Tertiary Butylhydroquinone Research Articles

Comparative Study of an Antioxidant Compound and Ethoxyquin on Feed Oxidative Stability and on Performance, Antioxidant Capacity, and Intestinal Health in Starter Broiler Chickens.

Concerns over the safety of ethoxyquin (EQ) highlight the need for safer, more effective feed antioxidants. This study investigated a healthier antioxidant compound (AC) as a potential alternative to EQ in broilers. A total of 351 one-day-old Arbor Acres Plus male broilers were randomly assigned to three treatments for 21 days: control (CON), EQ group (200 g/ton EQ at 60% purity), and AC group (200 g/ton AC containing 18% butylated hydroxytoluene, 3% citric acid, and 1% tertiary butylhydroquinone). AC supplementation reduced the acid value, peroxide value, and malondialdehyde content in stored feed, decreased feed intake and the feed conversion ratio without affecting body weight gain, and enhanced antioxidant capacity (liver total antioxidant capacity and superoxide dismutase; intestinal catalase and glutathione peroxidase 7). It improved intestinal morphology and decreased barrier permeability (lower diamine oxidase and D-lactate), potentially by promoting ZO-1, Occludin, and Mucin2 expression. The AC also upregulated NF-κB p50 and its inhibitor (NF-κB p105), enhancing immune regulation. Additionally, the AC tended to increase beneficial gut microbiota, including Lactobacillus, and reduced Bacteroides, Corprococcus, and Anaeroplasma. Compared to EQ, the AC further enhanced feed oxidative stability, the feed conversion ratio, intestinal morphology and barrier functions, and inflammatory status, suggesting its potential as a superior alternative to EQ for broiler diets.

Covalent organic framework composite hydrogel sorbent beads for vortex-assisted dispersive miniaturized solid phase extraction of parabens and synthetic phenolic antioxidants in foodstuffs

A covalent organic framework composite hydrogel sorbent beads was fabricated for the vortex-assisted dispersive miniaturized-solid phase extraction and determination of parabens and synthetic phenolic antioxidants from food matrices. The covalent organic framework was entrapped into alginate hydrogel beads. This composite sorbent effectively adsorbs analytes through hydrophobic, π-π and hydrogen bonding interactions. Under optimal extraction conditions, the developed method demonstrated good linearity from 1.00 to 100 µg kg−1 for methyl paraben and 2.00 to 100 µg kg−1 for tertiary butylhydroquinone, propyl paraben, butyl paraben and butylated hydroxyanisole. Limits of detection ranged from 0.25 to 0.50 µg kg−1. The developed sorbent was applied to the extraction of parabens and synthetic phenolic antioxidants from tomato paste, mayonnaise, infant milk powder and coffee creamer samples. The analytes were separated and quantified by high-performance liquid chromatography, achieving recoveries ranging from 89 to 98 % with RSDs lower than 7 %. The vortex-assisted extraction approach was efficient, and the fabricated sorbent could be reused for up to eight cycles. The greenness of the methodology was also evaluated.

Fabrication of copper tungstate nanoparticles on screen printed carbon electrode: A disposable strip for the electrochemical detection of the food additive tertiary Butylhydroquinone

Food additives enhance sensory pleasure and improve marketability in food product formulations, yet their potential health risks are highly consequential. Tertiary butylhydroquinone (TBHQ), in this regard, has a controversial reputation owing to its neurotoxic effects, which include convulsions and other chronic issues. This situation underscores the need for an advanced electrochemical sensing platform. The current study advocates the utilization of copper tungstate nanoparticles to enhance the sensitivity, selectivity, and efficiency in TBHQ detection, thus addressing the challenges posed by the excessive use of additives and safeguarding the integrity of the food supply chain. Hydrothermally synthesized CuWO4 nanoparticles exhibited superior physicochemical and morphological characteristics, bringing about wide linear response ranges (0.01 to 789 μM), high selectivity, excellent anti-interference capabilities, and a low detection limit of 0.9 nM (S/N = 3). The CuWO4 modified screen printed carbon electrode (SPCE) presents promising recovery ranges in food samples, facilitating real-time monitoring and streamlining the quality assessment of food additives.

Protective Effect of Tertiary Butylhydroquinone against Obesity-induced Skeletal Muscle Pathology in Post-weaning High Fat Diet Fed Rats.

Obesity deleteriously affects skeletal muscle functionality starting from infancy to adulthood, leading to dysfunctional skeletal muscle. This study, therefore, evaluated the protective action of tert-butylhydroquinone (tBHQ) against obesity-induced skeletal muscle pathology in high-fat diet (HFD) fed rats. Twenty post-weaning male albino rats were randomized into four groups of five rats each as: Group 1 (control), Group 2 (HFD), Group 3 (orlistat) and Group 4 (tBHQ). Group one received rat pellets for 12 weeks, while groups 2 to 4 received HFD for 12 weeks. At the end of week 8, obesity was confirmed with Lee Obesity Index and body mass index values of ≥ 303 and ≥ 0.68 gcm2, respectively. Group 3 was given oral administration of orlistat (10 mg/kg, once daily), while group 4 was given oral administration of tBHQ (25 mg/kg, once daily). Administration of orlistat and tBHQ commenced from week 9 to the end of the experiment. Chronic exposure of post-weaning rats to HFD led to their development of the metabolic syndrome phenotypes in adulthood, characterized by obesity, hyperglycemia, dyslipidaemia, hyperinsulinaemia, insulin resistance as well as induction of oxidative stress and alteration of skeletal muscle markers, which were mitigated following supplementation with orlistat and tBHQ. The study showed the anti-obesity potentials of tBHQ and its protective action against HFD obesity-induced skeletal muscular pathology.

Nickel Tungstate Nanoparticle-Modified Screen-Printed Carbon Electrode: The Disposal Strip for the Electrochemical Detection of Tertiary Butylhydroquinone

Nickel Tungstate Nanoparticle-Modified Screen-Printed Carbon Electrode: The Disposal Strip for the Electrochemical Detection of Tertiary Butylhydroquinone

The effect and mechanism of combined exposure of MC-LR and NaNO2 on liver lipid metabolism

Microcystin-LR (MC-LR) and sodium nitrite (NaNO2) co-exist in the environment and are hepatotoxic. The liver has the function of lipid metabolism, but the impacts and mechanisms of MC-LR and NaNO2 on liver lipid metabolism are unclear. Therefore, we established a chronic exposure model of Balb/c mice and used LO2 cells for in vitro verification to investigate the effects and mechanisms of liver lipid metabolism caused by MC-LR and NaNO2. The results showed that after 6 months of exposure to MC-LR and NaNO2, the lipid droplets content was increased, and the activities of alanine aminotransferase (ALT) and aspartate aminotransferase (AST) were raised in the liver (P < 0.05). Moreover, MC-LR and NaNO2 synergistically induced hepatic oxidative stress by decreasing total superoxide dismutase (T-SOD) activity and glutathione (GSH) levels and increasing malondialdehyde (MDA) content levels. In addition, the levels of Nrf2, HO-1, NQO1 and P-AMPK was decreased and Keap1 was increased in the Nrf2/HO-1 pathway. The key factors of lipid metabolism, SREBP-1c, FASN and ACC, were up-regulated in the liver. More importantly, there was a combined effect on lipid deposition of MC-LR and NaNO2 co-exposure. In vitro experiments, MC-LR and NaNO2-induced lipid deposition and changes in lipid metabolism-related changes were mitigated after activation of the Nrf2/HO-1 signaling pathway by the Nrf2 activator tertiary butylhydroquinone (TBHQ). Additionally, TBHQ alleviated the rise of reactive oxygen species (ROS) in LO2 cells induced by MC-LR and NaNO2. Overall, our findings indicated that MC-LR and NaNO2 can cause abnormal liver lipid metabolism, and the combined effects were observed after MC-LR and NaNO2 co-exposure. The Nrf2/HO-1 signal pathway may be a potential target for prevention and control of liver toxicity caused by MC-LR and NaNO2.

Bio-based lubricant with additives: tribological performances in hydrodynamic journal bearing

PurposeThe purpose of this paper is to investigate the performance of bio-based lubricants (BBL), namely, palm mid-olein (PMO) enriched with an antioxidant agent, tertiary-butylhydroquinone (TBHQ) and a viscosity improver, ethylene-vinyl acetate (EVA), in journal bearing (JB) applications.Design/methodology/approachSamples of the BBL were prepared by blending it with TBHQ and EVA at various blending ratios. The oxidative stability (OS) and viscosity of the BBL samples were examined using differential scanning calorimetry and a viscometer, respectively. Meanwhile, their performance in JB applications was evaluated through the use of a JB test rig with a 0.5 length-to-diameter ratio at various operating conditions.FindingsIt was found that the combination of PMO + TBHQ + EVA demonstrated a superior oil film pressure and load-carrying capacity, resulting in a reduced friction coefficient and a smaller attitude angle compared to the use of only PMO or VG68. However, it was observed that the addition of TBHQ and EVA to the PMO did not have a significant impact on the minimum oil film thickness.Practical implicationsThe results would be quite useful for researchers generally and designers of bearings in particular.Originality/valueThis study used PMO as the base stock, and its compatibility with TBHQ and EVA was investigated in terms of its OS and viscosity. The performance of this treated BBL was evaluated in a hydrodynamic JB.Peer reviewThe peer review history for this article is available at: https://publons.com/publon/10.1108/ILT-11-2023-0363/

Assessing the influence of Secoisolariciresinol diglucoside multi‐antioxidants on flaxseed oil's oxidative stability

AbstractTo increase the oxidative stability of flaxseed oil (FSO), combinations of two synergists (vitamin C, citric acid) and five distinct antioxidants (SDG (Secoisolariciresinol diglucoside), tea polyphenol (TP), resveratrol (RE), caffeic acid (CA), and vitamin E (VE)) were assessed. Based on the accelerated oxidation reaction at 65°C, the antioxidant effect was assessed for changes in the shelf‐life, fatty acid composition, peroxide value (POV), volatile and sterol contents of FSO. The SDG multi‐antioxidant combination, which included 0.01% SDG, 0.01% TP, and 0.02% vitamin C, demonstrated the strongest antioxidative qualities, according to the data; the POV of FSO dropped by 57.5% as compared to the blank group. Additionally, the SDG multi‐antioxidant combination outperformed 0.02% TBHQ (tertiary butylhydroquinone), increased the shelf‐life of FSO from 295 to 761 days, and postponed the deterioration of sterols and unsaturated fatty acids. Furthermore, the production of ketones and the degradation of esters and acids were diminished. In summary, the combination of SDG‐TP and vitamin C as antioxidants can be an effective natural alternative to artificial antioxidants to extend the shelf‐life of FSO.Practical Applications: Flaxseed oil utilizing multi‐antioxidant combinations has a wide range of practical applications. Multi‐antioxidant combination of flaxseed oil helps prevent cell damage and slows down the aging process. Antioxidant‐rich oils have numerous health benefits such as reducing inflammation, promoting heart health and improving digestive health. Multi‐antioxidant combination flaxseed oil is versatile and a valuable natural product that can be used in all aspects of daily life to improve overall health, It can provide a theoretical reference for the selection of antioxidants to improve the oxidative stability of flaxseed oil, provide a theoretical basis for the replacement of artificial antioxidants, and also provide certain technical support for the industrial application of flaxseed oil.

Alkaline sphingomyelinase deficiency impairs intestinal mucosal barrier integrity and reduces antioxidant capacity in dextran sulfate sodium-induced colitis.

Ulcerative colitis is a chronic inflammatory disease of the colon with an unknown etiology. Alkaline sphingomyelinase (alk-SMase) is specifically expressed by intestinal epithelial cells, and has been reported to play an anti-inflammatory role. However, the underlying mechanism is still unclear. To explore the mechanism of alk-SMase anti-inflammatory effects on intestinal barrier function and oxidative stress in dextran sulfate sodium (DSS)-induced colitis. Mice were administered 3% DSS drinking water, and disease activity index was determined to evaluate the status of colitis. Intestinal permeability was evaluated by gavage administration of fluorescein isothiocyanate dextran, and bacterial translocation was evaluated by measuring serum lipopolysaccharide. Intestinal epithelial cell ultrastructure was observed by electron microscopy. Western blotting and quantitative real-time reverse transcription-polymerase chain reaction were used to detect the expression of intestinal barrier proteins and mRNA, respectively. Serum oxidant and antioxidant marker levels were analyzed using commercial kits to assess oxidative stress levels. Compared to wild-type (WT) mice, inflammation and intestinal permeability in alk-SMase knockout (KO) mice were more severe beginning 4 d after DSS induction. The mRNA and protein levels of intestinal barrier proteins, including zonula occludens-1, occludin, claudin-3, claudin-5, claudin-8, mucin 2, and secretory immunoglobulin A, were significantly reduced on 4 d after DSS treatment. Ultrastructural observations revealed progressive damage to the tight junctions of intestinal epithelial cells. Furthermore, by day 4, mitochondria appeared swollen and degenerated. Additionally, compared to WT mice, serum malondialdehyde levels in KO mice were higher, and the antioxidant capacity was significantly lower. The expression of the transcription factor nuclear factor erythroid 2-related factor 2 (Nrf2) in the colonic mucosal tissue of KO mice was significantly decreased after DSS treatment. mRNA levels of Nrf2-regulated downstream antioxidant enzymes were also decreased. Finally, colitis in KO mice could be effectively relieved by the injection of tertiary butylhydroquinone, which is an Nrf2 activator. Alk-SMase regulates the stability of the intestinal mucosal barrier and enhances antioxidant activity through the Nrf2 signaling pathway.

Improving physicochemical and tribological characteristics of bio-lubricants using carbon-based hybrid nanomaterials modified by tertiary-butyl-hydroquinone

Vegetable oils are being increasingly studied in tribology due to their environmental benefits. This study investigates the physicochemical and tribological properties of carbon black/silver (CB/Ag) hybrid nanomaterials modified by tertiary-butylhydroquinone (TBHQ) as lubricant additives. The composition of the tribolayer formed were characterized using an FE-SEM, 3D surface profilometer, and XPS analysis. The results reveal a substantial decrease in the coefficient of friction (COF) value for the hybrid nanolubricant, with an approximate 31% reduction compared to the baseline oil. Finally, the characterization of worn surfaces strongly supports the noteworthy improvement in friction performance, primarily ascribed to the synergistic effects of CB, Ag nanoparticles, and TBHQ antioxidant surfactants. This study affirms that TBHQ-modified CB/Ag hybrid nanoparticles serve as eco-friendly lubricant additives.

Preparation and structural characterization of epoxidized soybean oils-based pressure sensitive adhesive grafted with tea polyphenol palmitate

Vegetable oils-based pressure sensitive adhesives (PSAs) are green and sustainable but face unsatisfactory adhesion strengths and are prone to aging during storage and application due to the existence of residual double bonds and massive ester bonds. Nine common antioxidants (tea polyphenol palmitate (TPP), caffeic acid, ferulic acid, gallic acid, butylated hydroxytoluene, tertiary butylhydroquinone, butylated hydroxyanisole, propyl gallate, and tea polyphenols) were grafted into epoxidized soybean oils-PSA (ESO-PSA) system to enhance antiaging properties and adhesion strengths. Results showed ESO-PSAs grafted with caffeic acid, tertiary butylhydroquinone, butylated hydroxyanisole, propyl gallate, tea polyphenols, or TPP didn't occur failure with TPP having best performance. The optimal conditions were ESO reacted with 0.9 % TPP, 70 % rosin ester, and 7.0 % phosphoric acid at 50 °C for 5 min, under which peel strength and loop tack increased to 2.460 N/cm and 1.66 N, respectively, but peel strength residue reduced to 138.09 %, compared with control (0.407 N/cm, 0.43 N, and 1669.99 %). Differential scanning calorimetry and thermogravimetric results showed TPP grafting increased the glass transition temperature of ESO-PSA slightly but improved its thermal stability significantly. Fourier transform infrared spectroscopy and 1H nuclear magnetic resonance results showed TPP, phosphoric acid, and rosin ester all partially participated in the covalently crosslinking polymerization of ESO-PSAs and the rest existed in the network structures in the free form.

Effects of Complex Antioxidants Added to Chicken Diet on Growth Performance, Serum Biochemical Indices, Meat Quality, and Antioxidant Capacity

This study aimed to evaluate the effects of diets supplemented with various levels of complex antioxidants (CA) containing tertiary butylhydroquinone (TBHQ) and tea polyphenols (TP) on growth performance, meat quality of breast and leg muscles, serum biochemistry, and antioxidant capacity of serum, liver, breast meat, jejunum, and ileum in broilers. A total of 600 one-day-old Arbor Acres male broilers with similar body weights were randomly divided into three groups (10 replicates/group, 20 broilers/replicate). Birds in the three experimental groups were fed a basal diet with CA at 0, 300, and 500 mg/kg. The results showed that supplementing with 300 mg/kg CA significantly increased (p &lt; 0.05) 42 d BW and 22–42 d ADG, and markedly decreased (p &lt; 0.05) 22–42 d F: G ratio in comparison to the control group. Birds fed a diet with 300 mg/kg CA had a higher (p &lt; 0.05) pH of chicken meat at 24 h and 48 h post mortem and lower (p &lt; 0.05) yellowness values (b*) of chicken meat at 45 min and 24 h post mortem, along with a lower (p &lt; 0.05) cooking loss. Supplementing with 300 mg/kg CA significantly increased (p &lt; 0.05) serum and liver T-SOD activity, serum T-AOC level, as well as jejunual GST activity, and significantly decreased (p &lt; 0.05) liver MDA content when compared with the control group. These results indicate that diet supplementation with 300 mg/kg CA containing TBHQ and TP could improve growth performance and meat quality by increasing the antioxidant capacity of broilers.

MicroRNA-204-5p Ameliorates Renal Injury via Regulating Keap1/Nrf2 Pathway in Diabetic Kidney Disease.

Diabetic kidney disease (DKD) is characterized by renal fibrosis, and the pathogenesis of renal fibrosis is still not definitely confirmed. MiR-204-5p plays an important role in the regulation of fibrosis, autophagy and oxidative stress. In this study, we aimed to investigate the role of miR-204-5p on renal damage in diabetic kidneys and the underlying mechanisms involved. In vivo, AAV-Ksp-miR-204-5p mimics were injected into mice via tail vein. In vitro, high glucose-induced HK-2 cells were treated with miR-204-5p inhibitor, miR-204-5p mimics, ATG5 siRNA, tertiary butyl hydroquinone (TBHQ), ML385, or 3-Methyladenine (3-MA). FISH and qRT-PCR were used to detect miR-204-5p expression. The expressions of protein and mRNA were detected by Western blotting, immunofluorescence, immunohistochemistry and qRT-PCR. The concentration of fibronectin in HK-2 cells culture medium was detected by ELISA. The expression of miR-204-5p in diabetic kidneys was significantly inhibited than that in control group. Delivering miR-204-5p mimics increased miR-204-5p expression, improved renal function, inhibited renal fibrosis and oxidative stress, and restored autophagy in db/db mice. In vitro, the expression of miR-204-5p was inhibited by HG treatment in HK-2 cells. MiR-204-5p mimics effectively increased miR-204-5p expression and reduced fibronectin and collagen I expression, restored autophagy dysfunction, and increased Nrf2 expression, whereas these alterations were abrogated by Nrf2 inhibitor ML385, autophagy inhibitor 3-methyladenine (3-MA, 5 mM) treatment or ATG5 siRNA transfection in HG-induced HK-2 cells. In addition, miR-204-5p inhibitor significantly inhibited miR-204-5p expression and aggravated HG-induced fibronectin and collagen I expression, autophagy dysfunction, and decreased Nrf2 expression, while these alterations were abolished by Nrf2 activator TBHQ. Furthermore, the binding of miR-204-5p with Keap1 was confirmed by luciferase reporter assay and miR-204-5p negatively regulated Keap1 expression, resulting in the activation of Nrf2 pathway. MicroRNA-204-5p protects against the progression of diabetic renal fibrosis by restoring autophagy via regulating Keap1/Nrf2 pathway.

Binary Effect of Tertiary Butylhydroquinone and Butylated Hydroxytoluene Additives with The Addition of Glycerol Monostearate to Improve Oxidative Stability of Palm Oil-Based Biodiesel

Biodiesel is a renewable and environmentally friendly alternative to conventional diesel fuel. However, like any fuel, biodiesel is subject to oxidation, which can negatively impact its quality and performance when kept for long-term. The addition of binary antioxidants such as TBHQ:BHT had been proven to improve oxidation stability of biodiesel. Combining surfactant such as GMS into single antioxidant had been proven to solve its insolubility issue. However, the implementation of mixing binary antioxidants and surfactant has not been done yet. Therefore, this research analyzed the effect of single antioxidant, binary antioxidants, and binary antioxidants with GMS (100 ppm) addition into biodiesel and biodiesel blend B35. The effect was observed within 8 weeks storage period. The result showed that B35 did not have any significant impact. While in pure biodiesel samples, B100-bi and B100-bi+GMS had a slight difference in the results of oxidative parameters. B100-bi showed the best result in induction period and kinematic viscosity. Rancimat test showed 170 hours for B100-bi and 168 hours for B100-bi+GMS. While B100-bi+GMS indicated as the best additives in term of acid number, iodine value, and dispersion test. Hence, the addition of surfactant into binary antioxidants showed similar performance with B100-bi but with slightly better solubility.

THE IMPACT OF TEMPERATURE AND ANTIOXIDANTS ON OXIDATION AND THE FORMATION OF TRANS FATTY ACIDS IN SEVERAL PALM OIL DERIVATIVES

The quality of palm oil is strongly influenced by temperature. The oxidation of palm oil can be caused by high temperatures. The higher the storage temperature, the faster oxidation occurs. In this research, the temperature significantly affects the quality of several palm oil derivative products such as Palm Olein, Palm Stearin, Palm Kernel Oil, and Palm Kernel Stearin. Antioxidants are very effective in resisting the increase in the oxidation reaction of some palm oil commodities. With the addition of tertiary butylhydroquinone (TBHQ) or butylated hydroxytoluene (BHT), the increase in peroxide value, p-anisidine values, free fatty acid, color tends to be slower than without the addition of antioxidants. For PV, BHT was the most effective antioxidant for RPKOL at a 29.75% increase, RBDOL at 9.91%, and RBDST at 5.26% but for RPKS TBHQ was the most effective increase at 19.83%. For anisidine value, TBHQ+BHT was most effective for RPKOL with a 61.62% increase, TBHQ for RPKS with 76.76%, BHT for RBDOL and RBDST with 15.37% and 14.46%. In the FFA test, BHT was effective for RPKOL and RPKS with a percentage increase of 6.48% and 6.67%, but TBHQ was effective for RBDOL and RBDST to controlled FFA with an increase of 8.41% and 3.38%. In color testing, TBHQ proved to be the effective antioxidant for RPKOL and RBDOL, resulting in percentage increases of 8.71% and 2.13%, respectively. For RPKS, the combination of TBHQ and BHT was employed, resulting in no percentage increase (0%). Additionally, BHT at 3.85% was used for RBDST. At a temperature of 130°C, an oxidation process was observed, indicating the formation of an aldehyde compound. This was evidenced by the FT-IR spectrum, showing peaks at wave numbers 1760 cm⁻¹ (C=O of aldehyde) and 2900 cm⁻¹ (CH on aldehyde). In this research, we can also see the formation of trans fatty acids C18:1n9t in GC-FID's peak area

INVESTIGATING THE COMPARATIVE ANTIOXIDANT PARTITIONING IN FRIED OILS AND FRIED POTATOES: A SCIENTIFIC EVALUATION

Frying is a complex process that enhances the attractiveness and flavor of the final product. In this specific investigation, potato slices were subjected to frying using sun flower oil, and an antioxidant was introduced at a concentration of 200 ppm in accordance with FDA guidelines. Both the potato slices and the frying oil were analyzed to determine the levels of antioxidants after several frying cycles. The primary objective of this research was to evaluate the distribution of butylated hydroxyanisole (BHA), tertiary butyl hydroquinone (TBHQ), and propyl gallate (PG) in the fried potato slices and the frying oils. To conduct the experiments, batches of 50 grams of sliced potatoes were fried at 180ºC for 3-5 minutes per batch. The results showed that TBHQ demonstrated a superior ability to retain antioxidants compared to the other two types. TBHQ experienced significant degradation at frying temperatures, which likely contributes to the stability of the frying oil. The by-products generated during this degradation process impede the radical chain elongation process. As a result, the effectiveness of the antioxidants followed this order: TBHQ &gt; BHA &gt; PG.

Effects of tea polyphenols and tertiary butylhydroquinone on quality of palm oils and losses of endogenous vitamin E during batch frying and oxidative stability of fried instant noodles

The effects of tea polyphenols (TP) on the quality of palm oils (PO) and losses of endogenous vitamin E during batch frying of instant noodles as well as oxidative stability of fried instant noodles were investigated. PO without antioxidant addition was negative control and with tertiary butylhydroquinone (TBHQ) addition positive control. TP and TBHQ addition inhibited the increase of peroxide, p-anisidine, and total oxidation values of PO and reduced tocopherol and tocotrienol losses with 200 mg/kg of TP having the best performance, but didn’t affect acid value and triglyceride composition. 200 mg/kg of TP and 100 mg/kg of TBHQ inhibited unsaturated fatty acid losses. During frying, TBHQ was mainly volatilized but TP transformed. TP more effectively reduced tocopherol and tocotrienol losses than TBHQ, reducing PO deterioration. The extended lifecycles of PO and shelf life of fried instant noodles are attributed to nonvolatility of TP and antioxidative properties of its transformation products.

Hydroxytyrosyl oleate is a promising safe additive to inhibit the oxidation of olive oil

The antioxidants used in food oils that are prone to oxidation have attracted broad attention from the food industry. Hydroxytyrosol is a potent antioxidant, but due to its low solubility in oil, hydroxytyrosyl oleate was prepared, and its antioxidant capacity in the bulk oil was comprehensively evaluated. Hydroxytyrosyl oleate exhibited moderate free radicals scavenging, Fe3+ reducing, and oxygen adsorption abilities. Moreover, during accelerated oxidation, hydroxytyrosyl oleate delayed olive oil's peroxide value generation, acid value increase, and hydroperoxides production better than hydroxytyrosol (HT), tertiary butylhydroquinone (TBHQ), butylated hydroxytoluene (BHT), and Vitamin E (VE). The induction period of olive oil containing hydroxytyrosyl oleate was 77.12 h, which was 1.6, 8.7, 3.0, and 4.0 times that of HT, VE, BHT and TBHQ, respectively. Furthermore, hydroxytyrosyl oleate significantly prevented fatty acid oxidation and undesirable odors production in olive oil. These results indicate that hydroxytyrosyl oleate is a safe antioxidant that could be widely used in foods rich in polyunsaturated fatty acids, thus reducing lipid oxidation and food spoilage.

Effect of Added Acrylate Demulsifier for Increasing Antioxidants Functional in Diesel Fuel Oil

Biodiesel is produced by an esterification process with hygroscopic chemicals, otherwise the biodiesel is very easy to produce water, and the hydrocarbon compounds were easily oxidized, causing corrosion in the stockpiling process such as in storage tanks and distribution pipes. Antioxidants have been shown to reduce the formation of water in the biodiesel stockpiling process. While the demulsifier plays a role in helping to separate water and oil. In this study, the addition of an acrylate-based demulsifier in the accumulation of biodiesel-containing antioxidants was carried out. The antioxidant used in the form of tertiary butylhydroquinone with a concentration of 0.5 M has been able to play an active role in inhibiting the formation of water in the biodiesel stockpiling process. However, the stability of the water and oil emulsion has not been achieved properly, therefore the addition of an acrylate-based demulsifier with a volume of 2 mL, 5 mL, and 10 mL into 100 mL biodiesel can separate oil and water well. The biodiesel that has been added with a demulsifier is evaluated at storage times of 24 hours, 48 hours, and 72 hours. The result is that the storage for 72 hours is more effective in separating water and oil than storage for 24 hours and 48 hours. To evaluate the water content in the biodiesel stockpiling process out using 831 KF Coulometric and Fourier-Transform Infrared Spectroscopy analysis.

Efek Penambahan Antioksidan dan Demulsifier Secara Polimerisasi Pada Proses Penimbunan Diesel Fuel

B30 is an environmentally friendly fuel used for diesel engines. B30 is very easily contaminated with water sourced from the production process and raw materials from the B30. This water content causes corrosion and has an impact on landfilling. A demulsifier is a way to break down the B30 and water emulsion. In this study, the demulsifier used was acrylate-based and mixed with polar and non-polar solvents. The addition of a demulsifier with a volume of 2 mL, 5 mL, and 10 mL in the stockpiling process with variations of 2 hours, 4 hours, and 6 hours obtained an effect on the volume of water, where the volume of water increases with longer storage time. But the effectiveness of the demulsifier can be evaluated by potentiometrically measuring the water content and FTIR. The demulsifier that was made was also added with an antioxidant in the form of tertiary butylhydroquinone (TBHQ) with a concentration of 0.5 M and obtained more separate water layers than without the addition of TBHQ. To obtain the water content value after adding TBHQ, the coulometric and Fourier Transform Infra Red methods were used, which yielded higher results when compared to the absence of TBHQ. So that antioxidants and dimulsifiers have a positive effect on the process of storing diesel fuel.