Thermal Cave Research Articles

Impacts of Different Habitats on the Composition of Bacterial Communities at the Discharging Endpoints of a Hypogene Thermal Karst System

The discharging zones of thermal karst caves provide special environmental conditions for bacteria involved in the biogeochemical processes. The taxonomic composition of bacterial communities inhabiting these still active natural habitats is largely unknown. The aim of this study was to reveal and compare the bacterial diversity of upwelling thermal waters, rock surface biofilms, and cave deposits using high-throughput next-generation sequencing. Two thermal karst caves formed in carbonate rocks and located at the discharging endpoints of the Transdanubian Range (Hungary) were studied: a sublacustrine spring cave and a phreatic hypogene cave. The results of 16S rRNA gene-based Illumina MiSeq amplicon sequencing revealed that almost all samples were dominated by Proteobacteria and/or Epsilonbacteraeota. Other abundant phyla showed habitat-dependent and diverse distributions. Sequences of the thermal waters were mainly associated with different chemolithotrophic sulfur-oxidizing bacteria (genera Thiovirga, Thiofaba, and Arcobacter in the sublacustrine thermal spring cave, whereas genera Sulfurovum, Sulfuricurvum, and Sulfurifustis were characteristic of the phreatic hypogene thermal cave) in accordance with the difference in environmental parameters. Sequences belonging to ammonia-oxidizing (unclassified Nitrosococcaceae) and nitrite-oxidizing bacteria (Nitrospira) were characteristic of the hypogene cave. Sequences related to photoautotrophic bacteria from the lake water and chemoheterotrophic bacteria from the sediment were only found in the sublacustrine spring cave samples.

Characterization of aerosol and assessment of the risk of transmission of SARS-COV-2 VIRUS in a natural thermal cave

Thermal caves represent an environment characterized by unique chemical-physical properties, often used by customers for treatment and care of musculoskeletal, respiratory and skin diseases. The recent pandemic caused by COVID-19 has imposed the need to investigate the potential transmission scenario of SARS-CoV-2 virus also in such atypical and poorly studied indoor environments. This research work was carried out inside a natural thermal cave located in Italy where a waterfall of sulfur-sulfate-bicarbonate-alkaline earth mineral thermal water creates a warm-humid environment with 100% humidity and 48°C temperature. A characterization of the aerosol was carried out in terms of number, surface area and mass, as well as particle size distributions. The physical characteristics of the aerosol were measured inside the natural thermal cave and in other immediately adjacent areas in two different days and in two distinct moments by means of an optical spectrometer. The data obtained showed a predominance of particles with a diameter greater than 8 μm, associated with a low ability of penetration in the human respiratory system. Subsequently, through a model recently proposed in scientific literature, it was evaluated the airborne transmission risk of SARS-CoV-2 inside the cave by quantifying the probability of infection due to exposure in a microenvironment in presence of a SARS-CoV-2 infected subject. The infection risk was evaluated for different scenarios obtained combining parameters such as physical, breathing or talking activities of the occupants, simultaneous or non-simultaneous access to the cave and mechanical ventilation activated or non-activated. In terms of the risk of SARS-CoV-2 infection, evaluated under the hypotheses of the model, it was highlighted the decisive effect of the mechanical ventilation system on the risk of contagion: for all the hypothesized scenarios, there is a substantial reduction in the risk of contagion considering the ventilation system active. Furthermore, the adoption of social distancing measures such as non-simultaneous access to the cave makes the risk of contagion extremely low, according to the assumptions underlying the model, even with the mechanical ventilation system not active.

Palaeokarst Deposits in Caves: Examples from Eastern Australia and Central Europe

Palaeokarst deposits are most commonly found in excavations, drill holes and naturally exposed at the Earth’s surface. Some caves however intersect palaeokarst deposits. This occurs in large hypogene caves in the USA, thermal caves in Hungary and in many caves in eastern Australia. Palaeokarst deposits in caves respond to cave forming processes in the same way as hostrock: the palaeokarst deposits form cave walls. A range of palaeokarst deposits is exposed in caves including; filled tubes, walls composed of flowstone, large-scale bodies, breccia pipes, dykes, volcaniclastic palaeokarst and crystalline palaeokarst. As well as being exposed in cave walls, palaeokarst deposits can wholly or partly form speleogens (speleogens made from palaeokarst). Records of geological events not preserved elsewhere can occur in palaeokarst deposits in caves. These can be difficult to correlate with conventional geological histories. It is important to be able to distinguish between palaeokarst deposits, relict sediments and phantom rock (in-situ weathered rock, also called ghost rock; Vergari & Quinif 1997). Relict sediments can be distinguished from palaeokarst deposits because the cave walls bound relict sediments while palaeokarst deposits form the cave walls. Palaeokarst can be distinguished from phantom rock, as palaeokarst is unconformable with the hostrock, with structures in the hostrock not continuing across the boundary into the palaeokarst. Hostrock structures and textures do continue across the boundary between unaltered hostrock and phantom rock. Similarly, cave sediments are unconformable or disconformable with the hostrock while phantom rock is conformable with hostrock containing hostrock structures and textures. It has been difficult to explain why palaeokarst occurs in some caves and not others. One explanation worth considering is that palaeokarst deposits are not intersected by caves or sections of caves that contain large perennial streams and/or have undergone large-scale vadose fluvial development capable of escaping from the bounds of structural guidance, such as the caves in the Classical Karst. Key words: palaeokarst, caves, Australia, relict sediments, phantom rock.

SPELEOTHERAPY DEVELOPMENT IN ROMANIA ON THE WORLD CONTEXT AND PERSPECTIVES FOR USE OF SOME SALT MINES AND KARST CAVES FOR SPELEOTHERAPEUTIC AND BALNEOCLIMATIC TOURISM PURPOSES

Speleotherapy (ST) is a relatively new method of complementary medicine, although, as is well known ancient tribes appreciated isolation in underwater caves, salt or karst caves, or possessing various ores and was officially recognized in the years 1950-1960 in Germany (K.Spannagel, 1961) and Poland (M. Skulimowski, 1965). Professor Mieczyslaw Skulimowski has granted of speleotherapy in Wieliczka Salt Mine, the name of subterraneotherapy, soon to be also called the Skulimowski method. Number of speleotherapeutic centers and speleotherapy symposiums has increased considerably (H.Trimmel, 1994). Speleotherapy in the underground now is an effective therapeutic method for the treatment of obstructive respiratory tract diseases, especially of patients with bronchial asthma (BA), the upward and effective practice in Central and Eastern Europe, but also in the West: Austria, Czech Republic, Germany, Poland, Russia, Slovakia, Ukraine, Belarus, Hungary etc. In the World, known about 60 underground salt and other mines, carst caves used for ST or are under experimental study to evaluate the possibility to be used for speleotherapy, including: Germany, Hungary, Czech Republic, Slovakia, Austria, Slovenia, Ukraine, Romania and other countries. In salt mines with 9-24 °C temperature operating 15 speleotherapy centers, 34 in karst caves with 9-18 °C, and 4 centers in thermal caves with temperatures higher than 24 C (Iu. Simionca et al., 2005). In the last twenty years ST occupies an important place in salt mines mines and carst caves with different parameters: Wieliczka, Poland; Solotvino / Slatina, Ukraine; Bad Bleiberg, Austria; Klutert Hohl, Germany; Zlate Hory and Cisarska Cave, Czech Republic; Bystrianska Jaskyna, Slovakia; Tapolca Seehohle, Hungary; Unirea Salt Mine Slanic Prahova, Romania and will occupy a significant place the Turda Salt Mine, Cacica, Tg. Ocna, and Praid salt mines from Romania (Iu.Simionca et al., 2006, 2009). Is of scientific and practical interest formation in world of new directions in the treatment of the most common and severe allergic diseases asthma, namely the use of underground environmental factors in order to reduce human outcome of the action of environmental allergy. In this context, the use of salt mines and caves as natural therapeutic factors also known as speleotherapy, is a special scientific interest, a perspective direction in the areas of health and environment. Today speleotherapy is known as the therapy of patients with various diseases in the underground salt mines and caves possessing natural therapeutic factors (Iu.Simionca et al., 1998, 2005, 2006, 2009). Analysis of the speleotherapy domain status allowed to establish that use saline or cave underground natural environmental factors which own curative properties for prevention, treatment and rehabilitation of patients with certain diseases, often

The Role of Condensation Corrosion in Thermal Speleogenesis. Study of a Hypogenic Sulfidic cCve in Aix-les-Bains, France

Condensation-corrosion is an active speleogenetical process in thermal caves where high thermal gradient drives air convection. Wall retreat rates are greater than in meteoric caves. Conversely, evaporation produces depositional processes by replacement of limestone by gypsum and by aerosol decantation leading to the formation of popcorns. The Chevalley Aven belongs to Aix-les-Bains thermal-sulfidic cave system. Condensation occurs at the contact of cool walls of large spheres; conversely, evaporation occurs at the output of the narrow passages where the air sinks down from the upper sphere. A weathered layer and biofilms are present where slow condensation occurs. Corrosion distribution varies according to thermal rock conductivity and causes the sphere to develop upwards, laterally, and divergent. This mor­phodynamic pattern favors the development of stacked spheres, isolated by narrow necks, and arranged in a bush-like pattern. This development is clearly active in the vadose zone above the thermal water table. We propose that some avens above wa­ter table hypogenic caves, like Villa Luz (Mexico), may be of condensation-corrosion origin instead of phreatic. Future de­velopment will collect physical and chemical data to calculate the condensation-corrosion budget and assess its role in cave development