Third Of Teeth Research Articles

Long-term prognosis for teeth with external cervical resorption based on periapical images and cone beam CT: A clinical study.

To evaluate whether supplemental information from CBCT changed long-term prognosis for teeth with external cervical resorption (ECR) compared to periapical (PA) images. Furthermore, to assess predictive findings in PA images and evaluate which findings in CBCT affected the long-term prognosis of teeth with ECR. One hundred and ninty-four patients, mean age 41.2, range 13-81, having 244 teeth with ECR were included. An initial long-term prognosis determined either good or poor was established based on intraoral images. Afterwards, the patients underwent CBCT, and final long-term prognosis was decided. From the PA images and CBCT, ECR using Heithersay's classification system, pulp involvement and extension of ECR was assessed. In CBCT, the number of surface lesion(s) was additionally assessed. Descriptive statistics evaluated changes in long-term prognosis after CBCT. Logistic regression analyses tested if findings in PA images and CBCT affected the long-term prognosis. Based on CBCT, out of 244 teeth the long-term prognosis was assessed to be poor for 173 (70.9%) teeth and good for 71 (29.1%) teeth. The long-term prognosis changed in 76 (31.1%) teeth after CBCT; 5 (2.0%) changed from poor to good, and 71 (29%) changed from good to poor long-term prognosis. In 81 (33.2%) teeth the H-class increased, and in 10 (4.1%) teeth the H-class decreased after assessing CBCT. In 70 (28.7%) teeth, there was a change from no pulp involvement to involvement of the pulp after CBCT; eight (3.3%) teeth changed in the opposite direction. H-class 2 and 4 in PA images significantly increased the probability for a change in long-term prognosis compared to an H-class 3 (p < .05). H-class 4, pulp involvement, ECR in the oral 1/3 of the root, and more than two surface lesions seen in CBCT significantly influenced a poor long-term prognosis (p < .05). Supplemental information from CBCT changed long-term prognosis in almost one third of teeth with ECR. In most cases, the long-term prognosis changed from good to poor. H-class 3 in PA images had a significant influence on change in long-term prognosis. Several findings in CBCT influenced a poor long-term prognosis.

Conservative Approach of Fractured Tooth with 7-Years of Clinical and Radiographical Follow-Up: Case Report

Clinical and radiographical diagnosis is crucial to treat a tooth with transverse root fracture (TRF) due to dental trauma (DT). This paper aimed to report a clinical case of TRF at the cervical third of tooth 21 injured by recurrent DT with 7 years of follow-up. A 7-years old female patient was referred to dental care after the first episode of DT in 2014. Base d on the radiographical examination, splinting of teeth 11 and 21 was performed using resin composite. After 11 months, the splint was removed and the patient was discharged. In 2015, the patient suffered a second DT of the same tooth. The clinical approach was splinting the traumatized tooth using orthodontic wire and resin composite. In 2018, a third episode of DT occurred. Then, the dental staff chose to maintain the splint and evaluate the case in a long-term. In 2021, based on bone tissue condition, dental fragment stability and radiographical follow-up, the splint was removed. The patient remains under regular follow up evaluations. It can be concluded that a conservative approach in cases of TRF, when possible, can preserve biological tissues.

Intentional Replantation – The novel technique for the management of separated instrument in apical third of tooth: A case report

Intentional replantation is a procedure in which an intentional tooth extraction is performed followed by reinsertion of the extracted tooth into its own alveolus. In this article, intentional replantation is described and discussed as a treatment approach for failed root canal treatment with broken instrument periapically in mandibular second molar.

Reinforcing the cervical dentin with bonded materials to improve fracture resistance of endodontically treated roots.

Introduction:Endodontic procedure leads to the loss of tooth structure resulting in fractures. Intraorifice barriers of bonded restorative materials placed in the cervical third of tooth may help in increasing fracture resistance.Materials and Methods:Human mandibular premolars (n = 75) underwent decoronation to adjust working length at 14 mm and prepared up to F3. They were obturated using gutta-percha and resin sealer AH-Plus and randomly divided into five groups (n = 15), Group 1: Control obturated with gutta-percha only. Groups 2, 3, 4, and 5 had placement of intraorifice barriers after the removal of 3 mm coronal gutta-percha such that Group 2: RMGI, Group 3: Self-adhering flowable composite, Group 4: Bulkfill Flowable Composite, and Group 5: mineral trioxide aggregate (MTA). Mounting of specimens was done in acrylic resin to expose coronal 3 mm and tested using the universal testing machine.Results:Group 1 (control) showed least fracture strength among all groups. Among those with intraorifice barriers, Group 2 Resin-modified glass ionomer cement showed maximum fracture resistance followed by Group 4 (Bulkfill composite) and Group 5 (self-adhering flowable composite) and least by Group 5 (MTA).Conclusion:The type of intraorifice barrier had a significant impact on root fracture resistance.

Effectiveness of various endodontic systems in treating teeth with C-shaped root canals

Introduction — C-shaped configuration is amongst the most complicated root canal varieties requiring additional effort for successful endodontic treatment. The goal of this study was the laboratory comparison of the endodontic treatment efficiencies via XP-endo vs. Gentlefile systems for the teeth with C-shaped root canals. Material and Methods — In laboratory settings, 36 teeth with established C-shaped configuration, extracted for medical reasons (24 mandibular premolars and 12 mandibular second molars), underwent the root canal treatment by XP-endo (XP) and Gentlefile (GF) systems. Treatment quality was evaluated on the basis of dye penetration tests and examination of root dentinal surface with a scanning electron microscope. The significance of differences between the groups was assessed via Mann-Whitney U test. Results — For GF instruments, the average size of stained area in the apical third of teeth was 3.33±2.73%, while in the coronal third it amounted to 13.49±13.58%. The results of using the XP instruments demonstrated the average size of stained area in the apical third equal to 15.45±12.48%, while in the coronal third it was 37.30±14.06%. The scanning electron microscopy revealed a rougher surface of dentin and the remnants of stained layer in root canals treated with GF system. Conclusion — Thus, XP instruments exhibited a better treatment quality compared with GF system. The obtained data may justify the choice of instruments for endodontic teeth treatment in case of an intricate root canal system.

Shrinkage Strains in the Dentin of Endodontically Treated Teeth with Water Loss

IntroductionDehydration has been considered as a potential contributor to vertical root fractures (VRFs) after root canal treatment (RCT). A loss of water could cause embrittlement of dentin and detrimental shrinkage strains. Senior patients have the highest risk of VRF. In this study, we characterized the spatial distribution in shrinkage of tooth roots with respect to donor age and prior RCT. MethodsSingle-rooted human teeth with and without prior RCT were collected from young (age <25 years) and old (age >60 years) adults. Transverse slices were sectioned from the apical, middle, and coronal thirds of the roots, and digital image correlation was used to evaluate shrinkage during free convection. Crack initiation and growth analysis was performed via optical microscopy, and bound water in dentin was characterized by Raman spectroscopy. ResultsThe rate of shrinkage was significantly higher (p ≤ .05) in the apical third than in the middle and coronal thirds of all teeth regardless of donor age. The highest shrinkage strain occurred in the apical third of old donor teeth with prior RCT. In addition, the RCT-treated old teeth suffered the highest percentage of water loss with dehydration. Cracks initiated from the root surface and extended toward the canal with loss of water and shrinkage. ConclusionsThe apical third undergoes significantly larger shrinkage strains with dehydration than the remainder of the root. Prior RCT exacerbates the extent of shrinkage, particularly in the teeth of seniors and after clinical function, which could increase the propensity for VRF.

Herausforderungen und Lösungsansätze bei der Diagnostik einer Wurzelquerfraktur im Röntgenbild

ObjectivesDiscussion of the importance of X-ray diagnostics in dental traumatology and requirement of multiple X-ray images in different axes for sufficient diagnostics.Clinical procedureThe 11-year-old patient showed up in the emergency unit of the dental clinic in Erlangen in April 2018 around 9 pm, approximately 2 hours after crashing in a slide in a public swimming pool. After detailed examination of the tooth 11 a root-crown-fracture with involvement of the pulp was diagnosed. The fragments of the tooth 11 were partially present but could not be joint together in order to restore the crown. The X-ray taken in occlusal view before emergency treatment did initially not show any abnormalities or fractures. No movement of the crown could be found, the tooth was tender to percussion and did not react to testing of sensibility. Emergency treatment included documentation via photographs and detailed information of the treatment steps: local anaesthesia was followed by a micropulpotomy using calcium hydroxide. Further dentine defects were capped using composite. The patient showed up in the dental clinic the following day complaining about slight pain (regio 11). The tooth was still tender to percussion and not reacting to testing of sensibility. An orthopantomogram for further examination of the temporomandibular joint showed a root fracture in the apical third of tooth 11. This diagnose was confirmed via another small X-ray using a different angle vertical to the tooth axis. The patient and her mother were informed about further treatment options (root canal treatment of the coronal part of the root, MTA plug) and the questionable outcome of tooth 11.Conclusions for practiceIn order to perfectly diagnose or exclude root fractures after dental trauma X-rays using different axes should be taken. Also, a high resolution cone beam computed tomography (CBCT) can be considered for further examination in complex cases.

Efficiency of XP endo Shaper (XPS) and irrigation protocol on the quality of cleaning the apical third of root canal: SEM study

Background/Aim: The aim of this study was to evaluate the efficacy of new rotary NiTi instrument XP-endo SHAPER (XPS) used with two irrigation protocols on the root canal cleaning in the apical area. Material and Methods: The research was conducted on 30 single-rooted teeth extracted for orthodontic reasons which were divided into the two groups. Instrumentation of the canals was conducted with XPS instrument and 2% solution of NaOCl was used as irrigant. Instrumentation in the first group was performed using a conventional continuous irrigation, in the second group, protocol of final irrigation was performed intermittently in 3 cycles. The SEM analysis of the apical third of the canal was performed on longitudinal root cross-section standardized photomicrography with a magnification of 2000X. Results: Results showed that a thicker smear layer was observed in the first group and with continuous irrigation protocol (2,10) in relation to the intermittent irrigation protocol in 3 cycles (1,96), but without significant differences. The walls of the root canal in the apical third of the samples of the second group were slightly cleaner (73.3%) in comparison with the teeth of the first group (64, 7%), but also without significant differences. Conclusions: The use of XPS and 2% solution of NaOCl in the root canal enables efficient cleaning of the apical third of tooth. The final irrigation protocol in three cycles improves the efficiency of the smear layer removal in the apical segment of the canal.

Use of a shade guide to test the perception and prevalence of natural tooth colour in people of Nepal

Background: Recent advances in colour matching have been driven by the market demand for high-quality esthetic restorations. Improved shade guides, availability of shade-taking devices and research in the area of human colour vision have improved the potential of clinicians to achieve excellent colour matched restorations. A thorough understanding of appearance attributes of natural teeth is required along with these new tools to maximize shade matching results.&#x0D; Aims and Objectives: The aim of the present investigation is to determine tooth shade among the people of Nepal.&#x0D; Materials and Methods: In the present study, shade of cervical, middle and incisal third of the facial surface of natural maxillary central incisor was measured with digital colorimeter, VITA Easy shade Advance 4.0. It is an optical reading device that can assess the wavelengths reflected back to its sensors. Lighting in operatory does not affect the shade measurement. It is extremely accurate. The limitation of study was its small sample size.&#x0D; Result: The shade obtained in the cervical third of tooth is 1M2 (37.33%), in the middle 1M1 (32.88%), and in the incisal third 2M1 (37.33%). Value 1 indicates lighter tooth colour and colour saturation is up to 2.&#x0D; Conclusion: It was concluded that the most prevalent shade in cervical third was 1M2, middle third was 1M1 and incisal third was 2M1.

Radiographic Evaluation of Periodontal Tissue Detected in Cone Beam CT Images for Teeth with and Without Apical Periodontitis A Retrospective Study

Aim of the study: The aim of the present work was to evaluate the alveolar bone level and periodontal ligament space thickness of teeth that have apical periodontitis and compare it with teeth without apical periodontitis using cone beam CT images.Materials & Methods: The study design was a retrospective cross sectional epidemiological study for evaluation of periodontal tissue of 100 teeth using cone beam CT images. The sample consisted of CBCT images of the maxillofacial region from database of 50 patients which were selected from Riyadh Colleges of Dentistry and Pharmacy. The 100 CBCT images were analyzed for the following variables 1-Periodontal ligament space of buccal surfaces at 3 sites cervical, middle and apical third. 2- Periodontal ligament space of lingual surfaces at 3 sites cervical, middle and apical third. 3-Alveolar bone level: the distance from CEJ to the crest of alveolar bone mesially and distally. These variables were detected on teeth with periapical periodontitis and compared with same teeth in the contralateral side of the same arch.Results: The buccal and lingual periodontal ligament space thickness in cervical, middle, and apical third of teeth with periapical lesion was statistically significant higher than the contra lateral teeth without periapical lesion of the same arch (p<0.05). Furthermore, the alveolar bone measures from CEJ to alveolar crest in both mesial and distal sides of the teeth with periapical lesion was statistically significant higher than the contralateral teeth without periapical lesion of the same arch (p<0.05).Conclusions: The present study showed that, periapical infection has negative effects on alveolar bone level and periodontal ligament space thickness. CBCT is an effective diagnostic method to visualize and measure the periodontal ligament space and alveolar bone level.

Sodium hypochlorite penetration into dentinal tubules after manual dynamic agitation and ultrasonic activation: a histochemical evaluation.

The aim of this study was to compare the effects of Manual Dynamic Agitation and Passive Ultrasonic Irrigation on sodium hypochlorite (NaOCl) penetration into dentinal tubules using its bleaching ability. Thirty-four single-rooted teeth with round-shaped root canals were distributed in two homogeneous groups and one control group, characterized by different NaOCl activation systems: Manual Dynamic Agitation and Passive Ultrasonic Irrigation. After instrumentation, all root canals were stained with 10% copper sulphate solution followed by 1% rubeanic acid alcohol solution under vacuum. Final irrigation was performed with 5mL of 5.25% NaOCl solution for 1min and activated with Manual Dynamic Agitation or Passive Ultrasonic Irrigation for another 1min depending on the treatment group. The teeth were transversely sectioned at the middle portion of the apical, middle, and coronal thirds and observed under light microscope. NaOCl solution penetration was evaluated by measuring the percentage of bleached circumference of the root canal relative to the stained circumference, bleached areas, mean, and maximum penetration depth. No differences in the evaluated parameters were observed between groups (p > 0.05). Within groups, an increase of values was recorded from apical to coronal direction as for percentage of staining, percentage of bleaching and bleached area. NaOCl penetration into dentinal tubules did not significantly vary among the three levels. No significant differences in penetration of sodium hypochlorite into dentinal tubules when activated by means of Manual Dynamic Agitation or Passive Ultrasonic Irrigation were observed in the apical, middle, and coronal thirds of teeth with single straight round root canals.

Distal root curvatures in mandibular molars: analysis using digital panoramic X-rays.

The aim of this study was to describe the degree of curvature in distal roots in the first and second permanent mandibular molars in a Chilean patient sample. A cross-sectional descriptive study was conducted in which digital panoramic X-rays were analysed. Examinations of patients under 18 years, with signs of distortion or alteration in the contrast or the presence of pathologies that affected visualisation of the roots and pulp-chamber floor of the teeth to be analysed were excluded. Using the AutoCad software, an angle was drawn to represent the curve of the root in its different thirds, drawing lines inside the root canal from the pulp-chamber floor to the dental apex. Using the classic definition of dilaceration (root curvature > 90°), its prevalence was established. 412 teeth and roots were analysed, finding a dilaceration prevalence of 0.73% (n = 3). 84.72% of the roots presented some type of curvature. The middle third had the highest percentage of curvatures and the greatest average of angular curvature, whereas the cervical third was the straightest. No significant differences were found between the degree of curvature and the gender of the subjects, except for the apical third of tooth 3.6. The analysis of curvature by root third offers to the clinician a better perspective of the directional change of the roots and does not limit it to just the presence of curves in the apical third. The report of the angular degree of the curvatures, in addition to the prevalence of dilacerations, informs to the clinicians about the likelihood of finding difficulties when treating root canals. (Folia Morphol 2018; 77, 1: 131-137).

Influence of the Calcium Hydroxide Intracanal Dressing on Dentinal Tubule Penetration of Two Root Canal Sealers.

Objective:The aim of this study was to evaluate the effect of a calcium hydroxide (CH) dressing on the tubular penetration of two endodontic sealers, AH Plus (Dentsply Maillefer, Ballaigues, Switzerland) and MTA Fillapex (Angelus, Londrina, Brazil).Methods:Seventy-two mandibular premolars with a single root canal were prepared with ProFile.04 rotary instruments (Dentsply Maillefer) and divided into four groups. In two groups, an intracanal CH dressing was placed for 15 days. The obturations were performed with lateral condensation of gutta-percha in combination with one of the tested sealers. The roots were transversely sectioned at the apical and middle levels. The percentage of sealer penetration in the root canal walls and the percentage of impregnated dentin area in the transverse sections were obtained using confocal laser scanning microscopy. Statistical analysis was performed using one-way analysis of variance (ANOVA) and Games-Howell test.Results:The CH dressing reduced the mean value of tubular penetration in the middle third of teeth obturated with AH Plus (P<0.01), whereas no difference was observed at the apical sections for both sealers.Conclusion:The CH dressing did not interfere with the apical penetration of both tested sealers, however, decreased the tubular penetration in the middle third of the AH Plus root canal fillings. Overall, MTA Fillapex presented higher tubular penetration than AH Plus obturations.

Healing of transverse root fracture evaluated by using cone beam computed tomography: Case report with 2 years follow-up

A human dental trauma usually occurs in young patients involving an anterior dentition. A horizontal root fracture (HRF) is uncommon, being only 0.5–7.0% of all traumatic dental injuries. A 9-year-old Saudi boy was received trauma on his face causing HRF at both right (#11) and left (#21) maxillary central incisors. The apical third of tooth #11 was fractured, and #21 was lost. Clinical and radiographic examinations investigation were done, and proper management was carried out. The patient was asymptomatic at 2 years follow-up, and the cone beam computed tomography shows good healing of the two fragments that could be done with fibrous tissue.

Sodium hypochlorite solution penetration into human dentine: a histochemical evaluation.

The aim of this study was to evaluate exvivo the penetration depth of sodium hypochlorite solutions, with and without surfactants, into human dentine using a histochemical stain and the bleaching ability of NaOCl. Extracted maxillary central incisors were decoronated and their root canals were shaped to a size 30, .07 taper. Dentinal tubules were stained with 10% copper sulphate solution followed by a 1% rubeanic acid alcohol solution under vacuum. Specimens were irrigated with 5 mL of Niclor 5 (5.25% NaOCl solution) or 5 mL of Hypoclean (5.25% NaOCl solution + surfactants) according to the corresponding treatment group, for 2min. A transverse section was obtained in the middle portion of the apical, middle and coronal thirds of each tooth. Under microscopic examination, irrigant penetration was evaluated by measuring the following: the bleached circumference of the root canal with respect to the stained circumference; bleached areas, mean and maximum penetration depth. For statistical analysis, Pearson's chi-squared test and one-way anova were used. Only 30% of dentine tubules in the apical portions were stained; therefore, these were excluded from further comparisons. In the middle and coronal thirds, the average bleached circumference of the root canal was 80-99% of the stained circumference and the bleached area was 73757-135107μm2 . The average penetration depth ranged from 39 to 62μm and maximum penetration from 74 to 131μm. For all reported results, no differences within and between groups were observed (P=1). No differences in penetration of irrigants with and without surfactants into dentinal tubules were observed in the middle and coronal thirds of incisor teeth.

Comparison of Tooth Discoloration Induced by Calcium-Enriched Mixture and Mineral Trioxide Aggregate.

Introduction:The aim of this in vitro study was to evaluate the tooth discoloration induced by calcium-enriched mixture (CEM) cement and mineral trioxide aggregate (MTA). Methods and Materials:Forty five endodontically treated human maxillary central incisors were selected and divided into three groups (n=15) after removing the coronal 3 mm of the obturating materials. In the MTA group, white MTA plug was placed in pulp chamber and coronal zone of the root canal. In CEM cement group, CEM plug was placed in the tooth in the same manner. In both groups, a wet cotton pellet was placed in the access cavity and the teeth were temporarily sealed. After 24 h the teeth were restored with resin composite. In the negative control group the teeth were also restored with resin composite. The color change in the cervical third of teeth was measured with a colorimeter and was repeated 3 times for each specimen. The teeth were kept in artificial saliva for 6 months. After this period, the color change was measured again. Data were collected by Commission International de I'Eclairage's L*a*b color values, and corresponding ΔE values were calculated. The results were analyzed using the one-way ANOVA and post-hoc Tukey’s test with the significance level defined as 0.05. Results:There was no significant differences between CEM group and control group in mean discoloration. The mean tooth discoloration in MTA group was significantly greater than CEM and control groups (P<0.05). Conclusion:According to the result of the present study CEM cement did not induce tooth discoloration after six months. Therefore it can be used in vital pulp therapy of esthetically sensitive teeth.

Evaluation of three root canal filling techniques through digital radiograph

Endodontic obturation consists of root canal filling by antiseptic or inert materials that promote a three-dimensional sealing and stimulate the repair process without interfering with it. Different obturation techniques and materials have been proposed to meet this requirement. Objective: To compare the root canal filling promoted by lateral condensation technique, Tagger's hybrid technique and McSpadden technique by assessing the filling quality through digital radiograph. Material and methods: A total of 45 extracted single-rooted human teeth were used and randomly divided into three experimental groups. After instrumentation, the teeth were filled by lateral condensation (n=15), Hybrid Tagger (n=15), and McSpadden techniques (n=15). Then, digital radiographs were taken with projected increased 10 times, at mesial-distal and buccolingual directions. Results: Visually, few empty spaces were detected at the three root thirds of teeth filled by different techniques. However, statistical analysis (Kruskal-Wallis) found no differences among the different groups, neither among the different thirds nor between both incidences evaluated. Conclusion: It was concluded that the three obturation techniques exhibited similar behavior in relation to the sealing of the root canal through digital radiograph.

Keratocystic odontogenic tumour arising as a periapical lesion

To document a case of a keratocystic odontogenic tumour (KOT) involving the apical region in the maxilla mimicking a periapical lesion of endodontic origin. Benign and malignant tumours, including odontogenic lesions, can be erroneously diagnosed as periapical radiolucencies. KOTs mimicking periapical lesions of endodontic origin are uncommon, especially when the lesions involve the maxilla. This article describes a 55-year-old man with a well-delimited, oval-shaped, radiolucent lesion, occupying the middle and apical third of teeth 22 and 23. After 30days, the clinical and radiographic findings remained unchanged and the patient was referred for surgical removal of the lesion. Clinical, radiographic and histopathological features are also discussed and compared with current literature.

Root apical third and canal morphology of teeth with hypercementosis

ABSTRACTObjective: This study aims at studying the influence of hypercementosis over root and root canal morphology us-ing different methods of observation (clearing technique, radiography, stereomicroscopy and optical microscopy). Methods: 130 teeth were selected for morphological com-parative evaluation; all teeth were previously radiographed and stereomicroscopically evaluated. Out of these, 60 teeth with hypercementosis and 30 without it were selected for clearing technique evaluation. The analysis was based on aspects such as: type of hypercementosis; root canal num-ber and configuration; root surface and presence of apical foramen and apical deltas. The remaining 20 teeth with hy-percementosis were microscopically compared to 20 teeth with normal root formation by means of the Hematoxylin and Eosin (H.E.) staining technique, so as to study the ce-mentum deposition pattern and morphological aspects of the root canal. The evaluation was performed by two ex-aminers and submitted to Kappa agreement test. The data obtained was compared through non-parametric Kruskal-Wallis one-way analysis of variance test, and the Dunn test was applied for individual comparisons. Results: The root clearing examination showed higher frequency of club shaped hypercementosis (65%) followed by focal hyperce-mentosis (35%). Teeth with hypercementosis showed sig-nificant increase in the presence of apical deltas (53.3%). A higher frequency of root canal constrictions (55%), and changes in the original root canal path (46.6%) were also observed. Microscopic evaluation supports the influence of hypercementosis over the morphological characteristics of root apical third formation. Conclusions: These findings show the existence of a complex root canal anatomy at the apical third of teeth with hypercementosis, which may hinder root canal treatment.Keywords: Hypercementosis. Dental pulp cavity. Endodontics.

The Effects of Propolis on Discoloration of Teeth

Objective: Propolis is a resinous material produced by honeybees that has recently gained fame as an antimicrobial agent. This study sought to assess the effects of propolis as an intra-canal medicament on tooth discoloration. The effect of its application technique on the degree of discoloration was investigated as well.Methods: This experimental study was conducted on 40 intact anterior human teeth. After access cavity and canal preparation, the teeth were randomly divided into three groups. In group A, propolis was placed inside the canals and pulp chamber. In group B, propolis was used as an intracanal medicament. Group C was considered as the control group and saline solution was injected into the canals. Labial surfaces of all teeth were digitally photographed using a digital camera (Fujifilm at one day, one week, 2 weeks, one month and 2 months time points. Color of teeth was assessed and measured at incisal, middle, and cervical segments using the CIELab system and Photoshop software. Collected data at different time points were statistically analyzed.Results: The overall color change in the two groups of A and B was significantly different from the control group (P<0.001). Significant changes in color were also noticed in follow up sessions in groups A and B (P<0.001). At 2 months, the difference in overall color change between incisal, middle and cervical thirds of teeth in groups A and B was not statistically significant.Conclusion: Based on the obtained results, application of propolis as an intracanal medicament can lead to clinical color changes in tooth crown. Its application technique has no effect on the degree of discoloration.