Tetrakis Hydroxymethyl Phosphonium Sulfate Research Articles

Elevated water transport of polyamide nanofiltration membranes via aqueous organophosphorus mediated interfacial polymerization

As interfacial polymerization (IP) is a reaction-diffusion process, rational regulation of diamine diffusion rate is effective to heighten the nanofiltration (NF) performance of polyamide (PA) membranes. Herein, we propose an aqueous organophosphorus-regulated IP strategy that promotes the formation of thinner and moderately crosslinked films toward improved water transport. Two similar compounds tetrakis (hydroxymethyl) phosphonium chloride (THPC) and tetrakis (hydroxymethyl) phosphonium sulfate (THPS) were utilized as functional additives to physically interact with piperazine via hydrogen bonding and thus retard piperazine diffusion rate during an IP process. The resultant polyamide membranes have been analyzed in terms of surface morphologies, chemical structures, hydrophilicity, and separation performance in detail. Surface temperature monitoring results revealed that heat release from regulated IP was less than that from unmodified IP, which was ascribed to retard monomer diffusion toward organic boundary in the presence of THPC or THPS. Molecular dynamics simulation further confirmed that the addition of THPC and THPS could efficiently decline the monomer diffusion rate via hydrogen bonds and increased solution viscosity. The modified PA membranes exhibited a reduced film thickness, moderate crosslinking degree, as well as increased average pore size, as revealed by a series of characterizations. Furthermore, the incorporation of THPC or THPS resulted the PA membranes with higher surface hydrophilicity and stronger negative charges. These improved physicochemical features synergically conferred a two-fold water permeance while maintaining a high divalent salt rejection (THPC-0.01: 18.3 L m-2 h−1 bar−1, 99.0 %, THPS-0.015: 20.2 L m−2 h−1 bar−1, 98 %). This aqueous organophosphorus regulated IP offers a simple and feasible approach to the preparation of low-transport-resistance PA membranes for enhanced NF performance.

PH responsive THPS crosslinked injectable hydrogel with Cu-metformin sustained-release for accelerating wound healing

The utilitarian capacity of hydrogels in fostering a conducive milieu for cellular viability renders them a compelling alternative to conventional wound dressings. Nonetheless, the uncontrolled drug release exhibited by numerous drug-loaded hydrogel dressings often undermines their capacity for sustaining therapeutic efficacy over prolonged periods, while certain hydrogel formulations prove inadequate for addressing deep-seated wounds with low pH caused by chronic bacterial infections. Within the scope of this investigation, a novel pH-responsive injectable sustained-release hydrogel is developed through the crosslinking of tetrakis (hydroxymethyl) phosphonium sulfate (THPS) with carboxymethyl chitosan (CMCS) and Ɛ-poly-L-lysine (EPL). This innovative hydrogel manifests rapid gelation within a span of 30 s sans the requirement of a catalyst, alongside demonstrating commendable antibacterial attributes facilitated by the incorporation of a Cu-metformin complex (CuMet). Furthermore, the lyophilized hydrogel exhibits exceptional water absorbency and hemostatic qualities, effectively addressing exudative concerns and mitigating blood loss. In vitro assessments ascertain the higher drug release under acidic conditions and sustained drug release potential of CMCS/EPL/CuMet for up to 120 h, concomitantly expediting wound healing processes through the attenuation of bacterial colonization and inflammatory responses. In summation, this investigation furnishes novel insights into the viability of the developed formulation as a sustained-release injectable wound dressing.

Acceleration mechanism of riboflavin on Fe0-to-microbe electron transfer in corrosion of EH36 steel by Pseudomonas aeruginosa

Riboflavin (RF), as a common electron mediator that can accelerate extracellular electron transfer (EET), is usually used as a probe to confirm EET-microbiologically influenced corrosion (MIC). However, the acceleration mechanism of RF on EET-MIC is still unclear, especially the effect on gene expression in bacteria. In this study, a 13-mer antimicrobial peptide E6 and tetrakis hydroxymethyl phosphonium sulfate (THPS) were used as new tools to investigate the acceleration mechanism of RF on Fe0-to-microbe EET in corrosion of EH36 steel caused by Pseudomonas aeruginosa. 60 min after 20 ppm (v/v) THPS and 20 ppm THPS & 100 nM E6 were injected into P. aeruginosa 1 and P. aeruginosa 2 (two glass bottles containing P. aeruginosa with different treatments) at the 3-d incubation, respectively, P. aeruginosa 1 and P. aeruginosa 2 had a similar planktonic cell count, whereas the sessile cell count in P. aeruginosa 1 was 1.3 log higher than that in P. aeruginosa 2. After the 3-d pre-growth and subsequent 7-d incubation, the addition of 20 ppm (w/w) RF increased the weight loss and maximum pit depth of EH36 steel in P. aeruginosa 1 by 0.7 mg cm−2 and 4.1 μm, respectively, while only increasing those in P. aeruginosa 2 by 0.4 mg cm−2 and 1.7 μm, respectively. This suggests that RF can be utilized by P. aeruginosa biofilms since the corrosion rate should be elevated by the same value if it only acts on the planktonic cells. Furthermore, the EET capacity of P. aeruginosa biofilm was enhanced by RF because the protein expression of cytochrome c (Cyt c) gene in sessile cells was significantly increased in the presence of RF, which accelerated EET-MIC by P. aeruginosa against EH36 steel.

A multifunctional protein-based hydrogel with Au nanozyme-mediated self generation of H2S for diabetic wound healing

Diabetics usually suffer from chronic impaired wound healing due to facile infection, excessive inflammation, diabetic neuropathy, and peripheral vascular disease. Hence, the development of effective diabetic wound therapy remains a critical clinical challenge. Hydrogen sulfide (H2S) regulates inflammation, oxidative stress, and angiogenesis, suggesting a potential role in promoting diabetic wound healing. Herein, we propose a first example of fabricating an antibiotic-free antibacterial protein hydrogel with self-generation of H2S gas (H2S-Hydrogel) for diabetic wound healing by simply mixing bovine serum albumin‑gold nanoclusters (BSA-AuNCs) with Bis[tetrakis(hydroxymethyl)phosphonium] sulfate (THPS) at room temperature within a few minutes. In this process, the amino group in BAS and the aldehyde group in THPS are crossed together by Mannich reaction. At the same time, tris(hydroxymethyl) phosphorus (trivalent phosphorus) from THPS hydrolysis could reduce disulfide bonds in BSA to sulfhydryl groups, and then the sulfhydryl group generates H2S gas under the catalysis of BSA-AuNCs. THPS in H2S-Hydrogel can destroy bacterial biofilms, while H2S can inhibit oxidative stress, promote proliferation and migration of epidermal/endothelial cells, increase angiogenesis, and thus significantly increase wound closure. It would open a new perspective on the development of effective diabetic wound dressing.

Eco-friendly preparation of advanced epoxy composites and their pyrolysis and flame retardant mechanisms

Traditional additive flame retardants pose issues including high dosage, easy precipitation, and poor compatibility with the matrix. Designing efficient organic-inorganic synergistic flame retardants can enhance the flame retardancy and mechanical properties of epoxy (EP) composites while reducing their smoke toxicity. In our study, we initially employed Tetrakis(hydroxymethyl)phosphonium sulfate (THPS) for intercalation modification of montmorillonite (MMT). Subsequently, we achieved a green and efficient exfoliation of MMT by reacting urea with hydroxyl groups, resulting in the simultaneous preparation of efficient organic/inorganic synergistic flame retardants (THPSU-MMT). EP composites containing 5wt% THPSU-MMT can attain UL-94 V0 classification. Furthermore, the peak heat release rate (PHRR), total heat release (THR), and peak release rates of CO can be reduced by up to 78.42 %, 62.26 %, and 72.50 %, respectively. Simultaneously, we systematically investigated the synergistic flame retardant mechanism of the EP composite. The findings reveal that THPSU-MMT exhibits a dual flame retardant effect in both the gaseous and condensed phases. Moreover, the combination of MMT and THPSU substantially enhances the presence of phosphorus-containing pyrolysis products in the condensed phase. The fire performance index (FPI) of the flame retardant EP increased from 0.05 m2/s/W in pure EP to 0.36 m2/s/W, attributed to the enhanced catalytic carbonization and assisted gas-phase free radical capture. Additionally, the flame spread index (FGI) decreased from 11.55 kW/m/s in pure EP to 1.18 kW/m/s, signifying a substantial enhancement in fire safety. Furthermore, the THPSU-MMT synergistic flame retardants improved the tensile strength of EP composites. This study offers an environmentally friendly and efficient approach for the effective exfoliation of MMT, while also presenting novel concepts for the design of efficient organic-inorganic synergistic flame retardants.

Effect of tetrakis(hydroxymethyl)phosphonium sulfate (THPS) on the microbial community and corrosion of carbon steel in a simulated crude-oil storage tank environment

The biocorrosion process has been a problem for the oil and gas industry, involving deterioration of facilities, loss of oil quality, metal loss, and oil leaks. In oil storage tanks, the presence of a metallic surface along with crude oil and water poses a biocorrosion risk. Biocides are used to control the biocorrosion of different metallic infrastructures, tetrakis(hydroxymethyl)phosphonium sulfate (THPS) is commonly used due to its low environmental impact and its effectiveness in preventing sulfate-reducing bacteria (SRB) growth. The treatment effect with one THPS application on microbial community and corrosion rates was evaluated in a batch fractionated assay containing water from a crude oil storage tank, carbon steel coupons and crude oil. The effects of the treatment were monitored for 60 days. The number of planktonic bacteria and archaea estimated by qPCR decreased at the beginning of the treatment with THPS, however, 60 days after the application their number was higher, as were the sulfate-reducing microorganisms (SRM) and methanogens (MA). The diversity analysis of the 16S rRNA gene showed that the relative abundance of the genus Desulfovibrio and Methanocalculus was higher on day 8 in the presence of THPS, but by day 60 there were no differences. The biocide did not inhibit bacterial and archaeal biofilm attached to the SAE 1010 carbon steel coupons and produced a decrease in corrosion rates by blocking the surface. The proximity of the corrosion potential to the pitting potential indicated a high susceptibility to localized corrosion. The results suggested that the changes in the microbial community by THPS treatment were related to the corrosion rates of carbon steel, rather than the number of microorganisms attached to the coupon.

Preliminary corrosion prevention of microbiologically induced corrosion in high producer main oil pipelines to hinder reoccurrence leak

Microbiologically induced corrosion (MIC) is a prominent issue in high-producer main oil lines (MOL) despite the stringent mechanical and chemical treatment due to Sulfate-reducing bacteria (SRB). Monthly corrosion monitoring shows the number of bacteria on the pig body and in seawater remains significant. The investigation corresponds with the dormancy and duplication of SRB retains in siloes due to uncontrolled parameters such as injection schedule and the ideal biocide dosage. While investigating and predicting the resistant bacteria that may threaten the pipeline is an attractive discussion, modelling the corrosion mitigation using a corrosion test is equally essential to predict the effectiveness of biocides. Biocide performance was evaluated in a few test solutions from two locations in one of the well-established oil company producers in northwest Java. The corresponding time-kill test using Tetrakis (hydroxymethyl) Phosphonium Sulfate (THPS) adheres to NACE TM- 0194 (2014) standard to obtain the optimum biocide dosing of the bacteria solution. Biofilm and micro-pitting were identified by evaluating the blank and 1000 μg/L 60% THPS solutions using a Scanning Electronic Microscope (SEM). Based on the time kill test, the inactive bacteria in dormancy after injection 500 μg/L and 1000 μg/L THPS 60%. The wheel corrosion test shows ineffective injection increases chemical acidity and corrosion rate. The SEM result confirms the time-kill test and indicates the possible sessile bacteria and biotic pitting. This work exemplifies the comprehensive engagement between the field operation and experimental work to quicken the MIC analysis and recommend a viable strategy for the parameter field variation.

Mitigation of Desulfovibrio ferrophilus IS5 degradation of X80 carbon steel mechanical properties using a green biocide.

Most microbiologically influenced corrosion (MIC) studies focus on the threat of pinhole leaks caused by MIC pitting. However, microbes can also lead to structural failures. Tetrakis hydroxymethyl phosphonium sulfate (THPS) biocide mitigated the microbial degradation of mechanical properties of X80 steel pipeline by Desulfovibrio ferrophilus (IS5 strain), a very corrosive sulfate reducing bacterium. It was found that 100 ppm (w/w) THPS added to the enriched artificial seawater (EASW) culture medium before incubation resulted in 2.8-log reduction in sessile cell count after a 7-d incubation at 28 °C under anaerobic conditions, leading to 94% uniform corrosion rate reduction (from 1.3to 0.07mm/a), and 84% pitting corrosion rate reduction (from 0.70to 0.11mm/a). The X80 dogbone coupon incubated with 100 ppm THPS for 7 d suffered 3% loss in ultimate tensile strain and 0% loss in ultimate tensile strength compared with the abiotic control in EASW. In comparison, the no-treatment X80 dogbone coupon suffered losses of 13% in ultimate tensile strain and 6% in ultimate tensile stress, demonstrating very good THPS efficacy.

Eco-friendly bifunctional antibacterial and anticorrosive broad-spectrum rosin thiourea iminazole quaternary ammonium salt against microbiologically influenced corrosion

Bifunctional antibacterial and anticorrosive broad-spectrum rosin thiourea iminazole quaternary ammonium salt (RTIQAS) was investigated to inhibit microbiologically influenced corrosion (MIC) caused by anaerobic gram-negative sulfate reducing bacterium (SRB) Desulfovibrio vulgaris against X80 carbon steel and aerobic gram-positive bacterium Bacillus licheniformis against 316L stainless steel. It also indicated that RTIQAS was more effective than common commercial biocides THPS (tetrakis hydroxymethyl phosphonium sulfate) and glutaraldehyde at the same concentration against X80 carbon steel corrosion by D. vulgaris. In the molecular simulation, the interaction energy confirmed the favorable adsorption of RTIQAS on the metal surfaces, indicating its potential as an effective corrosion inhibitor.

The development of chrome free chestnut and Tetrakis Hydroxymethyl Phosphonium Sulfate based Eco-benign combination tanning system

The replacement of the conventional chromium tanning system with an eco-benign tanning systems has received great attention owing to stringent environmental regulations. In this specific study, a chrome-free combination tanning system based on chestnut and tetrakis (hydroxymethyl) phosphonium sulfate (THPS) was developed and presented as an effective alternative cleaner tanning technology. Processing of the tanning trials were carried out varying the percentages of chestnut as a tannage, followed by THPS as a re-tannage for process optimization. The leathers obtained were characterized for their thermal stability, grain surface properties using a scanning electron microscope, physical strength characteristics, comfort, and organoleptic properties. Finally, the environmental impact of the tanning systems was assessed through the comparative analysis of their spent liquors. The chestnut-THPS combination system tanned leathers using 20 % chestnut followed by 2 % THPS resulted in maximum shrinkage temperature of 95OC. The hydrothermal stability of the leathers tanned using this combination tanning system were found to be better than those tanned using chestnut and THPS tanning systems alone, respectively. The strength and comfort properties of the leathers produced using the developed combination tanning system were found to be on par with or better than those of conventionally tanned leathers, and the scanning electron microscopic study depicted that the grain surface of the leathers produced were observed to be free of surface deposition. The environmental impact assessment showed that the combination tanning system used resulted in a significant reduction in TS, TDS, TSS, and BOD in the wastewater. This research article has attempted and established the use of chestnut-THPS-based combination tanning systems as an effective, eco-friendly alternative tanning process technology.

Effect of Biocides Blend on Microorganisms in Produced Water

The exploration and production of crude oil lead to the production of a significant quantity of associated gas and produce water. The produce water is either reinjected into the oil well or discharged into the environment. There is therefore a need for the water to be treated before any of the aforestated operations are carried out, as it contains a significant amount of microorganisms that pose a threat to production facilities as well as the environment. This research studies the use of a combination of two types of biocides, quaternary ammonium chloride (QUAT) and tetrakis-hydroxymethyl phosphonium sulfate (THPS), at various ratios in order to determine which is most effective. The pour-plate method for total viable bacterial enumeration was applied. It was discovered that, generally, the blends of biocide proved to be more effective in combating bacterial activities in produce water at lower concentrations and longer reaction times than the individual biocides. Thus, the 75% THPS + 25% QUAT as well as the 50% THPS + 50% QUAT were most effective at a concentration of 1000 ppm and a reaction time of 9 hrs in reducing the microbial population from 1.53 ×108 bacterial cells/ml to 3.3×102 bacterial cells/ml and 3.7×102 bacterial cells/ml, respectively.

Effects of different water quality regulators on growth performance, immunologic function, and domestic water quality of GIFT tilapia.

Water quality regulation is widely recognized as a highly effective strategy for disease prevention in the field of aquaculture, and it holds significant potential for the development of sustainable aquaculture. Herein, four water quality regulators, including potassium monopersulfate (KMPS), tetrakis hydroxymethyl phosphonium sulfate (THPS), bacillus subtilis (BS), and chitosan (CS), were added to the culture water of Oreochromis niloticus (GIFT tilapia) every seven days. Subsequently, the effects of these four water quality regulators on GIFT tilapia were comprehensively evaluated by measuring the water quality index of daily growth-related performance and immune indexes of GIFT tilapia. The findings indicated that implementing the four water quality regulators resulted in a decrease in the content of ammonia nitrogen, active phosphate, nitrite, total organic carbon (TOC), and chemical oxygen demand (COD) in the water. Additionally, these regulators were found to maintain dissolved oxygen (DO) levels and pH of the water effectively. Furthermore, using these regulators demonstrated positive effects on various physiological parameters of GIFT tilapia, including improvements in final body weight, weight gain rate (WGR), specific growth rate (SGR), condition factor (CF), feed conversion ratio (FCR), spleen index (SI), hepato-somatic index (HSI), immune cell count, the activity of antioxidant-related enzymes (Nitric oxide, NO and Superoxide dismutase, SOD), and mRNA expression levels of immunity-related factors (Tumor Necrosis Factor-alpha, TNF-α and Interleukin-1 beta, IL-1β) in the liver and spleen. Notably, the most significant improvements were observed in the groups treated with the BS and CS water quality regulators. Moreover, BS and CS groups exhibited significantly higher serum levels of albumin (ALB) and total protein (TP) (P < 0.05), whereas the other indicators showed no significant difference (P > 0.05) compared to the control group. However, the KMPS and THPS groups of GIFT tilapia exhibited significantly higher serum levels of aspartate aminotransferase (AST), alanine transaminase (ALT), creatinine (CRE) and blood urea nitrogen (BUN) (P < 0.05), whereas they exhibited significantly decreased HSI (P < 0.05). In addition, the partially pathological observations revealed the presence of cell vacuolation, nuclear shrinkage, and pyknosis within the liver. In conclusion, these four water quality regulators, mainly BS and CS, could improve the growth performance and immunity of GIFT tilapia to varying degrees by regulating the water quality and then further increasing the expression levels of immune-related factors or the activity of antioxidant-related enzymes of GIFT tilapia. On the contrary, the prolonged use of KMPS and THPS may gradually diminish their growth-enhancing properties and potentially hinder the growth of GIFT tilapia.

Flow-through electrochemical organophosphorus degradation and phosphorus recovery: The essential role of chlorine radical

Phosphorus scarcity and the deleterious ecological impact of the release of organophosphorus pesticides have emerged as critical global issues. Previous research has shown the ability of electrochemistry to induce the precipitation of calcium phosphate from phosphorus-laden wastewater to recover the phosphorus. The current study presents a flow-through electrochemical system consisting of a column-shaped electrochemical reactor, a tubular stainless-steel (SS) cathode, and a titanium suboxides (TiSO) anode. This system simultaneously oxidizes tetrakis (hydroxymethyl) phosphonium sulfate (THPS) and recycles phosphates. The influence of current density, flow rate, and initial calcium ions concentration were examined under continuous flow operation. To enhance the electrochemical reactor's performance, we elevated the current density from 5 to 30 mA cm−2, which caused the phosphorus recovery efficiency to increase from 37% to 72% within 120 min, accompanied by an enhancement of the THPS mineralization efficiency from 57% to 90%. These improvements were likely due to the higher yield of reactive species chloride species (Cl•) formed at the TiSO anode and the higher local pH at the cathode. By investigating the formation of Cl• at the TiSO anode, we found that THPS mineralization exceeded 75% in the presence of NaCl at a current density of 20 mA cm−2. The demonstrated performance of the flow-through electrochemical system should enable the utilization of anodic oxidation-cathodic precipitation for the recovery of phosphorus from organophosphorus-contaminated wastewater.

Mitigation of EH36 ship steel biocorrosion using an antimicrobial peptide as a green biocide enhancer

In this study, a 13-mer antimicrobial peptide (RRWRIVVIRVRRC) named by E6 was used as an enhancer of a green biocide to mitigate the biocorrosion of EH36 ship steel. Results show that a low concentration of E6 (100 nM) alone was no-biocidal and could not resist the Desulfovibrio vulgaris adhesion on the EH36 steel surface. However, E6 enhanced the bactericidal effect of tetrakis hydroxymethyl phosphonium sulfate (THPS). When E6 and THPS were both added to the bacteria and steel system, both the sessile D. vulgaris cells and biocorrosion rate of EH36 steel decreased significantly. Compared with the 80 ppm THPS alone treatment, the combination of 100 nM E6 + 80 ppm THPS led to an extra 1.6-log reduction in the sessile cell count. Fewer sessile D. vulgaris cells led to a lower extracellular electron transfer (EET) rate, directly resulting in 78% and 83% decreases in weight loss and pit depth of EH36 steel, respectively. E6 saved more than 50% of THPS dosage in this work to achieve a similar biocorrosion mitigation effect on EH36 steel.

Electrochemical Assessment of Mitigation of Desulfovibrio ferrophilus IS5 Corrosion against N80 Carbon Steel and 26Cr3Mo Steel Using a Green Biocide Enhanced by a Nature-Mimicking Biofilm-Dispersing Peptide.

MIC (microbiologically influenced corrosion) is problematic in many industries, especially in the oil and gas industry. In this work, N80 carbon steel for pipelines was tested with 26Cr3Mo chromium pipeline steel for comparison in SRB (sulfate-reducing bacterium) MIC mitigation using a THPS (tetrakis hydroxymethyl phosphonium sulfate)-based commercial biocide (Biotreat 5475 with 75-80% THPS by mass). Peptide A, a nature-mimicking synthetic cyclic peptide (cys-ser-val-pro-tyr-asp-tyr-asn-trp-tyr-ser-asn-trp-cys) with biofilm dispersal ability was used as a biocide enhancer. Metal coupons covered with 3-d old Desulfovibrio ferrophilus IS5 biofilms were immersed in different biocide solutions. After 1-h treatment, 200 ppm Biotreat 5475, 200 ppm Biotreat 5475 + 200 nM (360 ppb) Peptide A, and 400 ppm Biotreat 5475 achieved 0.5-log, 1.7-log and 1.9-log reductions in sessile cell count on N80, and 0.7-log, 1.7-log, and 1.8-log on 26Cr3Mo, respectively. The addition of 200 nM Peptide A cut the THPS biocide dosage by nearly half. Biocide injection tests in electrochemical glass cells after 1 h exhibited 15%, 70%, and 72% corrosion inhibition efficiency (based on corrosion current density) on N80, and 27%, 79%, 75% on 26Cr3Mo, respectively. Linear polarization resistance and electrochemical impedance spectrometry results also indicated antimicrobial efficacies.

Mitigation of galvanized steel biocorrosion by Pseudomonas aeruginosa biofilm using a biocide enhanced by trehalase

Pseudomonas aeruginosa is a facultative bacterium that is pathogenic. It is ubiquitous in the environment including air handling systems. It causes microbiologically influenced corrosion (MIC) aerobically and anaerobically. In this work, P. aeruginosa was grown as a nitrate reducing bacterium (NRB) in Luria-Bertani medium with KNO3 at 37 °C. Trehalase, an enzyme which plays a crucial role in biofilm formation was found to enhance the treatment of P. aeruginosa biofilm and its MIC against galvanized steel by tetrakis-hydroxymethyl phosphonium sulfate (THPS) green biocide. After a 7-d incubation, 30 ppm (w/w) trehalase reduced sessile cell count by 0.8-log, and it also reduced galvanized steel weight loss by 14%, compared to 2.3-log and 39%, respectively for the 30 ppm THPS treatment. The combination of 30 ppm THPS + 30 ppm trehalase reduced sessile cell count further by 0.1-log and weight loss by 13% compared to using THPS alone. Electrochemical corrosion measurements supported weight loss results. The injection of 20 ppm riboflavin into a 3-d P. aeruginosa broth failed to accelerate the corrosion rate, suggesting that nitrate reducing P. aeruginosa MIC of galvanized steel did not belong to extracellular electron transfer-MIC, because Zn was hydrolyzed after the microbe damaged the passive film.

Simultaneously removal of phosphorous and COD for purification of organophosphorus wastewater by catalytic ozonation over CaO

Organic phosphorous wastewater is very hard to be purified which need to remove both COD and phosphorous. The treatment process of organic phosphorous wastewater is commonly complicated. In this work, CaO has been used as the catalyst and precipitant for the treatment of organophosphorus wastewater by catalytic ozonation. Compared with CeO2, MnO2 and MgO, CaO shows excellent catalytic performance for the removal of COD. Additionally, the generated PO33− can be removed by Ca2+. Ca2+ can catalyze the reaction between O3 and H2O to generate active oxygen species, such as •OH, -•O2, 1O2. Besides, various conditions, namely, amount of catalyst, O3 flow rate, substrate concentration and pH for the degradation of Tetrakis Hydroxymethyl Phosphonium Sulfate (THPS) wastewater by catalytic ozonation over CaO have been optimized to determine the optimal parameter conditions. Finally, the excess Ca2+ in the solution can be removed by the addition of CO2.

Mitigation of carbon steel biocorrosion using a green biocide enhanced by a nature-mimicking anti-biofilm peptide in a flow loop

Biocorrosion, also called microbiologically influenced corrosion (MIC), is a common operational threat to many industrial processes. It threatens carbon steel, stainless steel and many other metals. In the bioprocessing industry, reactor vessels in biomass processing and bioleaching are prone to MIC. MIC is caused by biofilms. The formation and morphology of biofilms can be impacted by fluid flow. Fluid velocity affects biocide distribution and MIC. Thus, assessing the efficacy of a biocide for the mitigation of MIC under flow condition is desired before a field trial. In this work, a benchtop closed flow loop bioreactor design was used to investigate the biocide mitigation of MIC of C1018 carbon steel at 25 °C for 7 days using enriched artificial seawater. An oilfield biofilm consortium was analyzed using metagenomics. The biofilm consortium was grown anaerobically in the flow loop which had a holding vessel for the culture medium and a chamber to hold C1018 carbon steel coupons. Peptide A (codename) was a chemically synthesized cyclic 14-mer (cys-ser-val-pro-tyr-asp-tyr-asn-trp-tyr-ser-asn-trp-cys) with its core 12-mer sequence originated from a biofilm dispersing protein secreted by a sea anemone which possesses a biofilm-free exterior. It was used as a biocide enhancer. The combination of 50 ppm (w/w) THPS (tetrakis hydroxymethyl phosphonium sulfate) biocide + 100 nM (180 ppb by mass) Peptide A resulted in extra 1-log reduction in the sulfate reducing bacteria (SRB) sessile cell count and the acid producing bacteria (APB) sessile cell count compared to 50 ppm THPS alone treatment. Furthermore, with the enhancement of 100 nM Peptide A, extra 44% reduction in weight loss and 36% abatement in corrosion pit depth were achieved compared to 50 ppm THPS alone treatment.Graphical

Optimized O3/Fe(II) Using Response Surface Methodology for Organic Phosphorus Removal in Tetrakis(hydroxymethyl)phosphonium Sulfate Wastewater

Tetrakis(hydroxymethyl)phosphonium sulfate (THPS) wastewater is a kind of industrial wastewater which is difficult to biodegrade. In this work, O3/Fe(II) was used to remove organic phosphorus from THPS wastewater. The operating conditions in this process were optimized using the Box-Behnken response surface method based on single-factor experimentation. A response model of the organic phosphorus removal rate considering the initial pH, reaction time, ozone concentration, and Fe(II) dosage was established. The results showed that the ozone concentration and initial pH had a significant effect on the removal rate of organic phosphorus, and the model fit well (R2 = 0.98). The maximum removal rate of organic phosphorus predicted by this model was 86.04%, while the deviation between the predicted and experimental values was 0.91%. We concluded that the quadratic model was an effective tool for optimizing the removal of organic phosphorus in the THPS wastewater by O3/Fe(II).

Mitigation of sulfate reducing Desulfovibrio ferrophilus microbiologically influenced corrosion of X80 using THPS biocide enhanced by Peptide A

• Peptide A is a non-biocidal biofilm dispersal agent for biocide enhancement. • 20 ppm (w/w) THPS is insufficient to mitigate D. ferrophilus MIC of X80 steel. • 20 ppm THPS is enhanced by 100 ppb Peptide A, achieving 83% lower weight loss. • It also achieves 4-log extra sessile cell reduction compared with 20 ppm THPS. • 20 ppm THPS + 100 ppb Peptide A weight loss is similar to that for 50 ppm THPS. Tetrakis hydroxymethyl phosphonium sulfate (THPS) was enhanced by a 14-mer Peptide A, with its core 12-mer sequence mimicking part of Equinatoxin II protein, in the mitigation of sulfate reducing Desulfovibrio ferrophilus MIC (microbiologically influenced corrosion) of X80 carbon steel. Results proved that 50 ppm (w/w) THPS was sufficient to mitigate the D. ferrophilus biofilm, and its very agressive MIC (19.7 mg/cm 2 in 7 days or 1.31 mm/a), but not 20 ppm THPS. To achieve effective mitigation at a low dosage of THPS, biofilm-dispersing Peptide A was added to 20 ppm THPS in the culture medium. Sessile cell counts were reduced by 2-log and 4-log after enhancement by 10 ppb and 100 ppb Peptide A, respectively. Enhancement efficiency (further reduction in corrosion rate) reached 69% for 10 ppb Peptide A and 83% for 100 ppb Peptide A compared with 20 ppm THPS alone treatment, indicating that Peptide A was a good biocide enhancer for THPS.