In reinforced 3D printed concrete structures, the shear interaction between the rebar and the 3D printed filaments influences both the performance and safety of the concrete structures during service. In this study, the shear performance of the interlayer reinforced interface (IRI) of 3D printed concrete with recycled coarse aggregates (3DPRAC) was investigated, focusing on the pore structure characteristics of the interlayer interface. An enhancement method for the IRI was proposed by varying the number of anchored rebar nails (ARNs) and comparing the results with those of 3D printed concrete with natural coarse aggregates (3DPNAC) and 3D printed mortar (3DPM). The results showed that the ARNs significantly improved the IRI shear strength of 3DPRAC. Specifically, the IRI shear strength of 3DPRAC was 6.1% lower than that of 3DPNAC but 43.6% higher than that of 3DPM. By examining the structural characteristics of the rebar-3DPRAC bonding area, a relationship between pore defects and shear strength was established. This established relationship led to the proposal of a partition model for the interlayer bonding interface. A finite element model of 3D printed concrete incorporating real pore structure characteristics was developed to analyze the stress distribution and damage characteristics of the interface under shear stress. The fracture mode induced by local pores with the crack propagation mechanism was also analyzed. Finally, a unified formula for calculating the shear strength of the 3DPRAC IRI was derived based on the principles of virtual work and plastic limit theory. This study provides theoretical support for engineering applications of 3D-printed reinforced concrete structures.