TF Systems Research Articles

Synthesis, Structural, and Cytotoxic Properties of New Water-Soluble Copper(II) Complexes based on 2,9-Dimethyl-1,10-Phenanthroline and Their One Derivative Containing 1,3,5-Triaza-7-Phosphaadamantane-7-Oxide.

A series of water-soluble copper(II) complexes based on 2,9-dimethyl-1,10-phenanthroline (dmphen) and mixed-ligands, containing PTA=O (1,3,5-triaza-7-phosphaadamantane-7-oxide) have been synthesized and fully characterized. Two types of complexes have been obtained, monocationic [Cu(NO3)(O-PTA=O)(dmphen)][PF6] (1), [Cu(Cl)(dmphen)2][PF6] (2), and neutral [Cu(NO3)2(dmphen)] (3). The solid-state structures of all complexes have been determined by single-crystal X-ray diffraction. Magnetic studies for the complex 1–3 indicated a very weak antiferromagnetic interaction between copper(II) ions in crystal lattice. Complexes were successfully evaluated for their cytotoxic activities on the normal human dermal fibroblast (NHDF) cell line and the antitumor activity using the human lung carcinoma (A549), epithelioid cervix carcinoma (HeLa), colon (LoVo), and breast adenocarcinoma (MCF-7) cell lines. Complexes 1 and 3 revealed lower toxicity to NHDF than A549 and HeLa cells, meanwhile compound 2 appeared to be more toxic to NHDF cell line in comparison to all cancer lines. Additionally, interactions between the complexes and human apo-transferrin (apo-Tf) using fluorescence and circular dichroism (CD) spectroscopy were also investigated. All compounds interacted with apo-transferrin, causing same changes of the protein conformation. Electrostatic interactions dominate in the 1/2 – apo- Tf systems and hydrophobic and ionic interactions in the case of 3.

Busbar monitoring system of the ITER DC busbars

In ITER, DC busbars will be used to connect the AC/DC converters to the superconducting coils of the magnet system; the total length will exceed 5 km. The busbars used in the TF, PF/CS and CC coil power supply systems are rated for continuous current up to 68 kA, 55 kA and 10 kA respectively. The busbars will be cooled by deionized water through inlet and outlet cooling water collectors. The water flow rate and temperature will be measured by sensors installed in outlet collectors because reduction or loss of water flow might cause excessive heating of the busbar conductors and, in extreme case, damage their insulation. To prevent this, the Busbar Monitoring System was developed at the Efremov Institute for continuous monitoring of thermal conditions of the busbars. This paper presents the architecture of the Busbar Monitoring System, the detailed description of its components and results of their testing. The successful results of the type tests confirmed suitability of the design and compliance with ITER requirements which together with completion of the final design review makes it possible to start manufacturing of Busbar Monitoring System components for delivery to the ITER Site.

Control and operation strategy design of ITER coil power supply system

The large-scale ITER coil power supply system will generate huge current for superconducting magnets by plenty of AC/DC converters, which are based on thyristor technology on various circuits. A comprehensive consideration on design, manufacturing, testing and commissioning is the foundation to guarantee the safety operation of the CPSS system. This paper presents the design of converters control and operation strategies according to different typical circuit topologies. As well as illustrate the corresponding operation systems with associated functions to achieve those operation strategies. 2-Quadrant, 4-Quadrant and sequential control operation strategies are applied in corresponding PF (Poloidal Field), CS (Central Solenoid), CC (Correction Coil) and VS1 (Vertical Stability) converters with associated current closed-loop or voltage open-loop control during plasma operation. Three individual operation systems are comprised of TF, PF/CS and CC conventional systems with two architecture hierarchies to perform the control strategies and fulfill the real-time communication with central system. Typical simulation and test results are presented to verify the control strategies in accordance with the manufacturing and factory acceptance test. The converters will be installed in the ITER site soon and all the control strategies will be tested on the following commissioning phase.

Durability of Two R-Phase Rotary Systems used in the Mechanical Instrumentation of Dilacerated Root Canals

This study was aimed to analyze, through an ex vivo test, the durability of two rotary systems used in curved root canals of extracted molars. At first, 150 upper and lower molars were selected, presenting curvature at least in one of the root canals. They underwent access cavity and radiographed with a # 15 file into the canal to determine their maximum curvatures, classified as schneider. The teeth with one or more curved canals were included in this study. Finally, 120 curved root canals were statistically divided into six sets of 20 curved canals. Two rotatory systems, K3 XF (RCS sequence) and twisted file (Large Kit), both treatment R-Phase, were analyzed. Three kits were used for each system, each one instrumented one of the six sets of 20 curved canals. File deformations were noted, and the files were used until a fracture occurs or until completion of 20 canals preparation. Fractured files before the end of the instrumentation of the 20 canals were replaced, continuing the full instrumentation set. Statistical analysis was performed by analysis of variance (ANOVA) (p >0.05), and a qualitative assessment was also made. It was found that there was no statistical difference between the K3 XF and TF systems regarding fracture. There were no statistical differences between the K3 XF systems in the RCS sequence and the twisted file kit Large system, as to the number of instrumented conduits to the deformation or fracture. The findings of this study reinforce that, it is necessary to choose files that support curved roots preventing accidents inside the root canal. Therefore, it is important for professionals to have knowledge and command of these protocols to obtain more satisfactory results.

Ignition of Some Powder Mixtures of Metals with Teflon

The dependence of ignition and the formation of the product structure in powder mixtures of W–Ti, Ti–B, Ti–Cu, and Ni–Al metals with Teflon (Tf) on the blending method and heating rate is studied. The increase in the heating rate is shown to affect slightly the ignition temperature of the Ti–B–Tf and Ti–Cu–Tf systems. However, it significantly changes the structure of the reaction products, leading to the formation of a large amount of melt. Compositions forming a large volume of gaseous products in the process of ignition and combustion fly out completely (W–Ti–Tf) or form a highly porous structure. Estimates of the activation energy of the process are obtained for the weakly energetic Ti–Cu–Tf system.

Shaping ability of several nickel–titanium systems in double-curved simulated canals

AimThe aim of the study was to evaluate the shaping ability of four nickel–titanium systems (iRaCe, ProTaper Next, Wave One, and TF) in simulated double-curved (S-shaped) root canals. Materials and methodsThis study was conducted using simulated S-shaped root canals (n=40). The specimens were divided into four experimental groups (n=10 each). Each group was prepared using one of the following NiTi systems: iRaCe, ProTaper Next, Wave One, and TF. The canals were injected with black ink before instrumentation, and a series of photographs of each canal was saved to a computer using a set protocol. The canals were reinjected using red ink postoperatively to define their outlines, and images were taken in the same standardized manner. Photoshop software was used to superimpose pre- and post-instrumentation images in two different layers. Canal transportation was evaluated at five positions from the apical end (D1, D2, D3, D5, and D7) by measuring the amount of removed resin using ImageJ Software. The data were analyzed using SPSS ver. 20.0. Statistical significance was set at P<0.05. ResultsThere were no significant differences (P>0.05) in shaping time (in seconds) among the NiTi systems, and there were no significant differences (P>0.05) in canal transportation values among the systems at D1, D2, D3, D5, and D7 among the NiTi systems. ConclusionThe iRaCe, ProTaper Next, Wave One, and TF systems preserved the original shape of the double-curved (S-shaped) canal with minimum root canal transportation. These systems produced satisfactory root canal instrumentation in S-shaped canals.

Comparison of root canal apical transportation associated with Wave One, ProTaper Next, TF, and OneShape nickel–titanium instruments in curved canals of extracted teeth: A radiographic evaluation

Abstract Aim: The aim of the present study was to compare apical transportation in curved root canals prepared using ProTaper Next, Wave One, TF, and OneShape nickel–titanium [NiTi] systems. Materials and methods: This study was conducted on mesiobuccal canals of extracted first and second mandibular molars with 30–60 degree curvatures [ n = 40]. Specimens were divided into four experimental groups [ n = 10 each]. In each group, the mesiobuccal canals were prepared using NiTi systems, including Wave One, ProTaper Next, TF, and OneShape. The apical transportation with each NiTi system was measured using a double digital radiograph technique. The data were analyzed by ANOVA using SPSS ver. 20.0, and significance was set at P Results: There were significant differences in apical transportation [ P P > 0.05] in apical transportation between TF and OneShape or between ProTaper Next and Wave One. Conclusions: TF and OneShape NiTi systems preserved the original shape of the canal in the apical area better than ProTaper Next and Wave One NiTi systems did.

Vertical Load Induced with Twisted File Adaptive System during Canal Shaping

IntroductionTo evaluate the vertical load induced with the Twisted File Adaptive (TFA; SybronEndo, Orange, CA) system during canal shaping of extracted teeth by comparing it with the Twisted File (TF, SybronEndo), ProTaper Next (PTN; Dentsply Maillefer, Ballaigues, Switzerland), and ProTaper Universal (PTU, Dentsply Maillefer) systems. MethodsFifty-two root canals were shaped using the TFA, TF, PTN, or PTU systems (n = 13 for each system). They were shaped gently according to the manufacturers' instructions. During canal shaping, vertical loads were recorded and shown in 2 directions, apically and coronally directed loads. The vertical peak loads of 3 instrumentation stages were used for comparison. The effects of rotary systems on the mean positive and negative peak loads were analyzed statistically using the Kruskal-Wallis and Mann-Whitney tests at a confidence level of 95%. ResultsThe overall pattern of the instantaneous loads appeared to increase with the use of successive instruments within the system. During canal shaping in all groups, the apically and coronally directed peak loads ranged from 0.84–7.55 N and 2.16–2.79 N, respectively. There were significant differences in both peak loads among the tested systems at each instrumentation stage. TFA had the lowest apically directed peak loads. In terms of coronally directed peak loads, the TFA and TF had a significantly lower amount of loads developed with their instruments than PTN and PTU. ConclusionsThe choice of instrument system had an influence on the loads developed during canal shaping. TFA instruments were associated favorably with the lowest values of peak loads followed by TF, PTN, and PTU.

ITER Side Correction Coil Quench model and analysis

Previous thermohydraulic studies performed for the ITER TF, CS and PF magnet systems have brought some important information on the detection and consequences of a quench as a function of the initial conditions (deposited energy, heated length). Even if the temperature margin of the Correction Coils is high, their behavior during a quench should also be studied since a quench is likely to be triggered by potential anomalies in joints, ground fault on the instrumentation wires, etc. A model has been developed with the SuperMagnet Code (Bagnasco et al., 2010) for a Side Correction Coil (SCC2) with four pancakes cooled in parallel, each of them represented by a Thea module (with the proper Cable In Conduit Conductor characteristics). All the other coils of the PF cooling loop are hydraulically connected in parallel (top/bottom correction coils and six Poloidal Field Coils) are modeled by Flower modules with equivalent hydraulics properties. The model and the analysis results are presented for five quench initiation cases with/without fast discharge: two quenches initiated by a heat input to the innermost turn of one pancake (case 1 and case 2) and two other quenches initiated at the innermost turns of four pancakes (case 3 and case 4). In the 5th case, the quench is initiated at the middle turn of one pancake. The impact on the cooling circuit, e.g. the exceedance of the opening pressure of the quench relief valves, is detailed in case of an undetected quench (i.e. no discharge of the magnet). Particular attention is also paid to a possible secondary quench detection system based on measured thermohydraulic signals (pressure, temperature and/or helium mass flow rate). The maximum cable temperature achieved in case of a fast current discharge (primary detection by voltage) is compared to the design hot spot criterion of 150K, which includes the contribution of helium and jacket.

Cool Down Experiences with the SST-1 Helium Cryogenics System before and after Current Feeders System Modification

The SST-1 machine comprises a superconducting magnet system (SCMS), which includes TF and PF magnets. In order to charge the SCMS, we need superconducting current feeders consisting of SC feeders and vapor cooled current leads (VCCLs). We have installed all 10 (+/-) pairs of VCCLs for the TF and PF systems. While conducting initial engineering validation of the SST-1 machine, our prime objective was to produce circular plasma using only the TF system. During the SST-1 campaign I to VI, we have to stop the PF magnets cooling in order to get the cryo- stable conditions for current charging of the TF magnets system. In that case, the cooling of the PF current leads is not essential. It has been also observed that after aborting the PF system cooling, there was a limited experimental window of TF operation. Therefore, in the recent SST-1 campaign-VII, we removed the PF current leads (9 pairs) and kept only single (+/-) pair of the 10,000 A rated VCCLs to realize the charging of the TF system for the extended window of operation. We have observed a better cryogenic stability in the TF magnets after modifications in the CFS. In this paper, we report the comparison of the cool down performance for the SST-1 machine operation before and after modifications of the current feeders system.

Characterization of the binary mixed monolayers of α-tocopherol with phospholipids at the air-water interface

The mixed Langmuir monolayers composed of model constituents of biological membranes, 1,2-dipalmitoyl-sn-glycero-3-phosphocholine (DPPC), 2-oleoyl-1-palmitoyl-sn-glycero-3-phosphocholine (POPC), and 1,2-dioleoyl-sn-glycero-3-phosphocholine (DOPC), were investigated to provide information on the intermolecular interactions between these membrane components and the physiologically active vitamin E–α-tocopherol (TF), as well as on the phase behavior of these mixed systems. Additionally, topography of these monolayers transferred onto the mica support was investigated by the inverted metallurgical microscope. Morphological characteristics were directly observed by Brewster angle microscopy (BAM). From the surface pressure–area isotherms and the analysis of physicochemical parameters (compressibility and mean molecular area at the maximum compressibility) it was found that depending on the acyl chains saturation degree, TF has different effect on the phospholipids. In the case of DPPC, the addition of TF to the phospholipid film causes destabilization of the ordered hydrocarbon chains, while in the POPC/DOPC–TF systems, the attractive interactions are responsible for the monolayer increased stability. Thus, the results of these studies confirm the hypothesis that α-tocopherol may play a role in the stabilization of biological membranes.

Thermostats and thermostat strategies for molecular dynamics simulations of nanofluidics

The thermostats in molecular dynamics (MD) simulations of highly confined channel flow may have significant influences on the fidelity of transport phenomena. In this study, we exploit non-equilibrium MD simulations to generate Couette flows with different combinations of thermostat algorithms and strategies. We provide a comprehensive analysis on the effectiveness of three thermostat algorithms Nosé-Hoover chain (NHC), Langevin (LGV) and dissipative particle dynamics (DPD) when applied in three thermostat strategies, thermostating either walls (TW) or fluid (TF), and thermostating both the wall and fluid (TWTF). Our results of thermal and mechanical properties show that the TW strategy more closely resembles experimental conditions. The TF and TWTF systems also produce considerably similar behaviors in weakly sheared systems, but deviate the dynamics in strongly sheared systems due to the isothermal condition. The LGV and DPD thermostats used in the TF and TWTF systems provide vital ways to yield correct dynamics in coarse-grained systems by tuning the fluid transport coefficients. Using conventional NHC thermostat to thermostat fluid only produces correct thermal behaviors in weakly sheared systems, and breaks down due to significant thermal inhomogeneity in strongly sheared systems.

Shaping ability of thermomechanically treated files in simulated S-shaped root canals

Aim: the aim of this study was to investigate the shaping ability of thermomechanically treated files manufactured by twisting(Twisted files)and compare it to conventional rotary system (K3, Sybron Endo, Orange, CA) in S-shaped canals, including formation of ledges, zipping, elbow, outer widening, danger zone, perforation and file deformation. Materials & Methods: Forty S-Shaped canals in resin blocks were randomly divided into 2 groups of 20 each. Pre-instrumentation images of the canals were taken via a digital camera and superimposed on images taken after preparation with TF and K3 systems to apical size of 25/06 and 30/06. Canal aberrations were measured from the superimposed image at five levels using AutoCAD system. Fisher exact test and Mann Whitney test were used for analysis of the data. Results: the incidence of zipping, elbow and apical transportation was significantly lower in the TF group (P = 0.04). Generally the incidence of aberration increased when the apical size increased to 30/0.06 regardless of the file system. Significant file deformation was evident in the TF after single use (P ? 0.001). Conclusion: Under the conditions of this study, TF manufactured by new technique performed better than K3 systems when used up to size 25/06 in simulated S-shaped canals. Clinical significance: The flexibility of thermomechanically treated files is beneficial in canals with multiple curvatures; however, attention should be paid to the instrument taper and final apical size of the preparation.

A Comparison of Apical Transportation between FlexMaster and Twisted Files Rotary Instruments

IntroductionThe aim of this study was to evaluate apical transportation in root canals after the use of Twisted Files (TF; SybronEndo, Orange, CA) and FlexMaster (VDW, Munich, Germany) #40/04 rotary files. MethodsA double-digital radiographic technique was used to compare apical transportation between the TF and FlexMaster systems. Each rotary system was used to instrument mesial canals from 80 extracted mandibular molars. The central axes of the file imaged before instrumentation (#15 K-file) and the master apical rotary file (#40/04) were superimposed digitally. AutoCAD 2008 (Autodesk Inc, San Rafael, CA) was used to measure apical transportation at 0.5 mm from the working length (WL). The data were analyzed using the Student's t test, and significance was set at P < .05. ResultsThe mean amount of apical transportation at 0.5 mm from the WL was 0.17 ± 0.09 mm for the FlexMaster group and 0.19 ± 0.12 mm for the TF group. No statistically significant differences in apical transportation were found between the 2 groups. ConclusionsUnder the conditions of the study, no statistically significant differences in apical transportation were observed between FlexMaster and TF rotary files.

Genetic position of Chuvashes in the system of Finno-Ugric and Turkic-speaking peoples

Serological and biochemical polymorphisms in marker genes of the AB0, MN, RH, FY, HP, TF, ACP1, PGM1, ESD, and GLO1 systems were studied in the combined sample of 369 individuals of Chuvash ethnicity from Morgaushskii, Mariinsko-Posadskii and Yadrinskii districts of the Chuvash Republic. We have compared our results with the data obtained in previous studies by other authors. A linear relationship has been established between genetic and geographical distances by examining 11 ethnoterritorial groups in Northeastern Europe and Western Siberia.

Genetic polymorphisms in autochthonous Basques from Northern Navarre

This survey reports primary results of classical allele frequencies on ten protein loci in a Basque population sample from northern Navarre, the less known from an anthropological and genetic point of view than the populations of the other Basque territories of Spain. Since ancient times this has been a zone of Basque population settlement, and the Basque language (Euskera) still remains deeply rooted among its autochthonous population. A total of 122 blood samples from unrelated northern Navarrese with autochthonous ascendants to the third generation were typed for GC, HP, PI, TF, ACP1, AK1, CA2, ESD, PGD and PGM1 genetic systems. Basque surnames and birthplaces were the criteria used to define family origins. Genetic structure was analyzed on different population hierarchical levels. Northern Navarre seems to be the most genetically deviated area in comparison with other Basque groups. The highest level of differentiation is observed between Navarrese and Alava Basques whereas Guipúzcoa province, the territory adjacent to northern Navarre, presents the lowest genetic distance from the study area. Northern Navarrese show some distinguishing genetic characteristics in relation to other Basque relative samples, which include high frequencies for PI*M1 and TF*C1 and low levels of PGD*C and PGM1*2 alleles. When the genetic data reported here are analyzed jointly with GM allotypes frequencies, the results significantly reinforce the relative position of Navarrese Basques as well as the topology of the Basque cluster on genetic maps. The analysis of relationships among the genetic structures of Basque population samples leads us to ask ourselves which of them fits in best with the ancient Basque population. Classical geographers placed the tribe of the Vascones in the geographical region currently known as Navarre, so extant Navarrese Basques might be considered firm candidates to denote the anthropological and genomic distinctiveness of the ancient Basques.

Potential of Transferrin and Transferrin Conjugates of Liposomes in Drug Delivery and Targeting

Targeted drug delivery has gained recognition in modern therapeutics and attempts are being made to explore the potential of cell biology-related bioevents in the development of specific and target-oriented systems. In connection to modern therapeutic systems, most of the emphasis has been laid upon the bioconjugated drug delivery systems. Bioconjugates involve the linking of two or more molecules to form a novel complex having the combined properties of its individual components. The nature of the linking agent between the pharmacologic agent and the delivery-augmenting moiety dictates the degree of successful delivery and its outcome. The component for the bioconjugated drug delivery includes receptors and ligands, where the receptors act as molecular targets or portals whereas ligands with receptors provide selective and specific trafficking towards the targeting site. Recently, a number of bioconjugated systems have been discovered for the site-specific presentation and delivery of various bioactive substances using biorelevant ligands, including antibodies, glycoprotein, viral proteins, and molecules of endogenous origin. In this review, the potential of transferrin (Tf) and Tf conjugates of liposomes in site-specific drug delivery systems are discussed. Tf is an abundant component of serum with the capacity to bind and transport iron, while Tf receptor (TfR), a dimeric transmembrane glycoprotein, is present on the surface of the most proliferating, higher eukaryotic cells. Tf expression is also found in nonproliferating tissues, such as hepatocytes, tissue macrophages, pituitary cells, pancreatic islet cells, and the endothelium of brain capillaries. Tumor cells frequently carry elevated numbers of TfRs compared with corresponding normal cells, and reduced serum levels of Tf are often observed in patients with tumors. In the past, various strategies have been developed, which include coupling of the liposomal surface with Tf by using various linking agents. Low-molecular weight drugs and proteins as well as liposomes can be linked with Tf. The Tf-coupled vesicular system is physicochemically stable in the bioenvironment and is site-specific. The aim of coupling liposomes with Tf is to improve the physical and biochemical stability of liposomes and make them appropriate for targeting specific organs and cells. Tf may be widely applied either as a carrier or targeting ligand in the active targeting of anticancer agents, proteins, and genes to primarily proliferating malignant cells that overexpress TfRs. Tf has been used as a molecular conjugate to deliver DNA to erythroleukemic, lung, and liver cell lines. Tf can also be modified with the positive charge N-acylurea groups to make them suitable for electrostatic binding of DNA, in order to achieve a well defined DNA-binding ligand for receptor-mediated gene transfer. Association of Tf with lipoplexes, in particular the negatively charged ternary complexes, significantly overcomes the inhibitory effect of serum and facilitates efficient transfection in many cell lines, including HeLa, K-562 cells, and lung carcinoma cells Calu-3 and H-292 cells. Tf-lipoplex has demonstrated high efficiency in tumor-targeted gene delivery and long-term therapeutic accuracy in systemic p53 gene therapy for both human head and neck cancer and prostate cancer. Tf and Tf-coupled liposomal drug delivery systems may prove particularly valuable to enable the use of a drug that seems to be ineffective or toxic if delivered systematically. The delivery of drugs to the brain has been particularly challenging because of the presence of the blood-brain barrier, which restricts the passage of most therapeutic agents into the brain. Therefore, active targeting of the brain is crucial for effective treatment of brain diseases. The anti-TfR antibody, such as OX26, when coupled with therapeutic agents, has shown potential in drug and gene delivery to the brain.

Fusion power core engineering for the ARIES-ST power plant

ARIES-ST is a 1000 MWe fusion power plant based on a low aspect ratio ‘spherical torus’ (ST) plasma. The ARIES-ST power core was designed to accommodate the unique features of an ST power plant, to meet the top-level requirements of an attractive fusion energy source, and to minimize extrapolation from the fusion technology database under development throughout the world. The result is an advanced helium-cooled ferritic steel blanket with flowing PbLi breeder and tungsten plasma-interactive components. Design improvements, such as the use of SiC inserts in the blanket to extend the outlet coolant temperature range were explored and the results are reported here. In the final design point, the power and particle loads found in ARIES-ST are relatively similar to other advanced tokamak power plants (e.g. ARIES-RS [Fusion Eng. Des. 38 (1997) 3; Fusion Eng. Des. 38 (1997) 87]) such that exotic technologies were not required in order to satisfy all of the design criteria. Najmabadi and the ARIES Team [Fusion Eng. Des. (this issue)] provide an overview of ARIES-ST design. In this article, the details of the power core design are presented together with analysis of the thermal–hydraulic, thermomechanical and materials behavior of in-vessel components. Detailed engineering analysis of ARIES-ST TF and PF systems, nuclear analysis, and safety are given in the companion papers [4–7].

Blood protein polymorphisms in the donkey (Equus asinus)

Transferrin, albumin, 6-phosphogluconate dehydrogenase and vitamin D-binding protein polymorphisms were detected in 242 feral and domesticated Australian donkeys by polyacrylamide gel electrophoresis, starch gel electrophoresis, autoradiography, immunoblotting with specific antisera and activity staining. All four TF and two ALB variants were donkey specific while only one of the PGD variants was donkey specific. The two GC variants were electrophoretically identical to the Equus caballus F and S proteins. Available evidence suggested that the TF, ALB, PGD and GC systems are controlled by co-dominant alleles with frequencies of the most common alleles of each system being 0.831, 0.946, 0.957 and 0.861 respectively. Glucose phosphate isomerase and plasminogen were monomorphic in the Australian population of donkeys.

PGM1 and TF subtypes and C3 polymorphisms in Continental Italy and Sardinia. Data on the world distribution of these genetic markers

PGM1, TF and C3 polymorphisms have been examined in two Italian samples, collected in continental Italy and in Sardinia (Cagliari). The PGM1 and TF subtypes were determined by isoelectric focusing while the C3 was studied by conventional methods. A significant difference in the gene frequencies of PGM1 and TF systems between our two samples has been observed. In addition, data on the world distribution of PGM1, TF and C3 polymorphisms have been presented.