The use of organic fertilizers to replace chemically synthesized fertilizers has assumed an important role in managing plant nutrition and soil fertility. The various organic matrices currently available as organic byproducts and digestates are relatively abundant and have shown promising effects in terms of plant-available nutrients. However, like mineral fertilizers, organic fertilizers must be carefully managed to avoid negative effects on the environment, especially when they are repeatedly applied over time. The aim of the present study was to assess the effect of the single (DIG) and repeated application (DIGP) of solid anaerobic digestates compared to an unamended control (CTR) on the denitrifying enzymatic activity (DEA), which is responsible for nitrous oxide emissions into the atmosphere, and some related soil properties, such as total soluble nitrogen (TSN), nitrate (NO3−-N), extractable carbon (Cextr), microbial biomass carbon (MBC), and basal respiration (Rbas), for a period of ~3 months after application. The application of solid anaerobic digestates progressively boosts N and C concentrations in the soil, with the degree of enhancement directly correlated with the frequency of application over the sampling period. Depending on the textural properties of soils, there was a notable rise in denitrification enzyme activity (DEA), particularly during the DIGP treatment, suggesting that clay soils are highly susceptible to denitrification under suitable conditions. The results of this study recommend the careful management of soils subjected to repeated digestate amendment to prevent the occurrence of conditions conducive to denitrification and the promotion of N2O emissions.
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