The aim of the study was to establish the prevalence of strains of microorganisms resistant to antibacterial drugs isolated from subgingival dental plaques of periodontitis patients in the acute and remission stages. Materials and methods of research։ For the study, were recruited 90 patients who were diagnosed with chronic periodontitis (K05.3) because of clinical examination and subsequent treatment. The research methods included culture (disco-diffusion method) and molecular biological technologies (PCR), which allows identifying resistant strains of bacteria isolated from subgingival biofilm. Results։ Our results confirm that periodontopathogenic anaerobic bacteria, in contrast to sanitary-significant microbes of the ESCAPE pathogen group, were not found to be resistant to doxycycline and ciprofloxacin in the phenotypic test (disk method). The data obtained regarding the detection of genetic markers of resistance to betalactam antibiotics are generally consistent with the results of studies obtained in Western Europe (Spain, Italy, Greece). At the same time, in relation to genetic markers of resistance to tetracyclines and fluoroquinolones, significantly lower levels of detection of resistant strains were established than in studies by foreign authors. Perhaps this is due to the widespread use of these drugs in these years. On the other hand, closer results for tetracycline and fluoroquinolone resistance markers are from researchers in the People's Republic of China. Conclusion։ The results obtained allow us to justify the return to treatment regimens using doxycycline, as one of the most adequate domestic tetracycline drugs.