Bovine tuberculosis (BTB) still represents a significant public health concern and economic issue for livestockbreeders in Egypt. This research investigates the incidence of bovine tuberculosis in cattle slaughtered at the centralabattoir in Aswan, Egypt, in 2023. A total of 720 cattle were checked antemortem and postmortem at theabattoir. The suspected lesions were analyzed with acid-fast staining, microscopy, histology, ELISA, and RT-PCR.Based on gross tubercle lesions, the overall occurrence of bovine tuberculosis in cattle slaughtered at Aswan abattoirswas 3.2% (23/720), with thoracic lymph nodes and lungs exhibiting the highest frequency of tubercle lesionscompared to other organs. According to employed diagnostic tests, the prevalence of tuberculosis was estimatedas 68.6, 61, 56.5, and 78.3% for microscopy, histopathology, ELISA, and RT-PCR, respectively. These findingshighlight the incidence of bovine tuberculosis and the dissemination of tuberculous lesions among cattle slaughteredat Aswan abattoirs. Furthermore, such prevalence emphasizes the urgent need for a practical disease controlapproach and an organized surveillance system in the cattle population. One Health strategy is strongly advised tomitigate the zoonotic transmission to people and economic losses in the cattle industry.