To determine the formula for biomimetic warm-mix regeneration and fulfill the requirements of a “high waste asphalt mixture content, high quality, and high level” for its usage in reclaimed asphalt pavement (RAP), this paper first determined the suitable preparation process and formula for biomimetic warm-mix regeneration based on orthogonal experiments and a gray correlation analysis. Then, the optimum dosage of the warm-mix regenerant was determined by a uniaxial penetration test, low-temperature splitting test, and freeze–thaw penetration test. The rutting test was conducted to characterize the high-temperature performance of the asphalt mixture. The Immersion Marshall Test and the freeze–thaw splitting test were used to characterize the water stability of the recycled asphalt mixture. The low-temperature small beam test was employed to study the low-temperature performance of the recycled asphalt mixture. The asphalt’s short-term and long-term aging processes were simulated using the rotary thin-film oven test (RTFOT) and the pressure aging test (PAV). The action mechanism of biomimetic warm-mix regeneration was revealed by Fourier-transform infrared spectroscopy (FTIR). Finally, a comprehensive thermal performance test was conducted on the aged asphalt after biomimetic warm-mix regeneration. The results showed that the self-made biomimetic warm-mix regeneration agent exhibited an excellent regenerative effect on RAP and significantly reduced the mixing temperature of the styrene–butadiene–styrene (SBS)-modified asphalt mixture. In addition, the self-made biomimetic warm-mix regeneration agent effectively improved the high- and low-temperature performance of the recycled asphalt mixture, but had no noticeable effect on the water stability. The suggested dosage of the biomimetic warm-mix regeneration agent was 6%, and the mixing temperature was 130 °C. The microscopic chemical analysis revealed that biomimetic warm-mix regeneration restored the performance of aged asphalt by supplementing the light component. The change rules of the chemical functional groups and the comprehensive thermal properties of the recycled mixture showed a good correlation with the change rules of its high- and low-temperature performance.
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