Tested Gram Positive Bacteria Research Articles

CHEMICAL COMPOSITION AND BIOACTIVITIES OF ESSENTIAL OIL FROM AN ENDEMIC SALVIA ABSCONDITIFLORA GREUTER & BURDET

Objective: The study aimed to determine the chemical composition and antibacterial, antibiofilm, and anti-quorum sensing activities of the essential oil of Salvia absconditiflora Greuter & Burdet (an endemic species) growing wildly in Türkiye. Material and Method: The essential oil from the aerial parts of the plant was obtained by hydro-distillation (0.4%) and analyzed by GC-FID and GC-MS. In addition, the broth microdilution method was used to determine antibacterial activity. The crystal violet assay was performed for antibiofilm activity, and the reporter bacteria Chromobacterium violaceum ATCC 12472 was used in the anti-quorum sensing activity test. Result and Discussion: The major components of the essential oil were identified as 1,8-cineole (32.2%), camphor (13.6%), α-pinene (7.6%), camphene (5.5%), and viridiflorol (5.1%). The essential oil showed the best antibacterial activity against Gram-positive test bacteria, with a minimum inhibitory concentration (MIC) of 0.0078 (v/v) against Staphylococcus aureus strains. The percentage biofilm inhibition value of the essential oil was determined as 84.4%. The inhibition of violacein production by the essential oil in Chromobacterium violaceum ATCC 12472 indicated the possibility of anti-quorum sensing activity. The results of this study show that the essential oil of S. absconditiflora could be a promising alternative in fighting bacterial infections.

Design, synthesis and biological evaluation of novel indole-piperazine derivatives as antibacterial agents

Herein, a series of novel indole-piperazine derivatives were synthesized. Bioassay results showed the title compounds exhibited moderate to good bacteriostatic efficacy against the test Gram-positive bacteria and Gram-negative bacteria, including methicillin-resistant Staphylococcus aureus (MRSA). Among theses compounds, three remarkable compounds 8f, 9a, and 9h exhibited superior in vitro antibacterial profiles for anti- S. aureus and anti-MRSA to that of gentamicin. Hit compound 9a manifested a rapid bactericidal kinetic effect on MRSA, with no resistance observed after 19 days of sequential passaging. And 8 µg/mL of compound 9a displayed considerable post antibacterial effects to that of ciprofloxacin at the concentration of 2 µg/mL. Cytotoxic and ADMET studies indicated, to some extent, compounds 8f, 9a, and 9h were up to the standard for antibacterial drugs. These results suggest that indole/piperazine derivatives based on the title compounds can serve as a new scaffold for antimicrobial development.

Isolation of Fungi from Mangrove Ecosystem of Mumbai and Evaluation of their Antibacterial Potential

In the current study, fungi from the mangrove ecosystem of Mumbai were isolated and their metabolites were screened for antibacterial potential. Two weeks old broth and mycelium were extracted using chloroform and methanol. Antibacterial property of solvent extracts was evaluated at various concentrations (2 – 10 µg/ml) against Staphylococcus aureus, Bacillus subtilis, Enterococcus faecium, Enterococcus faecalis, Klebsiella pneumonia and Escherichia coli, by well diffusion method. Fungi isolated were identified as Aspergillus niger, Aspergillus flavus, Trichoderma harzianum, Cylindrocladium scoparium and Colletotrichum wuxiense. Results revealed that broth solvent extracts of isolates inhibited the growth of all gram-positive test bacteria, chloroform broth extract of Cylindrocladium scoparium, Colletotrichum wuxiense and ethanolic broth extract of Aspergillus flavus, Trichoderma harzianum exhibited antibacterial potential against gram negative test organisms. Chloroform and ethanol mycelium extracts of Trichoderma harzianum and Aspergillus flavus, respectively, exhibited 100% growth inhibition potential against all test organisms. The current investigation endorses the potent secondary metabolism of the identified isolates and their potential to synthesise antibacterial compounds.

Isolation, characterization and antibacterial activity of actinomycetes from soils of the Langun-Gobingob Cave Complex in Calbiga, Samar

The discovery of rare Actinomycetes from unexplored areas has proven to be a promising source of new antimicrobials. The Langun-Gobingob cave complex in Calbiga, Samar, Philippines is the second largest cave complex in Asia, yet this is the first reported search for actinomycetes conducted in the area. The main objective of this study was to assess the antibacterial activities of culturable actinomycetes from soil samples from the Langun-Gobingob cave complex. Five actinomycete colonies were isolated in pure culture from soil samples using Starch-Casein Agar medium. The isolates were identified by their morphological characteristics and they were characterized by 16S rRNA sequencing. The sequences were matched to sequences uploaded to the NCBI Basic Local Alignment Search Tool (BLAST). Four of the isolates belonged to the Genus Streptomyces and one belonged to the Genus Nocardia. The antibacterial potential of the isolates was tested against gram-positive and gram-negative test bacteria. One Streptomyces isolate exhibited antibacterial activity against the two gram-positive test bacteria. The limited cave area sampled in this study leaves the cave complex mostly unexplored in the search for antibacterial secondary metabolites.

Isolation, characterization, anti-MRSA evaluation, and in-silico multi-target anti-microbial validations of actinomycin X2 and actinomycin D produced by novel Streptomyces smyrnaeus UKAQ_23

Streptomyces smyrnaeus UKAQ_23, isolated from the mangrove-sediment, collected from Jubail,Saudi Arabia, exhibited substantial antimicrobial activity against methicillin-resistant Staphylococcus aureus (MRSA), including non-MRSA Gram-positive test bacteria. The novel isolate, under laboratory-scale conditions, produced the highest yield (561.3 ± 0.3 mg/kg fermented agar) of antimicrobial compounds in modified ISP-4 agar at pH 6.5, temperature 35 °C, inoculum 5% v/w, agar 1.5% w/v, and an incubation period of 7 days. The two major compounds, K1 and K2, were isolated from fermented medium and identified as Actinomycin X2 and Actinomycin D, respectively, based on their structural analysis. The antimicrobial screening showed that Actinomycin X2 had the highest antimicrobial activity compared to Actinomycin D, and the actinomycins-mixture (X2:D, 1:1, w/w) against MRSA and non-MRSA Gram-positive test bacteria, at 5 µg/disc concentrations. The MIC of Actinomycin X2 ranged from 1.56–12.5 µg/ml for non-MRSA and 3.125–12.5 µg/ml for MRSA test bacteria. An in-silico molecular docking demonstrated isoleucyl tRNA synthetase as the most-favored antimicrobial protein target for both actinomycins, X2 and D, while the penicillin-binding protein-1a, was the least-favorable target-protein. In conclusion, Streptomyces smyrnaeus UKAQ_23 emerged as a promising source of Actinomycin X2 with the potential to be scaled up for industrial production, which could benefit the pharmaceutical industry.

Aktivitas Antibakteri In-Vitro Ekstrak Etanol Beberapa Jenis Tanaman Krokot (Portulaca sp)

Purslane has antibacterial benefits. The diversity of purslane plants from the genus Portulaca is about 40-100 species found in tropical and four-season areas. The aim of this study was to test the in-vitro antibacterial potential of the ethanol extract of some purslane (Portulaca sp) cultivars against the tested bacteria. In-vitro antibacterial activity testing was performed using the disc diffusion method. This study used Portulaca oleracea and Portulaca grandiflora purslane cultivars. The results of the observation of the antibacterial activity testing of several cultivars of Portulaca grandiflora (K1, K3) and Portulaca oleracea (K2) against the test bacteria Staphylococcus aureus, Escherichia coli, and Pseudomonas aeruginosa were able to show antibacterial activity with various inhibition zones ranging between 1.56 cm and 2.86 cm. Based on the results of testing for antibacterial activity of several cultivars of purslane plant, it was shown that the sensitivity of the Gram-positive test bacteria Staphylococcus aureus was higher than the Gram-negative test bacteria Escherichia coli and Pseudomonas aeruginosa. Keywords—: Portulaca oleracea, Portulaca grandiflora, disc diffusion method

Synthesis and Characterization of a New PAMAM Metallodendrimer for Antimicrobial Modification of Cotton Fabric

The synthesis and spectral characterization of a new PAMAM metallodendrimer modified with four 4-hexylamino-1,8-naphthalimide units was described. The chemical structure of metallodendrimer has been analyzed with Fourier transform infrared spectroscopy, electronic, nuclear magnetic resonance spectroscopy, and scanning electron microscopy (SEM) analysis. The antimicrobial activity of PAMAM dendrimer and its Zn complex [Zn2(D)(NO3)4] was investigated in agar medium and in meat-peptone broth against several pathogenic bacteria and yeasts. The antimicrobial screening showed good inhibition activity of the compounds better expressed against the test Gram-positive bacteria and yeasts. The antimicrobial efficacy of cotton fabrics treated with each of the dendrimers was evaluated, and their anti-adhesive and biofilm inhibiting potential was analyzed by SEM. The complex [Zn2(D)(NO3)4] showed stronger antimicrobial and biofilm inhibiting abilities than those of the ligand. The cytotoxicity effect of the dendrimers was also investigated in vitro in model HEp-2 cell line.

A new monoterpene and a new sesquiterpene from the roots of Ageratina adenophora

A new thymol type monoterpene, 2α-methoxyl-3β-methyl-6-(acetyl-O-methyl)-2,3-dihydrobenzofuran (1), and a new cadinane-type sesquiterpenoid, 1,6-dihydroxy-1-isopropyl-4,7-dimethyl-3,4-dihydronaphthalen-2(1H)-one (2), were isolated from the roots of Ageratina adenophora, along with four known compounds, eupatorenone (3), 3-hydroxymuurola-4,7(11)-dien-8-one (4), 9-oxoageraphorone (5) and (4R,5S)-4-Hydroxy-5-isopropyl-2-methyl-2-cyclohexehone (6). Their structures were determined by spectroscopic methods, including nuclear magnetic resonance (NMR) and mass spectrometry (MS) techniques. These compounds were all isolated from the roots of this plant for the first time. Compounds 1, 3 and 4 showed in vitro bacteriostatic activity against three test Gram-positive bacteria with MIC values ranging from 12.5 to 50 μg/mL. While, no obvious inhibitory activity was detected for the other compounds.

Antibacterial activity of thin films TiO2 doped with Ag and Cu on Gracilicutes and Firmicutes bacteria

This article aims to explore the antibacterial activity of thin films of TiO2 doped with Ag and Cu using two types of Gram-negative and Gram-positive test bacteria with clinical significance (Gracilicutes and Firmcutes bacteria). The thin films (thickness of about 60 nm) were deposited on glass substrates by radio frequency magnetron co-sputtering (r.f. power of 50 W) of TiO2 target with Ag and Cu pieces on its surface in an Ar atmosphere (0.8 Pa) without heating during the deposition. The total surface area of the Ag was 60 mm2 and that of the Cu was 100 mm2. Bacillus cereus, Staphylococcus epidermidis, Salmonella enterica, Escherichia coli and Pseudomonas sp. were used as test strains. The antibacterial actvity of the films was evaluated by the classical Koch's method and optical density measurements. The bactericidal effect was established at different time points between 30 min and 90 min for Pseudomonas sp. and S. enterica. The Firmicutes bacteria B. cereus and S. epidermidis were killed at the 4th and 8th hour of the treatment, respectively. The effect on E. coli was bacteriostatic until the 10th hour. The results were confirmed by assessment of the bacterial dehydrogenase activity. The studied thin films of TiO2 co-doped with Ag and Cu have a potential for application as antibacterial coatings.

English

The present studies cover antibacterial activity of 15 crude extracts (5 extracts each from hexane, dichloromethane and methanol) from five ethnomedicinal plants viz. Anaphalis nepalensis, Piptanthus nepalensis, Senecio raphanifolius, Thermopsis barbata and Thermopsis inflate collected from Sagarmatha region of Nepal against one Gram-positive (Staphylococcus aureus) and three Gram-negative (Escherichia coli, Pseudomonas aureginosa and Klebsiella pneumonia) bacterial strains. Plants used to treat at least one ailment considered to be caused by bacterial infection were identified and further investigated for their antibacterial activity. Crude extracts were obtained by the successive extraction method and examined in-vitro using the agar well diffusion process. Among 15 extracts examined, 13 (86 %) extracts showed antibacterial property against Staphylococcus aureus followed by 8 (53 %) extracts against Escherichia coli and 6 (40 %) extracts each against Pseudomonas aeruginosa and Klebsiella pneumonia. Plant extracts were more likely to inhibit Grampositive bacteria, Staphylococcus aureus. With respect to Gram-negative bacteria, it was more common for plant extracts to inhibit Escherichia coli than Pseudomonas aeruginosa or Klebsiella pneumonia. Overall, 86 % of plant extracts showed activity against Gram-positive test bacteria, and 45 % showed activity against Gramnegative test bacteria. This research results will promote to search for alternative, inexpensive and simply available antibacterial of plant origin.

Molecular cloning, expression of a galectin gene in Pacific white shrimp Litopenaeus vannamei and the antibacterial activity of its recombinant protein

Galectins play crucial roles in innate immune responses in invertebrate by recognizing and eliminating microinvaders. In this study, a cDNA encoding a galectin in the Pacific white shrimp (L. vannamei) was identified and characterized. A recombinant variant of this lectin, rLvgalectin, was expressed in the model organism P. pastoris and its expression was confirmed by Western blot. Biochemical assays indicated that the recombinant protein antibacterial rLvgalectin activity and was expressed in all of the organism's tested tissues Injection of the bacterium V. alginolyticus into L. vannamei induced hemocytes upregulation of Lvgalectin. The recombinant Lvgalectin protein (rLvgalectin) could bind various microorganism including Gram-positive bacteria, Gram-negative bacteria and yeast. And it revealed antimicrobial activity against the test Gram-positive bacteria, Gram-negative bacteria, but did not inhibit the growth of fungus Pichia pastoris. Moreover, rLvgalectin could significantly enhance the clearance activity of V. alginolyticus in vivo. In vivo challenge experiments showed that the recombinant rLvgalectin protein can significantly reduce the mortalities of V. alginolyticus injection. Furthermore, Compared to their wild-type counterparts, Lvgalectin-silenced shrimp exhibited increased mortality and hemocyte apoptosis, decreased bacterial clearance ability and total hemocyte counts, and stronger expression of Lvp53, LvproPO, LvPEN3, and LvCrustin following V. alginolyticus challenge. Taken together, these results suggest that galectin is important in the innate immune response of shrimp to pathogens infection.

Antibacterial Effect of Aquatic Extract of Heracleum Spp. Hogweed Plants from Europe on Thirteen Different Bacteria

Aquatic extracts of Heracleum (family: Apiaceae (Umbelliferae)) collected from Norway and Netherlands were tested against three bacterial strains isolated from humans, three fish pathogens, four non-pathogens, and three lactic acid bacteria (LAB) isolated from fish intestine in an in vitro growth inhibition assay. Highest in vitro growth inhibition of the test bacteria was noticed in the sterile supernatant of 5 g H. persicum and 5 g H. mantegazzianum)collected from Netherlands) boiled in 500 ml water for 2 h. Generally, highest growth inhibition was observed on Gram-positive test bacteria but lesser effect was noticed on LAB isolated from fish intestine. The aqueous extracts of H. persicum showed highest activities over Bacillus megaterium, Micrococcus sp., Pseudomonas sp. and Staphylococcus aureus.

English

Crude extracts of four actinomycetes species belonging to Actinoplane [NB 034],Streptomyces [TR 007] and Actinopolyspora [NB 069 and TR 008] genera were screened for antibacterial activities against a wide range of bacterial strains. The extracts showed antibacterial activities against both Gram-negative and Gram-positive test bacteria with zones of inhibition ranging between 2 and 27 mm (NB 034); 9 and 15 mm (TR 007); 8 and13 (NB 069) and 2 and 12 mm (NB 069). The minimum inhibitory concentration (MIC) of the extracts against the test bacteria ranged from 0.039 - 5 mg/ml, while the minimum bactericidal concentrations (MBC) ranged from 5 to >10 mg/ml. The extracts exhibited concentration-time dependent killing of Staphylococcus aureus OKOH2A and Staphylococcus scuiri OKOH2B with a 0.73log10 and 0.88log10reduction in viable counts respectively, while a re-growth phenomenon was exhibited byKlebsiella pneumonia ATCC 4352 and Escherichia coli ATCC 25922 at MIC, 2 × MIC and 4 × MIC of Streptomyces (TR 007) Actinopolyspora (TR 008) extracts. All the active extracts exhibited bacteriostatic activities.   Key words: Actinoplane, Streptomyces, Actinopolyspora, antibiotic production.

Antibacterial activity of Beta vulgaris L. pomace extract

Antibacterial activity of Beta vulgaris L. (beetroot) pomace extract (concentration 100 mg/ml) was tested against five Gram positive and seven Gram negative bacterial strains (reference cultures and natural isolates). Disc diffusion method with 15 ?l of extract and agar-well diffusion method with 50 and 100 ?l were used. Antibiotic (cefotaxime/clavulanic acid) was used as a control sample. The tested extract showed the highest activity against Staphylococcus aureus and Bacillus cereus, where clear zones (without growth) appeared. There was no any activity against other tested Gram-positive bacteria, except for Staphylococcus epidermidis, with a small zone of reduced growth. Growth of all tested Gram-negative bacteria was inhibited usually with 100 ?l of extract. The most susceptible were Citrobacter freundii and Salmonella typhymurium. The tested antibiotic gave clear, usually large zones for all tested strains except for Staphylococcus cohni spp. cohni, where only a zone of reduced growth appeared.

Antibiotic Producing Potentials of Three Freshwater Actinomycetes Isolated from the Eastern Cape Province of South Africa

Crude extracts of three actinomycetes species belonging to Saccharopolyspora (TR 046 and TR 039) and Actinosynnema (TR 024) genera were screened for antibacterial activities against a panel of several bacterial strains. The extracts showed antibacterial activities against both gram-negative and gram-positive test bacteria with inhibition zones ranging from 8 to 28 mm (TR 046); 8 to15 mm (TR 039); and 10 to 13 mm (TR 024). The minimum inhibitory concentrations ranged from 0.078 to 10 mg/mL (TR 046); 5 to >10 mg/mL (TR 039); and 1.25 to 5 mg/mL (TR 024). Time-kill studies revealed that crude extract of TR 046 showed strong bactericidal activity against Bacillus pumilus (ATCC14884), reducing the bacterial load by 104 cfu/mL and 102 cfu/mL at 4× MIC and 2× MIC, respectively, after 6 h of exposure. Similarly, against Proteus vulgaris (CSIR 0030), crude extract of TR 046 achieved a 0.9log10 and 0.13log10 cfu/mL reduction at 5 mg/mL (4× MIC) and 1.25 mg/mL (2× MIC) after 12 h of exposure. The extract was however weakly bactericidal against two environmental bacterial strains (Klebsiella pneumoniae and Staphylococcus epidermidis); and against Pseudomonas aeruginosa (ATCC 19582): the extract showed bacteriostatic activities at all concentrations tested. These freshwater actinomycetes appear to have immense potential as a source of new antibacterial compound(s).

Studies on the antimicrobial activities of extracts from Eupatorium lindleyanum DC against food spoilage and food-borne pathogens

Antimicrobial activity of extracts from Eupatorium lindleyanum DC (EEL), a traditional Chinese medicine, was screened against eight selected food spoilage and food-borne pathogens. The EEL was observed to show effective antimicrobial effects with a remarkable dose–response relationship and broad antimicrobial spectrum on all test Gram-positive bacteria and Gram-negative species. Acid environment or medium helps enhance the antimicrobial activity. The activity remains rather stable under conventional food sterilization conditions and normal shelving temperature. The EEL obtained by water decoction exhibited the maximum inhibitory effect on test strains with its MIC (minimum inhibitory concentration) and/or MBC (minimum bactericidal concentration) value of 0.4–0.8 mg ml −1. When the EEL was used in commercial orange juice at a concentration of 0.4 mg ml −1, a similar antimicrobial effect to potassium sorbate at the level of 0.2 mg ml −1 was observed. In conclusion, in addition to use as a functional food ingredient, the EEL may be selected as an inhibitor to preserve food products where a natural preservative is desired.

In vitro biological activities of South African Pelargonium (Geraniaceae) species

Despite commercial interest and ethnobotanical data, the pharmacological activities of a number of indigenous Pelargonium species hitherto remain poorly explored. The acetone extracts of twenty-one Pelargonium species (section Pelargonium) were included in this study. Using the 2,2-diphenyl-1-picrylhydrazyl (DPPH) assay, potent anti-oxidant activity was observed for the crude extracts of P. betulinum and P. crispum (IC 50 values of 4.13 ± 0.14 μg/ml and 4.49 ± 0.18 μg/ml, respectively compared to ascorbic acid, IC 50 = 4.72 ± 0.14 μg/ml). The antimicrobial (both bacterial and fungal) potential of the extracts was evaluated by using the minimum inhibitory assay. The crude extracts of P. glutinosum (SBG), P. pseudoglutinosum, P. scabrum and P. sublignosum exhibited considerable antimicrobial activity against the Gram-positive test bacteria, with P. pseudoglutinosum demonstrating the highest inhibitory activity (MIC = 39 μg/ml); however the aforementioned species were also relatively cytotoxic. The 3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyltetrazolium bromide (MTT) cellular viability assay was used to determine the toxicity of the acetone extracts against transformed human kidney epithelial (Graham) cells. The acetone extracts of P. sublignosum and P. citronellum (NBG) displayed the highest toxicities (IC 50 = 11.89 ± 1.54 μg/ml and 19.14 ± 0.98 μg/ml, respectively). The results from the toxicity assay suggested that the antimicrobial activity of the extracts may be ascribed to general cytotoxic effects. Considering all the results collectively, Pelargonium cordifolium appears to be the most promising species requiring further investigation.

Pumilicin 4, A Novel Bacteriocin with Anti-MRSA and Anti-VRE Activity Produced by Newly Isolated Bacteria Bacillus pumilus Strain WAPB4

A total of 34 bacterial strains with anti-methicillin-resistant Staphylococcus aureus (MRSA) activity were isolated from 69 soil and water samples collected from four areas of Thailand. One strain, WAPB4 identified as Bacillus pumilus, showed remarkable antibacterial activity against MRSA, vancomycin-resistant Enterococcus faecalis (VRE), and several Gram-positive test bacteria. Bacteriocin produced by WAPB4 was designated as pumilicin 4. It was heat stable up to 121 degrees C, 15 min and active within the pH range of 3-9. Its activity disappeared when treated with pronase E, chymotrypsin, and trypsin, demonstrating its proteinaceous nature. At high dosage (80 AU mL(-1)), the effect of pumilicin 4 was bactericidal to both MRSA and VRE. Bacteriostasis was observed for a low dose of bacteriocin (20 AU mL(-1)). Purification of pumilicin 4 was performed by a three-step procedure, i.e., solvent extraction, solid phase extraction, and reversed-phase chromatography. The molecular mass of purified pumilicin 4 as determined by mass spectrometry was 1994.62 Dalton. This present study is the first report of a novel bacteriocin, pumilicin 4, produced by B. pumilus that has potential for use as an alternative antibacterial agent for the treatment of infection with MRSA and VRE.

Antagonistic and antimicrobial activities of some bacterial isolates collected from soil samples

Thirty seven bacterial cultures isolated from soil samples obtained from different locations were tested for their antagonistic activity against some fungal pathogens, viz., Sclerotium rolfsii, Fusarium oxysporum and Rhizoctonia solani, causal agents of collar rot of sunflower, wilts and root rots, respectively. Among them, 5 bacterial strains, viz., A1 6 (Bacillus sphaericus), K1 24 (Pseudomonas fluorescens), M1 42 (Bacillus circulans), M1 66 (Bacillus brevis) and T1 22 (Bacillus brevis) showed positive antagonistic activity. M1 66 was the most effective in inhibiting mycelial growth of S. rolfsii in vitro followed by M1 42, T1 22, K1 24 and A1 6. Only one bacterial strain i.e. M1 42 exhibited antagonistic activity against F. oxysporum, and none of the bacterial strains gave positive activity against R. solani. Furthermore, antimicrobial activities of all the 5 strains were checked against different test organisms. These strains showed their extensive inhibition effect particularly against gram-positive test bacteria (Staphylococcus aureus and Bacillus subtilis) and the test fungal strain (Candida albicans). On the other hand, B. brevis M1 66 and B. brevis T1 22 strains had an inhibitory effect against gram positive and gram-negative test bacteria (Escherichia coli and Proteus vulgaris) as well as the test fungal strain.

Antibacterial and Antioxidant Activity and Essential Oil Composition of Grammosciadium scabridum Boiss. from Iran

The in vitro antibacterial and antioxidant activity of the essential oil and its two main components of Grammosciadium scabridum Boiss. (Apiaceae) growing wild in Iran, as well as the composition of its essential oil were studied. A total of 19 compounds representing 99.9% of the oil has been identified. Gamma-Terpinene (73.5%), p-cymene (14.2%) and (E)-beta-farnesene (5.3%) were characterized as the main components. The oil showed remarkable activity against three Gram-negative and four Gram-positive test bacteria, with minimal inhibitory concentration (MIC) values ranging from 0.31 to 10.00 mg/ml. The oil and its two main components were also subjected to screening for their possible antioxidant activity by using the 2,2-diphenyl-l-picrylhydrazyl (DPPH) assay. The free radical scavenging capacity of the oil was determined with an IC50 value of 6.6 mg/ml.