Thin Sections Research Articles

Yeast fungi in the Ediacaran stromatolites of the Siberian Platform

Yeast fungi are extremely diverse, very widespread, and have a long history of industrial use; however, the time of their origin and early ecology on Earth remain unclear. This work fills this gap in scientific research, and, for the first time, descriptions are given with the allocation of new taxa of discovered yeast fungi in the Ediacaran (635–538 Ma). They are perfectly preserved in the form of microfossils and participated in the development of silicified columnar stromatolites (reef-like organogenic formations). The rock material was collected from the Byuk Formation (about 550 Ma) in the Berezovsky trough in the east of the Siberian platform. It was studied in thin sections of stromatolites on a scanning electron microscope. Microfossils were studied using standard comparative morphological methods. Their biological interpretation and systematic identification were conducted using analogy, the most widespread and efficient method of scientific research. The fungal (yeast) nature of the microorganisms discovered is indicated by the following features: reproduction by budding, sporulation, and division of individuals (cells), and properties of cell growth and their ecology (parasitization on the green algae thalli). New taxa of fossil yeast fungi have been identified: genus Tungusia, type-species Tungisia mane. We suggest that these microorganisms were the early ancestors of the modern yeast fungi. Keywords: Tungusia, green algae, Ediacaran, Byuk Formation, Siberian platform.

Insights into the K–Pg extinction aftermath: The Danish Cerithium Limestone Member

The Cretaceous–Palaeogene (K–Pg) mass extinction about 66 Ma ago was one of Earth's largest mass extinction events. The demise of calcifiers, among others, influenced biogeochemical cycles and changed the conditions for carbonate deposition in the global ocean. This study addresses the sedimentology and carbonate microfacies of the Cerithium Limestone Member of the Rødvig Formation within the renowned Stevns Klint succession in Denmark. The limestone was deposited in the earliest Danian Stage, immediately after the K–Pg mass extinction. It is a pale yellow, partly cemented unit with a dense network of Thalassinoides burrows and numerous flint nodules. Studies of the thin sections revealed that the Cerithium Limestone Member is more variable than expected from its overall homogeneous appearance at the macroscopic scale. The thin sections and scanning electron microscope (SEM) images showed that the highly bioturbated limestone consists of four principal microfacies: a mudstone, a wackestone and two different packstones. The 30 to 120-cm thick Cerithium Limestone Member fills depressions between low-amplitude mounds in the Maastrichtian chalk. The lowermost part constitutes a thin layer of a bryozoan-rich packstone, probably reworked from the crests of the Maastrichtian mounds. The successive part of the member is dominated by wacke stone with mainly foraminifera (planktic and benthic), molluscs and echinoderm debris, and in some areas an abundance of peloids. The foraminifera- and mollusc rich packstone appears in lenses. The mudstone contains few foraminifera and is linked to burrows and syn-sedimentary fractures. SEM observations revealed that the Cerithium Limestone Member corresponds to a dispersed micrite, with small calcite crystals ~1–4 µm in size. The general shape of these calcite crystals suggests precipitation from cyanobacterial activity and, thus, a microbial genesis for the micrite of the Cerithium Limestone Member.

Pore-throat structure, fractal characteristics and permeability prediction of tight sandstone: the Yanchang Formation, Southeast Ordos Basin.

As a typical tight reservoir and an important site for unconventional hydrocarbon accumulation, the Chang 6 member of the Yanchang Formation is characterized by complex pore structures and strong heterogeneity. Analysing and characterizing the pore-throat structure is highly important for optimizing oil recovery processes. To clarify the nonhomogeneity and structural characteristics of the pore throats in the southeastern Ordos Basin, tight sandstone from the Chang 6 member was selected for analysis. Casting thin section (CTS), scanning electron microscopy (SEM), cathodoluminescence (CL), X-ray diffraction (XRD), and mercury intrusion capillary pressure (MICP) analyses were conducted. The results, revealed that intergranular pores, feldspar-dissolved pores, intergranular-dissolved pores, and microfractures were the predominant pore types found within the samples. By combining the results of MICP analysis with those of fractal theory, the pore-throat structure of each sample can be categorized into two types: large-scale and small-scale. Fractal theory was employed to characterize the complex and irregular pore-throat structure of the reservoir quantitatively. The average fractal dimension of large pores (D1) was 2.8094, whereas for small pores (D2), it was slightly lower than that of large pores, averaging 2.5325. These findings underscore that large-scale pore-throat structures are more complex and exhibit greater heterogeneity. Compared with those of large pores, the pore-throat structure parameters of small pores exhibit a more significant correlation with reservoir properties and fractal dimensions. Therefore, small pores are the primary contributors to the reservoir storage space and are key factors influencing the pore-throat structure of the Chang 6 tight sandstone. Based on the pore-throat radius and considering the influence of fractal characteristics on the pore structure, a nonlinear permeability prediction model was created using multiple regression analysis. Among these equations, the pore-throat radius corresponding to a mercury saturation of 40% (r40) emerged as the most effective predictor of permeability for tight sandstone. The investigation of micropore-throat structures in tight oil reservoirs holds practical significance for comprehending the distribution and accumulation of tight oil and guiding oil field development.

Detecting the presence of different Retzius periodicities at the population level from repetitive linear enamel hypoplasia among Lufengpithecus lufengensis and Pongo pygmaeus.

Reconstruction of life histories for fossil and living primates draws on rate of enamel layering, termed Retzius periodicity (RP in days) expressed as surface perikymata, during dental crown formation. Disclosure of RP through thin sectioning is destructive; consequently, sample sizes are inadequate to detect the range of RPs present in discrete taxa. We propose an additional method to detect RPs at the population level based on twice-yearly average recurrence of linear enamel hypoplasia (rLEH) in apes shown by prior studies. Casts of teeth from orangutans (Pongo pygmaeus) (n = 40) and Lufengpithecus lufengensis (n = 57) from Late Miocene Shihuiba, China, (133 and 138 LEH, respectively) were recorded with scanning electron microscope (SEM) and confocal microscopy to yield perikymata counts between episodes of LEH. Frequency distributions of aggregated perikymata counts between LEH were compared to frequency distribution of tooth-specific ratios of perikymata counts between successive LEH (this latter step removes effects of RP differences within a sample). Drawing on prior research, two successive intervals between LEH span 1 year on average. Ratios of successive to previous intervals between LEH show that orangutans and Lufengpithecus exhibit two asymmetric intervals centered on 5.3 and 6.7 months, likely reflecting the effect of axial tilt insolation on phenology. Estimated RPs are not unimodal but show a range from 7 to 12 in Lufenpithecus and 8 to 11 in Pongo, comparable to published values. Repetitive LEH is sufficiently regular to detect additional RPs which, in the case of Lufengpithecus, have yet to be demonstrated histologically.

Preparation and Sectioning of Paraffin-Embedded Tissue for Histology and Histochemistry.

Cutting thin sections of plant tissues enables observation of internal structures and inner cell layers, and it facilitates various histochemical staining methods. Paraffin embedding gives the tissues a uniform stiffness that is sufficient for sectioning with a microtome. Here, we describe the procedures for preparation of paraffin-embedded tissues and the techniques for sectioning with a microtome. This method is applicable to various types of tissues, including rice shoot apex and immature inflorescence.

Relationship between bone union and degree of bone marrow fibrosis at resection margins of advanced mandibular ORN

BackgroundThe pathological evaluation of cancellous bone at resection margins of mandibular osteoradionecrosis (ORN) has not been well elucidated. Here, we developed a unique classification system for evaluating the degree of bone marrow fibrosis, one of most common pathological features, in patients with mandibular ORN, based on which we investigated its relationship with treatment outcome.MethodsThis study included 15 patients who underwent mandibulectomy and free fibula osteocutaneous flap reconstruction. The extent of mandibulectomy was determined, with safety margins of approximately 10 mm from the apparent osteolytic areas on preoperative computed tomography image. Special staining was performed on thin sections from center of the osteolytic areas (medial area) and bilateral resection margins, and the degree of bone marrow fibrosis was evaluated and investigated its relationship with presence of bone union as a treatment outcome.ResultsThe degree of bone marrow fibrosis of medial area was significantly higher than those of resection margins. Although most resection margins had collagen fibers which indicate severe fibrosis, all transferred fibula flaps achieved bone union.ConclusionWhen mandibulectomy is performed with safety margins of approximately 10 mm from the apparent osteolytic areas, all transferred fibula flaps achieved bone union regardless of the degree of bone marrow fibrosis at resection margin. In other words, the association between severe bone marrow fibrosis at resection margins and treatment outcome was not seen.Clinical RelevanceSetting safety margins of approximately 10 mm may achieve bone union, but further study is needed.

Double-eigenvalue bifurcation and multistability in serpentine strips with tunable buckling behaviors

Serpentine structures, composed of straight and circular strips, have garnered significant attention as potential designs for flexible electronics due to their remarkable stretchability. When subjected to stretching, these serpentine strips buckle out of plane, and previous studies have identified two distinct buckling modes whose order of appearance may interchange in serpentine structures with a single cell. In this study, we employ anisotropic rod theory to model serpentine strips as a multi-segment boundary value problem (BVP), with continuity conditions enforced at the interface between the straight and curved strips. We solve the BVP using methods of continuation, and our results reveal that: (1) the exchange of the two buckling modes in a single-cell serpentine strip is induced by a double-eigenvalue and associated secondary bifurcations, which also alter the stability of the two buckling modes; (2) a variety of stable states with reversible symmetry can be manually obtained in tabletop models and are found to be disconnected from the planar branch in numerical continuation. Furthermore, we demonstrate that modulating the strip thickness across different cells leads to the initiation of buckling in the thinnest section, thereby allowing for the tuning of buckling modes in serpentine strips. In structures with two cells, the sequence of the two buckling modes can also be controlled by designing serpentine strips with nonuniform height. This work could enhance the mechanical design of serpentine-interconnect-based flexible structures and could have applications in multistable actuators and mechanical memory devices.

Precontouring as a Tool to Improve the Laser Powder Bed Fusion Printability of Inconel939 Thin Walls

This work investigates the influence of adding a contour scan prior to hatching (precontouring) on the printability of Inconel 939 thin‐wall sections produced by laser powder bed fusion. Walls with thicknesses ranging from 500 to 1500 μm, manufactured with two hatch scanning parameter sets that give rise to different microstructures and very high density in bulk samples of the same alloy, are investigated. It is shown that, while the texture as well as the grain size and shape is only influenced moderately by the contour scanning strategy, precontouring does lead to a significant reduction of the melt pool depth, an effect that is increasingly pronounced with decreasing wall thickness. This work highlights the role of the precontour scan as a heat sink, shifting the normalized enthalpy input toward lower values and thus limiting the accumulation of excess heat. Precontouring is put forward as a scanning strategy to improve the manufacturability of thin sections.

Facies Analysis and Depositional Environment of the Euphrates Formation in Selected Sections, Southwestern Iraq

The current study aims to investigate the microfacies, diagenetic processes, and depositional environments of the twenty-three rock samples from the Lower Miocene Euphrates Formation of two outcrops collected at Bahar-Najaf in Najaf governorate and Al-Shinafiya in Diwaniyah governorate, southern Iraq. Thin sections of these samples were prepared; hence, the nature of microfacies and related depositional environments was established. The formation contains abundant benthonic foraminifers and other fossils like miliolids, echinoderm, coral, gastropods, and pelecypods. Seven distinct microfacies were identified: lime mudstone, miliolid packstone, Pelletal Grainstone, Lime Wackestone, Peloidal Grainstone, Oolitic grainstone Microfacies, and fossiliferous packstone packed with bioclasts. Diagenetic processes evidenced in this study are dissolution, neomorphism, cementation, micritization, and dolomitization. The Euphrates Formation was deposited in a shallow marine setting with fluctuating energy levels, ranging from low-energy lagoons to moderate and high-energy shoals and fore-reef environments. Various microfacies indicate a complex depositional environment on a carbonate platform influenced by biota and sedimentary processes. These observations support the inference that the Euphrates Formation accumulated in shallow open marine, inner barrier, and shoal settings, providing a detailed interpretation of its para-depositional and diagenetic development.

Giant plagioclase in the Steens Basalt, southeast Oregon, USA: Tracking the balance of differentiation processes in continental flood basalt evolution

Giant plagioclase basalts (GPBs), flows that contain abundant and conspicuous plagioclase crystals ≥1 cm in length, exist at many continental flood basalt provinces, and their presence can provide a window into the crustal differentiation processes and storage conditions that produced the magmas prior to eruption. GPB flows occur in the Steens Basalt of southeast Oregon, USA, one of the earliest members of the Columbia River Flood Basalt Group, but their origin in this province has not been studied in detail. We conducted quantitative textural analysis utilizing crystal size distributions (CSDs) at the outcrop and thin section scale, as well as in situ plagioclase trace element and isotopic compositional analysis, in order to elucidate the processes responsible for generating two end-member textural types of GPB found in the Steens Basalt: daisy stone glomerocryst clusters composed of radiating plagioclase laths and single isolated plagioclase. CSD patterns suggest a changing balance of multiple differentiation processes affected the magmas that created each GPB textural type. In the daisy stone plagioclase, crystals rarely exhibit resorption textures, trace element abundances are comparatively low with minimal variability, and Sr isotopes are commonly in equilibrium with groundmass. In the single isolated plagioclase, resorption textures are common, trace element abundances are generally higher and display within crystal variation, and Sr isotopes are typically not in equilibrium with groundmass. These distinctive characteristics require separate mechanisms to generate each end-member GPB texture. Daisy stone GPBs form by growth of large crystals following mafic recharge events that completely resorb entrained cumulate plagioclase. A large amount of plagioclase may then grow with minimal cooling following recharge due to saturation in plagioclase components. In contrast, single isolated GPBs are the result of partial resorption of both entrained and resident plagioclase following less voluminous and less primitive recharge events; regrowth then occurs on the partially resorbed cores when cooling resumes. Both GPB types are formed by thermal cycling, but each texture reflects the differentiation processes that were prevalent during different eruptive stages of the Steens Basalt, as the balance between recharge, crystal fractionation, and assimilation varied in time.

Characteristics and main controlling factors of the Sangonghe tight sandstone reservoirs in the Shengbei Sub-sag of the Turpan-Hami basin, China

The Sangonghe tight sandstone hydrocarbon reservoirs in the Shengbei Sub-sag of the Turpan-Hami Basin are key areas for hydrocarbon exploration. However, previous research has mostly focused on petrological characteristics, physical properties, and pore types. There is a lack of understanding regarding diagenesis, microscopic pore-throat features, and the impact of overpressure on the reservoir. This gap in knowledge poses a disadvantage for future studies and hydrocarbon exploration. This study examines the Sangonghe tight sandstone hydrocarbon reservoirs in the Shengbei Sub-sag of the Turpan-Hami Basin. It combines cast thin sections, physical property tests, high-pressure mercury injection experiments, low-temperature nitrogen adsorption experiments, and well logging overpressure identification to investigate the basic characteristics, diagenesis, and microscopic pore-throat features of these reservoirs. Based on this analysis, the study identifies the main controlling factors of the reservoir properties and presents three key findings. First, the reservoir rocks are primarily composed of lithic sandstone and feldspathic lithic sandstone with porosity ranging from 4% to 10% and permeability ranging between 0.1 and 10 mD, classifying it as a low-porosity, low-permeability tight sandstone reservoir. Second, the reservoir has undergone several diagenetic processes, with significant compaction and common occurrences of cementation and metasomatism. Acidic dissolution is the main dissolution process, but it is relatively weak. The pore-throat system exhibits poor and concentrated sorting, with nanometer-sized pores being predominant. These include 'ink-bottle' pores with narrow necks and wide bodies, and parallel plate-structured fissure pores. Finally, the development of the reservoirs is mainly influenced by sedimentation, dissolution, and overpressure. The parent rock provenance features a high content of stable heavy minerals, larger grain sizes, and thicker sand body layers. These factors enhanced the reservoir's resistance to compaction and promoted the development of primary intergranular pores. Additionally, organic acid fluids caused extensive dissolution of feldspar and lithic fragments, resulting in the formation of numerous secondary dissolution pores. Additionally, under-compaction overpressure helped preserve primary pores, while hydrocarbon generation overpressure extended the hydrocarbon generation period. This extended period provided the driving force for fluid migration, enhanced the transformation of reservoir space, and aided in hydrocarbon accumulation.

INFLUENCE AND PREDICTION OF SINTERED AGGREGATE SIZE DISTRIBUTION ON THE PERFORMANCE OF LIGHTWEIGHT ALKALI-ACTIVATED CONCRETE

The most effective method for mitigating the adverse environmental impacts of traditional concrete involves substituting cement and natural aggregate with waste and byproduct resources. Utilizing sintered lightweight aggregate (fly ash) in geopolymer concrete emerges as an efficient solution for managing and disposing of significant amounts of fly ash. The influence of sintered aggregate size distribution on the performance of alkali-activated concrete, focusing on compressive strength improvement. The study employs sintered fly ash aggregate (SFA) as coarse aggregate, aiming to optimize packing density through proper particle distribution. The highest compressive strength is achieved with a mix featuring 75% 4-8mm and 25% 8-12mm SFA. Regression-based strength models are developed, exhibiting good alignment with conventional concrete models. Thin section techniques reveal enhanced aggregate-matrix interaction due to the porous structure of SFA. The study emphasizes the potential of SFA in geopolymer concrete for sustainable construction. Lightweight geopolymer concrete, owing to its lower density, significantly reduces the overall structural load.

Quantitative characterization of porosity evolution in the Triassic Chang 8 tight sandstone reservoir during hydrocarbon accumulation in the Maling area, Ordos Basin, China

The Maling area in the Ordos Basin is characterized by delta front deposits. The principal oil-bearing layer, Chang 8 Member (the 8th member of the Triassic Yanchang Formation), manifests low-to-ultra-low porosity and permeability due to the interplay of depositional, diagenetic, and burial processes. The reservoir exhibits pronounced heterogeneity and diagenetic alterations, which limit the efficacy of hydrocarbon exploration. Methodologies employed for this research encompass grain size imaging, physical property assays, petrographic thin sections, scanning electron microscopy, high-pressure mercury intrusion, and X-ray diffraction. These techniques facilitated a comprehensive investigation into the diagenetic attributes and compaction mechanisms of the reservoir. A porosity evolution simulation equation tailored to the Chang 8 tight sandstone reservoir was formulated based on diagenetic evolution traits and geological influences. Interdependencies and correlations among various diagenetic processes were analyzed. During diagenesis, compaction, cementation, and replacement led to a reduction in primary porosity, whereas dissolution-induced secondary pores served as high-permeability channels. A quantitative model for porosity evolution was developed alongside qualitative assessments, achieving a deviation of 3.18% when compared to gas porosity measurements. Evaluation of four representative samples revealed that compaction-induced diagenetic alteration predominates, with burial depth, formation age, and cement types playing critical roles in the evolution of reservoir porosity. This differential diagenetic evolution elucidates the underlying causes of variability in reservoir properties and heterogeneity in pore structure, providing valuable insights for future exploration strategies.

Lithological and Structural Mapping of Basement Rock Units in the Ikara North-Central Basement Complex, Nigeria

Study’s Excerpt The lithological and structural composition of the Ikara North-Central Basement Complex in Nigeria is investigated. Detailed field and petrographic analyses were carried out to identify the rock types of the area. The geological map of the area is updated. Full Abstract The lithological and structural architecture to unravel the compositions and tectonic evolution of the Nigerian Basement Complex has not been fully documented by geoscientists. As such, this research aimed to investigate the lithological and structural characteristics of the Ikara North-Central Basement Complex. Detailed petrographic and field analysis reveals the prevalence of gneiss and granite lithologies characterized by significant structural features such as fractures, folds, and foliations. These findings offer new understandings of the tectonic evolution of the region. The study area mapped is the Ikara Sheet 103 Federal Survey Topographic Sheet to a scale of 1:100,000. The major lithological units underlying the study area are gneisses and granites. However, these major rock types have varieties, namely migmatitic gneiss, granite gneiss, migmatitic (augen) gneiss, porphyroblastic gneiss, medium to coarse-grained granites, and porphyritic granites. These rock types were intruded by quartzites, pegmatitic dykes, quartz, and aplite veins. Rock samples were observed and studied based on their mineralogical composition, color, and texture. The results of the petrographic analysis showed that the rocks are mainly composed of quartz, plagioclase feldspars, alkali feldspars, mica (biotite and muscovite), hornblende, garnet, and accessory minerals like zircon and magnetite. Geological structures were also observed during the field study, namely fractures (joints and fractures), foliations, lineations, veins, dykes, and various landforms. Photomicrographs from thin section analyses revealed micro-structures like myrmekites, micro-folds, and micro-fractures were also observed. Mineralization such as amethyst and cassiterite were hosted by quartz veins and pegmatites in porphyritic granites, respectively. A litho-structural map was produced, exhibiting the lithological units and regional structures cross-cutting the area of research.

Insights into the wetting mechanisms in low-permeability sandstone reservoirs and its evolution processes: The shahejie formation in the Dongying Depression, Bohai Bay basin

During the accumulation and development processes of low-permeability oil, wettability plays a crucial role in the percolation capacity of fluids. In particular, the evolution of low-permeability sandstones involves complex changes in formation fluid properties and mineral types, leading to a deficient understanding of wetting mechanisms in low-permeability sandstones. This gap significantly impedes research on the accumulation mechanisms of low-permeability oil. Focusing on the low-permeability sandstones in Shahejie formation of Dongying Depression, Bohai Bay Basin, tests like Casting thin section, scanning electron microscope, contact angle measurement, molecular dynamics simulation and the Amott method based on nuclear magnetic resonance technology were performed to comprehensively investigate sandstone wettability and its evolutionary process. The results indicate that the adsorption capacity of hydroxyl groups or monovalent cations (K+and Na+) for water molecules causes residual intergranular pores, feldspar dissolution pores, quartz dissolution pores, clay mineral intercrystalline pores and micro-cracks to exhibit hydrophilicity; while the adsorption capacity of divalent cations (Ca2+and Mg2+) for oil molecules causes the surface of dissolution pores of carbonate minerals to exhibit neutrality or lipophilicity. Additionally, changes in formation conditions (temperature, pressure, ion types, and ion concentration) significantly control the wettability of pore surfaces, further determining the overall wettability of low-permeability sandstone reservoirs. Throughout the evolutionary process of low-permeability sandstones, the Amott-Harvey index of low permeability sandstone is greater than zero, and the wettability exhibited hydrophilic or neutral. From A-stage eodiagenesis to B-stage mesodiagenesis, the Amott-Harvey index is 0.82, 0.12, 0.37, 0.03, and 0.49, and the wettability of reservoirs transitions through phases of strong hydrophilicity, weak hydrophilicity, hydrophilicity, neutrality, and hydrophilicity, respectively. Finally, an evolutionary model of the wettability of low-permeability sandstone reservoirs was established, which is of great significance for predicting the quality of low-permeability sandstone reservoirs.

A refined TTC assay precisely detects cardiac injury and cellular viability in the infarcted mouse heart

Histological analysis with 2,3,5-triphenyltetrazolium chloride (TTC) staining is the most frequently used tool to detect myocardial ischemia/reperfusion injury. However, its practicality is often challenged by poor image quality in gross histology, leading to an equivocal infarct-boundary delineation and potentially compromised measurement accuracy. Here, we introduce several crucial refinements in staining protocol and sample processing, which enable TTC images to be analyzed with light microscopy. The refined protocol involves a two-step TTC staining process (perfusion and immersion) and subsequent Zamboni fixation to differentiate myocardial viability and necrosis, and use of Coomassie brilliant blue to label area-at-risk. After the duo-staining steps were completed, the heart sample was embedded and sliced transversally by a cryostat into a series of thin sections (50 µm) for microscopic analysis. The refined TTC (redTTC) assay yielded remarkably high-quality images with striking color intensity and sharply defined boundaries, permitting unambiguous and reliable delineation of the infarct and area-at-risk. In the same animals, the redTTC assay showed good agreement with the in-vivo gold standard measurements (LGE and MEMRI). Meanwhile, redTTC imaging allows tracking of viable cardiomyocytes at cellular resolution, and with this enhanced capability, we convincingly demonstrated the pro-survival action of stem cells based-therapy. Therefore, the redTTC assay represents a significant technical advance that permits precise detection of the true extent of cardiac injury and cardiomyocyte viability. This approach is cost-effective and may be adapted for use in diverse applications, making it highly appealing to many laboratories performing ischemia/reperfusion injury experiments.

Mineralogical Characteristics and Their Usability as Gemstones of Jaspers in Altered Metavolcanics Belonging to the Topçam Formation, Tokat, Turkiye

The jaspers located in the Topçam Mountain, Tepeyurt region (Tokat, Turkey), were formed at green-colored metavolcanic levels within Permian–Triassic schists. The sizes of the jaspers generally vary from a few cm to 1.5 m, and their colors vary from grayish–reddish–brownish/blackish tones depending on the increase in the amount of Fe and Mn elements they contain. According to thin section and X-ray Diffraction (XRD) analysis examinations, it was determined that the composition of jaspers includes quartz, hematite, calcite, and pyrite. As stated by the Wavelength Dispersive X-ray Fluorescence (WDXRF) analysis results, it was observed that the amount of SiO2 in jaspers was 82.5%, and the amount of Fe2O3 was 15.5%. According to Inductively Coupled Plasma-Mass Spectrometry (ICP-MS) analysis results, Fe and Mn impurities, which are thought to cause the color of jaspers, as well as the amount of Fe (6975.5 ppm–46,893.9 ppm–96,431.1 ppm) and the amount of Mn (935.9–3219.7–6040.4 ppm), caused a darkening in color tones (grayish–reddish–brownish/blackish). Cabochon cutting applications were made of jaspers taken from the study area. As a result of these applications, it has been determined that jaspers can be used as gemstones due to their properties, such as their workability, polish retention, and color and light reflection.

Multiscale Characterization of Fractures and Analysis of Key Controlling Factors for Fracture Development in Tight Sandstone Reservoirs of the Yanchang Formation, SW Ordos Basin, China

Tight sandstone reservoirs, despite their low porosity and permeability, present considerable exploration potential as unconventional hydrocarbon resources. Natural fractures play a crucial role in hydrocarbon migration, accumulation, and present engineering challenges such as late-stage reformation in these reservoirs. This study examines fractures in the seventh member of the Triassic Yanchang Formation’s tight sandstone within the Ordos Basin using a range of methods, including field outcrops, core samples, imaging and conventional logging, thin sections, and scanning electron microscopy. The study clarifies the characteristics of fracture development and evaluates the relationship between dynamic and static rock mechanics parameters, including the calculation of the brittleness index. Primary factors influencing fracture development were quantitatively assessed through a combination of outcrop, core, and mechanical test data. Findings reveal that high-angle structural fractures are predominant, with some bedding and diagenetic fractures also present. Acoustic, spontaneous potential, and caliper logging, in conjunction with imaging data, enabled the development of a comprehensive probabilistic index for fracture identification, which produced favorable results. The analysis identifies four key factors influencing fracture development: stratum thickness, brittleness index, lithology, and rock mechanical stratigraphy. Among these factors, stratum thickness is negatively correlated with fracture development. Conversely, the brittleness index positively correlates with fracture development and significantly influences fracture length, aperture, and linear density. Fractures are most prevalent in siltstone and fine sandstone, with minimal development in mudstone. Different rock mechanics layer types also impact fracture development. These insights into fracture characteristics and controlling factors are anticipated to enhance exploration efforts and contribute to the study of similar unconventional reservoirs.

Advanced three-dimensional X-ray imaging unravels structural development of the human thymus compartments

BackgroundThe thymus, responsible for T cell-mediated adaptive immune system, has a structural and functional complexity that is not yet fully understood. Until now, thymic anatomy has been studied using histological thin sections or confocal microscopy 3D reconstruction, necessarily for limited volumes.MethodsWe used Phase Contrast X-Ray Computed Tomography to address the lack of whole-organ volumetric information on the microarchitecture of its structural components. We scanned 15 human thymi (9 foetal and 6 postnatal) with synchrotron radiation, and repeated scans using a conventional laboratory x-ray system. We used histology, immunofluorescence and flow cytometry to validate the x-ray findings.ResultsApplication to human thymi at pre- and post-natal stages allowed reliable tracking and quantification of the evolution of parameters such as size and distribution of Hassall’s Bodies and medulla-to-cortex ratio, whose changes reflect adaptation of thymic activity. We show that Hassall’s bodies can occupy 25% of the medulla volume, indicating they should be considered a third thymic compartment with possible implications on their role. Moreover, we demonstrate compatible results can be obtained with standard laboratory-based x-ray equipment, making this research tool accessible to a wider community.ConclusionsOur study allows overcoming the resolution and/or volumetric limitations of existing approaches for the study of thymic disfunction in congenital and acquired disorders affecting the adaptive immune system.

Bone microstructure analyses in ontogenetic series of Mesosaurus tenuidens from the early Permian of Brazil.

Osteohistological evidence is widely used to infer paleobiological traits of fossil vertebrates, such as ontogeny and growth rates. Mesosaurs, an enigmatic group of aquatic reptiles from the early Permian, are the most well-known Paleozoic amniotes from Africa and South America. Their fossils are abundant in South America, ranging from the central-west region of Brazil to the southernmost areas, as well as parts of Paraguay and Uruguay. In this contribution, we examined the bone microstructure of Mesosaurus tenuidens by analyzing thin sections of axial and appendicular elements of several specimens collected from various Brazilian sites. The microstructure of the bones showed minimal histological variability among elements, predominantly composed of parallel-fibered tissues, indicating slow growth rhythm, along with increased bone density attributed to pachyosteosclerosis. The cortical area consists of poorly vascularized parallel-fibered bone tissue, which was interrupted by multiple cyclical growth marks, some of them being supernumerary, suggesting a strong influence of seasonality. Moreover, the organization of growth marks suggests distinct life history trajectories among individuals collected from different outcrops, reflecting environmental heterogeneity throughout the basin. Internally, the endosteal domain exhibits greater vascularization compared to the cortices and frequently contained calcified cartilage. In the ontogenetic series, there was a progressive filling of the medullary region from small to large individuals. The presence of the External Fundamental System (a proxy indicating somatic maturity) was observed in femora and ribs, suggesting that determinate growth was already occurring in Permian mesosaurs and may not be an exclusive specialization of crown amniotes.