Three-day Schedule Research Articles

Editors' Introduction

The contents of this special issue on women's film history have their starting point in the “Doing Women's Film and Television History II” international conference, which took place at the University of East Anglia, England, in April 2014. Several of the texts began life as keynote lectures and presentations, while others were inspired by the discussions that took place across its packed three-day schedule. The second conference of the Women's Film and Television History Network, UK-Ireland, the inaugural edition of which had taken place at the University of Sunderland in 2011,1 provided another opportunity for researchers, archivists, and activists to share their ideas around histories of women working in film and television, …

SENSATION AND SENSIBILITY AT THE KEYBOARD IN THE LATE EIGHTEENTH CENTURY: CELEBRATING THE TERCENTENARY OF C. P. E. BACH CORNELL UNIVERSITY, 2–4 OCTOBER 2014

This conference-festival at Cornell University was a highlight among the many events held worldwide in connection with C. P. E. Bach's tercentenary. In addition to an international line-up of visiting scholars who descended upon Ithaca (only then, it might be added, to ascend the formidable hill atop which Cornell is perched), the occasion drew together from within the university the Department of Music, the Westfield Center for Historical Keyboard Studies, the Division of Rare and Manuscript Collections and the Atkinson Forum in American Studies. The conference was conceived around Christopher Hogwood's appointment at Cornell as A. D. White Professor-at-Large. Hogwood had been expected to attend and preside over the conference as honorary chair, but in the wake of his death on 24 September 2014 the proceedings were instead dedicated to him. Performances of C. P. E. Bach's music were interwoven with paper sessions and other events throughout each day: in all, two keynote lectures, four paper sessions, four solo keyboard recitals, two vocal-instrumental concerts, a standing exhibition, a clavichord masterclass and even a glass harmonica demonstration filled out the whirlwind, three-day schedule.

Abstract B056: p70S6K activity drives local relapse in breast cancer

Abstract For early breast cancer (EBC) patients local relapse represents what mostly influences disease outcome. Notably, 90% of local recurrences occur at or close to the same quadrant of the primary cancer. Surgery itself and the consequent process of wound healing have been proposed to stimulate local recurrences via pathway(s) still to be clarified. Our previous studies implicated p70S6K pathway in breast cancer cell response to post-surgical inflammation, supporting the hypothesis that it may be crucial also for breast cancer recurrence. We designed an in vivo experimental model resembling the course of human BC, in which MDA-MB-231 breast cancer cells were bilaterally injected in nude mice mammary fat pads and, when primary tumors were grown, masses were surgically removed under anesthesia. After recovering, mice were followed up to detect appearance of local relapse. Using this model, we dissected the role of p70S6K during breast cancer growth and recurrence by modulating its activity using both genetic and pharmacologic approaches. Our results showed that p70S6K positively contributed to proliferation and survival programs in breast cancer cell lines. In vivo, the analysis of the impact of p70S6K on primary tumor growth highlighted that a robust p70S6K signaling was required for the initiation of the tumor masses. But, more importantly, interfering with p70S6K activity strongly impaired local relapse. A three-day schedule of peri-operative treatment using specific pharmacological inhibition of p70S6K1 was sufficient to reduce by 83% the rate of local recurrence. We showed that inhibition of p70S6K activity induced apoptosis in cells challenged to survive in a hostile environment, supporting the idea that p70S6K signaling is necessary for the survival of isolated cancer cells in breast microenvironment, following surgery. The significance of our results was confirmed in human EBC specimens, proving that p70S6K activity is robustly induced by surgery, also in human patients. Taken together, our results provide a biological rationale for peri-operative treatment targeting p70S6K pathway, directed to compensating the harmful consequences of surgery and to restraining local recurrence in early breast cancer patients. Citation Format: Ilenia Segatto, Stefania Berton, Maura Sonego, Samuele Massarut, Tiziana Perin, Gustavo Baldassarre, Barbara Belletti. p70S6K activity drives local relapse in breast cancer. [abstract]. In: Proceedings of the AACR Special Conference on Advances in Breast Cancer Research: Genetics, Biology, and Clinical Applications; Oct 3-6, 2013; San Diego, CA. Philadelphia (PA): AACR; Mol Cancer Res 2013;11(10 Suppl):Abstract nr B056.

Positive Effect of Nitric Oxide on Morphine-Induced Place Conditioning in Wistar Rats Treated by Colchicine Intra-Hippocampal CA1

Colchicine is a potent alkaloid neurotoxin known as a selective neurotoxin of granular cells of hippocampal formation. This research aimed to assess the consequences of hippocampal reward circuitry damaged by colchicine. Male Wistar rats were examined for saline conditioning a week after receiving of colchicine (1-8μg/rat, intra-CA1). Control group was simply microinjected saline (1μl/rat, intra-CA1). In addition, two categories of animals pre-treated by colchicine (2,8μg/rat, intra-CA1) were evaluated for place preference induced of morphine (2.5-7.5 mg/kg, s.c.) using a three-day schedule of unbiased paradigm. Also sham operated legend “normal” group passed the conditioning by morphine. This group was pre-treated saline (1μl/rat, intra-CA1) instead of colchicine. According to the results, the injection of colchicine (1-8μg/rat, intra-CA1) induced a place aversion in saline conditioning at the higher doses (8μg/rat). Furthermore, the colchicine treated rats showed histologically a significant ( p<0.001 ) decrease in numbers of pyramidal cells of CA1. On the other hand, morphine induced a significant place preference in animals pre-treated by colchicine (2,8μg/rat, intra-CA1). Moreover, pre-microinjection of L-arginine (0.3- 3μg/rat, intra-CA1), a precursor of nitric oxide (NO), pre-testing of morphine response caused a positive effect on expression of response to morphine. However, this effect was reversed when L-NAME (0.3-3μg/rat, intra-CA1) was injected into the CA1 prior to the administration of L-arginine (3μg/rat, intra-CA1). Based on data the neurotoxin, colchicine, may affect the place conditioning reward circuitry via decreasing of pyramidal cells in the CA1 area ( p<0.01 ), an effect which most likely can be improved by morphine administration peripherally. In conclusion the molecule NO into CA1 may have a positive force on morphine-induced place conditioning in Wistar rats received colchicine intra-CA1.

Treatment of anthracycline extravasation with dexrazoxane: Pharmacokinetics and update on efficacy and safety from three multicenter trials

2555 Background: Dexrazoxane prevented tissue necrosis in 98% of patients (pts) with biopsy proven anthracycline extravasation (AEV) in two international multicenter studies, TT01 and TT02 (Mouridsen HT et al Ann Onc Advance Access Dec 21, 2006). However, the pharmacokinetics (PK) of IV dexrazoxane in the three-day schedule is not established. Study TT04 was initiated to investigate PK. Patients and methods: TT04 is a prospective, open-label, single-arm, multicenter studies in pts with AEV. Consecutive pts with AEV are treated with a three-day schedule of IV dexrazoxane (1,000, 1,000, and 500 mg/m2) starting within 6 hr of AEV. Primary objective: Establish PK of IV dexrazoxane in the three-day schedule. Secondary objectives: obtaining additional efficacy and safety data. Plasma samples at 0, 1, 2, 4 and 24 hr day 1–3 are analyzed by HPLC-MS. PK parameters were calculated by a non compartmental method. TT01 and TT02 were open-label, single-arm, multicenter studies enrolling pts with biopsy proven AEV from 24 EU centers. Primary objective was to avoid tissue necrosis leading to surgery. Secondary objectives were to minimize delay of planned chemotherapy, reduce hospitalization, and avoid long term sequelae. Results: Six pts have entered the PK study. The average elimination was T½ ± SD of 127 ± 23, 144 ± 21, and 107 ± 29 min, day 1, 2 and 3, respectively. Pre-dose concentrations day 2 and 3 are = LOQ. Average AUC 0-t ± SD are 193 ± 93 (t= 24 hr), 196 ± 101 (t= 24 hr), and 46 ± 24 μg hr/ml (t= 4 hr), on day 1, 2 and 3, respectively. Cumulative data from the 3 studies: Surgery was avoided in 59/60 pts (98.3%). No delay in planned chemotherapy in 71%. 41% were hospitalized (median 3 days) due to the EV or its treatment. Mild pain (19%) and mild sensory disturbances (17%) were the most frequent sequelae: Reversible CTC grade 3–4 leucopenia/neutropenia in 45%, thrombocytopenia in 21% of the pts. Conclusion: There was no accumulation of dexrazoxane during the three-day schedule. Dexrazoxane was well tolerated and highly effective against anthracycline extravasation. Updated results will be presented. No significant financial relationships to disclose.

Business Propaganda in the Schools: Labor's Struggle against the Americans for the Competitive Enterprise System, 1949-1954

In early March 1953, the Americans for the Competitive Enterprise Sys? tem (ACES), a private, business-backed economic education association, opened up shop in Reading, Pennsylvania. Founded three years earlier in Philadelphia, ACES sought to demonstrate the superiority ofthe Ameri? can competitive system over any forms of To achieve that goal, the organization distributed literature, created a speakers' bureau, and offered educational activities for clergy, women's groups, and industrial workers. ACES' most extensive program, however, targeted high school students. It consisted ofa three-day schedule of activities that included class? room instruction in economics and tours of local industries. In 1953, after having successfully established a program in the Philadelphia public and parochial schools, ACES sought to expand its base of operations through? out Pennsylvania with chapters in Harrisburg, Lancaster, Erie, and Read? ing. Although unions throughout the state were suspicious ofthe organization from its inception, ACES met the strongest resistance in Reading. In that city, local socialists and trade unionists immediately mobilized along class lines and blocked ACES from gaining access to the Reading public school classrooms.1 During the decade after World War II, ACES was but one ofa num? ber of business groups engaged in an effort to sell Americans on the merits of the free enterprise system and the dangers of collectivism. Worried about the increasing power of the labor movement and about the public's apparent attraction to measures guaranteeing security for the working class through increased government control over the economy, the business com-

Phase I trial of a three-day schedule of cisplatin plus topotecan

Phase I trial of a three-day schedule of cisplatin plus topotecan

Randomized trial of carboplatin (CBDCA) and etoposide (E) administered either IV in a three-day schedule or in a protracted oral mode in non-small cell lung cancer (NSCLC): R Rosell, N Ribelles, A Abad, P Santabarbara ∗, A Barnadas, V Solano, A Font, F Molina. University Hospital Germans Trias i Pujol, Badalona, Barcelona and ∗ Bristol-Myers, Madrid.Spain

Randomized trial of carboplatin (CBDCA) and etoposide (E) administered either IV in a three-day schedule or in a protracted oral mode in non-small cell lung cancer (NSCLC): R Rosell, N Ribelles, A Abad, P Santabarbara ∗, A Barnadas, V Solano, A Font, F Molina. University Hospital Germans Trias i Pujol, Badalona, Barcelona and ∗ Bristol-Myers, Madrid.Spain

Mitoxantrone in relapsed or refractory acute nonlymphocytic leukemia.

Seventeen patients with relapsed or refractory acute nonlymphocytic leukemia were treated with 14 mg/m2 of mitoxantrone given in a 30-minute infusion daily for three days. If the day fourteen bone marrow showed residual leukemia, a second course was given at the same dose for two days. Eight patients (47%) entered complete remission. Three patients (17%) had a partial response, four (24%) did not respond, and two (12%) died with hypoplastic marrows during treatment. Seven of the 12 relapsed patients entered a complete remission, as did one of the five refractory patients. Toxicity was acceptable; prolonged myelosuppression, moderate hepatic toxicity, and stomatitis were the only problems. Several dose schedules of mitoxantrone have been studied by other investigators with varying results. The three-day schedule in the present study is similar to the schedule used for common induction regimens employing anthracycline drugs. On the basis of its activity and acceptable toxicity in relapsed and refractory ANLL patients, we feel that this schedule could be safely combined with other agents in future studies.