Alternative Test Research Articles

Magnetic resonance imaging in patients with cardiac implantable electronic devices: the RESONANCE Spanish registry.

Despite increasing evidence demonstrating the safety of magnetic resonance imaging (MRI) in patients with cardiac implantable electronic devices (CIEDs), this procedure is often neglected in this population. This Spanish registry aimed to determine the proportion of MRI referrals and performance among patients with pacemakers (PMs) or implantable cardioverter-defibrillators (ICDs). This prospective, multicenter, open-label registry involved 21 Spanish centers. Data were collected upon implant of PMs or ICDs from BIOTRONIK and one year after, and included the number of MRIs and computed tomography scans prescribed, performed and denied, and reasons for denial. Data from 1105 patients (mean age: 74.2 years) were analyzed and 982 completed the follow-up. Of them, 82.2% had a PM and 17.8% an ICD. A total of 351 imaging tests were prescribed in 220 patients (19.9%), including 52 MRIs in 39 patients (3.5%) and 299 computed tomography scans in 196 patients (17.8%). Among the MRIs, 44 (84.6%) were performed, five (9.6%) were not performed, and three (5.8%) were replaced by an alternative test. Most of the indicated computed tomography scans were performed (97.7%). The proportion of patients with an MRI scan referral was 4.6% during the pre-COVID-19 period and 2.6% during the COVID-19 period. No MRI-related arrhythmic ventricular event was reported. This registry revealed that only 3.5% of patients with CIEDs had an MRI referral over the study, with rates decreasing to 2.6% during the COVID-19 period. These rates contrast with the 85 MRIs conducted per 1000 inhabitants in Spain in 2020.

Intrathecal enalapril reduces adhesion formation in experimentally induced digital flexor tendon sheath injuries in horses.

The objectives of the study were to describe a standing percutaneous adhesion induction model in the digital flexor tendon sheath (DFTS) of horses and to evaluate the effect of intrathecal administration of the angiotensin-converting enzyme (ACE) inhibitor enalapril on tendon healing and adhesion formation. Randomized, blinded, controlled experimental study. Eight healthy horses. A collagenase-induced adhesion model was implemented in the deep digital flexor tendon (DDFT) of both forelimbs under standing ultrasonographic guidance. Daily intrathecal injections of 5 mg enalapril (the treatment condition) were administered to a randomly assigned forelimb for 5 days, with the contralateral limb receiving an equivalent volume of 0.9% NaCl (the control). Lameness and limb circumference were recorded weekly. Horses were euthanized after 8 weeks and evaluated for gross digital flexor tendon sheath (DFTS) adhesions. Tendons were collected for histopathologic scoring of DDFT healing. Paired data were analyzed using a one-sided alternative sign test and longitudinal regression. Multiple DFTS adhesions were formed in control limbs of all horses. The median number of gross DFTS adhesions in treated limbs was less than in control limbs (p = .0039). The average reduction in limb circumference and lameness scores over time occurred faster in treated versus control limbs (p < .025). There were no differences in DDFT histopathologic scores between groups. The standing percutaneous DFTS adhesion induction model demonstrated that intrathecal enalapril reduced DFTS adhesion formation, lameness scores, and limb circumference over time. Intrathecal enalapril administration may reduce morbidity in horses with naturally occurring tendon injuries.

Is environmental risk assessment possible with the alternatives to acute fish toxicity test? Case study with pharmaceuticals

BackgroundAcute fish toxicity test (AFT) is one of the cornerstones of environmental risk assessment (ERA) of chemicals for the aquatic environment. Despite many efforts to find an alternative able to fully replace the test, there is still lasting pressure from stakeholders for AFT results. ResultsHere, we present the results of a case study with eight pharmaceuticals from various pharmaceutical groups with different levels of expected toxicity to fish. Selected compounds were tested in two validated alternative tests—fish embryo toxicity test with zebrafish (Danio rerio) (zFET) and in vitro RTgill-W1 assay according to their corresponding OECD guidelines TG 236 and TG 249, respectively. Data for AFT were collected from PubMed and ECOTOX knowledgebase databases, and acute toxicity to fish was further predicted in silico by the ECOSAR program. Predicted environmental risks (risk quotients, RQ, calculated using the exposure data from NORMAN) from both zFET and RTgill-W1 well correlated with the average RQs based on AFT LC50s. The strongest and most significant correlation was observed while comparing the AFT results with the median of combined alternative methods (zFET, RTgill-W1, ECOSAR).ConclusionsThis proposed approach combining experimental data with modeling could serve as a reliable tool for predictions of environmental risks promoting the 3R alternatives to acute fish toxicity testing.

The Reliability and Validity of the Rebound Countermovement Jump in National Collegiate Athletic Association Division I American Football Players.

Talpey, SW, Drake, M, Haintz, L, Belanger, A, O'Grady, MW, Young, WB, Mundy, PD, James L, Gabbett, TJ, Gardner, EC. The reliability and validity of the rebound countermovement jump in National Collegiate Athletic Association Division I American Football Players. J Strength Cond Res XX(X): 000-000, 2024-The purpose of this study was to investigate the reliability and validity of the rebound countermovement jump (R-CMJ), as an alternate test of leg muscle function to the CMJ and drop jumps (DJs). Because the first phase of the R-CMJ mimics that of the CMJ, it is hypothesized that variables obtained in phase I of the R-CMJ will be like those of a CMJ. Similarly, because the second phase of a R-CMJ mimics a DJ, it is believed that the variables obtained from phase II will be like those from a DJ. Collegiate male American football players (N = 46) aged 18-24 years completed CMJs and R-CMJs on a portable force plate. Tests were completed on 3 separate days to determine the repeatability of select variables and to establish their validity. In addition, a subgroup (N = 20) of players from the cohort completed DJs from both 30 cm (DJ30) and 45 cm (DJ45) to determine the concurrent validity of the variables obtained from the second phase of the R-CMJ. Most variables obtained from the CMJ and R-CMJ phase I were found to be reliable, except for time-to-take-off. The variables from the DJ30, DJ45, and R-CMJ phase II displayed moderate to good reliability; however, all coefficient of variations were >10%. Jump height achieved in the CMJ was significantly greater than that achieved in phase I of the R-CMJ. The reactive strength index was significantly different in the R-CMJ compared with the DJ from both 30 and 45 cm heights. Although the R-CMJ may be inviting for coaches to use owing to its ease and time efficiency, the results of this study highlight that it may not provide a valid representation of an athlete's reactive strength.

Analysis of Current Approaches to Monitoring the Content of Depressor Substances

INTRODUCTION. Raw materials of biological origin can contaminate medicines with depressor substances, such as histamine, acetylcholine, bradykinin, serotonin, and prostaglandins. In the Russian Federation, tests for the content of these impurities are conducted in anaesthetised healthy cats in accordance with the General Chapter Test for depressor substances of the State Pharmacopoeia of the Russian Federation. The global trend towards ending animal testing and the search for ways to eliminate animal tests for depressor substances motivate researchers to study international approaches to the determination of histamine-like substances.AIM. This study aimed to analyse Russian and international approaches to the control of biologicals for depressor substances and to consider the applicability of alternative instrument-based physicochemical methods for the determination of histamine-like impurities, with a view to reducing animal testing.DISCUSSION. The rationale for reducing the use of animals as test systems is set forth in Directive 2010/63/EU of the European Parliament and of the Council of 22 September 2010 on the protection of animals used for scientific purposes. In 2023, the European Pharmacopoeia Commission initiated the removal of in vivo tests for histamine-like substances from the European Pharmacopoeia. However, China and India use animal testing as the basic method for assessing the safety of medicines. In the Russian Federation, the control of depressor substances is regulated under General Chapter 1.2.4.0008.18 of the State Pharmacopoeia of the Russian Federation. Since the publication of General Chapter 42-0063-07 Test for histamine in edition XII in 2007, the State Pharmacopoeia of the Russian Federation has included a compendial text providing for a reduction in the number of cats used for testing. At present, Russian scientists are searching for targeted physicochemical and immunochemical methods for the determination of depressor substances. It has been demonstrated that a reasonable approach is to assess the need for testing a particular medicinal product in animals on a case-by-case basis. This approach will help minimise the number of in vivo tests and maintain an adequate quality of medicines at the same time.CONCLUSIONS. Despite the global trend towards ending animal testing, it is still necessary and required to control medicines for the content of depressor substances. Nevertheless, there is no global consensus on the need to use animals for this purpose. The absence of a separate general chapter/monograph, or references to it, will not change the fact that histamine-like substances are controlled worldwide. The decision to exclude in vivo methods from the European Pharmacopoeia necessitates the search for alternative test methods and the implementation of additional manufacturing controls to minimise the contamination risk for finished medicinal products. Studies have shown that high-performance liquid chromatography can determine the content of histamine in a limited amount of a medicinal product.

Impact Absorption Power of Polyolefin Fused Filament Fabrication 3D-Printed Sports Mouthguards: InVitro Study.

This study aims to evaluate and compare the impact absorption capacities of thermoformed ethylene vinyl acetate (EVA) mouthguards and 3D-printed polyolefin mouthguards used in sports dentistry applications. The objective is to determine whether 3D-printed polyolefin mouthguards offer superior impact toughness compared to traditional EVA mouthguards commonly used in sports settings. Six material samples were assessed: five pressure-formed EVA mouthguards (PolyShok, Buffalo Dental, Erkoflex, Proform, and Drufosoft) and one 3D-printed synthetic polymer (polyolefin). The materials were evaluated using a modified American Society for Testing and Materials (ASTM) D256 Test Method A for Izod pendulum impact resistance of plastics. Polyolefin samples were 3D-printed using fused filament fabrication (FFF) technology. Notably, the FFF process included samples printed with notches placed either parallel or perpendicular to the build direction. This orientation served as a study factor, allowing for comparison of material behavior under different printing conditions. Impact testing was conducted using an Izod impact tester to assess the materials' performance under controlled impact conditions. The study achieved a high power (1.0) in power analysis, indicating strong sensitivity to detect significant differences. Among molded materials, PolyShok showed significantly lower impact toughness compared to others (p = 0.06). The mean impact absorption of EVA materials was 5.4 ± 0.3 kJ/m2, significantly lower than polyolefin materials, which demonstrated 12.9 ± 0.7 kJ/m2 and superior performance (p = 0.0). Horizontal-notched polyolefin samples exhibited higher impact strength compared to vertical-notched samples (p = 0.009). 3D-printed polyolefin mouthguards exhibited significantly higher impact toughness than thermoformed EVA mouthguards. While EVA materials demonstrated structural robustness, their lower impact resistance and observed tearing in other test specimens suggest the need for alternative testing standards to better reflect real-world conditions. 3D-printed mouthguards fabricated with build orientations perpendicular to the direction of impact demonstrate significantly enhanced impact absorption. Further research into manufacturing methods and testing protocols is recommended to optimize mouthguard performance under impact scenarios.

The relationship of diabetes knowledge and self-care practices among patients with type 2 diabetes mellitus

Background: Diabetes Mellitus (DM) is a disease characterized by increased blood glucose levels and cannot be cured so it requires self-care. DM self-care is a program that must be carried out throughout the life of DM sufferers. DM self-care aims to optimize metabolic control, optimize quality of life, and prevent acute and chronic complications. Good knowledge and self-care consisting of good diet, physical exercise, medication, and education can control blood sugar levels and prevent complications of type 2 DM. Purpose: To determine the relationship between knowledge and self-care in patients with type 2 diabetes mellitus. Method: Descriptive correlational research design with a cross-sectional approach. The study population was patients with type 2 diabetes mellitus in the work area of ​​the Pasar Rebo District Health Center, East Jakarta, totaling 690 patients, carried out in November-December 2023. The sampling technique used the Accidental Sampling technique with the Slovin formula and a sample of 87 respondents was obtained. The research instrument used the Diabetes Knowledge Questionnaire (DKQ-24) for the knowledge questionnaire and the Summary of Diabetes Self Care Activity (SDSCA) for the self-care questionnaire. Data analysis used the univariate and bivariate Chi Square alternative tests. Results: Respondents with poor knowledge resulted in poor self-care of 30 respondents (53.6%), while respondents with good knowledge resulted in good self-care of 16 respondents (51.6%). The results of the Chi-Square test showed a significant p value of 0.001 (&lt;0.05). Conclusion: There is a significant relationship between knowledge and self-care in type 2 DM patients with a p value of 0.001.

Competition during verbal creative processes influences on ERS/ERD

ABSTRACT Humans are social creatures, and many tasks in our daily lives are solved together. The two main forms of social interaction in problem solving could be defined as competition and cooperation. In our study, we compared the ERS/ERD when performing a creative task (Alternative Uses Test, AUT) and a control task (“naming the objects from the presented category”) under competitive conditions in dyads (22 dyads, m-m, f-f, 18–23 years old) compared to the performance of tasks individually. The number of answers given by subjects under competitive conditions was significantly lower than during the execution of the tasks individually. The solving of the creative task in competition versus individual performance was accompanied by EEG synchronization (9–30 hz) clusters: 140–1220 ms and 900–1780 ms after stimulus presentation; 13.5–30 hz (1800–1980 ms), reflecting the creative thinking mode, and expected cognitive, emotional answers’ assessment. The control task under competitive conditions was accompanied by pronounced synchronization of low frequencies in the frontal areas (2–7 hz, 0–1980 ms), due to a greater working memory load; synchronization clusters in broadband (10–30 hz, 100–320 ms, 400–860 ms) and in the beta EEG band (17–30 hz, 1140–1980 ms). The competitive conditions significantly modulated the brain activity underlying creative and non-creative cognitive task performance, and resulted in greater induced EEG synchronization.

Sampling random graphs with specified degree sequences

The configuration model is a standard tool for uniformly generating random graphs with a specified degree sequence, and is often used as a null model to evaluate how much of an observed network’s structure can be explained by its degree structure alone. A Markov chain Monte Carlo (MCMC) algorithm, based on a degree-preserving double-edge swap, provides an asymptotic solution to sample from the configuration model. However, accurately and efficiently detecting when this Markov chain is sufficiently close to its stationary distribution remains an unsolved problem. Here, we provide a solution to sample from the configuration model using this standard MCMC algorithm. We develop an algorithm, based on the assortativity of the sampled graphs, for estimating the gap between effectively independent MCMC states, and a computationally efficient gap-estimation heuristic derived from analyzing a corpus of 509 empirical networks. We provide a convergence detection method based on the Dickey-Fuller Generalized Least Squares test, which we show is more accurate and efficient than three alternative Markov chain convergence tests. Supplementary materials for the proposed methods can be found here.

Consensus on drivers of maintenance treatment choice and patterns of care in advanced ovarian cancer

ObjectivesMaintenance therapies, including poly (ADP-ribose) polymerase (PARP) inhibitors and/or bevacizumab, have substantially improved the prognosis of patients with advanced ovarian cancer. Owing to the variability in treatment strategies across Europe,...

Pediatric Orally Disintegrating Tablets (ODTs) with Enhanced Palatability Based on Propranolol HCl Coground with Hydroxypropyl-β-Cyclodextrin

Background: Propranolol, largely prescribed as an antihypertensive and antiarrhythmic drug in pediatrics, is characterized by a bitter taste and an astringent aftertaste. Currently, the therapy requires crushing of tablets for adults and their dispersion in water many times a day, leading to loss of dosing accuracy, low palatability, and poor compliance for both patients and caregivers. Objectives: This work aimed to exploit cyclodextrin complexation by cogrinding to develop orally disintegrating tablets (ODTs) endowed with reliable dosing accuracy, good palatability and safety, ease of swallowability, and ultimately better compliance for both pediatric patients and caregivers. Results: Different formulation variables and process parameters were evaluated in preparing ODTs. The technological and morphological characterization and disintegration tests were performed according to official and alternative tests to select the ODT formulation based on the drug Hydroxypropyl-β-cyclodextrin (HPβCD) coground complex form containing Pearlitol® Flash as the diluent and 8% Explotab® as the superdisintegrant, which demonstrated the highest % drug dissolution in simulated saliva and acceptable in vitro palatability assessed by the electronic tongue, confirming the good taste-masking power of HPβCD towards propranolol. Conclusions: Such a new dosage form of propranolol could represent a valid alternative to the common extemporaneous preparations, overcoming the lack of solid formulations of propranolol intended for pediatric use.

Ambiguity of tone in annual reports and bank risk taking

AbstractIn this paper, we examine whether abnormal ambiguity of tone in banks' 10‐K filings is associated with banks' risk‐taking behaviour. We estimate abnormal ambiguous tone using residual of a tone model that disentangles the obfuscation component from the information component in the ambiguous tone. We find that banks that intentionally use more ambiguous tone in 10‐K filings exhibit higher risk taking subsequently, consistent with the argument that an intentional ambiguous tone adds to the opacity of financial statements, which potentially masks managerial risk‐taking activities and curbs market discipline from external stakeholders. Our results remain robust to alternative model specifications and sensitivity tests which address potential endogeneity concerns. Furthermore, banks with higher abnormal ambiguous tone are associated with more aggressive investment decisions. Together, our findings suggest that abnormal ambiguous tone in annual reports is informative of banks' risk‐taking activities.

Re-evaluating critical current density in solid-state batteries: Challenges and proposing an alternative

The critical current density (CCD) test protocols are widely adopted to examine the quality of solid electrolyte (SE), the stability of lithium (Li)/SE interfaces, and solid-solid interfacial kinetics in all solid-state lithium batteries (SSLBs). This work systematically investigated the impact of major experimental parameters on CCD values of Li symmetric cells using argyrodite Li6PS5Cl SE. Four categories of experimental parameters were investigated: (i) SE parameters, (ii) Li metal parameters, (iii) cell die parameters, and (iv) CCD testing protocols. Each parameter uniquely influences the density of the SE or the SE/Li interface, thereby affecting the resulting CCD values. Notably, CCD values are highly dependent on the cell operating histories, particularly the operating period (h) and the applied current density (mA/cm2). Consequently, without standardizing and unifying CCD testing protocols, obtaining reliable and repeatable CCD values remain challenging. To overcome this limitation, we propose the cumulative areal capacity (CAC) test protocol – the total capacity (mAh/cm2) delivered by a cell before its failure – as a more accurate measure of cell performance. CAC analysis of over 30 Li symmetric cells demonstrated statistical convergence in CAC values, highlighting its potential as a supplementary or even alternative testing protocol to conventional CCD methods.

Multifaceted Interventions to Enable Elimination of Clinician-ordered Blood Smear Reviews: A Rural Academic Medical Center Experience

Abstract Introduction/Objective Blood smears (BS) evaluate a variety of clinical disorders. At Geisinger, clinician ordered pathologist review of blood smears (CBS) have increased despite usage of highly sophisticated hematology analyzers and automatic blood smear (ABS) review criteria. We hypothesized that CBS impact pathologists’ and technologists’ workloads and increase costs and turn-around times, with rare improvement in value-based patient care. This study aimed to analyze CBS ordering practices and improve the utilization of BS review. Methods/Case Report In October 2020, a prospective CBS utilization study was completed by 8 clinical pathologists for 172 CBS. Subsequently, a clinical question was required for CBS orders from January 1, 2022, to January 1, 2023, to investigate clinician ordering practices based on campus, department, and indications. Findings were used to support new interventions including: clinician education; cancellation of repeat BS ordered within 24 hours, creation of alternative tests (schistocyte technologist smear review, clinician order for independent slide review), standardization of morphology reporting, ABS audits, and elimination of orderable CBS replaced by an electronic BS review request, that is reviewed by a clinical pathologist prior to determine if a BS is indicated. Results (if a Case Study enter NA) Part of the Conclusions section for Lab Practice abstract. Conclusion Prospective utilization audit of 172 CBS orders showed 17% changed clinical management. Audit of 3,693 CBS orders demonstrated common reasons for review included tick-borne illness, hemolysis, erythrocytosis, thrombocytosis, and iron deficiency anemia. Systematic implementation of multiple interventions enabled the elimination of CBS, with no known adverse effect on patient management to date. The streamlined BS review guidelines allowed for better-allocation of resources, appropriate patient turn-around times, empowerment of laboratory technologists and reduced hospital costs. We propose maximizing the incorporation of technologist skills to improve the utilization and efficiency of BS reviews and establishing standardized, evidence-based, and institutional- specific guidelines to determine BS reviews.

Uptake, Acceptability, and Results of SARS-CoV-2 Antigen Rapid Diagnostic Testing in Community Settings in Cameroon.

Mass gathering event restrictions were part of mitigation measures during the COVID-19 pandemic that were lifted as prevalence decreased and after vaccination rollout. We explored SARS-CoV-2 antigen rapid diagnostic test acceptability and positivity in community settings in Cameroon. In August-October 2022, community workers sensitized and referred individuals for COVID-19 testing to nearby testing points in Douala and Yaoundé. Participants consented to SARS-CoV-2 antigen rapid diagnostic testing, a survey, or both components. We describe the positivity rate, COVID-19-related history, and Likert-scale testing perceptions. Factors associated with testing acceptance were analyzed using logistic regression. Overall, 20.5% (2,449/11,945) of sensitized individuals visited testing points, and 1,864 (76.1%) were enrolled; 50.6% accepted the survey and testing (46.0% accepted survey only). Seven (0.7%) of 1,006 individuals tested positive. Most (71.8%; 1,292/1,800) considered community testing more accessible than hospital-based testing. Individuals accepting versus refusing testing differed in perceived COVID-19 risk (67%, 49%; P <0.001), belief in accurate test results (79%, 47%; P <0.001), and ability to test easily (96%, 55%; P <0.001). Males (adjusted odds ratio [aOR]: 1.26 [1.04-1.53]) and those over 50 years (aOR: 1.9 [1.4-2.7]), with symptoms (aOR: 1.80 [1.30-2.50]), and at least partial vaccination (aOR: 0.76 [0.58-0.99]) were significantly associated with test acceptance. Refusal reasons included lack of perceived need for testing (33.8%) and testing discomfort (26.3%). Although community-based testing was generally perceived as important, actual testing uptake was low. In future pandemics, community testing should be optimized by addressing misinformation, offering alternative testing modalities for greater comfort, creating demand, and tailoring approaches to maximize testing uptake.

Selectivity as discrimination: lessons from the case law on fiscal measures for identifying State aid

Abstract Article 107(1) of the Treaty on the Functioning of the European Union was originally designed to regulate the grant of subsidies by Member States, but it has been applied increasingly in recent years to regulate tax measures. Attempts to apply the State aid rules in this way have been met with controversy as they have been applied in unpredictable ways that have introduced significant changes to the existing tests in the case law for identifying State aid. These changes include a broader interpretation of the concept of selectivity, one of the criteria used to identify aid, which has been reoriented around the discrimination standard, whereby a measure is selective if it differentiates between categories of undertakings without being capable of justification by some legitimate public policy objective. Most controversially, this has facilitated the enforcement of the State aid rules against a wider range of State interventions. This article argues that the discrimination standard represents the most plausible and effective way of understanding selectivity among the alternative tests which have been proposed in the case law and academic literature. However, it goes on to identify a significant deficiency in the discrimination standard in the manner in which it is currently applied by the Union courts. The test inevitably requires certain value judgments to be made, but these are not made explicit by the existing case law. This article then proceeds to give further reflections on options for the refinement of the framework for assessing selectivity based on the discrimination standard and the existing logic of the case law and the objectives of the State aid control regime, which indicates how principled and coherent boundaries to the notion of State aid might be assessed in an individual case.

A Review of Biological Control One Decade After the Sorghum Aphid (Melanaphis sorghi) Outbreak.

Melanaphis sorghi is a pest that is native to Africa but is now distributed worldwide. In 2013, its destructive capacity was demonstrated when it devastated sorghum crops in the United States and Mexico, making it a new pest of economic importance in North America. At the time, the phytosanitary authorities of both countries recommended the use of pesticides to control the outbreak, and biological control products for the management of this pest were not known. In response to the outbreak of M. sorghi in North America, several field studies have been performed in the last decade on sorghum crops in the USA and Mexico. Works have focused on assessing resistant sorghum hybrids, pesticide use, and recruitment of associated aphid predators and entomopathogens for natural control of M. sorghi populations. The objective of this review is to compile the information that has been generated in the past decade about indigenous enemies affecting M. sorghi naturally in the field, as well as the search for biological control alternatives and evaluations of interactive effects of resistant sorghum hybrids, pesticides, and natural enemies. To date, different predators, parasitoids, fungi, and bacteria have been evaluated and in many cases found to affect M. sorghi populations in sorghum agroecosystems or laboratory bioassays, and the use of resistant sorghum varieties and pesticides did not have clear toxic effects on natural enemy populations. Many of the macroorganisms and microorganisms that have been evaluated as potential biological controls have shown potential as alternatives to synthetic pesticides for keeping M. sorghi population densities below economic damage thresholds and are compatible with integrated management of sorghum aphids. While most tests of these biological alternatives have shown that they have aphidicidal potential against sorghum aphids, it is crucial to take into account that their effectiveness in the field depends on a number of abiotic and biotic factors, including soil texture, temperature, humidity, and natural enemies.

Vocal Emotion Perception in Children Using Cochlear Implant.

The significance of emotional prosody in social communication is well-established, yet research on emotion perception among cochlear implant (CI) users is less extensive. This study aims to explore vocal emotion perception in children using CI and bimodal hearing devices and compare them with their normal hearing (NH) peers. The study involved children aged 4-10 years with unilateral CI and contralateral hearing aid (HA), matched with NH peers by gender and listening age. Children were selected using snowball sampling for the CI group and purposive sampling for the NH group. Vocal emotion perception was assessed for semantically neutral sentences in "happy," "sad," and "angry" emotions using a 3 alternate forced choice test. The NH group demonstrated significantly superior emotion perception (P=.002) compared to the CI group. Both groups accurately identified the "happy" emotion. However, the NH group had higher scores for the "angry" emotion compared to the "sad" emotion, while the CI group showed better scores for "sad" than "angry" emotion. Bimodal hearing devices improved recognition of "sad"and "angry" emotions, with a decrease in confusion percentages. The unbiased hit (Hu) value provided more substantial insight than the hit score. Bimodal hearing devices enhance the perception of "sad" and "angry" vocal emotions compared to using a CI alone, likely due to the HA providing the temporal fine structure cues, thereby better representing fundamental frequency variations. Children with unilateral CI benefit significantly in the perception of emotions by using a HA in the contralateral ear, aiding in better socio-emotional development.

Stem extract from Costus afer (Bush cane) prevents ethanol-induced neuronal degeneration in mice via an antioxidant-inflammatory pathway

BackgroundExcessive alcohol consumption is associated with memory impairment and can lead to Alzheimer's disease-like dementia, contributing to the rising prevalence of dementia worldwide. Costus afer (Bush cane) is a medicinal plant widely used in traditional African medicine for its anti-inflammatory, antioxidant, and neuroprotective properties. It is commonly employed to treat various ailments, including inflammatory diseases, respiratory conditions, and gastrointestinal disorders. PurposeGiven its traditional use in the treatment of kyphosis (hunchback) which is implicated in cerebral palsy and degenerative disease of the spine, we explored its potential neuroprotective effects against ethanol-induced neuronal degeneration, in this research, we examined the potential protective impacts on memory and neurology afforded by the aqueous stem extract derived from C. afer in mice subjected to ethanol exposure. MethodsForty-five male Swiss mice weighing 22–35 g were divided into five groups (n = 9): control (treated with distilled water orally), memory-impaired (treated with ethanol at 5 g/kg orally), and three groups treated with ethanol followed by administration of C. afer at doses of 100 mg/kg, 200 mg/kg, and 500 mg/kg respectively. All animals were provided unrestricted access to both food and water throughout the duration of the study. Memory impairment was assessed after 28 days using the T-maze spontaneous alternation test. Subsequently, spectrophotometry, ELISA, and histomorphometry were employed to evaluate indicators of neuronal inflammation, oxidative stress linked to inflammation, and degenerative changes were assessed in the hippocampus, prefrontal cortex, and cerebellum. ResultsEthanol administration led to a notable (p < 0.01) decline in neurobehavioral function, as evidenced by reduced spontaneous alternation behavior, which was mitigated by C. afer treatment, leading to increased percentage alternation. Furthermore, ethanol administration altered endogenous antioxidant levels and pro-inflammatory mediators, resulting in elevated lipid peroxidation, nitrite, tumor necrosis factor-alpha, interleukin-6, and decreased superoxide dismutase activity, promoting neuronal degeneration in the mice brains. However, treatment with C. afer at doses of 100 mg/kg, 200 mg/kg, and 500 mg/kg substantially (p < 0.05) attenuated oxidoinflammatory stress by reducing levels of MDA, NO-2, TNF-α, IL-6, while upregulating SOD activity, thereby preserving neuronal integrity in the hippocampus, prefrontal cortex, and cerebellum. ConclusionThese findings suggest that C. afer mitigates the progression of memory impairment induced by ethanol through mechanisms involving the suppression of oxidoinflammatory stress facilitators and inhibition of cortico-hippocampal and cerebellar neuronal degeneration in murine subjects.

Interaction Between Age Pension Means Testing and Innovative Income Streams in Australia*

We investigate the interaction between Age Pension means testing and innovative income streams in Australia, considering the use of an investment‐linked annuity that allows a choice of drawdown level. We find that current means testing arrangements incentivise a very specific usage of investment‐linked annuities that is not materially impacted by individual characteristics or fees, due to incoherencies across the income test and assets test. We investigate alternative means test structures, including a structure that includes only an income test and incorporates capital consumption by adjusting taper rates with age according to an annuitisation schedule. This structure removes the distortions impacting annuity usage, generates the most stable Age Pension over lifetime, encourages drawdown of assets over retirement and more accurately reflects the means of retirees to generate an income at each age.