and the translucency parameter (TP), opalescence parameter (OP), and contrast ratio (CR) were calculated. The normality of the data was confirmed with the Shapiro-Wilk test. A three-way analysis of variance (ANOVA) was used to assess the effect of dipping time, liquid shade, and surface treatments. The effect of liquid shade and dipping time on the TP, OP, and CR parameters were statistically significant, and the TP values were reduced with the application of coloring liquid. Mechanical polishing groups had higher OP values and lower TP values than glazed groups. In cases where high translucency is required, it may be advisable to use self-colored blocks and apply glaze as a surface treatment to achieve the desired optical properties.

Comparing Various Resin Luting Cement Systems in Different Etching Modes Through Bond Durability and Morphological Features.

This study aimed to investigate bond performance of various resin luting cement (RLC) systems on enamel and dentin in different etching modes and to compare the RLC-tooth interface morphology using scanning electron microscopy (SEM). The self-adhesive RLC systems used in combination with universal adhesives were as follows: Scotchbond Universal Adhesive Plus + RelyX Universal (3M Oral Care) and Clearfil Universal Bond Quick ER + SA Luting Multi (Kuraray Noritake Dental). These RLC systems were also used alone as self-adhesive RLC systems without universal adhesives (self-adhesive mode). The conventional RLC systems for comparison materials were as follows: BeautiBond Xtream + ResiCem EX (Shofu) and Tooth Primer + Panavia V5 (Kuraray Noritake Dental). Twelve specimens were prepared for each group to measure shear bond strength (SBS) in different etching modes. A stainless-steel rod was used as a substitute for indirect restorations. Bonded specimens were allocated to baseline (stored for 24 hours) and artificially aged (thermocycling [TC] for 10,000 cycles) groups. Representative treated tooth surfaces and RLC-tooth interfaces were observed using SEM. Three-way analysis of variance (ANOVA) revealed that all the factors (etching mode, storage period, and RLC system) had a significant influence on the enamel SBS values (p<0.05). Enamel SBS was significantly higher in etch-&-rinse (ER) mode than in self-etch (SE) mode, regardless of RLC system or storage period. Three-way ANOVA showed that all the factors had a significant influence on the dentin SBS values (p<0.001). Most RLC systems showed significantly higher dentin SBS in SE mode than in ER mode, regardless of storage period. However, the combination with Scotchbond Universal Adhesive Plus and RelyX Universal showed no significant difference in SBS values between etching modes at the baseline and showed a significantly higher SBS value in ER mode than in SE mode after TC. The self-adhesive RLC systems with universal adhesives tended to show higher enamel and dentin bond performance than the self-adhesive RLC systems alone. The morphology of treated tooth surfaces and resin cement-tooth interfaces were dependent on the RLC system and etching mode used. The RLC systems with primer application showed a thin, high-density layer above the intact dentin in both etching modes, suggesting chemical interaction.

Bond strength comparison of a fiber-reinforced composite resin: Different dentin conditions and preparation techniques.

This study aimed to compare the bond strength of a fiber-reinforced composite resin with traditional and bulk-fill composite resins under different dentin conditions and preparation techniques. Eighty molar teeth, excluding the mesio-distal half of the occlusal dentin surfaces of each teeth, were isolated with acid-resistant nail varnish and stored in a demineralisation solution (pH 4.5). After mechanical removal of the varnish, the teeth were buried in acrylic resin blocks. In every composite resin group, one-half of the specimens were prepared with a diamond bur and another half with Er: YAG laser. Then, the specimens were divided into four groups of composite resins (Filtek Z250, G-aenial Posterior, SonicFill 2, Ever X Posterior) (n = 10). Shear bond strengths were measured using a universal testing device, and failure types were determined with stereomicroscope images. SEM images were obtained at 1000× magnification. Data were analyzed using a three-way analysis of variance (ANOVA), and Bonferroni correction was used for multiple comparisons (p = .05). Differences in the dentin surface affected the bond strength results (p < .05), whereas there was no significant difference between cavity preparation methods (p > .05). EverX Posterior showed the highest bond strength results. Within the limitations of this study, fiber-reinforced composite resin exhibited successful bond strength results in addition to improved mechanical properties. RESEARCH HIGHLIGHTS: Fiber-reinforced composite had successful bond strength values. Bond strength values of sound dentin groups were higher than those of caries-affected dentin groups. The use of an Er: YAG laser for preparation did not lead to insufficient bond strength results.

Alignment Efficiency of Two Archwires in Adolescents with Moderate to Severe Crowding

There is no consensus in clinical orthodontics on which aligning arch wire should be used at the initial stage of treatment. This randomized clinical trial evaluated the efficiency of multistranded Nitinol (Supercable) aligning arch-wire versus a standard arch-wire (Nitinol) in the first stage of orthodontic treatment in adolescents with severe crowding. Forty patients ages 12 to 17 with an Irregularity Index as proposed by Little between 5 to 17 mm were selected. Vinyl polysiloxane impressions (VPS) were taken before treatment (T1) at 6 weeks (T2) and 12 weeks (T3). Roth prescription .022 x .028 Edgewise brackets were bonded using light cure adhesive to the six anterior teeth of each dental arch and bands were placed on all first molars. Subjects were paired by gender and Irregularity Index into two groups of 20 each and assigned either to the control group (Nitinol) or the experimental archwire (Supercable). The predictor variables in this study were the type of archwire, type of dental arch and the time intervals. The outcome variable was the Irregularity Index score. The Irregularity Index was measured using 3D digital study models (e-models) and GeoDigm software from the VPS impressions taken at T1, T2 and T3. The data was analyzed with both a Two and Three-way Analysis of Variance (p≤0.05) to identify differences in the alignment of the maxillary and mandibular teeth and to determine differences between the two archwires. The Two and Three way ANOVA showed that there was a statistically significant difference (p≤0.05) in the alignment capabilities between the maxilla and the mandible. However, there was no evidence of a statistically significant difference (p=0.301) between NiTi and Supercable archwires at any time interval.

Effect of ceramic type and thickness on the masking ability of chairside machinable all-ceramic crowns.

This study aimed to evaluate the influence of ceramic type and thickness on the masking ability and final aesthetic effects of chairside machinable all-ceramic crowns. Six kinds from three types chairside machinable ceramic materials (IPS e.max CAD HT/MT/LT, IPS Empress LT, and VITA Suprinity HT/T) in shade A2 were fabricated to slice specimens into 1.0, 1.5, and 2.0 mm-thick sections (n=10). The color parameters of the specimens against black and white tiles and four resin substrates (A2, A4, B3, and C4 shade) were measured with a spectrophotometer. The translucency parameter (TP) was calculated using color parameters measured over standard white and black backgrounds. The color differences (ΔE) were calculated between there substrate shades (A4, B3, C4 ) and A2 shade (control group). Two-way analysis of variance (ANOVA) was performed on the TP values. The two va-riables were ceramic type and ceramic thickness. Three-way ANOVA was used to determine the effects of ceramic materials, ceramic thickness, and substrate shades on the ΔE values, followed by Tukey test for multiple comparisons (α=0.05). Ceramic type, ceramic thickness, and substrate shade significantly affected the ΔE values (P<0.001). The L* and b* values of the specimens increased with increasing ceramic thickness, except in substrate A2, whereas the ΔE values decreased. The color difference of all 1.0 mm-thick specimens or all specimens over the substrates C4 shade exceeded the clinically acceptable threshold (ΔE>3.3). The masking ability of chairside machinable all-ceramic crowns is influenced by ceramic type and thickness, and ceramic material. The thickness of ceramic less than 2.0 mm cannot mask the gray shade abutment.

A New Framework for Estimating and Decomposing the Uncertainty of Climate Projections

Abstract Climate projections obtained by running global climate models (GCMs) are subject to multisource uncertainties. The existing framework based on analysis of variance (ANOVA) for decomposing such uncertainties is unable to include the interaction effect between GCM and internal climate variability, which ranks only second to the main effect of GCM in significance. In this study, a three-way ANOVA framework is presented, and all main effects and interaction effects are investigated. The results show that, although the overall uncertainty (O) is mainly contributed by main effects, interaction effects are considerable. Specifically, in the twenty-first century, the global mean (calculated at the grid-cell level and then averaged, and likewise below) relative contributions of all main effects are 54% for precipitation and 82% for temperature; those of all interaction effects are, respectively, 46% and 18%. As the three-way ANOVA cannot investigate the uncertainty components resulting from uncertainty sources, it is improved by deducing the relationship between uncertainty components resulting from uncertainty sources and those resulting from the main effects and interaction effects. By the improved three-way ANOVA, O is decomposed into uncertainty components resulting from the emission scenario (S), GCM (M), and internal climate variability (V). The results reveal that O is mainly contributed by M in the twenty-first century for precipitation, and by M before the 2060s whereas by S thereafter for temperature. The robustness of the V characterization is explored by investigating the variation of V on the number of included ensemble members. The extent of the underestimation of the V contribution is roughly an average of 4% for precipitation and 1% for temperature.

Effect of Dimethyl Sulfoxide Primer on Microtensile Bond Strength and Micromorphological Pattern of HEMA-free Universal Adhesive to Dry/Wet Dentin After Thermomechanical Aging.

To evaluate the effect of dimethyl sulfoxide (DMSO) primer on microtensile bond strength (μTBS) and the micromorphological pattern of a hydroxyethyl methacrylate (HEMA)-free universal adhesive (UA) applied on wet/dry dentin in etch and rinse (E&R) mode before/after thermomechanical aging. For the μTBS test, the mid-coronal dentin of 80 human mandibular first molars was exposed and etched with 35% phosphoric acid. Teeth were randomly divided into two equal groups: dry and wet dentin (n = 40). Then, each group was subdivided according to dentin pretreatment by DMSO before UA (Gluma Bond Universal, Heraeus Kulzer, Hanau, Germany) application into unpretreated and 10% DMSO/water (OT Primer S100, OT Oy Dent, Turku, Finland) pretreated (n = 20). Resin composite blocks were built up using a specially designed Teflon mold. In every subgroup, both the μTBS test and failure analysis by stereomicroscope were evaluated immediately after 24 h and after thermomechanical aging (n = 10). The data were statistically analyzed using a three-way analysis of variance (ANOVA) (p = 0.05). For the micromorphological pattern, 16 maxillary first premolars were distributed as mentioned in the μTBS test, prepared, and buccolingually sectioned. The dentin-resin interface was examined using an environmental scanning electron microscope (ESEM) (n = 2). Three-way ANOVA revealed that the main effects and interactions between dentin wetness, dentin pretreatment, and evaluation time (thermomechanical aging) were not significant for µTBS (p> 0.05). Adhesive failure was the predominant type in all immediate and delayed specimens. Longer and more prominent resin tags were observed at dentin-resin interfaces after DMSO application. Neither the initial dentin wetness condition, dentin pretreatment, nor thermomechanical aging could affect the dentin bond strength. No correlation was found between the bond strength and the micromorphology findings. Key words:Wet/dry dentin bonding, Microtensile bond strength, Micromorphology, Universal adhesive, Dimethyl sulfoxide, Thermomechanical aging.

Filling Material Bond Strength to Dentin Is Positively Influenced by the Agitation of Endodontic Final Irrigating Solutions.

The final step of irrigation has been considered to of increase the bonding strength of filling material to dentin. This study investigated the impact of three final-step irrigation methods on the endodontic sealer bond strength to dentin by using a micro push-out test. Palatal roots of human maxillary molars were cleaned and shaped and randomly divided in six groups (n=15) according to the final-step irrigation method and the type of root canal sealer used. The solutions used for the final-step irrigation were 17% ethylenediaminetetraacetic acid and 2.5% sodium hypochlorite, which underwent three methods: 1) syringe-needle irrigation/conventional, 2) passive ultrasonic irrigation, and 3) XP-endo Finisher agitation. The root canal sealers used were: EndoSequence BC Sealer, and AH-Plus sealer. Roots were obturated with the single cone technique and then, cross-sectioned in 2-mm-thick slices (3 slices from each root). Push-out test was performed on the sliced specimens (cervical, middle, and apical thirds) with a universal testing machine. Bond strength values were recorded in megapascal (MPa). Subsequently, each specimen was longitudinally split to verify the type of failure. Data analysis was performed using Johnson transformation, three-way analysis of variance, Tukey's post-hoc tests, and the partial Eta squared test. There were significant differences in bond strength between the sealers [AH: 4.46±2.24 and BC: 3.47±2.19 MPa (P<0.001)]; between final-step irrigation methods [passive ultrasonic irrigation: 4.52±2.25, XP-endo Finisher: 3.93±3.93 and syringe-needle irrigation/conventional: 3.37±2.51 MPa (P<0.001)], and between the root canal thirds represented by the sliced specimens [cervical: 5.45±2.39, middle: 4.14±1.99 and apical: 2.30±1.30 MPa (P<0.001)]. The interaction between the variables had no significance (P>0.05). Agitation of the final irrigating solution may improve the bonding of the sealer to canal walls. AH-Plus sealer had the highest bond strength. The bond strength reduced significantly towards the apical third.

Light Polymerization through Glass-ceramics: Influence of Light-polymerizing Unit's Emitted Power and Restoration Parameters (Shade, Translucency, and Thickness) on Transmitted Radiant Power.

This in vitro study assessed light transmission through ceramic discs varying in shade, translucency, and thickness using light-polymerizing units with different radiant power/flux (RP) outputs. Disc-shaped specimens (0.5 mm, 1.0 mm, and 2.0 mm) were made from high and low-translucency glass-ceramic ingots (IPS e.max Press) in shades A1 and A4, totaling 60 discs. Two light-polymerizing units with different power outputs were used, and their emission spectra were verified. The transmitted RP values for each ceramic specimen were measured and irradiance and radiant energy influx were calculated. Differences between the light-polymerizing units and the influence of the three ceramic parameters were evaluated using an independent-samples t-test and three-way analysis of variance (ANOVA) tests (α = 0.05). A statistically significant difference was observed in the mean transmitted RP values between the two light-polymerizing units. Furthermore, the three-way ANOVA test showed a significant effect of shade, translucency, and thickness, as well as a significant interaction between each pair of variables and all three variables on the transmitted RP (P < 0.05). Despite the significant attenuation in the transmitted RP, especially in ceramics with higher shade chromaticity and thickness and lower translucency, the calculated minimal irradiance values for both light-polymerizing units (their emitted power ≥ 500 mW) were greater than the minimum recommended irradiance threshold (100 mW/cm2). However, the exposure duration needs to be increased to provide the resin with sufficient radiant exposure for adequate polymerization.

Er:YAG laser settings for debonding zirconia restorations: An in vitro study

This in vitro study aimed to determine the optimal frequency and energy settings for debonding zirconia restorations using an erbium-doped yttrium aluminum garnet (Er:YAG) laser. A total of 200 zirconia specimens (5 mm × 5 mm × 1.5 mm) were fabricated from two types of materials: (1) 3 mol% yttria oxide stabilized tetragonal zirconia polycrystalline (3Y-TZP) and (2) 5 mol% yttria oxide stabilized tetragonal zirconia polycrystalline (5Y-TZP). The zirconia specimens were bonded to dentin using resin cement (RelyX Ultimate, 3 M) and divided into 20 groups based on their laser treatments (n = 5). Er:YAG laser treatment was applied at various frequencies (10 Hz and 20 Hz) and energies (80 mJ, 100 mJ, 120 mJ, 140 mJ, 160 mJ, 180 mJ, 200 mJ, 220 mJ, 240 mJ, and 260 mJ). The time required to debond the specimens and the temperature changes that dentin underwent during the laser treatment were recorded. The surface morphologies of the debonded dentin and zirconia specimens were observed using scanning electron microscopy (SEM). Additional zirconia specimens were fabricated for 4-point flexural strength testing and surface roughness measurements. Statistical analyses were conducted using three-way analysis of variance (ANOVA) and Student–Newman–Keuls (SNK)-q tests (α = 0.05). The debonding time of each specimen varied between 4.8 and 160.4 s, with an average value of 59.2 s. The dentin temperature change for each specimen ranged from 2.3 to 3.6 °C, with an average value of 2.7 °C. The debonding time was significantly influenced by the zirconia material type and laser energy, but it was not affected by the laser frequency. Among the specimens, those made of 3Y-TZP needed significantly more time for debonding than 5Y-TZP. The optimal energies were 220 mJ for 3Y-TZP and 200 mJ for 5Y-TZP. The laser frequency, laser energy, and type of zirconia material had no effect on the dentin temperature change. Additionally, no surface alternations were observed on the dentin or zirconia materials after laser treatment. The surface roughness and flexural strength of the zirconia materials remained unchanged after laser treatment. In summary, Er:YAG laser treatment effectively and safely removes zirconia restorations without impacting their mechanical properties, with a safe temperature change of less than 5.6 °C. The optimum frequency and energy settings for debonding 3Y-TZP and 5Y-TZP restorations were found to be 10/20 Hz and 220 mJ and 10/20 Hz and 200 mJ, respectively.

Influence of hydrothermal aging on the shear bond strength of 3D printed denture-base resin to different relining materials

ObjectivesThis study evaluated the repairability of three-dimensional printed (3DP) denture bases based on different conventional relining materials and aging. Material and methodsThe groups for surface characterization (surface-roughness and contact-angle measurements) were divided based on the denture base and surface treatment. Shear bond strength test and failure-mode analysis were conducted by a combination of three variables: denture base, relining materials, and hydrothermal aging (HA). The initial characterization involved quantifying the surface roughness (n = 10) and contact angle (n = 10) of denture base specimens with and without sandblasting (SB) treatment. Four relining materials (Kooliner [K], Vertex Self-Curing [V], Tokuyama Rebase II (Normal) [T], and Ufi Gel Hard [U]) were applied to 3DP, heat-cured (HC), and self-cured (SC) denture-base resin specimens. Shear bond strength (n = 15) and failure-mode analyses (n = 15) were performed before and after HA, along with evaluations of the fractured surfaces (n = 4). Statistical analyses were performed using a two-way analysis of variance (ANOVA) for surface characterization, and a three-way ANOVA was conducted for shear bond strength. ResultsThe surface roughness peaked in HC groups and increased after SB. The 3DP group displayed significantly lower contact angles, which increased after treatment, similar to the surface roughness. The shear bond strength was significantly lower for 3DP and HC denture bases than for SC denture bases, and peaked for U at 10.65 ± 1.88 MPa (mean ± SD). HA decreased the shear bond strength relative to untreated samples. Furthermore, 3DP, HC, and SC mainly showed mixed or cohesive failures with V, T, and U. K, on the other hand, trended toward adhesive failures when bonded with HC and SC. ConclusionThis study has validated the repairability of 3DP dentures through relining them with common materials used in clinical practice. The repairability of the 3DP denture base was on par with that of conventional materials, but it decreased after aging. Notably, U, which had a postadhesive application, proved to be the most effective material for repairing 3DP dentures.

In Vitro Effect of Mouthrinses on the Microhardness of Three Different Nanohybrid Composite Resins.

Daily use of different mouthrinses at home or in the dental office can alter the properties of resin hardness. The present study aimed to compare Vickers microhardness (VHN) in vitro of nanohybrid composite resins (NCRs) immersed in mouthrinses. In total, 120 discs (10 ⨯ 2 mm) were prepared from three NCR shade A2 (n = 40/group) with FiltekTM Z350 XT (Z350XT), Tetric EvoCeram (TEC), and Polofil NHT (PNHT). The specimens were incubated in artificial saliva (37°C ⨯ 24 hr). Specimens were distributed into four mouthrinses (n = 10/group) of artificial saliva (control), chlorhexidine (CHX 0.12%, pH 5.6), cetylpyridine chloride (CPC 0.075%, pH 5.84), and CHX 0.12% + CPC 0.05% (pH 6.2) (2 times/day, 2' ⨯ 12 hr). The VHN (300 gf/10") was measured after 24 hr, 14, and 21 days. Data were analyzed by three-way analysis of variance, followed by post hoc Tukey analysis at 0.05 level of significance. The results revealed a global effect of the interaction of the mouthrinses ⨯ time between NRC evaluated (P=0.001). Baseline VHN in PNHT and Z350XT was higher than TEC. Within each group of NCR, VHN of CHX + CPC > other mouthrinses (PNHT/14 days; Z350XT/21 days), and >CPC (Z350XT/14 days). In mouthrinses-intragroups, VHN of PNHT and Z350XT decreased at 14 days (CHX, CPC) and was maintained over time in NCR (CHX + CPC). VHN-TEC was similar among groups. Microhardness showed differences due to the interaction of the type of NCR, the composition of the mouthrinses, and time. VHN decreased after 14 days and was more affected in composite resins with lower filler content and in mouthrinses with a lower pH.

Effect of Sodium Ascorbate, Grape Seed Extract, and Aloe Vera Application after In-Office Bleaching on the Bond Strength of Enamel: A 3-Year Evaluation

Introduction. Dental bleaching is the first choice to improve smile esthetics, but, in some cases, it needs to be associated with resin composite restoration to obtain a satisfactory result. Unfortunately, the bonding of resin-based materials can be impaired due to residual oxygen molecules, which can decrease the durability of the restoration. Objectives. To evaluate the effect of the antioxidant application on the bond strength of bleached enamel after 24 hr and 3 years of water storage. Methods. In total, 84 bovine teeth were used in this study. Of these, 77 were bleached with 35% hydrogen peroxide in a single session for three cycles of 15 min. Then, the specimens were divided into groups (n = 7 each): control (without bleaching), without antioxidant (WA) use; application of 10% sodium ascorbate (SA) gel, grape seed (GS) extract, and aloe vera (AV). The restorative procedure was performed immediately after bleaching, 7 and 14 days after bleaching. Specimens were sectioned and evaluated using microtensile bond strength (μTBS). Half of the resin-enamel sticks were tested after 24 hr, and the remaining half after 3 years of water storage. µTBS data were analyzed using a three-way analysis of variance, Tukey’s test, and Dunnett’s test. Results. The lowest µTBS values were observed when the restoration was performed immediately after bleaching in the AV, GS, and WA groups when compared with the SA group ( p &lt; 0.005 ). However, no significant differences were observed among all groups after 3 years of water storage ( p &lt; 0.001 ). Conclusions. SA at 10% was the most effective antioxidant agent for improving the immediate bond strength. However, independent of the antioxidant agent used, the bond strength values were maintained or recovered after 3 years of water storage. SA at 10% could be used to avoid delayed bonding procedures after in-office whitening without compromising bond strength over time. Clinical Significance. The use of antioxidants after dental bleaching can be effective in improving the bonding durability of the adhesive restorations.

Simulation Studies of Three-Way Unbalanced Design on Fixed, Random, and Mixed Model

Analysis of Variance (ANOVA) is a statistical technique used to compare means from various samples. Generally, a balanced design is used in ANOVA, but in some conditions, an unbalanced design can happen when the sample size is different in each treatment. This design will have the calculation of the F-test is different from usual for fixed, random, and mixed models. In this research, a simulation study will be carried out to see the differences in the results of the F-test decision in a three-way ANOVA with an unbalanced design based on a fixed, random, and mixed model. Simulation data is generated based on several scenarios, small sample size and large sample size, e~Normal (0,1) and e~Gamma (2,3), and applied to 8 models, that combine fixed effects and random effects in a 3-factor design. The simulation shows that sample size, error distribution, and the used model can affect F-test decisions. Designs with large sample sizes and e~Normal (0,1) produce more significant F-test decisions than small sample sizes and e~Gamma (2,3), and model 1 or the fixed model has more significant F-test decisions than other models in each scenario.

Verification of EasyGain Settings in the Roger Remote Microphone System.

The purpose of this study was to evaluate how the EasyGain settings on a wireless receiver used with a remote microphone (RM) affected output as a function of hearing loss and receiver type. To assess the effects of the EasyGain setting on a Roger receiver, a Phonak Naida V90 superpower hearing aid (HA), two universal Roger X (02) receivers, two Roger 18 integrated receivers, and a Roger Inspiro transmitter were used. An electroacoustic evaluation was performed for five degrees of hearing loss ranging from a flat 40 to 80 dB HL, and for nine EasyGain settings from +8 to -8 dB with each Roger receiver. A three-way analysis of variance indicated significant main effects of hearing loss, receiver type and EasyGain settings, as well as their two-way interactions (p < .05). Overall, the output of the integrated receiver was 2.77 dB higher than that of the universal receiver. The change in output with 2-dB changes in EasyGain setting varied with the degree of hearing loss. For mild hearing loss (40 and 50 dB), every 2-dB change in EasyGain produced an average of 1.93 dB (SD = 0.17) change in the root-mean-square (RMS) output. For the higher degrees of hearing loss (60, 70, and 80 dB HL), the change in RMS varied depending on the range of EasyGain setting as follows: when EasyGain was > 0 dB, every 2-dB change in EasyGain setting produced an average change in RMS output of 1.32 dB (SD = 0.41); however, when EasyGain was ≤ 0 dB, every 2-dB change resulted in a mean 1.95 dB (SD = 0.16) change. The benefits of EasyGain technology on the RMS output are dependent on the interaction of the receiver type, degree of hearing loss, and EasyGain settings. The results highlight the importance of electroacoustic verification to determine appropriate settings for optimal audibility when using an RM system.

Association between the plasma concentration of melatonin and behavioral temperament in horses

Aggression in horses may engender serious accidents during riding and non-riding activities. Hence, predicting the temperament of horses is essential for selecting suitable horses and ensuring safety during activity. In certain animals, such as hamsters, plasma melatonin concentrations have been correlated with aggressive behavior. However, whether this relationship applies to horses remains unclear. To address this research gap, this study aimed to evaluate differences in the plasma melatonin concentrations among horses of different breeds, ages, and sexes and examine the correlation between plasma melatonin concentrations and the temperament of the horses, including docility, affinity, dominance, and trainability. Blood samples from 32 horses were collected from the Horse Industry Complex Center of Jeonju Kijeon College. The docility, affinity, dominance, and trainability of the horses were assessed by three professional trainers who were well-acquainted with the horses. Plasma melatonin concentrations were measured using an enzyme-linked immunosorbent assay. The consequent values were compared between the horses of different breeds, ages, and sexes using three-way analysis of variance and least significant difference post hoc test. Linear regression analysis was employed to identify the relationship between plasma melatonin concentrations and docility, affinity, dominance, and trainability. The results showed that the plasma melatonin concentrations significantly differed with breeds in Thoroughbred and cold-blooded horses. However, there were no differences in the plasma melatonin concentrations between the horse ages and sexes. Furthermore, plasma melatonin concentrations did not exhibit a significant correlation with the ranking of docility, affinity, dominance, and trainability.

Effects of Different Primers on the Bond Strength to Properly and Excessively Etched Ceramic-coated Zirconia.

To determine the effects of various primers on the immediate and long-term bond strength of ceramic-coated ultratranslucent zirconia that is properly or excessively etched. Ceramic-coated zirconia plates were etched with 9.5% hydrofluoric acid (HF) for 2 or 5 minutes, after which the surface morphology and elemental composition were examined. The etched specimens were treated with different primers including methacryloyloxydecyl dihydrogen phosphate (MDP)-only containing primer, silane-only containing primer, MDP and silane-containing ceramic primer as well as MDP and silane-containing adhesive system. For surface contact angle and shear bond strength (SBS) data, the difference between groups was evaluated by one-way analysis of variance (ANOVA) and three-way ANOVA factorial analysis, respectively. The statistical significance level of 0.05 was set and the Tukey test and the Dunnett-T3 test were used for post-hoc multiple comparisons. Excessive etching (9.5% HF for 5 minutes) led to overconsumption of the glass layer and exposure of zirconia substrate compared to proper etching (9.5% HF for 2 minutes). Among different primers, the surface contact angle of the silane-only containing primer group was the lowest. The silane-only containing primer and MDP and silane-containing ceramic primer produced higher shear bond strength of properly and excessively etched ceramic-coated zirconia, respectively, both before and after thermal aging. The silane-only containing primer and MDP and silane-containing primer can obtain better immediate and long-term shear bond strength for properly and excessively etched ceramic-coated zirconia, respectively.

Effect of shade and opacity on color differences and translucency of resin composite veneers over lighter and darker substrates.

To evaluate color differences (ΔE00) and translucency parameters (TP) from mono, bi, and trilayer resin composite veneers using different opacities and shades of resin composite over lighter and darker simulated tooth-colored substrates. Mono, bi, and trilayer veneers (1.5mm) (n = 12) were made using two shades (A1 and A2) and three opacities (enamel, body, and dentin) of resin composite over simulated lighter (A1) and darker (C4, and C4+) tooth-colored substrates. CIEDE2000 formula was used to calculate ΔE00 considering simulated tooth-colored substrate versus opacities in distinct mono, bi, and trilayer combinations of resin composite over the simulated tooth-colored substrate. The TP was calculated using color coordinates measured over standard white and black backgrounds. Differences in ΔE00 and TP values were calculated with a Three-way Analysis of Variance followed by Tukey's post-hoc test. A1E and A1B monolayer veneers showed similar TP values. Significantly higher ΔE00 values were observed over darker (C4 and C4+), and lower over lighter (A1) simulated tooth-colored substrate. Bilayer and trilayer veneers using dentin opacity provided similar ΔE00 values over the darker tooth-colored substrate. Distinct shades and opacities of resin composite layer combinations over lighter and darker tooth-colored substrates significantly affected TP and ΔE00 values. A1 shade and dentin opacity of monolayer resin composite veneers yielded higher ΔE00 values over darker tooth-colored substrates.

In Vitro Radioenhancement Using Ultrasound-Stimulated Microbubbles: A Comparison of Suspension and Adherent Cell States

Background: Ultrasound-stimulated microbubbles (USMB) have shown potential for enhancing radiation treatment via cavitation and sonoporation mechanisms. However, in vitro studies have produced inconsistent results, with adherent cells demonstrating no radioenhancement. This study aims to investigate the effect of cell adherence on in vitro radioenhancement using USMB and radiation. Method: Lung metastases of follicular thyroid carcinoma cells (FTC-238) and non-small cell lung carcinoma cells (NCI-H727) were treated, both when adhered and in suspension, using 1.6% (v/v) Definity™ microbubbles, ~90 s of 2 MHz ultrasound with mechanical index 0.9, and either 3 Gy or 6 Gy of megavoltage (MV) X-rays. The cell viability was measured using an MTS assay 72 h post-treatment, and statistical analysis was conducted using a three-way analysis of variance. Results: Statistically significant differences were observed for cells treated when adherent compared to suspended. An additive effect was detected in NCI-H727 cells treated in suspension, but not while adherent, while no enhancement was observed for FTC-238 cells in either culture state. Conclusions: To the best of our knowledge, this is the first study to directly compare the effect of cell adherence on the radioenhancement potential of USMB in vitro, and the first to do so using a metastatic cell line.