Thickness Of Tunica Muscularis Research Articles

Dandelion Root Extract in Trout Feed and its Effects on the Physiological Performance of Oncorhynchus mykiss and Resistance to Lactococcus garvieae Infection

Abstract In this study, we investigated the dietary effects of dandelion root extract (DRE) on growth performance, body composition, serum biochemical parameters, intestinal and liver histology, and fish resistance against Lactococcus garvieae infection in the rainbow trout. In total, 540 rainbow trout fry (22.05±1.740 g and 13.03±0.470 cm) were used. Six experimental groups (control, 2.5 mL/kg (DRE-1), 5 mL/kg (DRE-2), 10 mL/kg (DRE-3), 20 mL/kg (DRE-4), and 40 mL/kg (DRE-5) dandelion root extract) with three replicates were formed. In each tank, 30 fish were placed and fed for 90 days, and the volume of water was adjusted to 80 L with a flow-through system. The results showed that the final fish weight, weight gain, specific growth rate, protein efficiency ratio, and protein deposition rate were significantly higher in the DRE-4 group, while the feed conversion ratio and fat deposition ratio were significantly lower compared to that in the control group (P<0.05). The viscerosomatic index and the hepatosomatic index decreased in the experimental groups depending on the concentration of the extract used, while the condition factor was higher than that in the control (P<0.05). The DRE significantly decreased glucose, cholesterol, triglyceride, low-density lipoprotein, alanine aminotransferase, and aspartate aminotransferase levels in blood serum in the fish from the experimental groups, but it significantly increased high-density lipoprotein, alkaline phosphatase, and total protein levels (P<0.05). Similarly, the DRE positively affected intestinal histology by increasing the stratum compactum, submucosa, lamina propria, villi length, villi width, absorption area, tunica muscularis thickness, mucosal folds, and goblet cell count. In the liver, depending on the high-fat diet (crude lipid = 20%), vacuolization areas, hepatocellular degeneration areas, apoptotic hepatocyte nucleus, and necrotic areas recorded in the control group decreased or even disappeared completely in the extract groups. The results of the challenge test showed that adding 2.5 mL/kg of the DRE to the feed positively affected the disease resistance against Lactococcus garvieae infection. The results of the quadratic polynomial regression showed that an average level of 23.91±1.048 mL/kg of DRE would be suitable for optimum fish growth.

Anatomical, histological, and histochemical alterations in portio vaginalis uteri with an evaluation of the vaginal artery vascularity during the luteal and early pregnant stages in domestic buffaloes (Bubalus bubalis)

The portio-vaginalis uteri (PVU) and its mucus secretion have shown an essential role in conception. A significant endeavour to improve buffaloes' reproductive efficiency is the investigation of their basic reproductive pattern, which provides a reference for applications in breeding and pregnancy. The present study aimed to evaluate the anatomical and histological alterations in PVU regarding to the vaginal artery (VA) hemodynamic at luteal and early pregnant stages in buffalos. Egyptian live buffaloes (n = 16) and fresh genitals (n = 25) of mature buffalo were used. Different luteal and early pregnant stages were macroscopically identified with the shape and mucosal colouration with discharges of the PVU. Histological examination showed a significant difference in area % of alcian blue and periodic acid Schiff positive granules which considered an indication for presence of acidic and neutral mucins respectively in the epithelial cells of PVU mucosa which increased in pregnant stage than in other luteal stages. VA assessment demonstrated an increase in luminal diameter and thickness of tunica muscularis in pregnant stage than other stages (P < 0.05). Middle uterine (MUA) and VA arteries peak velocity point (PSV mm/sec) were elevated (P < 0.05) in pregnant stage, with a marked reduction in both resistance and pulsatility indices (RI and PI), and ratio of systolic /diastolic (S/D). Positive correlation was detected between VA. PSV and, MUA. PSV (r = 0.87), but a negative relation was detected with VA. S/D (r = -0.77), VA.PI (r = -0.89), VA. RI (r = -0.97), MUA. S/D (r = -0.94), MUA. PI (r = -0.85), and MUA. RI (r = -0.88). Doppler indices were negatively corrected with the VA. PSV (r = -0.68). It was concluded that there was a significant alterations in histological features of the cervical PVU at different physiological stages (luteal and early pregnant) in buffalos in relation to the MUA and VA hemodynamic pattern and that hypotheses can be established regarding the female cyclicity that affected by both arteries hemodynamics change.

Effects of In-Ovo Glucose and Glutamine Treatment on Hatching Efficiency, Intestinal Histomorphology and Gene Expression of Digestive Enzymes in Broiler Chicks

The purpose of the present study was to identify the effects of in-ovo injection of glucose and glutamine on hatching efficiency, hatching weight, liver and residual yolk weights, intestinal histomorphology and gene expressions of digestive enzymes. On the 17th day of incubation, NaCl (0.9 %), glutamine (0.5 ml 10%), glucose (0.5 ml 0.25 g/ml), and glutamine+glucose (0.25 ml 10%+0.25 ml 0.25 g/ml) were injected into the amniotic sac. In-ovo injection of glucose, glutamine and glucose+glutamine significantly decreased hatching efficiency in glucose and glutamin+glucose groups (p&lt;0.001). This treatment did not affect hatching weight, liver and residual yolk weight in any of the groups. When the intestinal histomorphology was evaluated, in-ovo injection was found to increase the villi height/crypt depth ratio, villus width and the number of goblet cells in the jejunum; whereas villi height, crypt depth, and tunica muscularis thickness were not significantly affected by the treatments. Moreover, the ileum histomorphology in general appear not to be affected by any of the treatments as well. In-ovo glutamine injection significantly increased Suc-Iso and mTOR gene expressions (p≤0.05) compared to the control group, whereas SGLT1 gene expression was statistically indifferent. At the end of the study, while in-ovo glucose and glutamine injection had an overall negative effect on the hatching efficiency in broiler chicks.

Histological study of oviduct in adult Iraqi Black Goat

The present study was aimed at the light microscopic examination of the oviduct and show epithelium structure of the iraqi goat oviduct, determination of the histochemical structure of the mucus secreted by epithelial cells, and muscular layers of The oviducts of Twelve healthy adult goat constituted the material of the study. using routine histological techniques by using Hematoxylin and eosin , masson trichrom for Histochemically, it was observed by using Pas Alcian Histological observations revealed that the oviduct consists of 4 layers of mucosa, submucosa, tunica muscularis and serosa. The primary and secondary folds increase both in number and in height in infundibulum and ampulla while less in number in isthmus. Epithelium of folds was composed of simple columnar, although seems pseudostratified in some areas, and contains ciliated and secretory cells. Histomorphometric examinations of three regions demonstrated that the mean height of primary folds increase and the mean thickness of tunica muscularis in isthmus The mean thickness of mucosa-submucosa in ampulla More visibility of the ciliated cells and mucosal folds in infundibulum and ampulla the thick tunica muscularis in isthmus transports sperm cells up; and both require promoting secretory products were increased in the tip of the mucosal fold than in the base. Conclusion By using light microscopic examinations lined by pseudo-stratified columnar epithelium. Mucosal folds were maximum in number in the tubual part of infundibulum, ampulla and least in isthmus in all stages of the animal. The propria–submucosa was comprised of loose connective tissue with blood vessels, abundant amount of collagen fibres and few reticular fibers histochemical staining demonstrated by means of the PAS reaction, and it was observed that secretion increased progressively towards the isthmus.

Milk Formula Enriched with Sodium Butyrate Influences Small Intestine Contractility in Neonatal Pigs.

Butyrate, a by-product of gut bacteria fermentation as well as the digestion of fat in mother’s milk, exerts a wide spectrum of beneficial effects in the gastrointestinal tissues. The present study aimed to determine the effects of sodium butyrate on small intestine contractility in neonatal piglets. Piglets were fed milk formula alone (group C) or milk formula supplemented with sodium butyrate (group B). After a 7-day treatment period, isometric recordings of whole-thickness segments of the duodenum and middle jejunum were obtained by electric field stimulation under the influence of increasing doses of Ach (acetylocholine) in the presence of TTX (tetrodotoxin) and atropine. Moreover, structural properties of the intestinal wall were assessed, together with the expression of cholinergic and muscarinic receptors (M1 and M2). In both intestinal segments (duodenum and middle jejunum), EFS (electric field stimulation) impulses resulted in increased contractility and amplitude of contractions in group B compared to group C. Additionally, exposure to dietary butyrate led to a significant increase in tunica muscularis thickness in the duodenum, while mitotic and apoptotic indices were increased in the middle jejunum. The expression of M1 and M2 receptors in the middle jejunum was significantly higher after butyrate treatment. The results indicate increased cholinergic signaling and small intestinal growth and renewal in response to feeding with milk formula enriched with sodium butyrate in neonatal piglets.

The histological and histochemical features of the esophagus in local breed dogs (Canis familiaris)

A total of eight samples of the esophagus of local breed dogs are used to investigate the histochemical features of the esophagus. The specimens are processed according to paraffin embedding technique protocols and the tissue sections are stained with hematoxylin and eosin, and masons trichrome combined with Alcian blue (pH2.5)-PAS. The esophagus folds the mucosa which is covered by keratinized to non-keratinized stratified squamous epithelium which contains numerous excretory common ducts of esophageal glands. The epithelial thickness of cervical, thoracic and abdominal parts of the esophagus are 221.95±3.41µm, 212.46±5.38 µm, and 173.15±4.09 µm respectively. The lamina propria of the cervical part of the esophagus lacks the muscularis mucosa, while the muscular mucosa in the thoracic part of the smooth muscles appears as scattered interrupted bundles. The esophageal glands are a type of compound tubular mucoserous constructed of the predominated mucous alveoli and little serous acini. When combined with Alcian blue (pH2.5)-PAS stain, the esophageal glands show strong acidic mucopolysaccharides within the mucous alveoli and light blue color within serous acini that denote weak acidic zymogen granules. Tunica muscularis is striated muscle fibers in cervical and thoracic parts and smooth in the abdominal part of the esophagus. The measured thickness of tunica muscularis of cervical, thoracic and abdominal parts are 568.76±6.90 µm, 703.29±7.54µm, and 338.98±7.26 µm respectively.

Effects of oral glutamine supplementation on jejunal morphology, development, and amino acid profiles in male low birth weight suckling piglets.

BackgroundIt has been shown that small intestine development in low birth weight (LBW) piglets is impaired. Glutamine (Gln) has been reported to improve piglet health and intestinal function in weaned piglets, but data is scarce in suckling piglets. This study was conducted to investigate the effects of oral Gln supplementation compared to Alanine (Ala) on jejunal development and function in 5 and 12 d old male LBW and normal birth weight (NBW) suckling piglets.ResultsGln had no effect on the jejunal morphology, development, tissue and digesta amino acid profiles and mRNA abundance of genes involved in amino acid transport, metabolism, glutathione synthesis in LBW piglets when compared to Ala supplementation and birth weight controls at 5 and 12 d. Only the concentration of Gln in jejunal tissue was higher in NBW piglets supplemented with Gln compared to Ala at 5 d (P < 0.05). A comparison of the birth weight groups showed no differences between LBW and NBW piglets at 5 and 12 d in any parameter. Jejunal crypt depth, villus height / width, tunica muscularis thickness, number of goblet and IgA positive cells, the ratio of jejunal RNA to DNA and the concentration of DNA, protein and RNA changed (P < 0.05) from 5 compared to 12 d. The concentrations of several free, and protein bound amino acids as well as amino metabolites differed between age groups in jejunal tissue but the digesta concentrations were affected to a lesser extent.ConclusionsOral Gln supplementation to suckling male piglets over the first 12 d of life was not associated with changes in jejunal parameters measured in this study. The absence of effects may indicate that Gln is absorbed as well as metabolized in the upper intestinal tract and thus could benefit intestinal development at a more proximal location.

The effect of herbal extract mixture (Digestarom) supplementation to diets on duodenum and ileum histology in broiler chickens

Phytogenic feed additives are used as antibacterial, anti-inflammatory and performance enhancer in the nutrition of broiler chicks. In the present study, it was aimed to investigate the effects of the usage of phytogenic feed additives which are used as an alternative to antibiotics in broilers at different levels in the rations, on the morphology of the duodenum and ileum. For this purpose, 48 male ROSS-308 one-day-old broiler chicks were used in the study. Chicks with a body weight of 43±3 g were randomly divided into 24 replicate sets from three experimental groups, each of which consisted of 8 replicates (2 chicks per house). The other two experimental groups were fed with a basal diet supplemented with 100 mg/kg (DIG100) and 150 mg/kg (DIG150) phytogenic product Digestarom®. In conclusion, it was determined that herbal extract mixture supplementation to broiler chick rations generally increased villus height and villus width, and decreased tunica muscularis thickness. Hence, herbal extract mixture might lead to better digestion and absorption by increasing the intestinal surface area, digestion and absorption levels, also increase body weight gain in broiler chicks by reducing the microbial load.

The Effect of a Diet Containing Extruded Faba Bean Seeds on Growth Performance and Selected Microbial Activity Indices in the Large Intestine of Piglets.

Simple SummaryGrain legumes are cultivated for food and feed purposes in all regions of the world. Legumes are the main source of protein for a large part of the world population where animal protein is hardly available. The potential of grain legumes is increasing primarily due to the genetic improvement of their agricultural and nutritional characteristics and expansion of organic farming. They are also fed to animals as a component of concentrates and on farms producing “organic” food. Therefore, studies on the composition, nutritional value and factors affecting quality of legume protein contribute to a more efficient utilization of seeds as feed and food ingredients. Faba bean is rich in both starch and protein and is an important alternative protein source in animal nutrition; however, its potential is not yet fully exploited, particularly in pig diets. The aim of the study was to evaluate the effect of diets containing various levels of extruded faba bean seeds on growth performance and selected microbial activity indices in the large intestine of pigs. Treatments with faba bean seeds did not negatively affect growth performance (except for the highest level of faba bean) and microbial activity in the large intestine, and can be applied in piglet diets.The study investigated the effect of replacing soybean meal with extruded faba bean seeds on piglet growth performance and selected microbial activity indices in the large intestine. In total, 24, 35-day-old, healthy, castrated piglets of similar body weight were divided into four groups with six replicates. Animals in the control group (C) were fed with soybean meal without extruded faba bean seeds. In other experimental groups, pigs were fed diets with the addition of 20 (FB20), 25 (FB25) or 30% (FB30) extruded faba bean seeds instead of soybean meal and wheat starch. Growth performance, histology of the large intestine, short-chain fatty acids (SCFA) and ammonia concentration, as well as the activity of bacterial enzymes in digesta samples, were analyzed. The intake of the FB25 diet resulted in an increased feed:gain ratio in comparison to the FB30 group. Feeding the FB30 diet increased tunica muscularis thickness in the caecum as compared to other groups. Moreover, dietary inclusion of extruded faba bean seeds had no effect on SCFA and ammonia concentration. In addition, feeding diets with a different level of extruded faba bean seeds did not affect the activity of bacterial enzymes in the colon.

Effects of diets with different solid-to-liquid feed ratios with the same dry matter intake on the growth performance and gastrointestinal development of male Holstein calves

This study investigated the effects of diets with different solid-to-liquid feed ratios with the same dry matter intake on growth performance, blood metabolites, rumen development, and gastrointestinal development in male Holstein calves. Calves (n = 36; average 28 ± 2 days of age; 46.4 ± 0.78 kg body weight) were randomly assigned to three groups: the control group (n = 12; solid to liquid feed ratio = 1:2 at 28 days of age (d 28), then, the solid feed supply was increased by 200 g per week until the ratio was changed to about 1:1 at d 56), high liquid (HL) group (n = 12; solid to liquid feed ratio = 1:2 at d 28, then, the solid feed supply was increased by 100 g per week until the ratio was still about 1:2 at d 56), and high solid (HS) group (n = 12; solid to liquid feed ratio = 1:2 at d 28, then, the solid feed supply was increased by 400 g per week until the ratio was changed to about 1:0 at d 56). The calves were reared with three different feed types while maintaining the same dry matter feed supply and dry matter intake among the three groups before weaning. The calves from both the control and HL groups were weaned at 56 days of age with approximately 1 kg and 0.5 kg of daily solid feed intake, respectively. The calves from the HS group were weaned at 42 days of age with approximately 1 kg of daily solid feed intake. Calves were weighed at 2-week intervals, and both blood and rumen fluid samples were also collected at 2-week intervals. Calves were slaughtered, and various organs were collected for analysis. Compared with the control and HL diets, the HS diet increased the daily solid feed intake. The average daily gain did not differ among the three groups until 56 days of age, but the HS diet increased the average daily gain compared to the HL diet from 56 to 84 days of age. Compared with the HL diet, the HS diet increased the ruminal molar proportion of acetate and butyrate and the total volatile fatty acid concentration at 84 days of age. Compared with the HL diet, the HS diet improved blood IgG and insulin-like growth factor 1 concentrations, and increased the weights of the rumen and whole stomach and increased the papilla length and tunica muscularis thickness of the rumen, especially the tunica muscularis thickness of the duodenum. In conclusion, feeding an HS diet along with early weaning at 42 days of age increased solid feed intake and resulted in improved growth performance, molar proportion of butyrate in the rumen, blood immune index levels and gastrointestinal development of post-weaned Holstein male calves.

Histomorphological and Histochemical Investigation of The Vagina of Adult Guinea Pigs (Cavia porcellus)

This study was carried out to identify the morphological, histological and histochemical features of the vagina of the adult guinea pig (Cavia porcellus). To perform such project, 14 adult guinea pigs at their diestrous period were bought from the local breeders directly. Animals were euthanized, dissected and subsequently specimens from the cranial, middle and caudal portions of the vagina were collected and fixed. Fixation was implemented by using 10% neutral buffered formalin and Bouin’s solution. Post routine processing such as dehydration, clearing, embedding and blocks preparation, tissue sections of 6 μm were prepared and stained by using hematoxylin-eosin, Masson’s Trichrome, Alcian blue and Periodic acid shiff stains. Gross findings revealed that the vagina in the adult guinea pigs was characteristically very long tube-like structure with wide diameter. The vagina entirely was running ventral to the colon and then under the rectum in the pelvic cavity. It was terminated caudally by the vaginal orifice independent to the urethral orifice of the urinary system. Microscopic findings revealed mucous columnar lining epithelium which was folded at the vaginal fornix decreased caudally toward the external vaginal orifice where the epithelium changed into stratified squamous epithelium not keratinized. Thin dense lamina propria was continuous with loose connective tissue of the submucosa. Thickness of tunica muscularis was decreased which was surrounded with thick adventitia. Histochemically, the non-ciliated mucous columnar cells present in the lining epithelium of the cranial and middle regions of the vagina were positively stained with AB (pH 2.5) and PAS stains. The reaction with AB (pH 2.5) was more intense compared to the staining with PAS so that it indicated that mucin was more acidic than neutral in nature. Characteristic conclusions include that the vagina opening was U-shaped not circular and closed by transparent closure during the diestrous period. Histologically, the vagina also lined with mucoid lining and only the orifice and adjacent area lined with stratified squamous epithelium. Moreover, current study recorded differences in both macroscopic and microscopic aspects of the vagina in the guinea pigs compared to other animal considered laboratory species.

Effect of Different Dietary Inclusion Levels of Sunflower Meal and Multi-Enzyme Supplementation on Performance, Meat Yield, Ileum Histomorphology, and Pancreatic Enzyme Activities in Growing Quails.

Simple SummaryIn the poultry industry, where the cost of feed constitutes most of the expenses, sudden changes in feed prices make it a challenge for nutritionists to maintain the yield and health of animals while managing the diet costs. It is the first solution that comes to mind to use inexpensive raw materials while preparing diets in practical conditions. In addition, feeds used in diets should be digestible by poultry. In the poultry digestive system, feeds such as sunflower meals are difficult to digest due to containing high cellulose because there is no cellulase secretion in the digestive system. The use of multi-enzyme which enhanced the digestibility of nutrients and decreased antinutritional compounds such as cellulose and nonstarch polysaccharides in diets with sunflower meal can positively affect performance and the profitability of the growers.This study aimed to investigate the effect of dietary sunflower meal (SFM) and multi-enzyme levels on performance, carcass traits, intestinal histomorphology and pancreatic enzyme production in quails. Three hundred and twenty, 1-day-old quail chicks were divided into 8 groups with 4 replicates consisting of 10 birds each in the group. The experiment was randomized design consisting of a 4 × 2 factorial arrangement, with four levels of SFM (0%, 10%, 15%, or 20%) and two levels of multi-enzyme (0.0 or 1.0 g/kg) inclusion in the diet. The body weight, body weight gain, and feed conversion ratio were negatively influenced by the 15% and 20% SFM (p < 0.01) but were not affected by the 10% SFM for 6 week age. The relative gizzard (p < 0.05) weights significantly increased with 20% SFM, but the relative breast weight decreased (p < 0.01). The relative liver weight increased by the addition of enzymes in the diet (p < 0.05). The villus width (p < 0.01) and villus surface area (p < 0.05) of ileum increased linearly with SFM, whereas the villus height (p < 0.01), villus height: crypt depth (p < 0.01) and tunica muscularis thickness (p < 0.01) decreased linearly with SFM. Consequently, it is possible to say that the birds with the least absorptive same weight are the most efficient. The addition of multi-enzyme increased villus height and crypt depth but decreased tunica muscularis thickness of ileum (p < 0.01). Chymotrypsin activity in the pancreas decreased linearly with SFM (p < 0.01). Amylase activity in the pancreas decreased significantly with the addition of the multi-enzyme (p < 0.05). As a result of the study, SFM can be used at a 10% level in growing quail diets with beneficial effects on the absorption surface area. The effects of enzyme supplementation on parameters measured were less pronounced than the SFM inclusion level that higher villus height and lower tunica muscularis thickness were determined in multi-enzyme-fed birds compared to those untreated counterparts.

Kefir ameliorates hypertension via gut–brain mechanisms in spontaneously hypertensive rats

Hypertension is associated with gut dysbiosis and dysregulation of the gut–brain axis. Previous work has shown that probiotic treatments exert beneficial cardiovascular effects in humans and animal models of hypertension. Coupled with the evidence of elevated sympathetic outflow and chronic inflammation in hypertension, we hypothesized that both peripherally and centrally mediated mechanisms underlie the antihypertensive effects of kefir, a probiotic obtained from the fermentation of milk by kefir grains. Eight-week-old spontaneously hypertensive rats (SHRs) were treated by oral gavage with either vehicle or kefir (0.3 ml/100 g/day; 9 weeks; SHR-Kefir), and age-matched with vehicle-treated Wistar Kyoto rats (WKY). Long-term kefir treatment attenuated mean arterial pressure elevations in SHR-Kefir relative to vehicle-treated SHRs. Peripherally, SHRs exhibited differences in the wall of the jejunum (fewer Paneth cells per crypt of Lieberkϋhn and increased tunica muscularis thickness) and higher serum lipopolysaccharide levels compared to WKY, alterations which were reversed in SHR-Kefir. Centrally, kefir treatment reduced IL-6 and TNF-α protein densities, and abolished the microglial activation observed in the hypothalamic paraventricular nucleus and rostral ventrolateral medulla of SHRs. Taken together, our findings indicate that the antihypertensive effects of long-term kefir treatment occur, at least in part, through improved structural and functional integrity of the intestinal wall and protection against neuroinflammation within cardioregulatory nuclei.

Effects of subchronic methionine stimulation on oxidative status and morphological changes in the rat ileum.

This study was conducted to explore the effects of sulfur containing amino acids on redox status and morphological parameters in the rat ileum tissue. Male Wistar albino rats were randomly divided into the following groups: Group K (saline (1 ml/day, i.p.)), Group M (methionine (0.8 mmol/kg/day, i.p.)), Group C (methionine (0.8 mmol/kg/day) + L-cysteine (7 mg/kg/day), i.p.) and Group N (methionine (0.8 mmol/kg/day) + N-acetyl-L-cysteine (50 mg/kg/day), i.p.). Activities of antioxidant enzymes in the ileum were analyzed to profile oxidative status. Morphometric analysis included measurement of villus height (μm), tunica mucosa thickness (μm), tunica muscularis thickness (μm), the total thickness of the ileal wall (μm) and the number of cells in the lamina propria (per 0.1 mm2 of tissue). Results showed that methionine treatment reduced the activity of antioxidant enzymes (SOD, GPx, CAT) and the GSH content compared to the control group (p > 0.05). The application of methionine reduced the following parameters statistically significant compared to the control group: length of the ileal villi (p < 0.01), tunica mucosa thickness (p < 0.01), and ileal wall thickness (p < 0.01). We concluded that methionine induced the changes in the gut redox status, which implied oxidative stress occurrence. L-cysteine and N-acetyl-L-cysteine both exhibited antioxidant properties.

Effects of sugar beet pulp and roasted canola seed, on performance, rumen and small intestine digesta volatile fatty acid concentrations, and small intestine morphology of Arabian lambs

The effects of partial replacement of barley (B) with sugar beet pulp (SBP) with and without roasted canola seed (RCS) on performance, rumen fluids and small intestine digesta volatile fatty acids (VFA) concentrations, digesta viscosity, and small intestine morphology were investigated. Twenty-four Arabian male lambs (118 ± 10 d, 23.7 ± 2.5 kg BW) were fed with diets containing 1) 68% barley (B-RCS); 2) 68% B plus 7% RCS (B + RCS); 3) 36% SBP (SBP-RCS); 4) 36% SBP plus 7% RCS (SBP + RCS). Feeding (fattening) was undertaken for 99 days including 14 day adaptation period. Rumen fluid was collected on d 27, 54, and 81 of fattening period. At slaughter, small intestine tissue samples and digesta were collected. Results showed that lamb performance was not affected (P > 0.05) by carbohydrate source, however it was improved by inclusion of RCS. Lambs fed with SBP had higher (P < 0.05) total VFA and molar proportions of acetate, isobutyrate, and acetate to propionate ratio compared to those lambs fed without SBP diet. Inclusion of RCS increased (P < 0.05) propionate and decreased acetate and isobutyrate in the rumen fluid but there was no interaction of SBP × RCS treatments observed for any of the time points measured. In duodenum and jejunum digesta, lambs fed SBP had higher (P < 0.05) acetate, total VFA molar concentrations, and acetate to propionate ratio than lambs fed without SBP diet. Inclusion of RCS increased (P < 0.05) acetate, total VFA concentration in duodenum and jejunum. Both pH and viscosity of the small intestine digesta in duodenum and jejunum were higher (P < 0.05) in lambs fed SBP than those fed without SBP diet. Partial replacement of barley with SBP increased crypt depth throughout small intestine and also increased thickness of tunica muscularis and villus height in jejunum and ilium. Feeding lambs with diets containing SBP + RCS increased (P < 0.05) number of Brunner's glands, number of villi, and epithelium thickness. In conclusion, partial replacement of barley by SBP along with RCS altered molar proportion of VFA in the rumen, intestine and improved small intestinal morphology of fattening Arabian lambs.

Histomorphometrical changes of oviduct during the long-term exposure of breeder hens to extra thyroxine.

In this research a 100 day long treatment period was considered to unmask the probable adverse effects of long-term induced hyperthyroidism on histomorphometrical attributes of the oviduct in broiler breeder hens. A total of seventy 47-week-old Cobb 500 breeder hens were randomly allotted to two treatment groups (5 replicates of 7 hens each). Thyroxine (T4) was orally administered to the hyperthyroid group (0.3 mg/bird/d) for 100 consecutive days; whereas the control group received drinking water only throughout the trial. At 64 weeks of age, 2 birds per replicate were killed by cervical dislocation and their oviducts were removed. For histomorphometrical observations, segments were taken from five different regions. After tissue preparation and staining with haematoxylin and eosin, histological layers were evaluated using light microscopy. The assessment of histomorphometrical characteristics of oviduct showed the height of mucosal folds in the magnum, thickness of mucosal folds of the magnum and uterus, thickness of tunica muscularis in the magnum and vagina, epithelial thickness of the isthmus and vagina, and uterine tubular glands percentage were decreased in the hyperthyroid birds compared with the control counterparts. The results showed long-term induced hyperthyroidism was associated with a decrease in a number of histomorphometrical traits in different regions of the oviduct. Some studies should be done to clarify to what extent the long-term maternal hyperthyroidism might affect the egg production, fertility rate, duration of fertility, and sperm penetration rate to make a final decision on exploitation of this preventative treatment to diminish the ascites incidence in progeny chicks.

Growth performance, survival, body composition, hematological parameters, intestinal histomorphology, and digestive enzymes’ activity in juvenile rainbow trout (Oncorhynchus mykiss) fed dietary Immunogen®

ABSTRACTThe aim of present study was to evaluate the effects of dietary Immunogen® supplementation on juvenile rainbow trout, where 120 fish (81.7 ± 2.23 g) were fed diets supplemented with either 0.0 (control) or 2 g Immunogen® kg−1 for 7 weeks. The fish fed the Immunogen®-supplemented diet showed an improvement in growth performance, feed utilization, and protein efficiency ratio and a decrease in feed conversion ratio. The fish fed the Immunogen®-supplemented diet showed higher body protein content. There were no significant differences in red blood cell count, eosinophil, or lymphocyte between the treatments. However, white blood cells, neutrophil, and monocyte were higher in the fish fed fish with the Immunogen®-supplemented diet. Supplementation of Immunogen® has increased villi height and tunica muscularis thickness as well as gut protease and lipase activities. The present study revealed that supplementation of Immunogen® can improve intestinal morphology and increase digestive enzyme activity and consequently increase trout growth and feed efficiency.

Histological Changes of Cervix in Ovariectomized Indigenous Rabbits

Histological changes which occurred in the cervix of the ovariectomized rabbits without hormone and with hormones injection were investigated.&#x0D; A total of 45 female rabbits were divided into three groups, the first group was used as a control, while, the second and third groups were used for experiments.&#x0D; In control group the tunica mucosa thrown into branched folds which lined with simple cuboidal to columnar epithelium, and a lamina propria was well vascular dense irregular collagenous connective tissue and a tunica muscularis displayed double layers of smooth muscle fibers. In ovariectomized group without hormones injection, the tunica mucosa showed delicate mucosal folds which lined with low simple cuboidal epithelium and the tunica muscularis was very thin layer, and a significant decreased in the height and thickness of mucosal folds, thickness of lamina propria and tunica muscularis. In ovariectomized groups with hormones injection, the cervix was very thick walled organ and their mucosal folds were the tallest, pyramidal shaped and their epithelium turned into simple columnar epithelium. The thickness of tunica muscularis was increased due to their two layers those intermingled with much of collagen bundles. Statistically, a significant (P&lt;0.05) increased in the values of all parameters.&#x0D; The present study concluded that that cervix in doe has a narrower lumen with huge collagenous connective tissue content that increased in response to steroid hormones, in addition to hyperplasia and hypertrophy, consequently for keeping intrauterine during the preimplantation stage and pregnancy.

Haemato-immunological and serum biochemical parameters, intestinal histomorphology and growth performance of rainbow trout (Oncorhynchus mykiss) fed dietary fermentable fibre (Vitacel®)

The aim of this study was to investigate the effects of dietary fermentable fibre (Vitacel®) on haemato-immunological and serum biochemical parameters, intestinal histomorphology, growth performance and proximate body composition of rainbow trout (Oncorhynchus mykiss). One hundred and twenty fish (81.65 ± 1.49 g) were fed diets containing 0 and 10 g kg−1 fermentable fibre (Vitacel®) for 50 days. At the end of feeding trial, growth performance parameters were significantly improved in fermentable fibre (Vitacel®)-fed fish. Although feeding on fermentable fibre (Vitacel®)-supplemented diet had no remarkable effect on haematological parameters (RBC, Htc and Hb) (P > 0.05), white blood cell (WBC) count and monocyte level were significantly affected (P = 0.040 and P = 0.020, respectively). In case of serum biochemical parameters, a significant increase and decrease were observed in serum total protein (P = 0.040) and cholesterol level (P = 0.000), respectively. Also, lysozyme level was significantly increased following administration of Vitacel® in diet (P = 0.006). Histomorphological evaluations revealed that villus length (P = 0.004) and tunica muscularis thickness (P = 0.000) were significantly higher in fermentable fibre (Vitacel®)-fed fish. These results indicate that fermentable fibre can be considered as a dietary supplement for improving the health status and performance of rainbow trout.

Influence of dietary fat sources and lysolecithin on growth performance, visceral organ size, and histological intestinal alteration in broiler chickens

The aim of this study was to investigate the influence of dietary fat sources and lysolecithin on growth performance, visceral organ size, and histological intestinal alterations in broiler chickens. Ninety-six 7-d-old male broiler chickens were assigned to 8 treatments in a 2×4 factorial arrangement of treatments with 2 levels of lysolecithin (0 and 145mg/kg) and 4 different fat sources [poultry fat (PF), yellow grease from Kagoshima (YG1), yellow grease from Kashima (YG2), and yellow grease from Hachinohe (YG3)]. Each treatment had 4 replicates of 3 broiler chickens per cage until 49d of age. There was no difference in BW gain due to fat sources, lysolecithin, or lysolecithin×fat source interaction throughout the experiment. By feeding lysolecithin, feed efficiency increased (P<0.05) during the growth period of 7 to 21d of age, and the weights of the duodenum, ileum, and total intestine decreased (P<0.05). Intestinal villus height, villus area, and cell area were not different among treatment groups, except that ileal villus height tended to decrease and ileal cell area tended to increase (P=0.077) by feeding lysolecithin. Increased duodenal cell mitosis and decreased jejunal tunica muscularis thickness were observed (P<0.05) by feeding lysolecithin. Furthermore, on the villus apical surface, more protuberated cells, cell clusters, and deeper cells at the sites of recently exfoliated cells were observed by feeding lysolecithin. However, the protuberant cells were not different among treatment groups. The Integrated Fluorescence Density Values of Anti-Cluster of Differentiation 36 reaction in the jejunum were significantly greater in the lysolecithin-fed groups, except YG2. These results indicate that feeding lysolecithin improves feed efficiency during 7 to 21d of age regardless of the fat type, and causes epithelial hypertrophy.