Test Sample Collection Research Articles

Identification of Urinary Tract Infection Causing Bacteria from Microbial Colony Images using Deep Learning

Abstract: Diagnosis of microbiological and bacterial infections involves extensive procedures ofsample culture and microscopic examination. The diagnosis process begins with the identification of symptoms followed by collection of test samples in the form of blood,scraps of skin lesions, mucus, sputum, urine etc. The entire process of bacteria routine culture and bacteriological examination may take as long as 10 to 11 days. Due to the long time required for the standard process of species identification, high costs and need of human expertise it is beneficial to use methods that do not rely on conventional methods. In this work we propose a comprehensive Deep Learning basedapproach to identify urinary Tract Infection (UTI) causing bacteria from microbial colony images of bacteria cultured on agar plates. The approach explains the use of VGG-19 and Inception-v3 deep learning architectures, in bacteria identification evaluated on The Annotated Germs for Automated Recognition (AGAR) dataset, an image dataset of microbial colonies cultured on agar plates. The findings affirmed the significant potential of employing deep learning techniques for the identification of microbial colonies and their classification using Petri dish images.

Mengiplementasikan Vector Space Model Similarity Euclidean Distance Menggunakan TFIDF Pada Klasifikasi Teks Bahasa Indonesia

Weighting based on the term with stemming techniques to get the basic word form term in question. This will the application of the Indonesian language text classification machine using the K-Nearest Neighbor algorithm and the Vector Space Model method on the TFIDF frequency weighting of the number of words and the Euclidean Distance function. comparison between the test documents and the test sample collection Using news documents as learning documents, a total of 10 (10) documents with 3 (three) categories, produces an Precision and Recall 90.00% for k = 5 using frequency weighting in words with the Euclidean Distance function.

Development and laboratory evaluation of a lateral flow device for the detection of swine vesicular disease virus in clinical samples

A lateral flow device (LFD) for the detection of swine vesicular disease (SVD) virus (SVDV) and differential diagnosis from foot-and-mouth disease (FMD) was developed using a monoclonal antibody (Mab C70). The performance of the LFD was evaluated in the laboratory on suspensions of vesicular epithelia and cell culture passage derived supernatants of SVDV and porcine teschovirus (enterovirus; PEV). The collection of test samples included 157 which were positive for SVDV (84 vesicular epithelial suspensions and 73 cell culture antigens) from suspected cases of vesicular disease in pigs collected from 14 countries between 1966 and 2008 and 663 samples which were either shown to be negative for SVDV and FMD virus (FMDV) or else collected from healthy pigs or demonstrated to be positive for FMDV, PEV or vesicular exanthema (VEV) and collected from 16 countries between 1965 and 2008 or else were derived from experimental animals. Three further samples containing vesicular stomatitis virus (VSV) were also tested. The diagnostic sensitivity of the LFD for SVDV was similar at 82% compared to 86% obtained by the reference method of antigen ELISA, and the diagnostic specificity was 100% compared to 99.7% for the ELISA. The device recognized virus strains of each of the known genotypes of the sole SVDV serotype. Reactions with FMDV, VEV, VSV and PEV which can produce clinically indistinguishable syndromes in pigs, did not occur. These data illustrate the potential for the LFD to be used next to the animal for providing rapid and objective support to veterinarians in their clinical judgment of vesicular disease in pigs and for the specific pen-side diagnosis of SVD and differential diagnosis from FMD.

Monitoring of Occupational Exposure to Methylene Chloride: Sampling Protocol and Stability of Urine Samples*

A sampling protocol for biomonitoring of the volatile solvent methylene chloride (MeCl(2)) by analysis of urine from exposed workers was established. Storage temperature, sample volume in headspace vial (HSV), and time to sealing HSV on determination of MeCl(2) in urine were evaluated. MeCl(2) was analyzed by a solid-phase microextraction technique combined with gas chromatography. Volume of urine in HSV has no effect on MeCl(2) analysis. Delays of 30 and 60 min from collection of urine until sealing the HSV caused 14.47 +/- 6.98% and 26.17 +/- 9.57% decreases from baseline concentration, respectively. MeCl(2) concentration in spiked urine samples stored in sealed HSVs decreased on day 2 and then remained stable for 2 weeks. Refrigeration did not improve recovery although it seems to be associated with less variability. MeCl(2) in urine samples of seven exposed workers was in the range of 0.02-0.06 mg/L. Sampling of MeCl(2)-containing urine should include collection of urine in closed plastic bottles, transfer to HSV within 15 min, sealing and clamping of HSV within 15 s, and storage of HSV in refrigeration until analysis, but no longer than 2 weeks. Standard samples should be prepared on the day of test sample collection and handled under the same conditions.

Quantitative radiometric measurement of radioactive ores in the natural occurrence

The measurement of gamma radiation of radioactive ores in the natural deposit — directly in the face of a mine working or in a bore hole-permits determination of the radioactive element content in ores without collection of test samples and without chemical analyses. The accuracy of quantitative measurement by gamma rays depends to a considerable degree on correct interpretation of the spectral content of the gamma radiation measured. The gamma-ray spectra of radioactive ores is determined by the process of radiation dispersion and depends on the content of the ore. The intensity of gamma radiation registered by an ore seam depends on the cathode malarial of the counter (tungsten, copper, graphite) as a consequence of the difference in spectral sensitivity of various counters. The relationship of gamma — radiation intensity during measurement with a Geiger counter with various cathodes permits one to characterize the spectral composition of the ore's gamma radiation. In order to express the results in general units, microroentgens per hour, the radiometers are calibrated with an exact standard, but the calibration method does not exclude the radiometer reading dependence on the spectral sensitivity of the counters. In this article are determined the important conversion coefficients connecting gamma-radiation intensity with the content of radioactive elements in ore (see the table) during measurements on the ore seam surface or in borings. During measurements in borings the reverse dissemination of gamma rays having low energy is of great importance. By use of these coefficients calculations of the radioactive element content in ore from counter readings are carried out.