Three-level Full Factorial Design Research Articles

Development of a Green, Quick, and Efficient Method Based on Ultrasound-Assisted Extraction Followed by HPLC-DAD for the Analysis of Bioactive Glycoalkaloids in Potato Peel Waste.

α-Solanine and α-chaconine are the two most predominant glycoalkaloids (GAs) present in potato. Potato peel contains a high concentration of GAs, which are especially interesting for application in the pharmaceutical industry due to their different beneficial properties (such as anticarcinogenic, anti-inflammatory, antiallergic, antipyretic, antiviral, fungicide, and antibiotic activities, among others); so, potato peel waste can be valorized by extracting these biologically active compounds. For this, a green, quick, and efficient miniaturized analytical approach based on ultrasound-assisted extraction (UAE) combined with HPLC-DAD was developed to quantify α-solanine and α-chaconine in potato peel. Some parameters of the extraction were optimized, including the extraction method, the type of solvent, and the sample/solvent ratio, by a three-factor, three-level (33) full factorial experimental design. The optimal extraction conditions were obtained with UAE using methanol as a solvent and a sample/solvent ratio of 1:10 (w/v, g/mL). The analytical greenness metric for sample preparation (AGREEprep) tool was used to assess the greenness of the methods used. The tool revealed an acceptable green analysis, with 0.61 points. The method was validated and applied to the evaluation of GAs in the peel of 15 commercial varieties of potato. The amount of glycoalkaloids found in the samples evaluated ranged from 143 to 1273 mg/kg and from 117 to 1742 mg/kg dry weight for α-solanine and α-chaconine, respectively. These results reveal the important variability that exists between potato varieties; so, their analysis is of great importance to select the most suitable ones for biovalorization (e.g., the Amandine and Rudolph varieties, with around 3000 mg/kg, in total, of both GAs). To provide higher stability to the peel during storage, freeze-drying or a medium-temperature drying process resulted preferable to avoid GA degradation. Overall, this study will contribute to the expansion of the future biovalorization of potato peel waste as well as provide a powerful analytical tool for GA analysis.

Red Dragon Fruit Peels: Effect of Two Species Ratio and Particle Size on Fibre Quality and Its Application in Reduced-Fat Alpaca-Based Sausages

This research aimed to assess the influence of red dragon fruit peels ratio (RDF-PR) from two species, Hylocereus hybridum (HH) and Hylocereus undatus (HU), and particle size (PS) on quality parameters of red dragon fruit peel powder (RDF-PP) and its further application in emulsified alpaca-based sausages as partial substitutes of pork-back fat. A three-level full factorial design (nine treatments) was employed to evaluate the effect of RDF-PR (HH(0%):HU(100%), HH(50%):HU(50%), and HH(100%):HU(0%)) and PS (499–297, 296–177, and <177 µm) on the dependent variables: L*, a*, b*, C, h°, water-holding capacity, oil-holding capacity, swelling capacity, pectin yield, degree of esterification (analysed through FT-IR), and crude fibre content. The data analysed through a response surface methodology showed that treatment one (T1) is the best with the optimised conditions at 100% HU RDF-PR and PS of <177 µm. The statistical validation of T1 exhibited the highest water-holding capacity (32.1 g/g peel), oil-holding capacity (2.20 g oil/g peel), and pectin yield (27.1%). A completely randomised design (four formulations) was then used to assess the effect of partial replacement of pork-back fat by T1 in emulsified alpaca-based sausages on the colourimetric, physicochemical, and texture properties (hardness, chewiness, cohesiveness, springiness, adhesiveness, and adhesive force). Likewise, a sensory hedonic scale was employed to evaluate the appearance, colour, odour, flavour, texture, and overall acceptability of sausages. The results revealed that 65.7% of pork-back fat content was successfully replaced compared with a control formulation. Additionally, F3 showed significantly (p < 0.05) better colourimetric, physicochemical, and textural characteristics, such as lower hardness (34.8 N) and chewiness (21.7 N) and higher redness (a* = 19.3) and C (22.9), compared to a control formulation. This research presents RDF-PP as a promising fat substitute for developing healthier, reduced-fat meat products using fibre-rich agroindustry by-products.

Influence of surface texture of electric discharge machined Ti6Al4V on the surface wettability

This study examines the effects of crucial ISO 25178 parameters, including height, functional, spatial, functional (stratified), hybrid, and functional (volume), on surface wettability and the influence of key electric discharge texturing process parameters, including current and pulse ON time, on surface roughness (Sa) and water droplet contact angle. To examine the level of significance of each parameter and to determine the level of correlation, a three-level full factorial experimental design, and ANOVA analysis are used. The findings showed that over pulse ON time, the important factor influencing surface roughness (Sa) and contact angle is current. The surface roughness (Sa) is observed to vary between 4.2 and 12.7, whereas the water droplet contact angle varies between 90.7° and 120.4°. In order to find out the correlation between the surface texture parameters based on the ISO 251718, Pearson Correlation analysis were adopted and it is observed that Sa, Sq, Str, Smrk1, Sdr, Vm, and Vmp are having a substantial correlation with the contact angle. Among the ISO25178 parameters examined, Smrk1, however, has demonstrated a highest impact on the contact angle with a highest pearson correlation coefficient of −0.757.

Optimization of ultrasound-assisted extraction (UAE) of (poly)phenolic compounds from blueberry (Vaccinium myrtillus) leaves using full-factorial design

Abstract In this work, the influence of process parameters (temperature: 25 - 65 °C, ethanol content in the extraction solvent: 30 - 90 vol.%, and solid-to-solvent ratio: 1:15 - 1:45 w/v) on the process of ultrasound-assisted extraction (UAE) of (poly)phenols from blueberry leaves (Vaccinium myrtillus) was investigated. Statistical analysis was performed using the MINITAB 21 software, with the application of three-level full factorial designs. The Responses in the study are the content of total (poly)phenols, flavonoids and anthocyanins in the obtained extracts. The extraction of blueberry leaf was significantly (p < 0.05) impacted by process variables. The R2, Adjusted R2, and Predicted R2 values in the study are high, showing a significant relationship between the independent variables and the Response. The optimal temperature for all three Responses is 65 °C, the optimal solid-to-solvent ratio for total (poly)phenols and anthocyanins is 1:45 w/v and for flavonoids is 28.03 w/v, while the optimal ethanol content in the solvent for total (poly)phenols, flavonoids and anthocyanins is 51.21 vol.%, 50.61 vol.% and 83.33 vol.%, respectively.

In Situ Synthesis of Bare Silver Nanoparticles on Paper and Its Response to Copper (II) Ions

This study synthesized bare silver nanoparticles on paper and evaluated its response to copper (Cu(II)) ions to assess its potential as colorimetric sensing platform. The nanoparticles were synthesized in-situ on paper using silver nitrate and sodium borohydride as precursor and reducing agent, respectively. No stabilizer or functionalizing agent was added. A two-factor three-level full factorial design with varying concentration of reagents was employed in the synthesis process. The resulting sensor was successfully characterized using diffuse reflectance spectroscopy and scanning electron microscopy with elemental dispersive x-ray spectroscopy. The sensor was exposed to varying Cu(II) concentrations ranging from 1 to 30 mM and the developed color changes were analyzed using a computer imaging software. The changes in color were quantified using mean gray values from the imaging software. Based on the results, as the concentration of Cu(II) ions increased, the final mean gray value of the paper increased as well. The papers were observed to marginally lighten in color potentially due to the decrease in silver atoms or its interaction with copper. The relationship between Cu(II) concentration and the ratio of final and initial mean gray value was determined and although a weak linear relation existed from 1 to 30 mM, a positive slope supported the increase in mean gray value within the range tested. The changes in the elemental composition of the sensor due to exposure to Cu(II) ions were studied. Finally, the response of the sensor towards other metal ions was compared to that of Cu(II) in a selectivity study.

Design and Optimisation of Sustainable Sample Treatments Based on Ultrasound-Assisted Extraction and Strong Cation-Exchange Purification with Functionalised SBA-15 for Opium Alkaloids in Ground Poppy Seeds.

An analysis methodology was optimised and validated for the quantification of opium alkaloids (OAs) in ground poppy seeds. This involved ultrasound-assisted extraction (UAE) and solid-phase extraction (SPE) purification before analysis using a high-performance liquid chromatography mass spectrometry detector (HPLC-MS/MS). UAE was optimised through the design of experiments with three factors and a three-level full factorial design. For SPE optimisation, a commercial material was compared with a previously synthesised material of SBA-15 silica functionalised with sulfonic groups (SBA-15-SO3-). The synthesised material demonstrated superior efficiency with only 25 mg and proved to be reusable for up to four cycles. The methodology was properly validated in terms of linearity, limits of detection and quantification, and selectivity. Matrix effects were negligible; adequate recovery values (85-100%) and inter-day and intra-day precision (≤15%) were obtained. The greenness of the method was evaluated with the AGREEprep metric scale, being more environmentally friendly compared to OA analysis methods. Finally, the method was applied to different samples of ground poppy seeds and revealed a concentration of 140 mg/kg of morphine equivalents in one of the samples, surpassing the legislatively established limits by sevenfold. This highlights the need to analyse these types of samples to mitigate potential public health issues.

Recycled carpet-reinforced composites from post-consumer polypropylene carpet and recycled HDPE resin

Carpets significantly contribute to landfills, forming 3.5 % of the U.S. landfill waste, with less than 10 % being recycled. This study uses intact carpets to create functional composites as a potential solution to this problem. This research introduces a feasible method for manufacturing recycled composites through compression molding of post-consumer polypropylene (PP) carpet and recycled high-density polyethylene (HDPE) resin. Optimal molding temperature and composition ranges were identified using a comprehensive three-level full factorial design of experiments (3D-DOE), resulting in impressive flexural strength (>40 MPa) and flexural modulus (>2000 MPa). Reproducibility tests of 10 specimens yielded 41.8 ± 2.0 MPa flexural strength and 2200 ± 150 MPa flexural modulus. These composites, containing up to 70 % potentially-landfilled carpet and 30 % recycled resin, surpassed the performance for the strength of commercialized thermoplastics, making them suitable for structural applications. The findings present a promising approach to address carpet landfilling while reducing reliance on additives.

Oily wastewater treatment by a continuous flow electrocoagulation reactor with polarity switch: Assessment of the relation between process variables and the aluminum released to the environment

Electrocoagulation with electrical polarity inversion was used to treat oil in water emulsions (145 ± 5 mg dm−3) using a cylindrical 4.8 dm3 reactor in continuous mode. The effects of spatial time and time between polarity inversion were explored using a three-level full factorial design (32), followed by Spearman correlation (ps), which has shown that the aluminum concentration in the treated effluent is not directly dependent on the mass of aluminum released by the electrodes. Nonetheless, the loss of mass of the electrodes is correlated (ps = 0.6970) to oil removal and to less electric power consumption (ps = −0.6909). Surface response analysis revealed that increasing the number of inversion cycles reduces electrode degradation. The treatment reduced the effluent's chemical oxygen demand by over 92.8%. Regarding environmental impact, there is an inverse statistical correlation between aluminum in the treated effluent and oil removal (ps = −0.7426), indicating that removing more oil with less environmental impact is possible. The better condition, considering oil removal and lower electrode consumption, was obtained with a spatial time of 36 min and a polarity inversion time of 10 s; for this condition, oil removal reached 87.0% with an energy expenditure of about 7.21 kW h.m−3.

Layout Dependence Stress Investigation in through Glass via Interposer Architecture Using a Submodeling Simulation Technique and a Factorial Design Approach.

The multi-chiplet technique is expected to be a promising solution to achieve high-density system integration with low power consumption and high usage ratio. This technique can be integrated with a glass interposer to accomplish a competitive low fabrication cost compared with the silicon-based interposer architecture. In this study, process-oriented stress simulation is performed by the element activation and deactivation technique in finite element analysis architecture. The submodeling technique is also utilized to mostly conquer the scale mismatch and difficulty in mesh gridding design. It is also used to analyze the thermomechanical responses of glass interposers with chiplet arrangements and capped epoxy molding compounds (EMC) during curing. A three-factor, three-level full factorial design is applied using the analysis of variance method to explore the significance of various structural design parameters for stress generation. Analytic results reveal that the maximum first principal stresses of 130.75 and 17.18 MPa are introduced on the sidewall of Cu-filled via and the bottom of the glass interposer, respectively. Moreover, the EMC thickness and through glass via pitch are the dominant factors in the adopted vehicle. They significantly influence the stress magnitude during heating and cooling.

Influence of Polyvinylpyrrolidone Molecular Weight and Concentration on the Precipitation Inhibition of Supersaturated Solutions of Poorly Soluble Drugs.

Supersaturating drug delivery systems such as solid dispersions of a drug in a polymer are frequently used in pharmaceutical development to enable oral delivery of poorly soluble drugs. In this study, the influence of the concentration and molecular weight of polyvinylpyrrolidone (PVP) on the precipitation inhibition of the poorly soluble drugs albendazole, ketoconazole and tadalafil is investigated to expand the understanding of the mechanism of PVP as a polymeric precipitation inhibitor. A three-level full-factorial design was used to delineate the influence of polymer concentration and viscosity of the dissolution medium on precipitation inhibition. Solutions of PVP K15, K30, K60 or K120 at concentrations of 0.1, 0.5 and 1% (w/v), as well as isoviscous solutions of PVP of increasing molecular weight, were prepared. Supersaturation of the three model drugs was induced by the use of a solvent-shift method. Precipitation of the three model drugs from supersaturated solutions in the absence and presence of polymer was investigated by the use of a solvent-shift method. Time-concentration profiles of the respective drugs in the absence and presence of polymer pre-dissolved in the dissolution medium were obtained by the use of a μDISS Profiler™ to determine the onset of nucleation and the precipitation rate. Multiple linear regression was used to evaluate the hypothesis that precipitation inhibition is influenced by the PVP concentration (i.e., the number of repeat units of the polymer) and the medium viscosity of the polymer for the three model drugs. This study showed that an increased concentration of PVP (i.e., an increased concentration of the PVP repeat units, independent of the molecular weight of the polymer) in solution increased the onset of nucleation and decreased the precipitation rate of the respective drugs during supersaturation, which can be explained by an increase in molecular interactions between the drug and polymer with increasing concentrations of polymer. In contrast, the medium viscosity had no significant influence on the onset of the nucleation and precipitation rate of the drugs, which can be explained by solution viscosity having a negligible effect on the rate of drug diffusion from bulk solution to the crystal nuclei. In conclusion, the precipitation inhibition of the respective drugs is influenced by the concentration of PVP, i.e., by molecular interactions between the drug and polymer. In contrast, the molecular mobility of the drug in solution, i.e., the medium viscosity, has no influence on the precipitation inhibition of the drugs.

Direct NIR spectral determination of genetic improvement, light availability, and their interaction effects on chemically selected yerba-mate leaves

Monitoring plant chemical modifications provides useful information about plant genetic variability owing to environmental stresses. This allows determining if the pattern of chemical compounds of interest remains constant. Classically, chemical compounds are monitored through classical analytical procedures that are costly and environmentally harmful. Decaffeinated and high caffeine yerba-mate trees were selected based on their chemical profiles. Their behavior was evaluated at five different shading levels (0%, 40%, 51%, 76%, and 82%), imitating the environmental characteristics of yerba-mate in agroforestry systems. Near-infrared spectroscopy (NIR) fingerprints were obtained for yerba-mate leaves, and ANOVA-simultaneous component analysis (ASCA) models were developed for chemical monitoring. The preprocessing of NIR spectra was assessed with a three-level full factorial design. The best preprocessing set of conditions found was standard normal variate followed by second derivatives, computed according to the Savitzky–Golay method (11-points window and second-degree interpolating polynomial). The ASCA models satisfactorily showed significant differences in chemical behavior for genetic improvement and its interaction with light availibility effects. The genetic improvement effect had a variance of 44.30% over NIR fingerprints, indicating that the chemical differences are maintained at any light level. The interaction effect with 1.84% of the total variance illustrated that the behaviors of decaffeinated and high-caffeinated yerba mate were different when subjected to the same light level. The main absorptions indicated by the ASCA model were the O-H stretching and bending combination band at 1654 nm, and the –CH stretching aromatic 1st overtone at 1675 nm. The ASCA method offered a powerful tool for monitoring chemically selected plants combined with NIR spectroscopy. The method is cheap, fast, environmentally friendly, and can be used for field research.

Quality by design driven development and evaluation of thermosensitive hydrogel loaded with IgY and LL37-SLNs to combat experimental periodontitis

Egg yolk immunoglobulin (IgY) and LL37, potent antibacterial substances, can fight against periodontitis. This work aimed to develop a locally injectable hydrogel for potential co-delivery of special IgY and LL37-loaded solid lipid nanoparticles (LL37-SLNs) to synergistically inhibit the proliferation of oral pathogens, thus relieving periodontal inflammation and redness. The formulation of thermosensitive hydrogel loaded with IgY and LL37-SLNs was developed by adopting the Quality by Design approach. Then the formulations were optimized by two-factor three-level full factorial design by Design-Expert software. Finally, the optimized formulation was characterized and estimated in vitro and in vivo. In vitro release and antibacterial activity studies have revealed that the optimized formulation was homogeneous and can be released slowly, with sustainably antibacterial power. And the physical and chemical composition analysis and morphological observations further confirmed the sustained-release capability. On the other hand, in vivo studies proved that the optimized formulation significantly decreased gingival redness, bleeding, and plaque formation, avoided excessive resorption of alveolar bone, and reduced the levels of inflammatory factor in periodontitis rats. In conclusion, the optimized thermosensitive hydrogel loaded with IgY and LL37-SLNs may be a promising local sustained-release preparation for the effective treatment of periodontal diseases.

Formulation design and optimization of sustained release tablet dosage form of Diacerein

Diacerein is both an anti-inflammatory drug and an anti-arthritic agent. At present, no sustained release dosage form of Diacerein is commercially available. The aim of the study was to develop an optimized formulation of sustained release tablet dosage form of Diacerein that will be highly patient compliant. A three-level full factorial design was employed to develop nine different formulations of matrix tablets using a combination of Methocel K100M CR and Methocel K4M CR polymer at different ratios. The physical evaluation of both the drug-excipient powder mixtures and tablets of the nine formulations were performed and results were found to be within acceptable ranges for all tests. Dissolution profiles of tablets were obtained and the kinetics of drug release from the matrix tablets were determined by employing various mathematical models. The release data were simulated in Design Expert Software to carry out statistical analysis and the effect of both polymers on release patterns were observed after 1, 4 and 8 h in terms of contour plots and 3D surface plots, which were found to be statistically significant (p < 0.05). Finally, the overlay plot was obtained from which the optimized formulation of Diacerein was determined.

Experimental investigation into the evolution of microstructure and nanomechanical characterization of electric discharge deposited surface layers on Ti64

In the current study, Ti64′s surface has been transformed by electric discharge machining, and its microstructure, surface crack density, layer composition, and nanomechanical properties are examined, as well as the effects of peak current and pulse on time. The studies were carried out using a two-factor, three-level full factorial design. On the deposited layer, OM, XRD, EDS, SEM, and nanoindentation tests were performed. According to the variation in the process parameters, it was discovered that the deposited layer is not uniform and has a thickness range of 45–182 μm. Additionally, it has a dual microstructure that is both equiaxed and dendritic. Carbon deposition (up to 70% by weight), andcarbide formationwere all found to increase with parameter values. It is discovered that the layer's hardness is twice (718HV) as compared tothe basematerial. Additionally, a minor modification in reduced modulus has been noted. SCD and crack width were discovered to be rising as parameter level rose.

Multi-Criteria Optimisation of Friction Stir Welding Parameters for EN AW-2024-T3 Aluminium Alloy Joints.

The aim of this research was the selection of friction stir welding (FSW) parameters for joining stiffening elements (Z-stringers) to a thin-walled structure (skin) made of 1 mm-thick EN AW-2024 T3 aluminium alloy sheets. Overlapping sheets were friction stir welded with variable values of welding speed, pin length (plunge depth), and tool rotational speed. The experimental research was carried out based on a three-factor three-level full factorial Design of Experiments plan (DoE). The load capacity of the welded joints was determined in uniaxial tensile/pure shear tests. Based on the results of the load capacity of the joint and the dispersion of this parameter, multi-criteria optimisation was carried out to indicate the appropriate parameters of the linear FSW process. The optimal parameters of the FSW process were determined based on a regression equation assessed by the Fisher–Senecor test. The vast majority of articles reviewed concern the optimisation of welding parameters for only one selected output parameter (most often joint strength). The aim of multi-criteria optimisation was to determine the most favourable combination of parameters in terms of both the smallest dispersion and highest load capacity of the joints. It was found that an increase in welding speed at a given value of pin length caused a decrease in the load capacity of the joint, as well as a significant increase in the dispersion of the results. The use of the parameters obtained as a result of multi-criteria optimisation will allow a minimum load capacity of the joints of 5.38 kN to be obtained with much greater stability of the results.

Tolmetin Sodium Fast Dissolving Tablets for Rheumatoid Arthritis Treatment: Preparation and Optimization Using Box-Behnken Design and Response Surface Methodology.

Tolmetin sodium (TLM) is a non-steroidal anti-inflammatory drug (NSAIDs). TLM is used to treat inflammation, skeletal muscle injuries, and discomfort associated with bone disorders. Because of the delayed absorption from the gastro intestinal tract (GIT), the currently available TLM dosage forms have a rather protracted start to the effect, according to pharmacokinetic studies. The aim of this study was to create a combination for TLM fast dissolving tablets (TLM-FDT) that would boost the drug’s bioavailability by increasing pre-gastric absorption. The TLM-FDTs were developed using a Box-Behnken experimental design with varied doses of crospovidone (CP), croscarmellose sodium (CCS) as super-disintegrants, and camphor as a sublimating agent. In addition, the current study used response surface approach to explore the influence of various formulation and process factors on tablet qualities in order to verify an optimized TLM-FDTs formulation. The optimized TLM-FDTs formula was subsequently evaluated for its in vivo anti-inflammatory activity. TLM-FDTs have good friability, disintegration time, drug release, and wetting time, as well as fast disintegration and dissolution behavior. Significant increase in drug bioavailability and reliable anti-inflammatory efficacy were also observed, as evidenced by considerable reductions in paw thickness in rats following carrageenan-induced rat paw edema. For optimizing and analyzing the effect of super-disintegrants and sublimating agents in the TLM-FDTs formula, the three-factor, three-level full factorial design is a suitable tool. TLM-FDTs are a possible drug delivery system for enhancing TLM bioavailability and could be used to treat rheumatoid arthritis.

High-throughput volatile organic compounds removal in a sandwich-type honeycomb catalyst system combined with plasma

This paper reports on the ethyl acetate (EA) removal over a honeycomb catalyst using an experimental design methodology. A mathematical model based on the response surface methodology is developed to evaluate the effect of parameters such as specific input energy (SIE), humidity, and EA concentration on the response variables (i.e., removal and energy efficiency). A set of experiments based on a statistical three-level full-factorial design of the experimental method were performed to collect the removal data. The model (R12 = 0.9858 and R22 = 0.9305 for removal and energy efficiency, respectively) indicates a satisfactory correlation between the experimental and predicted values. The analysis results using the model show that SIE is the principal parameter affecting the EA removal. Besides, verification of the experimental results indicates that the EA removal of 90.93% and the energy efficiency of 2.91g.kWh−1 at 235 J L-1, 1.5%, and 30.3 ppm were achieved under optimal conditions.

Dually Cross-Linked Core-Shell Structure Nanohydrogel with Redox-Responsive Degradability for Intracellular Delivery.

A redox-responsive nanocarrier is a promising strategy for the intracellular drug release because it protects the payload, prevents its undesirable leakage during extracellular transport, and favors site-specific drug delivery. In this study, we developed a novel redox responsive core-shell structure nanohydrogel prepared by a water in oil nanoemulsion method using two biocompatible synthetic polymers: vinyl sulfonated poly(N-(2-hydroxypropyl) methacrylamide mono/dilactate)-polyethylene glycol-poly(N-(2-hydroxypropyl) methacrylamide mono/dilactate) triblock copolymer, and thiolated hyaluronic acid. The influence on the nanohydrogel particle size and distribution of formulation parameters was investigated by a three-level full factorial design to optimize the preparation conditions. The surface and core-shell morphology of the nanohydrogel were observed by scanning electron microscope, transmission electron microscopy, and further confirmed by Fourier transform infrared spectroscopy and Raman spectroscopy from the standpoint of chemical composition. The redox-responsive biodegradability of the nanohydrogel in reducing environments was determined using glutathione as reducing agent. A nanohydrogel with particle size around 250 nm and polydispersity index around 0.1 is characterized by a thermosensitive shell which jellifies at body temperature and crosslinks at the interface of a redox-responsive hyaluronic acid core via the Michael addition reaction. The nanohydrogel showed good encapsulation efficiency for model macromolecules of different molecular weight (93% for cytochrome C, 47% for horseradish peroxidase, and 90% for bovine serum albumin), capacity to retain the peroxidase-like enzymatic activity (around 90%) of cytochrome C and horseradish peroxidase, and specific redox-responsive release behavior. Additionally, the nanohydrogel exhibited excellent cytocompatibility and internalization efficiency into macrophages. Therefore, the developed core-shell structure nanohydrogel can be considered a promising tool for the potential intracellular delivery of different pharmaceutical applications, including for cancer therapy.

Micro-patterned graphite electrodes: An analysis and optimization of process parameters on hydrogen evolution in water electrolysis

The aim of this investigation is to enhance the hydrogen production rate using developed micro-patterned graphite electrode in water electrolysis method. Alkaline sodium hydroxide was used as an electrolyte for enhancing the hydrogen production through the continuous electrolysis process. The Box-Behnken design based four-factor three-level full factorial design was utilized to develop the regression model for the hydrogen production process. The response surface methodology was used to analyze the combined effects of voltage supplied, electrolyte molarity and electrode distance on hydrogen production rate. The maximum hydrogen production rate of 2.3 ml/min was achieved under the optimum conditions such as supply voltage of 2 V, electrolyte molarity of 0.3 M and an electrode distance of 10 cm. The predicted regression equation for the hydrogen production process showed positive effect with the experimental value. Also, the individual parameter on hydrogen yield has been evaluated using ANOVA analysis. The coefficient of determination (R2) of 0.98 showed that the model obtained was highly significant to predict the response accurately with below 2% error. The surface of the graphite microstructure considerably influenced the reaction of the electrolysis on hydrogen evolution rate. The micro-patterned electrode was compared with the planar electrode on the hydrogen production rate at optimum conditions and found a 60% increase in yield.

Analysis of faults in rotor-bearing system using three-level full factorial design and response surface methodology

This article presents the experimental and statistical methodology for localized fault analysis in the rotor-bearing system. These defects on outer race, on inner race, and on a combination of ball and outer race are considered. In this study speed, load and defects were considered as the essential process variables to understand their significance and effects on vibration response for the rotor-bearing system. Three factors at three levels were considered for experimentation, and the experiment was designed for L27 based on design of experiments (DOE) methodology. From the experiments, the vibration response results are recorded in terms of root mean square value for the analysis. Response surface methodology (RSM) is used for identifying the interaction effect of varying process parameters upon the response of vibrations by response surface plot. The rotor-bearing test setup is used for experimentation and is analyzed by using DOE. This study establishes the prediction of fault in the rotor-bearing system in combined parametric effect analysis and its influence with DOE and RSM.