Direct Test Research Articles

Study on the Interface Reinforcement Effect of Bamboo Grid in Filled Loess Embankment in a High and Steep Gully

A bamboo grid is a new type of reinforcement material, which can replace traditional geogrids in the reinforcement of embankments. In this study, the reinforcement effect of the bamboo grid that is only set at the interface between the filled and undisturbed soils of the filled loess embankment in a high and steep gully was investigated. The influence of reinforcement position and grid spacing on the reinforcement effect was studied by carrying out a large-scale direct shear test, numerical simulation, and field measurement. The results indicated that the bamboo grid could enhance the shear strength of the reinforcement interface. The interface shear strength first improved and then decreased with the decrease in grid spacing. The differential settlement was significantly reduced after the reinforcement of the bamboo grid at the interface. Compared with other reinforcement positions, setting the bamboo grid in the upper part of the embankment was the most efficient and economical. When the grid spacing became dense, the reinforcement effect was improved as the differential settlement decreased. However, the improvement in the reinforcement effect by decreasing the grid spacing was limited, which meant there was an optimal grid spacing.

Emerin preserves stem cell survival through maintenance of centrosome and nuclear lamina structure.

Drosophila female germline stem cells (GSCs) complete asymmetric mitosis in the presence of an intact, but permeable, nuclear envelope and nuclear lamina (NL). This asymmetric division requires a modified centrosome cycle, wherein mitotic centrosomes with mature pericentriolar material (PCM) embed in the NL and interphase centrosomes with reduced PCM leave the NL. This centrosome cycle requires emerin, a NL protein critical for GSC survival and germ cell differentiation. In emerin mutants, interphase GSCs centrosomes retain excess PCM, remain embedded in the NL and nucleate microtubule asters at positions of NL distortion. Here, we address contributions of abnormal interphase centrosomes to GSC loss. Remarkably, reducing interphase PCM in emerin mutants rescues GSC survival and partially restores germ cell differentiation. Direct tests of effects of abnormal centrosomes were achieved by expression of constitutively active Polo kinase that drove enlargement of interphase centrosomes in wild type GSCs. Notably, these conditions failed to alter NL structure or decrease GSC survival. However, coupling enlarged interphase centrosomes with nuclear distortion promoted GSC loss. These studies establish that emerin maintains centrosome structure to preserve stem cell survival.

A 3D DEM modeling of soil-rock mixture considering spatial distribution orientation of blocks

Spatial distribution orientations of blocks can cause significant errors in the discrete element model (DEM) calculation of soil-rock mixture (SRM). To avoid this error, spherical harmonic (SH) series whose harmonization degrees fixed at 15 were proposed for block reconstruction. This research refers to the case-history of a deep excavation rift valley spanning from the Mabian to Zhaojue section of the Leshan-Xichang Expressway, mainly containing moderately-weathered silty mudstone, in the Leshan City, Sichuan Province, China. The appropriate degree of finite-term SH series is selected by the volume, surface area. 100 blocks were scanned on site, and sphericity and angularity of the blocks were calculated. The sphericity and angularity of 50 reconstructed blocks were considered for the error analysis of SH method. Moreover, stochastic polyhedron method was considered for comparing different block reconstructions. The maximum block placement angle was defined to control the spatial distribution orientations of the blocks. Large scale direct tests were carried. Numerical simulations of large-scale direct shear tests were conducted to study the influence of the spatial distribution orientations of the blocks on the mechanical properties of the SRMs. The results revealed that the finite-term SH series fixed at 15 accurately reflected the shape characteristics and mechanical behaviors of actual blocks. The spatial distribution orientations of the blocks had a minimal impact on the friction angle and cohesion of SRM constructed through the SH method. The SRMs developed via the SH method exhibited marginal variations in contact force and anisotropy index of contact across diverse block placement strategies. The evolution of coordination number was closer when employing the SH method under varied block placement methods. Blocks reconstructed by the SH method, could mitigate errors in DEM calculation caused by the spatial distribution orientations of the blocks.

Stabilization strategy of normal and chrome tanning effluent mixed subsoil: a novelty to enhance the soil characteristics

Soil stabilisation is a unique method to effectively and accurately find a solution to the issues caused by loose subsoil. This research investigates the problems of uncontrolled industrial effluent disposal, mainly from tannery enterprises, which presents severe environmental problems in emerging nations due to soil properties changing and large regions being unfit for cultivation and human habitation. The study uses Atterberg's limits, sieve analysis, and direct shear testing to examine the effects of untreated tannery effluents on soil parameters. The findings show that the soil's shear strength, moisture content, and flexibility have all significantly decreased. Lime and waste stone powder were added to lessen these impacts, and this improved soil stability was shown by an increase in the values of all the variables. For efficient soil remediation, the study suggests using waste stone powder and lime in the following proportions: 0%, 3%, 6%, and 9%. Combining lime stabilisation methods with industrial by-products like slag and fly ash opens up new possibilities for enhancing the geotechnical characteristics of polluted soils. This study emphasises how important it is to use customised geotechnical design techniques to handle soil contamination issues in infrastructure and foundation projects, especially in areas where the leather industry predominates.

Identification of immune traits associated with neurodevelopmental disorders by two-sample Mendelian randomization analysis

BackgroundOne of the main causes of health-related issues in children is neurodevelopmental disorders (NDDs), which include attention-deficit hyperactivity disorder (ADHD), autism spectrum disorder (ASD), and Tourette syndrome (TS). Nonetheless, there is relatively little prior research looking at the link between immunological inflammation and NDDs. Our work uses a two-sample Mendelian Randomization (MR) approach to provide a thorough evaluation of the causal effects of immune traits on ADHD, ASD, and TS.MethodsAs exposures, 731 immunological traits’ genetic associations were chosen, and the outcomes were genome-wide association data for ADHD, ASD, and TS. The inverse-variance weighted (IVW), weighted median (WM), and MR-Egger methods were used to conduct MR analysis. The results’ robustness, heterogeneity, and horizontal pleiotropy were confirmed using extensive sensitivity analysis.ResultsWith single-nucleotide polymorphisms serving as instruments and false discovery rate (FDR) correction applied, the study found that significantly higher expression of CD62L on CD62L+ myeloid DC (IVW, OR: 0.926, 95% CI 0.896~0.958, P = 9.42 × 10–6, FDR = 0.007) and suggestively higher absolute cell count (AC) of CD28 + DN (CD4-CD8-) (IVW, OR: 0.852, 95% CI = 0.780 ∼ 0.932, P-value = 4.65 × 10−4, FDR = 0.170) was associated with a lower risk of ADHD. There was no pleiotropy, and the causal relationships were strong according to sensitivity, leave-one-out, and MR-Steiger directionality tests. For ASD and TS, no harmful or protective immune traits were observed.ConclusionsThe results of the study lend credence to the theory that deficiency in CD62L on CD62L+ myeloid DC and CD28 + DN (CD4-CD8) AC may contribute to the onset of ADHD.

Experimental Study on Direct Shear Behavior of New-to-Old Interface for Recycled Aggregate Concrete with Different Replacement Ratios

The large amount of construction waste produced by the construction industry brings severe natural resource consumption and environmental pollution, elevating the awareness of sustainable development. Recycled aggregate concrete, crushed from construction waste, is recognized as an eco-friendly material and the current optimal solution for the environmental problem. The interface shear failure between substrate recycled coarse aggregate concrete (RAC) and overlay natural aggregate concrete becomes vital for structural safety. In order to study the direct shear performance, a total of 19 specimens were designed to carry out the direct shear test with different replacement ratios, interface types and curing ages. The whole process and failure mode were recorded. The relationships between the shear capacity and deformation, ductility, damage evolution, and energy dissipation were evaluated and discussed. The results demonstrate that the RAC component in the interface was the weak line in the shear plane, accelerating the damage evolution. In the early curing stage, the replacement ratio had a slight influence on the shear properties. Increasing the recycled aggregate replacement ratio from 0 to 100% improved the normalized strength ratio by 8.94% and 14.62%, respectively, for curing ages of 3 days and 7 days. A regression equation was proposed to predict the shear strength, accounting for the curing ages. With the increase in the replacement ratio, the ductility and energy dissipation gradually enhanced.

Mesoscopic shear evolution characteristics of frozen soil-concrete interface

The mechanical properties of frozen-concrete interfaces affect the stability and durability of engineering structures in cold regions. To investigate these properties, laboratory tests and numerical simulations were conducted to study the mesoscopic evolution of the shear stress-displacement relationship and the shearing process at the interface. The direct shear tests were performed at different environmental temperatures (−2 °C, −5 °C, and −10 °C) and normal stresses (100 kPa, 200 kPa, and 300 kPa) on the frozen soil-concrete interface, and Particle Flow Code (PFC) model of direct shear was developed. The mesoscopic parameters (particle displacement, rotation, force chain, stress, coordination number, porosity, fabric, etc.) of the interface during shearing were simulated using the PFC model. Moreover, the relationship among the interface temperature, cohesion, and friction coefficient was determined based on experimental data, and the accuracy of the PFC model was verified using previous experimental data. The results of the PFC shear model aligned well with those of the laboratory test, and the formation of shear bands was simulated well. The displacement of the soil particles on the upper layer outside the shear zone was uniform, and the direction was the same, whereas the particles inside the shear zone showed significant differences in the dislocation and rotation of the soil particles. The force chain, stress field, coordination number, and porosity were similar in the shear process and showed a concentrated distribution in the opposite direction of the shear motion, which reflected the consistency of the microcosmic response of the particles under the action of macroscopic external forces. The regression equations for the temperature, cohesion, and friction coefficient in this study can be used to simulate the shear behavior of frozen soil-concrete interfaces under different temperatures and normal stresses.

Combination of Implementation of Antiphase and Overcurrent Relays for Protection of 0.75 kW Three-Phase Induction Motor in Industry

Three-phase induction motors are the most widely used type of motor in industry. These motor operations are generally susceptible to unbalanced voltage disturbances. This interference is caused by the loss of one or two phases on the input side of the motor. This source voltage disturbance then causes an unbalanced current which flows to the coil, causing a phase shift, overheating the coil, and if left for a long time, it causes the motor to burn out. The research aims to design and implement a combination of antiphase relay (APR) and overcurrent relay (OCR) to prevent voltage drops due to failure of one or two phases and overcurrent to prevent overheating and fires in three-phase induction motor coils. Motor testing is carried out by adjusting the magnitude of the three-phase motor source voltage in three testing stages, i.e., tests A, B, and C, under no-fault conditions, 1-phase open and 2-phase open conditions respectively. The direct test results are then validated with ETAP simulations. In direct testing, A, B, and C can produce a current and relay working time of 6 A-31.04 s, 7.1 A-20.98 s, and 8.3 A-14.93, respectively. Meanwhile, testing using ETAP simulation can produce current and relay working times of 6A-31.366 s, 7A-20.599 s, and 8A-14.822 s respectively. The combination of an APR and an OCR can detect and interrupt voltage drops and overload currents in systems connected to 0.75 kW three-phase induction motors in the industry.

Global and Local Shear Behavior of the Frozen Soil–Concrete Interface: Effects of Temperature, Water Content, Normal Stress, and Shear Rate

The interface between soil and concrete in cold climates has a significant effect on the structural integrity of embedded structures, including piles, liners, and others. In this study, a novel temperature control system was employed to conduct direct shear tests on this interface. The test conditions included normal stress (25 to 100 kPa), temperature (ranging from 20 to −6 °C), water content (from 10 to 19%), and shear rates (0.1 to 1.2 mm/min). Simultaneously, the deformation process of the interface was continuously photographed using a modified visual shear box, and the non-uniform deformation mechanism of the interface was analyzed by combining digital image correlation (DIC) technology with the photographic data. The findings revealed that the shear stress–shear displacement curves did not exhibit a discernible peak strength at elevated temperatures, indicating deformation behavior characterized by strain hardening. In the frozen state, however, the deformation softened, and the interfacial ice bonding strength exhibited a positive correlation with decreasing temperature. When the initial water content was 16% and the normal stress was 100 kPa, the peak shear strength increased significantly from 99.9 kPa to 182.9 kPa as the test temperature dropped from 20 °C to −6 °C. Both shear rate and temperature were found to have a marked effect on the peak shear strength, with interface cohesion being the principal factor contributing to this phenomenon. At a shear rate of 0.1 mm/min, the curve showed hardening characteristics, but at other shear rates, the curves exhibited strain-softening behavior, with the softening becoming more pronounced as shear rates increased and temperatures decreased. Due to the refreezing of interfacial ice, the residual shear strength increased in proportion to the reduction in shear rate. On a mesoscopic level, it was evident that the displacement of soil particles near the interface exhibited more pronounced changes. At lower shear rates, the phenomenon of interfacial refreezing became apparent, as evidenced by the periodic changes in interfacial granular displacement at the interface.

Acacetin alleviates rheumatoid arthritis by targeting HSP90 ATPase domain to promote COX-2 degradation

BackgroundInflammation plays a significant role in initiating and sustaining rheumatoid arthritis (RA). Acacetin, a natural flavonoid compound, exhibits excellent anti-inflammatory effects specifically for RA. However, its relevant targets and molecular mechanisms remain to be elucidated. PurposeThis study aims to investigate the mechanism of acacetin in the therapeutic efficacy of acacetin in RA and search for new therapeutic options for RA treatment. MethodsA collagen-induced RA mouse model was established to evaluate the therapeutic effect of acacetin. Acacetin functional probes were synthesized to capture potential target proteins in RAW264.7 cells. Various small molecule-protein interaction methods were conducted to verify the binding of acacetin to target protein. Molecular docking and site directed mutagenesis tests were performed to analyze the specific binding sites. Co-immunoprecipitation, immunofluorescence assay and western blot were engineered to explore the effect of acacetin on COX-2 degradation by targeting HSP90. ResultsAcacetin specifically binds to the ATP domain of HSP90, to facilitate the dissociation between HSP90 and COX-2, inducing the ubiquitin-degradation of COX-2 in macrophages. Acacetin suppressed the production of pro-inflammatory cytokines, as well as inflammatory related pathways, exerting excellent anti-inflammatory effects in RA. ConclusionsThis research proved that acacetin, a novel HSP90 ATPase inhibitor, inhibits the functional folding of the client protein COX-2, promoting its ubiquitin degradation for anti-inflammation. Targeting HSP90 is a viable strategy to inhibit inflammation, affording a distinct way to managing joint inflammation and pains associated with RA.

Serological and molecular analysis of Leishmania infection in a recent outbreak of visceral leishmaniasis in South Omo Zone, Ethiopia.

Ethiopia has a high burden of visceral leishmaniasis. Recently, there was a significant increase in cases in the South Omo Zone. This study aims to assess the prevalence of Leishmania donovani infection and its associated factors. A household-based cross-sectional study was carried out in January 2023 in the South Omo Zone in Ethiopia. Dried blood spot samples were collected from 382 randomly selected study participants. Direct agglutination test (DAT) and kinetoplast DNA real-time PCR tests were performed to detect L. donovani infection. Participants' sociodemographic, clinical and risk factors for L. donovani infection data were collected using questionnaires. Bivariate and multivariate logistic regressions were used to analyze the data. Febrile cases were checked for malaria with a multiplex PCR assay. Overall prevalence of L. donovani infection among the sampled population was 32.5% (n=124), of which 41.1% (n=51) was detected by PCR, 33.9% (n=42) by DAT and 25.0% (n=31) by both tests. The majority of the positives were from the Logira (28.2%; n=35) and Dilbayne (29.0%; n=36) villages. Participants residing in Logira (adjusted OR [AOR]: 5.80; 95% CI 1.85 to 18.15) and Dilbayne (AOR: 3.38; 95% CI 1.15 to 9.96) villages and owning cows (AOR: 2.31; 95% CI 1.03 to 5.15) showed an association with Leishmania infection. Plasmodium falciparum was detected in 3.4% (n=2) of 59 febrile participants. The prevalence of L. donovani infection in the South Omo Zone is high. Further research on the role of cows in the transmission cycle is needed to design the best strategy to control Leishmania infection in the South Omo Zone. Such interventions should focus on the Logira and Dilbayne villages, where most of the infections were identified.

Experimental and Numerical Research on Fracture Properties of Mass Concrete Under Quasi-Static and Dynamic Loading

The dynamic fracture behavior of mass concrete is crucial to the dynamic analysis and safety evaluation of concrete dams subjected to strong earthquake shocks in the framework of fracture mechanics. In the presented research, cylindrical specimens with a ring of preset cracks were cast by three-graded mass concrete, and direct tension tests were performed with two loading rates considered, i.e., 10−6/s for quasi-static loading and 10−3/s for dynamic loading. The load–crack mouth opening displacement (P-CMOD) curves were obtained, from which the fracture toughness, fracture energy, and characteristic length of the mass concrete were obtained. In this process, the influence of the eccentricity in the tests was compensated by the numerical modeling of the tests. Next, the crack propagation process of the mass concrete was modeled using the extended finite element method. From the test results, it is found that, under quasi-static loading, the crack generally propagates along the interface between the aggregates and the matrix, while, under dynamic loading, more aggregates are fractured. As compared to the case of quasi-static loading, the energy absorption capacity, fracture energy, and fracture toughness increase for dynamic loading, while the characteristic length decreases. Moreover, the numerically predicted P-CMOD curves agree reasonably well with the experimental measurements.

Determining Rock Joint Peak Shear Strength Based on GA-BP Neural Network Method

The peak shear strength of a rock joint is an important indicator in rock engineering, such as mining and sloping. Therefore, direct shear tests were conducted using an RDS-200 rock direct shear apparatus, and the related data such as normal stress, roughness, size, normal loading rate, basic friction angle, and JCS were collected. A peak shear strength prediction model for rock joints was established, by which a predicted rock joint peak shear strength can be obtained by inputting the influencing factors. Firstly, the study used the correlation analysis method to find out the correlation coefficient between the above factors and rock joint peak shear strength to provide a reference for factor selection of the peak shear strength prediction model. Then, the JRC-JCS model and four established GA-BP neural network models were studied to identify the most valuable rock joint peak shear strength prediction method. The GA-BP neural network models used a genetic algorithm to optimize the BP neural network with different input factors to predict rock joint peak shear strength, after dividing the selected data into 80% training set and 20% test set. The results show that the error of the JRC-JCS model is a little bigger, with a value of 11.2%, while the errors of the established GA-BP neural network models are smaller than 6%, which indicates that the four established GA-BP neural network models can well fit the relationship between the peak shear strength and selected input factors. Additionally, increasing the factor number of the input layer can effectively improve the prediction accuracy of the GA-BP neural network models, and the prediction accuracy of the GA-BP neural network models will be higher if factors that have higher correlation with the output results are used as input factors.

Quality Results of Cotton Fabric Coloring with Mangsi (Phyllanthus Reticulatus) Fruit Extract

The research was done to analyze the quality of the coloring result of mangsi (phyllanthus reticulatus) fruit extract as natural dyes in cotton fabric dyeing. Extraction method was carried out through maceration process using some solvents namley water, ethanol, acidic ethanol, methanol, and acidic methanol in a ratio of 1:10 with pre-mordanting and fixation using mordant alum, calcium carbonate, and ferrous sulphate. The conducted observations included color direction test, color opacity test and color fastness to soap washing test. The result of color direction was brown to yellow-orange and blue to dark blue. The value of color opacity on cotton fabrics was in the “medium” to “very opaque” color category. The resulting value of color fastness to soap washing test was in the “good” to “poor” category. The dyeing results on cotton fabric generated an even color.

Optimizing on-site testing for post-tensioned concrete bridges

The decreasing structural reliability of key infrastructure due to aging and degradation represents a significant challenge for engineers, infrastructure managers, and society in general. The concealed nature of tendons in post-tensioned concrete bridges makes the assessment of their structural health a crucial point of several national bridge inspection guidelines. This assessment is commonly performed by means of direct and/or indirect tendon testing. A widely spread approach to the design and quantification of the number of tests is the American FHWA method, also adopted by the Italian guidelines. Unfortunately, in light of its frequentist nature and the consequent negligence of the prior knowledge on the health state of the structure, this one can lead to test campaigns with redundant and non-optimized allocation of resources and time. The present article proposes a novel Bayesian perspective of the FHWA test quantification methodology that accounts for: (i) the prior knowledge in an explicit manner by means of Bayes’ theorem; (ii) the test uncertainties, as in the case of Nondestructive Testing. By means of a demonstrative application, this article displays how this novel methodology leads to an optimized number of tests whilst guaranteeing the required structural reliability and user safety levels.

On the feasibility of a predictive model of mechanical properties of AM Inconel 718 thin wallets produced by DED-LB process monitored with thermal methods

AbstractNickel-based superalloys are widely used in applications requiring resistance to high temperatures and high strain rates. Various additive manufacturing (AM) processes, such as Laser Metal Deposition (LMD), a Directed Energy Deposition (DED) process, can be used to produce these components. The quality of the components depends on the process parameters, so it is crucial to investigate the influence of each parameter and their combinations through extensive experimental campaigns. In this scenario, it would be very important to predict the mechanical properties of the produced components through the online monitoring of the process parameters using non-destructive techniques, such as thermography. The aim of this work was to explore the feasibility to predict the mechanical properties of Inconel 718 thin wallets around 10 mm produced by DED-LB, based on the extraction of suitable thermal features directly during the production. An experimental campaign analysed the effect of different process parameters (laser power, scan speed, powder flow rate, and energy density) on the mechanical properties achieved. All sample production was monitored with an infrared uncooled camera integrated with the laser head moving at the same scan speed. After the process, hardness measurements and tensile tests in both growth directions were carried out for each sample to evaluate the mechanical behaviour of the "as-built" coupons and the influence of selected process parameters. Macrographic analyses of the material structure were performed to determine the morphology of the passes and the degree of overlap between different passes and layers. Various thermal features and statistical models were considered to demonstrate the possibility of establishing a predictive model. The obtained results demonstrated the correlation between the hardness and the apparent temperature assuming a confidence level of 95%, and the possibility of predicting in this sense the final macrostructure and the mechanical behaviour of the printed material considering an empirical model with the R2 coefficient around 0.8.

Water retention characteristics and mechanical properties of vegetated biopolymer and biochar-reinforced sandy loam

Vegetation is a sustainable strategy for erosion control and slope stabilization, though its initial cultivation can be lengthy and potentially weaken soil structures. This study compared two bio-mediated ground improvement techniques, biopolymer and biochar, known for their supportive effects on vegetation growth. Additionally, a novel treatment combining biopolymer and biochar was examined for its potential in vegetated-engineering practices. Engineering performance was assessed through soil water characteristic curve, vegetation growth, direct shear testing, and rainfall simulation. The results revealed that biopolymer and biochar treatments enhanced soil water capacity but negatively impacted vegetation germination rates and shear strength of the reinforced soil, attributed to hydrogel formation and increased soil water content from irrigation. In comparison, soil reinforced with the combined method showed a promotion in the vegetation while maintaining the soil’s mechanical performance throughout the cultivation period and exhibited only minor reductions in the shear strength compared to other reinforced soils. Moreover, the new treatment showed improved soil erodibility under a majority of rainfall occasions, regardless of the vegetation coverage. This enhanced engineering performance by the new treatment is believed to be the polymerisation between the biopolymer hydrogel, biochar and soil particles.

Flow cytometry for RBC damage and complement activation

Abstract Introduction Red blood cells (RBCs) express anionic phospholipids such as phosphatidylserine (PS) in their inner cell membrane layer. As RBCs age, physiochemical changes in their cell membranes, particularly exposure of PS on the outer membrane layer, lead to phagocytosis and clearance from circulation. PS in RBCs induces activation of the complement system, increasing binding of C3 to RBCs. Thus, quantifying PS-expressing (PS+) RBCs and complement deposition on RBCs (C3+) is a potential measure of RBC injury and may improve transfusion quality when used for predicting post-transfusion RBC recovery. Our goal was to develop a flow cytometry assay that quantifies PS+ and C3+RBCs and to assess how this assay correlates with the direct antiglobulin test for C3 (C3-DAT). Methods Using the Cytoflex S flow cytometer, we measured PS+ and C3+ RBCs using anti-annexin-PE (PS+RBC) and biotin-labeled anti-C3 with streptravidin-PE-Cy5 (C3+RBC). RBCs from healthy donors (NL-RBCs) with negative direct antiglobulin tests for C3 (C3-DAT) (n = 4) were analyzed. To assess the sensitivity of the assay and correlation with C3-DATs, complement control cells (CCC; Immucor) were used as a positive control for both PS+RBCs and C3+RBCs and diluted to different concentrations by mixing CCCs with NL-RBCs. Aliquots of diluted RBCs were analyzed blinded for C3-DAT. Differences between means for PS+RBC and C3+RBC were compared using two-sample t-test, and one-way ANOVA and Tukey’s HSD test were used to determine differences in C3+RBCs among a C3-DAT range of reactivities (0+ to 3+) in diluted CCC samples. P values less than 0.05 were statistically significant. Results In healthy, DAT-negative individuals, the mean positivity for PS+RBCs was 0.28% and for C3+RBCs was 0.34%, indicating that healthy individuals have very low percentages of RBCs with PS and C3 on their cell membrane surface. In CCC diluted samples, the mean C3+RBC percentage was 1.4% in negative C3-DAT samples, 5.9% in weak+ C3-DAT samples, 28.1% in 1+ C3-DAT samples, 67.2% in 2+ C3-DAT samples, and 99.0% in 3+ C3-DAT samples. One-way ANOVA revealed a statistically significant difference in C3+RBCs amongst all C3-DAT reactivities in diluted CCC samples (P<0.001), and Tukey’s HSD test found a mean difference in C3+RBCs percentage of 26.7% between 0+ and 1+ samples (P<0.001), 39.2% between 1+ and 2+ samples (P<0.001), and 31.8% between 2+ and 3+ samples (P<0.01). Undiluted CCC samples had a mean PS+RBC percentage of 7.0% and was statistically different from the PS+RBC percentage in NL-RBCs (P<0.01). Conclusion We describe a feasible assay to measure both PS+RBCs and C3+RBCs using flow cytometry in which CCC can be used as a positive control for PS+RBCs. This assay could serve to evaluate the quality of transfused RBC units and provide insights into the role of phosphatidylserine expression and complement deposition for RBC clearance.

The Influence of Innovative Leadership and Competence on Employee Performance

Employee performance issues are classic issues that always arise in every organization. Facing increasing competition in all lines of business, every organization must prepare its human resources to be able to compete. Human resources who are able to compete are those who always provide increased performance to their organization. The purpose of this study was to determine and analyze the influence of innovative leadership and competence on employee performance at the Jember Regency Human Resources Development and Personnel Agency. This study used a quantitative research method with 117 respondents. In this study, the data analysis techniques used were descriptive analysis, validity and reliability tests, and direct hypothesis testing. The results showed that innovative leadership had a significant effect on employee performance, that competence did not have a significant effect on employee performance.

Bond behavior between the deformed steel bar and different types of cementitious grout under reversed cyclic loading

To systematically investigate the bond behavior of deformed steel bars in cementitious grout, direct pullout tests were conducted on 15 sets of specimens by considering five types of cementitious matrixes and three different loading schemes. The failure modes, bond stress-slip curves, as well as the bond degradation and cumulative energy dissipation of specimens under reversed cyclic loading, were compared and analyzed. Results indicate that splitting pull-out or pull-out failure modes were observed, and pull-out failure was the most common failure mode of the specimen (accounting for 91 %) due to the installation of stirrups and the sufficiently short anchorage length. Meanwhile, regardless of the loading scheme, the general relationship between the magnitude of maximum bond stress of specimens composed of different cementitious materials was shown as C60＞CGM-270＞CGM-300＞CGM-340＞CGM-380, while there was no consistent pattern in relative slippage. Moreover, the relationship between the degradation of the maximum bond stress and stiffness of each specimen under reversed cyclic loading was shown as CGM-380 >CGM-340 ≈CGM-300 >CGM-270 >C60. The degradation mainly occurs in the first two cycles, and then gradually stabilizes. Furthermore, the cumulative energy dissipation relationship of specimens with different materials was shown as C60 >CGM-270 >CGM-300 >CGM-340 >CGM-380 under the reversed cyclic loading. The above investigations could provide theoretical support for the evaluation of ductility and energy dissipation of concrete members strengthened with cementitious grout under earthquake actions.