Thermal Adaptation Research Articles

The impact of heatwaves on human perceived thermal comfort and thermal resilience potential in urban public open spaces

Climate change increases the likelihood of heatwave events, causing human thermal discomfort and even mortality. However, it is not clear to what extent humans with long-term and short-term experience of hot-summer exposure can adapt to thermal comfort in urban public open spaces when both experience their heatwave periods. Therefore, this study aims to investigate outdoor perceived thermal comfort in urban public open spaces during heatwave periods between two groups of people who have long-term and short-term experience of hot-summer exposure. Field surveys were conducted in public squares and parks during the heatwaves in Chongqing, China and Reading, the UK. Chongqing is known as a ‘furnace city’ and people have been living in a hot summer for a long time, while in Reading the summer is warm and people unusually experience the heatwave. The main results show that Chongqing respondents living in a hot climate for a longer period can endure more heat than Reading respondents during the heatwaves, indicating that Chongqing respondents have more thermal resilience. Besides, different behavioural adaptation measures show that people are active participants to choose their thermal preferences, rather than passive recipients of the thermal environments. The research implication contributes that protective measures against heatwaves need to be taken for pedestrians, including more shaded places with efficient ventilation design for sheltering and handy facilities such as drinking fountains and water spray. The research has novelty in deepening the dynamic theory of human thermal comfort and providing empirical evidence of thermal adaptation in extreme-high temperature events.

Comparative experimental evolution reveals species-specific idiosyncrasies in marine phytoplankton adaptation to warming.

A number of experimental studies have demonstrated that phytoplankton can display rapid thermal adaptation in response to warmed environments. While these studies provide insight into the evolutionary responses of single species, they tend to employ different experimental techniques. Consequently, our ability to compare the potential for thermal adaptation across different, ecologically relevant, species remains limited. Here, we address this limitation by conducting simultaneous long-term warming experiments with the same experimental design on clonal isolates of three phylogenetically diverse species of marine phytoplankton; the cyanobacterium Synechococcus sp., the prasinophyte Ostreococcus tauri and the diatom Phaeodoactylum tricornutum. Over the same experimental time period, we observed differing levels of thermal adaptation in response to stressful supra-optimal temperatures. Synechococcus sp. displayed the greatest improvement in fitness (i.e., growth rate) and thermal tolerance (i.e., temperature limits of growth). Ostreococcus tauri was able to improve fitness and thermal tolerance, but to a lesser extent. Finally, Phaeodoactylum tricornutum showed no signs of adaptation. These findings could help us understand how the structure of phytoplankton communities may change in response to warming, and possible biogeochemical implications, as some species show relatively more rapid adaptive shifts in their thermal tolerance.

Ribosomal proteins can hold a more accurate record of bacterial thermal adaptation compared to rRNA.

Ribosomal genes are widely used as 'molecular clocks' to infer evolutionary relationships between species. However, their utility as 'molecular thermometers' for estimating optimal growth temperature of microorganisms remains uncertain. Previously, some estimations were made using the nucleotide composition of ribosomal RNA (rRNA), but the universal application of this approach was hindered by numerous outliers. In this study, we aimed to address this problem by identifying additional indicators of thermal adaptation within the sequences of ribosomal proteins. By comparing sequences from 2021 bacteria with known optimal growth temperature, we identified novel indicators among the metal-binding residues of ribosomal proteins. We found that these residues serve as conserved adaptive features for bacteria thriving above 40°C, but not at lower temperatures. Furthermore, the presence of these metal-binding residues exhibited a stronger correlation with the optimal growth temperature of bacteria compared to the commonly used correlation with the 16S rRNA GC content. And an even more accurate correlation was observed between the optimal growth temperature and the YVIWREL amino acid content within ribosomal proteins. Overall, our work suggests that ribosomal proteins contain a more accurate record of bacterial thermal adaptation compared to rRNA. This finding may simplify the analysis of unculturable and extinct species.

Impact of North Indian Atmospheric Diabatic Heating on Summer Precipitation in Central Asia

Abstract The impact of North Indian atmospheric diabatic heating variation on summer rainfall over Central Asia (CA) at an interannual scale during 1960–2019 was investigated from thermal adaptation and water vapor transportation perspective. The results showed that more precipitation in southeastern CA is associated with the southward subtropical westerly jet (SWJ), caused by the ascending motion and weakened water vapor output on the south side. When the SWJ moves southward, the high-level water vapor transportation on the south side changes from outward (−1.9 × 106 kg s−1) to inward (0.6 × 106 kg s−1), and the positive anomalous relative vorticity advections by the basic westerly winds produce ascending anomalies over southeastern CA. The position change in the SWJ was mainly related to atmospheric diabatic heating over northern India (NI). The thermal vorticity adaptation caused by a weakened heating rate over NI leads to an anomalous upper-level cyclone over southeastern CA, and the associated cold temperature advection eventually cools the upper troposphere of southeastern CA and reduces the temperature gradient at mid-to-high latitudes, leading to the southward SWJ. Thermal adaptation of the circulation and temperature anomaly over southeastern CA to the NI thermal forcing were also verified by numerical experiments. Both the abnormal ascending motions and the weakened outward water vapor associated with the southward SWJ, caused by the weakened heating rate over NI, lead to more summer rainfall in southeastern CA. The changes in diabatic heating over NI are closely related to Indian Ocean SST. When the Indian Ocean SST is warmer, the south Asian summer monsoon weakens, causing less precipitation and, thus, a weakened heating rate over NI. Significance Statement This study established that the northern Indian atmospheric diabatic heating anomalies associated with Indian Ocean SST variation play an important role in influencing precipitation in central Asia (CA). The weakening of the atmospheric diabatic heating over the NI would not only cause an abnormal cyclone and cooling over southern CA through thermal adaptation but also lead to southward subtropical westerly jet (SWJ), ascending motions, and decreased outward water vapor on the south side in southeastern CA, eventually resulting in more precipitation in southeastern CA. The results emphasize the influence of tropical SST and atmospheric heat sources on midlatitude climate and are important for understanding summer precipitation change in southeastern CA.

The Adaptive Value of Chromosomal Inversions and Climatic Change-Studies on the Natural Populations of Drosophila subobscura from the Balkans.

The adaptive value of the Drosophila subobscura chromosomal inversion polymorphism with regard to environmental effects is well-known. However, the specific details of the inversion adaptations to the global warming scenario deserve to be analyzed. Toward this aim, polymorphism and karyotypes were studied in 574 individuals from Petnica (Serbia) in annual samples taken in June for the period 2019-2022. Comparing the results of Petnica (Cfa: humid subtropical climate) with those from Avala (Serbia: Cfb, temperate oceanic climate) and Font Groga (Barcelona, Spain; Csa: hot-summer Mediterranean climate), significant differences were observed for their chromosomal polymorphism. In Petnica, inversions from U and E chromosomes mainly reacted significantly with regard to temperature, humidity, and rainfall. Moreover, the inversion polymorphism from Petnica (2019-2022) was compared with that from 1995. In this period, a significant increase in mean and maximum temperature was observed. However, to properly explain the observed variations of inversions over time, it was necessary to carefully analyze annual seasonal changes and particular heat wave episodes. Interestingly, yearly fluctuations of U chromosome 'warm'-adapted inversions corresponded with opposite changes in 'non-thermal' inversions. Perhaps these types of inversions were not correctly defined with regard to thermal adaptation, or these fluctuations were also due to adaptations to other physical and/or biological variables. Finally, a joint study of chromosomal inversion polymorphism from many Balkan populations of D. subobscura indicated that different climatic regions presented distinct composition, including thermal-adapted inversions.

The two chytrid pathogens of amphibians in Eurasia—climatic niches and future expansion

BackgroundClimate affects the thermal adaptation and distribution of hosts, and drives the spread of Chytridiomycosis—a keratin-associated infectious disease of amphibians caused by the sister pathogens Batrachochytrium dendrobatidi (Bd) and B. salamandrivorans (Bsal). We focus on their climate-pathogen relationships in Eurasia, the only region where their geographical distributions overlap. Eurasia harbours invaded and native areas of both pathogens and the natural habitats where they co-exist, making it an ideal region to examine their environmental niche correlations. Our understanding of how climate change will affect their distribution is broadened by the differences in climate correlates and niche characteristics between Bd and Bsal in Asia and Europe. This knowledge has potential conservation implications, informing future spread of the disease in different regions.ResultsWe quantified the environmental niche overlap between Bd and Bsal in Eurasia using niche analyses. Results revealed partial overlap in the niche with a unique 4% of non-overlapping values for Bsal, suggesting segregation along certain climate axes. Bd tolerates higher temperature fluctuations, while Bsal requires more stable, lower temperature and wetter conditions. Projections of their Realized Climatic Niches (RCNs) to future conditions show a larger expansion of suitable ranges (SRs) for Bd compared to Bsal in both Asia and Europe, with their centroids shifting in different directions. Notably, both pathogens' highly suitable areas in Asia are expected to shrink significantly, especially under the extreme climate scenarios. In Europe, they are expected to expand significantly.ConclusionsClimate change will impact or increase disease risk to amphibian hosts, particularly in Europe. Given the shared niche space of the two pathogens across available climate gradients, as has already been witnessed in Eurasia with an increased range expansion and niche overlap due to climate change, we expect that regions where Bsal is currently absent but salamanders are present, and where Bd is already prevalent, may be conducive for the spread of Bsal.

Hybrid ball-hinged secondary mirror assembly for high-precision surface shape maintenance.

As an essential part of optical telescope, the secondary mirror is subject to the influence of ambient temperature, which leads to temperature-induced distortion on the surface shape. A hybrid ball-hinged secondary mirror assembly (HSMA) is proposed to achieve thermal adaptation over a wide range of temperature. Simulation investigation on the temperature-induced surface shape distortion of the HSMA were carried out by using the finite element model. Simulation results show that the change of secondary mirror surface distortions over a wide range of temperature are minimal and negligible. For the wide ambient temperature range from -30°C to 70°C, the PV and RMS values of the maximum residual distortions can reach as small as 16.31 nm and 3.005 nm, respectively. Furthermore, the influence of gravity-induced distortion on the surface shape is also carried out. Both simulation and experiment results show that the HSMA is able to maintain high-precision surface shape of the secondary mirror over a wide range of temperature and at different attitudes from 0 to 90 ∘.

Population divergence in maternal investment and embryo energy use and allocation suggests adaptive responses to cool climates.

The thermal sensitivity of early life stages can play a fundamental role in constraining species distributions. For egg-laying ectotherms, cool temperatures often extend development time and exacerbate developmental energy cost. Despite these costs, egg laying is still observed at high latitudes and altitudes. How embryos overcome the developmental constraints posed by cool climates is crucial knowledge for explaining the persistence of oviparous species in such environments and for understanding thermal adaptation more broadly. Here, we studied maternal investment and embryo energy use and allocation in wall lizards spanning altitudinal regions, as potential mechanisms that enable successful development to hatching in cool climates. Specifically, we compared population-level differences in (1) investment from mothers (egg mass, embryo retention and thyroid yolk hormone concentration), (2) embryo energy expenditure during development, and (3) embryo energy allocation from yolk towards tissue. We found evidence that energy expenditure was greater under cool compared with warm incubation temperatures. Females from relatively cool regions did not compensate for this energetic cost of development by producing larger eggs or increasing thyroid hormone concentration in yolk. Instead, embryos from the high-altitude region used less energy to complete development, that is, they developed faster without a concomitant increase in metabolic rate, compared with those from the low-altitude region. Embryos from high altitudes also allocated relatively more energy towards tissue production, hatching with lower residual yolk: tissue ratios than low-altitude region embryos. These results are consistent with local adaptation to cool climate and suggest that this is underpinned by mechanisms that regulate embryonic utilisation of yolk reserves and its allocation towards tissue, rather than shifts in maternal investment of yolk content or composition.

Obg plays a significant role in temperature sensitivity of Mycoplasma synoviae live attenuated vaccine strain MS-H

The MS-H vaccine strain (Vaxsafe MS®; Bioproperties Pty. Ltd., Australia) is a live attenuated temperature sensitive derivative of a virulent strain of M. synoviae, 86079/7NS, and is used to prevent diseases from M. synoviae challenges in poultry farms. The genome sequence of MS-H includes 32 single nucleotide polymorphisms (SNPs) compared to that of 86079/7NS. To investigate the nature of mutations responsible for temperature sensitivity, MS-H strain was subjected to thermal adaptation in vitro and in vivo. The only observed variation detected in the MS-H culture following sequential passages with incremental incubation temperature from 33 °C to 39.5 °C was an Ala210Val variation in Obg protein, associated with loss of temperature sensitivity phenotype. An identical variation was detected in the MS-H culture reisolated from one out of five bird 28 days after inoculation with MS-H. These findings suggest that M. synoviae is capable of thermoadaptive evolution and Obg plays a significant role in this trait.

Western pine beetle voltinism in a changing California climate

Abstract Recent hot droughts in California resulted in ponderosa pine (Pinus ponderosa) mortality attributed to drought and western pine beetle (WPB, Dendroctonus brevicomis). While drought alone can cause tree death, direct warming effects on WPB are a contributing factor. Research on WPB generation timing (voltinism), however, is lacking. We monitored WPB tree attacks and adult emergence timing at two California sites and developed a degree‐day model from field‐observed data. Historical, contemporary, and future temperatures for several California sites were used with the model to examine trends in WPB voltinism. Field data showed a single summer and an overwinter generation at a northern California site. As summer temperatures increased beyond 1900–1980 averages, the predicted number of full and partial WPB generations by 2021 had increased from ~2 annual (one summer and one overwinter) generations historically to ~2.3 at two northern California sites and from ~2.3 to ~3.2 at two warmer California sites. Historical and contemporary data suggest winter warming was not sufficient for an additional generation overwinter. Instead, increases in generations were driven by summer and fall temperatures. Unconstrained increases in the number of future annual generations will be limited by complex, but not well understood, WPB thermal adaptations. Increased knowledge of temperature‐driven WPB population growth will improve forest vegetation models aimed at predicting ponderosa pine mortality in a changing climate.

Slow and population specific evolutionary response to a warming environment

Adaptation to increasingly warmer environments may be critical to avoid extinction. Whether and how these adaptive responses can arise is under debate. Though several studies have tackled evolutionary responses under different thermal selective regimes, very few have specifically addressed the underlying patterns of thermal adaptation under scenarios of progressive warming conditions. Also, considering how much past history affects such evolutionary response is critical. Here, we report a long-term experimental evolution study addressing the adaptive response of Drosophila subobscura populations with distinct biogeographical history to two thermal regimes. Our results showed clear differences between the historically differentiated populations, with adaptation to the warming conditions only evident in the low latitude populations. Furthermore, this adaptation was only detected after more than 30 generations of thermal evolution. Our findings show some evolutionary potential of Drosophila populations to respond to a warming environment, but the response was slow and population specific, emphasizing limitations to the ability of ectotherms to adapt to rapid thermal shifts.

Chromosome-level genome and population genomics provide novel insights into adaptive divergence in allopatric Eleutheronema tetradactylum

Understanding the adaptive ecological divergence provides important information for revealing biodiversity generation and maintenance. Adaptive ecology divergence in populations occurs in various environments and locations, but its genetic underpinnings remain elusive. We generated a chromosome-level genome of Eleutheronema tetradactylum (~582 Mb) and re-sequenced 50 allopatric E. tetradactylum in two independent environmental axes in China and Thailand Coastal waters as well as 11 cultured relatives. A low level of whole genome-wide diversity explained their decreased adaptive potential in the wild environment. Demographic analysis showed evidence of historically high abundance followed by a continuous distinct decline, plus signs of recent inbreeding and accumulation of deleterious mutations. Extensive signals of selective sweeps with signs of local adaptation to environmental differentiation between China and Thailand at genes related to thermal and salinity adaptation were discovered, which might be the driving factors of the geographical divergence of E. tetradactylum. Many genes and pathways subjected to strong selection under artificial breeding were associated with fatty acids and immunity (ELOVL6L, MAPK, p53/NF-kB), likely contributing to the eventual adaptation of artificial selective breeding. Our comprehensive study provided crucial genetic information for E. tetradactylum, with implications for the further conservation efforts of this threatened and ecologically valuable fish.

Investigating the effects of climate on thermal adaptation: A comparative field study in naturally ventilated university classrooms

In educational buildings, adaptive strategies can be adopted for the achievement of thermal comfort and reduction of energy consumption. Since climate can largely affect thermal comfort, there is a need for understanding its role in the definition of different adaptive capacities, thermal neutrality, comfort, and preference.To this end, 17 naturally ventilated university classrooms from 10 different buildings located in two sub-climates of Italy (Mediterranean climate) and France (Continental climate) were analysed. In total, 1377 questionnaires associated with environmental parameters were collected. The same educational stage (i.e. university classrooms) and operation mode (i.e. naturally ventilated during the heating period) were investigated to remove possible biases related to their influence on thermal comfort perception.Field studies show that despite French students performing less adaptive actions, their neutral temperature (TN) was 3.1 °C lower than the Italian ones (TN,ITALY = 23.6 °C and TN,FRANCE = 20.5 °C) and this difference was statistically significant.Adaptation as a function of the sub-climate was evident from the comparison with the PMV-PPD model. Neutral temperatures calculated with PMV were higher than those obtained from TSV, and the difference increased for the French colder climate.Practically, students’ adaptation to colder environments can be deployed to ensure comfort while reducing the heating demand.

Experimental study on soot formation and primary particle size in oxy-combustion ethylene diffusion flames under CO2 substitution for N2

This paper reports an experimental study on the effects of O2 concentration and CO2 concentration on soot volume fraction and primary particle size of flame centre in ethylene laminar co-diffusion flame. The incandescent light was induced by a two-color band method and laser-excited by wavelength 1064 nm. Previous studies have shown that the oxygen concentration affects the soot volume fraction in the flame. However, the effects of O2 concentration on the primary particle size under CO2 atmosphere are unclear and need to be further discussed. Seven flames were optically detected, in which the oxygen concentration of the accompanying gas varied from 21% to 50% by volume. One primary flame was burned in co-flowing ethylene/air. Six ethylene flames were burned in an oxygen-rich O2/N2 or O2/CO2 atmosphere with an oxygen mole fraction from 30% to 50%. The C2H4 flow rate remained constant in all operating conditions. The results show that the SVF observed by the LII method and spectroscopy in the oxygen-enriched flame are in good agreement. Furthermore, the soot's primary particle diameter measured by the time-resolved LII method is in good agreement with the transmission electron microscope image analysis.With the increase of oxygen concentration, the flame height becomes shorter, the luminosity becomes brighter, and the soot yield increases. Compared with an N2 atmosphere with the same oxygen concentration, the soot yield in a CO2 atmosphere was significantly inhibited. In all the flames studied, the SVF along the flame axis showed a similar distribution: it first increased to the maximum value and then decreased rapidly until the flame tip. The maximum SVF on the axis appears between 0.64 and 0.78 based on the normalized height of the visible flame. In all ethylene/(O2/N2) flames, the maximum primary particle diameter and average primary particle diameter increase with oxygen content. For ethylene/(O2/CO2) flame, the maximum diameter of primary particles decreases with the increased oxygen content. The uncertainty of primary diameter is mainly caused by the thermal adaptation coefficient, the lower LII signal of smaller particles and the uncertainty of local flame temperature.

The role of body mass in limiting post heat-coma recovery ability in terrestrial ectotherms.

Under global warming, animal species show shrinking body size responses, cascading deep changes in community structure and ecosystem functions. Although the exact physiological mechanisms behind this phenomenon remain unsolved, smaller individuals may benefit from warming climate more than larger ones. Heat-coma, a physiological state with severe consequences on locomotion ability, is often considered as an "ecological death" scenario under which individuals are unable to escape and exposed to predation, further heat injury, and other hazards. Species are expected to increasingly encounter heat-coma temperature thresholds under warming climate, and body size may be an important trait for thermoregulation in particular for ectotherms. The relationship between heat-coma and shrinking body size remains, however, unclear. Yet, recovery after short-term heat-coma is possible, but little is known about its importance in thermal adaptation and how organismal size correlates with post heat-coma recovery. Here, using ants as a model system, we firstly examined the fate of heat-comatose individuals under field conditions to quantify the ecological benefits of post heat-coma recovery. Then, we quantified ants' recovery ability after heat-coma using a dynamic thermal assay in the laboratory and asked if thermal resilience varies between species with different body mass. Our results confirm that heat-coma represents an inherent ecological death where individuals failed to recover from coma suffer strong predation pressure. Additionally, following phylogenetic signals inclusion, organisms with small mass were more likely to recover, supporting the temperature-size rule in thermal adaptation and recent studies showing a decrease in body size composition of ectotherm community under warmer climatic conditions. Body size as a fundamental trait in ecology thus affects ectotherm survival under thermal stress, which may drive species body size adaptations and community composition under future warming scenarios.

Individual Factors that Affect the Perceived Thermal Comfort Condition during Heating Seasons

This paper investigated the effect of individual differences and the type of heating system on the perceived thermal comfort condition, neutral temperature, and thermal sensitivity in cold outdoor air conditions. Three types of individual factors, such as gender, temperament, and thermal past experience, were studied and compared in this paper. According to the results, temperament and thermal past experience directly influenced the occupants’ thermal preference and indirectly influenced the thermal sensation by affecting their clothing level. However, the effect size of such variations was negligible. The type of heating system had the most significant impact on the neutral temperature, with a mean difference of 2.8°C. Individual factors did not have a direct impact on the neutral temperature. However, thermal sensitivity was influenced by an individual’s temperament. Occupants with a cold temperament were more sensitive to the variation in the running mean outdoor temperature (Trm) than those with a warm temperament. When the results were compared with the predictions by the adaptive thermal heat balance (ATHB) and the predicted mean vote (PMV) models, it indicated that if the control condition over the heating system became limited, there would be no (i.e., very low) behavioral, physiological, and psychological adaptation. However, by creating an opportunity to control the heating system, thermal adaptation could be reached at a lower temperature under cold outdoor air conditions.

Loss of mitochondrial performance at high temperatures is correlated with upper thermal tolerance among populations of an intertidal copepod.

Environmental temperatures have pervasive effects on the performance and tolerance of ectothermic organisms, and thermal tolerance limits likely play key roles underlying biogeographic ranges and responses to environmental change. Mitochondria are central to metabolic processes in eukaryotic cells, and these metabolic functions are thermally sensitive; however, potential relationships between mitochondrial function, thermal tolerance limits and local thermal adaptation in general remain unresolved. Loss of ATP synthesis capacity at high temperatures has recently been suggested as a mechanistic link between mitochondrial function and upper thermal tolerance limits. Here we use a common-garden experiment with seven locally adapted populations of intertidal copepods (Tigriopus californicus), spanning approximately 21.5° latitude, to assess genetically based variation in the thermal performance curves of maximal ATP synthesis rates in isolated mitochondria. These thermal performance curves displayed substantial variation among populations with higher ATP synthesis rates at lower temperatures (20-25°C) in northern populations than in southern populations. In contrast, mitochondria from southern populations maintained ATP synthesis rates at higher temperatures than the temperatures that caused loss of ATP synthesis capacity in mitochondria from northern populations. Additionally, there was a tight correlation between the thermal limits of ATP synthesis and previously determined variation in upper thermal tolerance limits among populations. This suggests that mitochondria may play an important role in latitudinal thermal adaptation in T. californicus, and supports the hypothesis that loss of mitochondrial performance at high temperatures is linked to whole-organism thermal tolerance limits in this ectotherm.

Biomass-Derived Bifunctional Cathode Electrocatalyst and Multiadaptive Gel Electrolyte for High-Performance Flexible Zn-Air Batteries in Wide Temperature Range.

High-efficiency and low-cost bifunctional electrocatalysts for oxygen reduction reaction (ORR) and oxygen evolution reaction (OER), as well as gel electrolytes with high thermal and mechanical adaptability are required for the development of flexible batteries. Herein, abundant Setaria Viridis (SV) biomass is selected as the precursor to prepare porous N-doped carbon tubes with high specific surface area and the 900°C calcination product of SV (SV-900) shows the optimum ORR/OER activities with a small EOER -EORR of 0.734 V. Meanwhile, a new multifunctional gel electrolyte named C20E2G5 is prepared using cellulose extracted from another widely distributed biomass named flax as the skeleton, epichlorohydrin as the cross-linker and glycerol as the antifreezing agent. C20E2G5 possesses high ionic conductivity from -40 to + 60 °C, excellent tensile and compressive resistance, high adhesion, strong freezing and heat resistance. Moreover, the symmetrical cell assembled with C20E2G5 can significantly inhibit Zn dendrite growth. Finally, flexible solid-state Zn-air batteries assembled with SV-900 and C20E2G5 show high open circuit voltage, large energy density, and long-term operation stability between -40 and + 60°C. This biomass-based approach is generic and can be used for the development of diverse next-generation electrochemical energy conversion and storage devices.

A very long-chain fatty acid enzyme gene, PxHacd2 affects the temperature adaptability of a cosmopolitan insect by altering epidermal permeability

Temperature fluctuations pose challenges to poikilotherms, such as insects, especially under climate change conditions. Very long-chain fatty acids (VLCFAs) form important structural components of membranes and epidermal surfaces, so play important roles in adaptation to temperature stress in plants. It has been unclear whether VLCFAs are involved in epidermis formation and thermal resistance in insects. In this study, we focused on the 3-hydroxy acyl-CoA dehydratase 2 (Hacd2), an important enzyme in the synthesis pathway of VLCFAs, in a cosmopolitan pest, the diamondback moth, Plutella xylostella. Hacd2 was cloned from P. xylostella and the relative expression pattern was identified. Epidermal permeability increased with the decreased VLCFAs in the Hacd2-deficient P. xylostella strain, which was constructed by using the CRISPR/Cas9 system. Survival and fecundity of the Hacd2-deficient strain was significantly lower than that of the wildtype strain when subject to desiccating environmental stress. Hacd2 mediates thermal adaptability in P. xylostella by changing epidermal permeability so is likely to be key to its remaining a major pest species under predicted climate change conditions.

Seasonal thermal comfort and adaptive behaviours for the occupants of residential buildings: Shaoxing as a case study

This study aimed to investigate the thermal comfort requirements in residential buildings and establish an adaptive thermal comfort model. A two-year field study was conducted on residential buildings in Shaoxing, Zhejiang Province, China. A total of 172 apartments (187 people) were surveyed, and 2400 valid questionnaires on thermal sensation from residents were obtained in two years. The results showed that the thermal comfort requirements of residents change with the seasons: Different seasons lead to changes in the range of thermal comfort zones and the residents’ thermal adaptation models, which are related to the climatic characteristics of each season. Compared with the traditional year-round adaptive thermal comfort model, seasonal models could reflect the status of the residents’ thermal adaptation caused by the characteristics of dynamic climate change more comprehensively and accurately. In addition, this study determined the residents’ psychological and behavioural adaptability to the seasons and found the characteristics of thermal sensation and requirements for thermal comfort in different seasons. In conclusion, this study provides people in the study area with basic knowledge of their thermal comfort requirements. The results could be used as a basis to improve energy efficiency and meet comfort needs in the future.