Abstract: This research addresses the implementation of digital signature technique for electronic prescription to prevent cybercrime problem such as robbery, modification and unauthorized access. In this research, RSA 2048-bit algorithm will be implemented in Java programming and android based system. Secure Electronic Prescription (SEP) application design is intended to combine given services, such as confidentiality, authentication, and non-repudiation. Cryptography is used to ensure the prescription file and QR-Code for detailed information on the prescription that have been given only for the pharmacist. The QR-Code will be encrypted using an asymmetric algorithm based on NIST Standard. In the application, there are two schemes, namely the protection schemes and verification scheme. This research uses black-box testing, and white-box testing to test input values, code and output without testing the process and design that occurs in the system. We demonstrate the implementation of cryptography in Secure Electronic Prescription (SEP). The implementation of digital signature in this research can prevent archive thievery which is shown on implementation and is proven on the test