Thermal Traits Research Articles

Structural, optical, and thermal traits of Sm³⁺-doped SrB₂O₄ phosphors for solid-state lighting applications

We synthesized Sm³⁺-doped SrB₂O₄ phosphors through a solid-state reaction method, varying the Sm³⁺ doping concentrations. Structural and morphological characteristics of material were investigated using Fourier-transform infrared spectroscopy (FTIR), X-Ray Diffraction (XRD) and scanning electron microscopy (SEM). Optical transitions were analyzed by recording the diffuse reflectance spectroscopy, while photoluminescent (PL) spectra were used to evaluate luminescence properties. The PL spectra revealed a strong orange emission at 598 nm under 402 nm excitation. The optimal Sm³⁺ doping concentration was determined to be 0.02 mol, beyond which concentration quenching occurred. This quenching is attributed to exchange-type interactions, which facilitate non-radiative energy relaxation. The CIE color chromaticity coordinates of all the synthesized phosphors fell within the orange region of the chromaticity diagram. Temperature dependent photoluminescence revealed lower activation energy and phonon energy. Thermal quenching temperature was calculated which is inline with commercially available LEDs. All of these results indicate the candidature of SrB2O4 phosphor doped with Sm3+ ions for solid state lighting and optical thermal sensing applications.

Territorial status is explained by covariation between boldness, exploration, and thermal preference in a colour polymorphic lizard.

Colour polymorphic species often exhibit variation in morphology, physiology, and behaviour among morphs. In particular, dominance status may be signalled by the interaction between behaviour and colour morph. Behavioural traits associated with dominance include boldness, exploration, and aggression, which influence access to preferred habitat, territorial defence, and mate acquisition. In ectotherms, the social structure associated with morphs may result in the exploitation of structural niches differing in thermal quality. Hence, social interactions among morphs may generate concordant variation in thermal preference and environmental temperature. However, few studies have assessed thermal preference variation in colour polymorphic species and its covariation with behaviour. Doing so can provide insight into niche specialization and the maintenance of colour polymorphism in populations. Here, we investigated the patterns of covariation in boldness behaviour, exploratory behaviour, and thermal preference in the tree lizard, Urosaurus ornatus. We assessed trait variation between territorial and non-territorial male morphs and between orange and yellow female morphs. Boldness and exploratory behaviour were repeatable in male U. ornatus and bolder individuals were significantly more likely to incur tail loss, a potential consequence of bold behaviour. Territorial male morphs were significantly bolder and more exploratory and preferred higher body temperatures with a narrower T set than non-territorial morphs. Female morphs did not vary in behavioural or thermal traits. This study highlights behavioural mechanisms that underly ecological niche segregation and variable habitat use between morphs in a colour polymorphic species.

Ecotypic Variation in Leaf Thermoregulation and Heat Tolerance but Not Thermal Safety Margins in Tropical Trees.

To avoid reaching lethal temperatures during periods of heat stress, plants may acclimate either their biochemical thermal tolerance or leaf morphological and physiological characteristics to reduce leaf temperature (Tleaf). While plants from warmer environments may have a greater capacity to regulate Tleaf, the extent of intraspecific variation and contribution of provenance is relatively unexplored. We tested whether upland and lowland provenances of four tropical tree species grown in a common garden differed in their thermal safety margins by measuring leaf thermal traits, midday leaf-to-air temperature differences (∆Tleaf) and critical leaf temperatures defined by chlorophyll fluorescence (Tcrit). Provenance variation was species- and trait-specific. Higher ∆Tleaf and Tcrit were observed in the lowland provenance for Terminalia microcarpa, and in the upland provenance for Castanospermum australe, with no provenance effects in the other two species. Within-species covariation of Tcrit and ∆Tleaf led to a convergence of thermal safety margins across provenances. While future studies should expand the number of provenances and species investigated, our findings suggest that lowland and upland provenances may not differ substantially in their vulnerability to heat stress, as determined by thermal safety margins, despite differences in operating temperatures and Tcrit.

Nutrient availability influences the thermal response of marine diatoms

AbstractUnderstanding how phytoplankton growth responds to temperature is critical for forecasting marine productivity in a warming ocean. While previous laboratory studies have shown that phytoplankton thermal traits such as optimal temperature (Topt) can be affected by nutrient availability, it is unclear whether this can be extrapolated to natural communities. To address this, we tested the impacts of nutrient availability on the thermal responses of two cosmopolitan diatom genera, Pseudo‐nitzschia and Leptocylindrus, through a series of in situ manipulation experiments on natural phytoplankton communities. Analysis of the thermal performance curves revealed that nutrient limitation during summer not only limited the growth of these two genera but also reduced their Topt and the maximum growth rates (μmax). Topt was close to or lower than in situ temperature under ambient nutrient conditions, suggesting that further warming may have a detrimental effect on their growth. However, increasing nutrient supply could counteract this by enhancing Topt and μmax. To further confirm the interactive effects of nutrients and temperature on diatoms, we analyzed a 20‐yr monitoring dataset on Pseudo‐nitzschia, Leptocylindrus, and the whole diatom assembly in Hong Kong coastal waters. We found that the abundances of marine diatoms were significantly higher at high temperatures under nutrient‐rich environments while relatively low under low nutrient concentrations. Findings on natural diatom cell density align with the growth performance derived from in situ manipulation experiments, suggesting that abundant nutrients bolster marine diatoms in coping with warming. Our results highlight the importance of considering the influence of nutrient availability on thermal response of phytoplankton growth, which sheds light on how marine primary production may change under climate warming.

Alpine viper in changing climate: thermal ecology and prospects of a cold-adapted reptile in the warming Mediterranean

In a rapidly changing thermal environment, reptiles are primarily dependent on in situ adaptation because of their limited ability to disperse and the restricted opportunity to shift their ranges. However, the rapid pace of climate change may surpass these adaptation capabilities or elevate energy expenditures. Therefore, understanding the variability in thermal traits at both individual and population scales is crucial, offering insights into reptiles' vulnerability to climate change. We studied the thermal ecology of the endangered Greek meadow viper (Viperagraeca), an endemic venomous snake of fragmented alpine-subalpine meadows above 1600 m of the Pindos mountain range in Greece and Albania, to assess its susceptibility to anticipated changes in the alpine thermal environment. We measured preferred body temperature in artificial thermal gradient, field body temperatures of 74 individuals in five populations encompassing the entire geographic range of the species, and collected data on the available of temperatures for thermoregulation. We found that the preferred body temperature (Tp) differed only between the northernmost and the southernmost populations and increased with female body size but did not depend on sex or the gravidity status of females. Tp increased with latitude but was unaffected by the phylogenetic position of the populations. We also found high accuracy of thermoregulation in V.graeca populations and variation in the thermal quality of habitats throughout the range. The overall effectiveness of thermoregulation was high, indicating that V.graeca successfully achieves its target temperatures and exploits the thermal landscape. Current climatic conditions limit the activity period by an estimated 1278 h per year, which is expected to increase considerably under future climate scenarios. Restricted time available for thermoregulation, foraging and reproduction will represent a serious threat to the fitness of individuals and the persistence of populations in addition to habitat loss due to mining, tourism or skiing and habitat degradation due to overgrazing in the shrinking mountaintop habitats of V.graeca.

Drivers of Intraspecific Variation in Thermal Traits and Their Importance for Resilience to Global Change in Amphibians.

Intraspecific variation can be as great as variation across species, but the role of intraspecific variation in driving local and large-scale patterns is often overlooked, particularly in the field of thermal biology. In amphibians, which depend on environmental conditions and behavior to regulate body temperature, recognizing intraspecific thermal trait variation is essential to comprehensively understanding how global change impacts populations. Here, we examine the drivers of micro- and macrogeographical intraspecific thermal trait variation in amphibians. At the local scale, intraspecific variation can arise via changes in ontogeny, body size, and between the sexes, and developmental plasticity, acclimation, and maternal effects may modulate predictions of amphibian performance under future climate scenarios. At the macrogeographic scale, local adaptation in thermal traits may occur along latitudinal and elevational gradients, with seasonality and range-edge dynamics likely playing important roles in patterns that may impact future persistence. We also discuss the importance of considering disease as a factor affecting intraspecific variation in thermal traits and population resilience to climate change, given the impact of pathogens on thermal preferences and critical thermal limits of hosts. Finally, we make recommendations for future work in this area. Ultimately, our goal is to demonstrate why it is important for researchers to consider intraspecific variation to determine the resilience of amphibians to global change.

Larval diet impacts black soldier fly (Diptera: Stratiomyidae) thermal tolerance and preference.

Thermal tolerance and preference are key parameters impacting agricultural production systems. In this study, the impact of larval diet on black soldier fly thermal tolerance and preference across life-stages and sexes was examined. Larvae were fed either a low-protein high-carbohydrate synthetic diet (i.e., P7C35), a high-protein low-carbohydrate synthetic diet (i.e., P35C7), or the Gainesville diet (i.e., C) as a control and reference. Our results demonstrate that the impacts of larval diet on black soldier fly thermal tolerance and preference could be stage and sex specific. The mean heat knockdown temperatures (HKT) ranged between 46.6 and 47.9 °C. Synthetic diets resulted in greater HKT and the difference decreased form larvae (e.g., ∼1 °C) to adults (e.g., ∼0.2 °C). The mean chill-coma recovery time (CCRT) ranged between 8.3 and 21.6 min. Not much differences were detected between diets, but CCRT became longer from larvae to adults. The mean thermal preference ranged between 13.6 and 29.5 °C. Larvae fed synthetic diets preferred much lower temperatures than the control diet. A bimodal distribution was observed for adults regardless of sex. Differences on body mass, lipid, and protein contents were detected among diets; however, more research should be done before any conclusions can be linked to their thermal traits. These findings highlight the importance of considering the ingredients and nutritional makeup of larval diets when optimizing temperature management protocols for mass production of black soldier flies. Conversely, specific diets can be developed to promote survival under extreme rearing temperatures.

Insects in temperate urban parks face stronger selection pressure from the cold than the heat.

Urban areas experience higher temperatures compared to rural areas and as such, are increasingly considered places of acclimatization and adaptation to warming. Small ectotherms, such as insects, whose body temperature rises with habitat temperature, are directly affected by temperature changes. Thus, warming could have a profound effect on insect behavior and physiology. To test if the urban heat island effect drives higher thermal tolerance and activity changes, we used globally distributed and abundant insects-ants. We measured the heat and cold tolerance of 14 ant species distributed across urban and peri-urban areas. As thermal traits are often correlated with ant foraging, we measured foraging activity during three consecutive years across eight sites. Contrary to our prediction, ants exposed to the urban heat island effect did not have a higher heat tolerance than peri-urban ants. Instead, cold tolerance varied across habitats, with ants from the cooler, peri-urban habitats being able to tolerate lower temperatures. We recorded the same pattern of invariant heat and higher cold tolerance for ants in the canopy, compared to ground nesting ants. Ant activity was almost 10 times higher in urban sites and best predicted by cold, not heat tolerance. These unexpected results suggest that we need to rethink predictions about urban heat islands increasing insect heat tolerance in urban habitats, as cold tolerance might be a more plastic or adaptable trait, particularly in the temperate zone.

Evidence for an adult summer diapause in mountain pine beetle (Coleoptera: Curculionidae) that varies geographically and among haplogroups.

Identifying dormancy traits is important for predicting insect population success, particularly in a changing climate that could disrupt evolved traits. The mountain pine beetle (Dendroctonus ponderosae Hopkins) is native to North America, is responsible for millions of acres of tree mortality, and is expanding northward in Canada. Research has identified thermal traits important to epidemic-phase ecology that vary among populations. Genomic research identified 3 mountain pine beetle haplogroups representing Pleistocene glacial refugia. Significant variation in generation timing aligning with the haplogroups has been observed. The adult stage was previously identified as the likely cause of differences among populations, although the mechanism(s) remain unclear. We tested for an adult summer diapause that varies among populations from 2 haplogroups, southern Colorado (CO) (central haplogroup) and southern Idaho (ID) (eastern haplogroup) using respirometry and reproduction experiments. Warm temperatures (25 °C) resulted in reduced respiration rates of central haplogroup mountain pine beetle compared to a cool temperature treatment (15 °C), whereas respiration of the eastern haplogroup did not differ between the treatments. Mated pairs of central haplogroup mountain pine beetle reared/held at 15 °C were more likely to be classified with a higher reproductive success rating compared to pairs reared/held at 25 °C. These results support a facultative summer adult diapause in southern CO central haplogroup mountain pine beetle. Manifestation of this diapause was low/absent among adults from the northerly ID location. This diapause likely serves to maintain univoltinism shown to be important for mountain pine beetle epidemic-phase ecology. The variation occurring among haplogroups highlights the long-term, evolved processes driving local adaptations in mountain pine beetle.

Intraspecific variation in morphology and thermal ecology of the cleft lizard Sceloporus mucronatus (Squamata Phrynosomatidae): effect of habitat and sex

Populations of the same species that inhabit contrasting environments may be subject to different selection pressures, promoted by the local environment. Therefore, the individuals of the different populations respond to these pressures with adaptations to local environments; however, not all species or populations respond in the same way. We compared some morphological and ecological traits of the lizard Sceloporus mucronatus that inhabits contrasting environments in central Mexico. The results show male-biased sexual size dimorphism in Tecocomulco, but no differences in size between sexes in Parque Nacional El Chico (PNCH). Significant differences were observed in morphometric traits and in body mass between sexes and populations, but not in the interaction between these two factors. In general, females were heavier than males, while males had larger head structures, jaws, and hind limbs than females. In addition, differences were recorded in the volume of the abdomen, which was female-biased in the PNCH, and male-biased in Tecocomulco. Between populations, it was recorded that the organisms from the PNCH were heavier and had larger heads than those from Tecocomulco. Thermal traits showed differences among populations, but not between sexes nor in the interaction among these factors. Individuals from the locality with lower temperature and higher precipitation (PNCH) reached higher body temperatures and used warmer microhabitats than those individuals from the locality with higher temperature and lower precipitation (Tecocomulco). Females from both populations showed a greater degree of active thermoregulation compared to males. In addition, in both sexes and populations, average values of behavioral thermoregulation were different from zero. Significant differences were recorded in microhabitat use between populations. In agreement with previous studies of morphology and thermal ecology carried out in this species from this and other localities, and in different years, we suggest that its populations present a wide phenotypic plasticity.

Bring on the heat: thermal ecology of Tropidurus semitaeniatus in the Northeast of Brazil

Body temperature is a key physiological trait for individual fitness. Its effects on behavior and metabolism determine organismal activity. Optimal body temperature can vary among individuals, and consequent activity asynchrony can thus enable the co-occurrence of otherwise competing social strategies. Here, we combine ex-situ experiments with environmental data to test whether variability in thermal traits can explain the maintenance of the alternative reproductive strategies of Tropidurus semitaeniatus (Spix, 1825) male morphs. We predict that morphs and sexes differ in thermal traits. Thus, we tested for differences in their behaviorally determined thermal traits: preferred ( Tp), body ( Tb), and habitat temperatures; basking cover preference (Coverpref); and physiological limits (critical thermal minimum CTmin; and maximum, CTmax). We also calculated T. semitaeniatus’ thermal tolerance traits and compiled a list of Tp for Tropidurids. We found that T. semitaeniatus maintains its Tb below Tp. In addition, and contrary to theoretical predictions for tropical lizards, we found that T. semitaeniatus has a high warming tolerance (8.3 °C) and thermal safety margin (2.4 °C). We found no thermal differences between morphs indicating that thermal ecology does not play a role in the co-existence of color morphs.

Heat and mass transfer analysis for bi-dimensional bioconvective MHD nanofluid with varying thermal traits

ABSTRACT Bioconvection phenomena offer practical applications in biomicrosystems, fuel cells, biosensors, biocomputing, electronic thermal management, and renewable energy systems, showcasing their relevance and potential impact across various engineering sectors. Consequently, this study delves into the examination of a bi-dimensional magnetohydrodynamic nanofluid flow model encompassing the intricate dynamics of gyrotactic microorganisms, subject to the effects of suction and thermal radiation. The primary objective of the investigation is to optimize the thermal energy transfer efficiency of the fluid while concurrently minimizing associated costs, with the secondary aim of diminishing the frictional resistance at the fluid-solid interface. The basis for energy and momentum equations incorporating radiation using Rosseland’s approximation is established by the Buongiorno nanofluid model. The foundational PDEs along with their BCs, are reformulated into ODEs utilizing similarity variables, then transformed into a set of first-order ODEs ensuring compatibility with MATLAB’s bvp4c solver, which offers high convergence rates and accuracy due to its efficient algorithms and precision handling. Heat transfer rate boosts up by increasing suction and radiation parameters, while it dwindles due to an increase in the magnetic field. Enhancing suction and the magnetic field lowers skin friction. Microbial concentration surges by increasing the magnetic field, while suction minimizes it. Intensifying the thermophoresis parameter increases the concentration of nanoparticles.

Comparison of Hydric and Thermal Physiology in an Environmentally Diverse Clade of Caribbean Anoles.

As the world becomes warmer and precipitation patterns less predictable, organisms will experience greater heat and water stress. It is crucial to understand the factors that predict variation in thermal and hydric physiology among species. This study focuses on investigating the relationships between thermal and hydric diversity and their environmental predictors in a clade of Hispaniolan anole lizards, which are part of a broader Caribbean adaptive radiation. This clade, the "cybotoid" anoles, occupies a wide range of thermal habitats (from sea level to several kilometers above it) and hydric habitats (such as xeric scrub, broadleaf forest, and pine forest), setting up the possibility for ecophysiological specialization among species. Among the thermal traits, only cold tolerance is correlated with environmental temperature, and none of our climate variables are correlated with hydric physiology. Nevertheless, we found a negative relationship between heat tolerance (critical thermal maximum) and evaporative water loss at higher temperatures, such that more heat-tolerant lizards are also more desiccation-tolerant at higher temperatures. This finding hints at shared thermal and hydric specialization at higher temperatures, underscoring the importance of considering the interactive effects of temperature and water balance in ecophysiological studies. While ecophysiological differentiation is a core feature of the anole adaptive radiation, our results suggest that close relatives in this lineage do not diverge in hydric physiology and only diverge partially in thermal physiology.

Variation in thermal trait and plankton assemblage pattern induced by coal power plant discharge in river Ganga

Variation in thermal trait and plankton assemblage pattern induced by coal power plant discharge in river Ganga

Acclimation capacity to global warming of amphibians and freshwater fishes: Drivers, patterns, and data limitations.

Amphibians and fishes play a central role in shaping the structure and function of freshwater environments. These organisms have a limited capacity to disperse across different habitats and the thermal buffer offered by freshwater systems is small. Understanding determinants and patterns of their physiological sensitivity across life history is, therefore, imperative to predicting the impacts of climate change in freshwater systems. Based on a systematic literature review including 345 experiments with 998 estimates on 96 amphibian (Anura/Caudata) and 93 freshwater fish species (Teleostei), we conducted a quantitative synthesis to explore phylogenetic, ontogenetic, and biogeographic (thermal adaptation) patterns in upper thermal tolerance (CTmax) and thermal acclimation capacity (acclimation response ratio, ARR) as well as the influence of the methodology used to assess these thermal traits using a conditional inference tree analysis. We found globally consistent patterns in CTmax and ARR, with phylogeny (taxa/order), experimental methodology, climatic origin, and life stage as significant determinants of thermal traits. The analysis demonstrated that CTmax does not primarily depend on the climatic origin but on experimental acclimation temperature and duration, and life stage. Higher acclimation temperatures and longer acclimation times led to higher CTmax values, whereby Anuran larvae revealed a higher CTmax than older life stages. The ARR of freshwater fishes was more than twice that of amphibians. Differences in ARR between life stages were not significant. In addition to phylogenetic differences, we found that ARR also depended on acclimation duration, ramping rate, and adaptation to local temperature variability. However, the amount of data on early life stages is too small, methodologically inconsistent, and phylogenetically unbalanced to identify potential life cycle bottlenecks in thermal traits. We, therefore, propose methods to improve the robustness and comparability of CTmax/ARR data across species and life stages, which is crucial for the conservation of freshwater biodiversity under climate change.

Variability in the summer movements, habitat use and thermal biology of two fish species in a temperate river

The ability of fish to cope with warm water temperatures in summer depends on factors including their thermal traits and the ability of individuals to access cool-water refugia. Knowledge is highly limited on the in situ responses of many fishes to elevated summer temperatures, including whether they express behavioural thermoregulation. The responses of two riverine species to summer water temperatures were tested here using the movement metrics, spatial habitat use and body temperatures of individual European barbel Barbus barbus (‘barbel’) and common bream Abramis brama (‘bream’) versus river temperatures. Acoustic biotelemetry was applied in the lower River Severn basin, western Britain, in summer 2021 (barbel) and 2022 (bream), where individuals could move across > 150 km of river, including a tributary of cooler water. Across all individuals, bream occupied 37 km of river length (mainstem only), with low inter-individual variability in their spatial habitat use, movements and body temperatures. In contrast, barbel occupied 62 km of river (main river/tributary), with relatively high inter-individual variability in spatial habitat use, movements and body temperatures, with higher variation in body temperatures as river temperatures increased (maximum mean daily temperature difference between individuals on the same day: 4.2 °C). Although warmer individuals generally moved more, their activity was greatest at relatively low temperatures and higher flows, and neither species revealed any evidence of behavioural thermoregulation during elevated temperatures. Enabling phenotypically diverse fish populations to express their natural behaviours and thermal preferences in summer water temperatures thus requires maintaining their free-ranging in thermally heterogenous habitats.

Rural occupancy in a montane burrowing snake: the importance of thermal and microhabitat resources during the rainy season

Thermal and microhabitat resources are two fundamental requirements that affect the life history of any ectotherm. Furthering our understanding of how reptilian species meet these ecological requirements is crucial for assessing the impact of environmental and anthropogenic changes on populations. Here, we explored some fundamental aspects of the thermal and microhabitat ecology of Conopsis biserialis, a small, burrowing, and endemic earthsnake of central Mexico. The study was conducted during the rainy season in a montane site disturbed by rural activities. The mean field body temperature (Tb) of C. biserialis was 26 ± 0.6 ºC. Field Tb did not differ significantly between sex and development stages. However, we found that Tb was positively correlated with substrate, air, and under-rock temperatures (Ts, Ta, and Tr) in the rural microhabitat. Regression analysis showed that substrate and under-rock temperatures (Ts and Tr) were the parameters that best explained Tb variability in the individuals. The temperature and relative humidity under shelter did not differ significantly among shelter types and/or rock sizes. In addition to these thermal traits, earthsnakes selected and utilized six common biotic and physical elements of the rural microhabitat. Most individuals used high proportions of large and medium rocks for sheltering, and these rocks were selected around crop fences and cleared grasslands where the soil can be either covered with low vegetation or bare. Our results suggest that, during the rainy season, individuals of C. biserialis use and select some biotic and structural resources of the rural microhabitats, and were capable of actively regulating their temperature by using shelters with high values of microclimatic homogeneity.

Warming increases the compositional and functional variability of a temperate protist community

Phototrophic protists are a fundamental component of the world's oceans by serving as the primary source of energy, oxygen, and organic nutrients for the entire ecosystem. Due to the high thermal seasonality of their habitat, temperate protists could harbour many well-adapted species that tolerate ocean warming. However, these species may not sustain ecosystem functions equally well. To address these uncertainties, we conducted a 30-day mesocosm experiment to investigate how moderate (12 °C) and substantial (18 °C) warming compared to ambient conditions (6 °C) affect the composition (18S rRNA metabarcoding) and ecosystem functions (biomass, gross oxygen productivity, nutritional quality – C:N and C:P ratio) of a North Sea spring bloom community. Our results revealed warming-driven shifts in dominant protist groups, with haptophytes thriving at 12 °C and diatoms at 18 °C. Species responses primarily depended on the species' thermal traits, with indirect temperature effects on grazing being less relevant and phosphorus acting as a critical modulator. The species Phaeocystis globosa showed highest biomass on low phosphate concentrations and relatively increased in some replicates of both warming treatments. In line with this, the C:P ratio varied more with the presence of P. globosa than with temperature. Examining further ecosystem responses under warming, our study revealed lowered gross oxygen productivity but increased biomass accumulation whereas the C:N ratio remained unaltered. Although North Sea species exhibited resilience to elevated temperatures, a diminished functional similarity and heightened compositional variability indicate potential ecosystem repercussions for higher trophic levels. In conclusion, our research stresses the multifaceted nature of temperature effects on protist communities, emphasising the need for a holistic understanding that encompasses trait-based responses, indirect effects, and functional dynamics in the face of exacerbating temperature changes.

Thermal sensitivity of Rhinella arenarum tadpole at low concentrations of dimethoate pesticides

One of the main causes of contamination of aquatic environments, which affects biotic communities, is the use of pesticides in agricultural regions. Amphibians are considered good bio-indicators of aquatic pollution, because they are one of the most susceptible groups to pollution. Several studies suggest that both pollution and climate change produce synergistic effects in amphibians which amplify the toxicity afecting survival, and malformations with an increase in temperature. We studied the sensitivity of sublethal concentrations of dimethoate in Rhinella arenarum tadpoles on two fitness related thermal traits including locomotor swimming performance and thermal tolerance limits (CTmax = critical thermal maximum and CTmin = critical thermal minimum). The locomotor performance of R. arenarum tadpoles decreased with increasing sublethal dimethoate concentrations up to ∼60 % at intermediates dimethoate concentration. The tadpoles showed a tendency to decrease their tolerance to high temperatures (CTmax) with increasing dimethoate concentration around ∼0.5 °C, however no significant differences were found among treatments. Similarly, tadpoles showed decreases in their cold resistance (CTmin) with dimethoate concentrations, around 1 °C the high concentrations of dimethoate. The increase of atypical climatic events, such as heat waves may put R. arenarum tadpoles at greater risk when exposed to dimethoate. Our results show that the sublethal concentrations of the dimethoate pesticide may affect the fitness and survival of the larvae of R. arenarum in natural, and seminatural enviroments.

Population-specific responses to developmental temperature in the arboviral vector Aedes albopictus: Implications for climate change.

The increase of environmental temperature due to current global warming is not only favouring the expansion of the distribution range of many insect species, but it is also changing their phenology. Insect phenology is tightly linked to developmental timing, which is regulated by environmental temperatures. However, the degree to which the effects of developmental temperatures extend across developmental stages and their inter-stage relationships have not been thoroughly quantified in mosquitoes. Here, we used the mosquito Aedes albopictus, which is an aggressive invasive species and an arboviral vector, to study how developmental temperature influences fitness across developmental stages, thermal traits, energy reserves, transcriptome and Wolbachia prevalence in laboratory-reared populations originally collected from either temperate or tropical regions. We show that hatchability, larval and pupal viability and developmental speed are strongly influenced by temperature, and these effects extend to wing length, body mass, longevity and content of water, protein and lipids in adults in a population-specific manner. On the contrary, neither adult thermal preference nor heat resistance significantly change with temperature. Wolbachia density was generally lower in adult mosquitoes reared at 18°C than at other tested temperatures, and transcriptome analysis showed enrichment for functions linked to stress responses (i.e. cuticle proteins and chitin, cytochrome p450 and heat shock proteins) in mosquitoes reared at both 18 and 32°C. Our data showed an overall reduced vector fitness performance when mosquitoes were reared at 32°C, and the absence of isomorphy in the relationship between developmental stages and temperature in the laboratory population deriving from larvae collected in northern Italy. Altogether, these results have important implications for reliable model projections of the invasion potentials of Ae. albopictus and its epidemiological impact.