Third Day Of Training Research Articles

A dual-process model for cognitive training

A key goal in cognitive training research is understanding whether cognitive training enhances general cognitive capacity or provides only task-specific improvements. Here, we developed a quantitative model for describing the temporal dynamics of these two processes. We analyzed data from 1300 children enrolled in an 8 week working memory training program that included 5 transfer test sessions. Factor analyses suggested two separate processes: an early task-specific improvement, accounting for 44% of the total increase, and a slower capacity improvement. A hidden Markov model was then applied to individual training data, revealing that the task-specific improvement plateaued on the third day of training on average. Thus, training is not only task specific or transferable but a combination of the two. The models provide methods for quantifying and separating these processes, which is crucial for studying the effects of cognitive training and relating these effects to neural correlates.

Panax Notoginseng Saponin Rg1 Can Effectively Improve the Cognitive Function of 5 × FAD Mice.

In order to investigate the effect of notoginsenoside Rg1 on cognitive function in 5∗FAD mice and the mechanism of Cx43 in improving cognitive function in mice, the methods 5∗FAD mice are selected as experimental animals and normal mice as healthy control. They were divided into three groups: healthy control group (n = 10), disease group (n = 10), and treatment group (n = 10, Panax notoginsenoside Rg 1150 mg/kg/d) for 2 months. Two months later, three groups of mice were subjected to classical water maze and Y-maze to test the cognitive ability of mice, and the correct response times and total response time were recorded. At the end of the cognitive function test, the mice were executed, and the brain tissues were taken. The expression of Cx43 protein and the changes of glial cells and neurons in the brain of the mice were analyzed at the cellular level. After treatment with Panax notoginseng saponin Rg 1150 mg/kg/d, it was found that the escape latency of mice in the treatment group was significantly lower than that in the disease group from the third day of training (P < 0.05); the time spent on the platform quadrant and the number of times crossing the platform in the treated mice were significantly increased, and the difference was statistically significant (P < 0.05); the expression of Cx43 protein in the brain of mice after treatment was significantly higher than that of mice in the disease group (P < 0.05). Conclusion. Panax notoginseng saponin Rg1 can effectively improve the cognitive function of 5∗FAD mice by increasing the secretion of Cx43 protein, thus increasing the reactivity of glial cells and neurons.

Effectiveness of a training on the recognition of subtle facial emotions in health and social workers.

This study analyzed the effectiveness of a training course on the recognition of subtle facial emotions in health and social workers, especially those who work in contact with difficult and aggressive patients in psychiatric wards and emergency departments. A total of 177 health and social workers were involved in the training course (138 females and 39 men, mean age was 53.3 years). The training was implemented in three different editions of the course (a three day course each) on the prevention and management of violence and the learning achieved has been evaluated in each edition. During the second and third day of training, 14 photographs from the Ekman SETT Test, representing the seven universal emotions (anger, fear, sadness, disgust, contempt, surprise, happiness), were administered to all participants. Participants selected the emotion demonstrated in each photograph on a response sheet. Differences in the answers between these two days were tested. The participants in the three editions showed a statistically significant improvement in the subtle facial emotion expressions recognition ability. Female health and social workers recognized significantly better facial emotion expressions than their colleagues (difference in the two days for the emotions= 0,205; p=0.006). Considering the professional qualifications, physicians have shown less ability in recognizing facial expression of anger, compared to nurses and other health and social workers (difference in the scores for anger of the third and the second day= 0.213; p=0.024). The study showed that the ability to recognize subtle facial expressions of emotions can be trained. The skills acquired during the training can be used to enhance communication with patients, especially with difficult and aggressive ones and their family members in emergency and psychiatric settings. The course on prevention and management of violence should possibly include training on the recognition of subtle facial emotions.

Consistent verbal labels promote odor category learning

Recent research shows that speakers of most languages find smells difficult to abstract and name. Can verbal labels enhance the human capacity to learn smell categories? Few studies have examined how verbal labeling might affect non-visual cognitive processes, and thus far very little is known about word-assisted odor category learning. To address these gaps, we tested whether different types of training change learning gains in odor categorization. After four intensive days of training to categorize odors that were co-presented with arbitrary verbal labels, people who learned odor categories with odor-label pairs that were more consistent were significantly more accurate than people with the same perceptual experience but who had odor-label pairs that were less consistent. Both groups' accuracy scores improved, but the learning curves differed. The context of consistent linguistic cuing supported an increase in correct responses from the third day of training. However, inconsistent linguistic cuing delayed the start of approximating to target odor categorization until after the fourth day. These results show that associations formed between odors and novel verbal labels facilitate the formation of odor categories. We interpret this as showing a causal link between language and olfactory perceptual processing in supporting categorization.

Portable model for vasectomy reversal training

ABSTRACTObjectives To validate an experimental non-animal model for training of vasectomy reversal.Materials and Methods The model consisted of two artificial vas deferens, made with silicon tubes, covered by a white resin, measuring 10 cm (length) and internal and external diameters of 0.5 and 1.5 mm, respectively. The holder of the ducts is made by a small box developed with polylactic acid, using a 3D print. The objective of the invention is to simulate the surgical field of vasovasostomy, when the vas deferens are isolated from other cord structures. For validation, it was verified the acquisition of microsurgical skills during its use, in a capacitation course with 5 urology residents from a Hospital of the region. Along the training sessions, it was analyzed the time (speed) of microsurgical sutures, and quantification of the performance using a checklist. Collected data were analyzed using de BioEstat®5.4 software.Results Medium time for the completion of microsurgical sutures improved considerably during the course, and reached a plateau after the third day of training (p=0.0365). In relation to the checklist, it was verified that during capacitation, there was significant improvement of the scores of each participant, that reached a plateau after the fourth day of training with the model (p=0.0035).Conclusion The developed model was able to allow the students that attended the course to gain skills in microsurgery, being considered appropriate for training vasectomy reversal.

Feasibility and acceptability of training community health workers in ear and hearing care in Malawi: a cluster randomised controlled trial

ObjectiveTo assess the feasibility and acceptability of training community health workers (CHWs) in ear and hearing care, and their ability to identify patients with ear and hearing disorders.DesignCluster randomised controlled...

Spatial memory formation differentially affects c-Fos expression in retrosplenial areas during place avoidance training in rats.

The retrosplenial cortex is involved in spatial memory function, but the contribution of its individual areas is not well known. To elucidate the involvement of retrosplenial cortical areas 29c and 30 in spatial memory, we analyzed the expression of c-Fos in these areas in the experimental group of rats that were trained in a spatial place avoidance task, i.e. to avoid shocks presented in an unmarked sector of a stable arena under light conditions. Control rats were trained in the same context as the experimental rats either without (Control-noUS) or with shocks (Control-US) that were delivered in a random, noncontingent manner for three days. On the first day of place avoidance learning, the experimental group exhibited c-Fos induction in area 29c, similar to both control groups. In area 30, similarly high levels of c-Fos expression were observed in the experimental and Control-US groups. On the third day of training, when the experimental group efficiently avoided c-Fos expression in areas 29c and 30 was lower compared with the first day of training. In area 29c c-Fos level was also lower in the experimental than in comparison to the Control-US group. In area 30, c-Fos expression in the experimental group was lower than in both control groups. In conclusion, areas 29c and 30 appear to be activated during spatial memory acquisition on the first day of training, whereas area 30 seems suppressed during long-term memory functioning on the third day of training when rats effectively avoid.

– Sports medicine Whole-body cryostimulation and oxidative stress in rowers: the preliminary results

IntroductionThe effect of whole-body cryostimulation (WBC) on the biomarkers of oxidative stress, lysosomal enzymes, creatine kinase and cortisol was studied.Material and methodsThe rowers underwent two 6-day training cycles: with pre-training daily WBC (temperature: from –125°C to –150°C) and without cryostimulation (control). Blood samples were taken before and after the third and sixth day of training.ResultsThe activity of superoxide dismutase and glutathione peroxidase was lower (by 44% and 42%, respectively) after the third day of training with WBC than without WBC. The concentration of lipid peroxidation products was also lower after the training preceded by WBC. Moreover, the acid phosphatase activity was 50% lower after the third day of training with WBC than training without WBC. Considering the antioxidant enzymes activity during training without WBC, the increase of superoxide dismutase and glutathione peroxidase activity was observed after the third day of training (by about 74% and 100%, respectively). The level of lipid peroxidation products also increased after the training without WBC. No statistically significant changes were observed in creatine kinase activity after the training preceded with WBC, while after the training without WBC activity of this enzyme was two-fold higher than before the training.ConclusionsThe use of WBC prior to training may reduce the risk of oxidative stress and the extent of muscle fibre injuries provoked by intense exercise. The WBC seems to be an effective and safe method for limiting exercise-induced damage; thus it may be used in biological regeneration of sportsmen.

The effect of apamin, a small conductance calcium activated potassium (SK) channel blocker, on a mouse model of neurofibromatosis 1

Neurofibromatosis 1 (NF1) is a common genetic disorder known to cause a variety of physiological symptoms such as the formation of both benign and malignant tumors, and is also known to cause visuospatial learning deficits. Mouse models of NF1 show increased GTP activation of ras which may alter K+ channels. One candidate K+ channel that may contribute to deficits in NF1 is the SK (small conductance calcium-activated potassium) channel due to its role in regulation of long term potentiation (LTP), a mechanism of learning which has been shown to be impaired in Nf1+/− mice. We found that administration of apamin (SK antagonist) either through i.p. injection or micro-osmotic pump to Nf1+/− mice significantly improved performance on the water maze task in comparison to saline treated Nf1+/− mice on the third day of training and on the corresponding probe test. In this study we demonstrate a possible mechanism for the learning deficits seen in Nf1+/− mice and a possible drug therapy for rescuing these deficits.

Protocolo de treinamento muscular inspiratório pré-operatório em cirurgia cardíaca eletiva: estudo piloto

Introdução: Indivíduos com fraqueza muscular respiratória no pré-operatório de cirurgia cardíaca possuem maior risco de desenvolver complicações pulmonares pós-operatórias. Objetivo: Avaliar um protocolo pré-operatório de treinamento muscular inspiratório para indivíduos que iriam submeter-se à revascularização do miocárdio. Métodos: Doze pacientes foram divididos em grupo controle e de treinamento muscular inspiratório. Utilizaram-se o Threshold®IMT, com carga de 30% da PImáx, reajustada a cada três dias, totalizando 15 dias de treinamento, e o teste de normalidade Shapiro-Wilk. As comparações de médias foram realizadas por meio do teste “t” de Student pareado e não pareado. Resultados: Constatou-se um ganho de 37cmH2O e de 33cmH2O para as pressões inspiratória e expiratória máximas, respectivamente, ao comparar os valores antes e após o treinamento, sendo significativo a partir do terceiro dia de treino. Conclusão: O protocolo testado neste estudo mostrou-se eficaz para aumentar a força muscular pré-operatória a partir do terceiro dia de treinamento domiciliar.

Association learning-dependent increases in acetylcholine release in the rat auditory cortex during auditory classical conditioning

The cholinergic system has been implicated in sensory cortical plasticity, learning and memory. This experiment determined the relationship between the acquisition of a Pavlovian conditioned approach response (CR) to an auditory conditioned stimulus (CS) and the release of acetylcholine (ACh) in the primary auditory cortex in rats. Samples of ACh were collected via microdialysis during behavioral training in either an auditory classical conditioning task or in a non-associative control task. The conditioning group received daily pairings of a white noise CS with a sucrose pellet unconditioned stimulus (US), while the control group received an equal number of CS and US presentations, but with these stimuli being presented randomly. Training was conducted on three consecutive days, with microdialysis samples being collected on Days 1 and 3 in separate sub-groups. The level of ACh released in the auditory cortex during conditioning trials increased from the first to the third day of training in the conditioning group as rats acquired the CR, but did not change in the control group, which did not acquire a CR. These data provide direct evidence for the hypothesis that ACh release increases in the primary auditory cortex during natural memory formation, where cholinergic activation is known to contribute to the formation of specific associative representational plasticity in conjunction with specific memory formation.

Epidural cortical stimulation enhances motor function after sensorimotor cortical infarcts in rats

This study examined whether epidurally delivered cortical electrical stimulation (CS) improves the efficacy of motor rehabilitative training and alters neuronal density and/or cell proliferation in perilesion cortex following ischemic sensorimotor cortex (SMC) lesions. Adult rats were pre-trained on a skilled reaching task and then received partial unilateral SMC lesions and implantation of electrodes over the remaining SMC. Ten to fourteen days later, rats received daily reach training concurrent with anodal or cathodal 100 Hz CS or no stimulation (NoCS) for 18 days. To label newly generated cells, bromodeoxyuridine (BrdU; 50 mg/kg) was administered every third day of training. Both anodal and cathodal CS robustly enhanced reaching performance compared to NoCS controls. Neuronal density in the perilesion cortex was significantly increased in the cathodal CS group compared to the NoCS group. There were no significant group differences in BrdU-labeled cell density in ipsilesional cortex. Staining with Fluoro-Jade-B indicated that neurons continue to degenerate near the infarct at the time when cortical stimulation and rehabilitation were initiated. These data indicate that epidurally delivered CS greatly improves the efficacy of rehabilitative reach training following SMC damage and raise the possibility that cathodal CS may influence neuronal survival in perilesion cortex.

Posttraining intrahippocampal infusion of a protein kinase AII inhibitor impairs spatial memory retention in rats

The role of protein kinase AII (PKA II) in spatial memory retention in male rats and its regulation of cholinergic gene expression were explored through the effects of intrahippocampal infusion of H-89, a selective PKA II inhibitor. Alterations in escape latency, travel distance, and swimming speed in a Morris water maze were measured. Animals were trained for 3 days; each day included two blocks, and each block contained four trials. Stereotaxic surgery was employed for the infusions after the last trial on the third day of training, and the animals were tested 48 hr after surgery. Bilateral intrahippocampal infusion of H-89 (2.5 or 5 microM) into the CA1 region generated significant alterations in escape latency and traveled distance but not swimming speed. The response was fairly dose dependent, and the maximal effect was obtained with 5 microM H-89. After behavioral testing, several of the infused animals were transcardially perfused and their brains removed. Brain tissue sections from these rats were subjected to immunohistochemical staining analysis with anticholine acetyltransferase (ChAT) antibodies. These analyses indicated that 5 microM H-89 infusions qualitatively reduced the density of ChAT-containing cholinergic nerve terminals in the dorsal hippocampus. The intrahippocampal infusions with 5 microM H-89 also caused an apparent reduction in the number of ChAT-containing neurons in the medial septum. Our results suggest that PKA II is involved in regulation of cholinergic gene expression and plays an important role in spatial memory retention in rats.

A qualitative aspect of learning-sensitive open field ambulation in mice.

Maze training was shown to affect qualitative aspects of the learning-sensitive open-field (OF) ambulation in male Swiss albino mice. The changes in thigmotactic (wall-seeking) behaviour were most pronounced when significant signs of learning occurred, usually on the third day of training. Also daily changes in thigmotaxis from before to after training were found. Mice not subjected to maze training exhibited a different thigmotactic pattern of behaviour. The present series of experiments indicated that separate starting-points (centre or wall) influenced the thigmotactic behaviour as opposed to the shape of the OF (circular or square).

The contribution of training to sensitivity in the nociceptive paw-withdrawal test

An important limitation in behavioral tests of the hyperalgesic and analgesic effects of drugs is the low sensitivity of the measuring technique associated with inter- and intra-subject variability. Since familiarity with the experimental situation and novelty of the stimulus significantly influences an animal's behavior, we have tested the hypothesis that intensive training of rats, in the Randall-Selitto paw-withdrawal test, will increase the sensitivity of this test for detecting hyperalgesic effects of intradermal injections of the inflammatory mediator, bradykinin (BK). Mean baseline of the mechanical nociceptive threshold decreased between the first and second, and stabilized by the third day of training. Although there was no significant difference between the effects of BK or its vehicle (saline) on nociceptive threshold on day 1, on subsequent days BK produced a dose-dependent decrease in nociceptive threshold that could be distinguished from the effect of vehicle. These data demonstrate that training in the experimental paradigm can increase sensitivity for detecting hyperalgesic effects of inflammatory mediators in behavioral tests.

The role of homecage environmental stimuli in the facilitation of shock-motivated spatial discrimination learning in rat pups.

In three experiments we examined the role of homecage environmental stimuli on learning an aversively motivated spatial discrimination task in 11-day-old rats. Varying the presence and absence of nest shavings in the correct and incorrect arms of a T-maze in Experiment 1 revealed that nest shavings had both nondirective facilitation and approach-eliciting properties. Training the 11-day-old rats in the presence or absence of shavings over three daily sessions and comparing their performance on the third day of training with that of a maturation control in Experiment 2 indicated that there is a residual training effect of the approach-eliciting property of nest shavings on shock-escape behavior. In Experiment 3, using nest shavings either as an irrelevant stimulus or as a redundant relevant cue in an aversively motivated T-maze reversal task suggested that 11-day-old rats acquire the discrimination task using non-nest spatial or directional cues as well as those provided by nest shavings. Overall, these results suggest that nest shavings introduced into aversively motivated tasks create a learning environment comparable to that of appetitively motivated instrumental tasks utilizing suckling from an anesthetized dam as a reinforcer.

Radial-maze performance and structural variation of the hippocampus in mice: a correlation with mossy fibre distribution

Twenty-four male mice, belonging to 8 different inbred strains, were tested in an 8-arm radial maze. Clear strain differences were found for performance on the third day of training, which correlated very strongly with the size of the hippocampal intra- and infrapyramidal mossy fibre (iip-MF) terminal fields. These results, combined with those from earlier experiments, indicate that genetic variations of the iip-MF projection influence processes that determine behavioural abilities of mice.

Acquisition of intracranial self-stimulation in medial prefrontal cortex of rats facilitated by amphetamine

Two groups of rats were trained to lever press for intracranial self-stimulation (ICSS) in the medial prefrontal cortex (mPFC) using a uniform amount of stimulation for all animals. One group acquired the lever pressing task very gradually during saline pretreatment but dramatically improved its rate of acquisition during the third week of training when pretreated with d-amphetamine (0.5 mg/kg). Administration of amphetamine to the other group of rats before each of the first five training sessions greatly facilitated acquisition of the ICSS task, and a significant improvement in performance over the saline control group appeared on the third day of training. After ICSS performance had stabilized, testing the animals revealed a significant amphetamine-induced increase in rate over the dose range of 0.25 to 1.0 mg/kg. These effects of amphetamine suggest that ICSS in mPFC is sensitive to changes in catecholamine neurotransmission during both the acquisition and maintenance of this behavior.

Unabh�ngigkeit der bewertung zweier musterparameter von deren unterschiedlichkeitsgrad bei der Dressur

Bees were trained on a black disc (rewarded) against a smaller grey disc (unrewarded). Thus the bees had the choice of distinguishing between the two training figures by relying on the difference in size or on the difference in greyness or on both parameters. The aim of the present investigation was whether the relative weight of these parameters depended upon the differences in size and greyness presented during the training phase. 1. During the first two days of training the relative rating of these parameters changed in some, though not all individuals. In these cases the size of the discs rose in importance while the greyness was rated uniformly high (Fig. 3). Later, from the third day of training onwards the parameters were rated constantly. 2. Whenever size and greyness of the two training figures differed noticeably the bee relied on both parameters. The bee rated both parameters independently of the quantity of the differences in size and greyness presented during the training (Fig. 5). Thus the bee's analysis of the tested patterns cannot be attributed solely to the training paradigm, but seems to rely also on given (innate) "analyzers" (cf. Sutherland and Mackintosh, 1971). The type of training patterns only determines which "analyzers" influence the choice reaction. 3. The bee's "analyzer for size" had an influence on the choice reaction only if there was a noticeable size-difference between the training figures. The "analyzer for shades of grey", however, strongly influenced the choice reactions also in those cases where the training figures showed no difference in greyness but only differences in size (Fig. 2).

Successive Negative Contrast Effects with Goldfish (Carassius Auratus)

Goldfish were trained with large (10 pellets of freeze-dried tubifex worms) or small (1 pellet) reward as the appetitive Us in a forward classical conditioning situation. Activity (Cr) was recorded during each 9.5-sec. Cs-Us interval. The fish experienced 5 days of training with 20 trials per day. One-quarter of the classically conditioned fish with a given Us magnitude were shifted to the opposite Us after the third day of training, and one-quarter were shifted in the middle of the fourth day of training. Fish that were downward-shifted (3 or 3.5 days of training) produced negative contrast effects; that is, they became significantly less active during Cs presentations than fish maintained on the small Us in both phases of the study. Downward shifts were not as rapid as downward shifts obtained with rats in instrumental learning situations. Upward-shifted fish (3 or 3.5 days of training) increased activity, but did not attain the activity level of fish maintained on the large Us in both phases of the study.