AbstractSpectrally mixed pixels are the rule, not the exception, in decameter terrestrial imaging. By definition, the reflectance spectrum of a mixed pixel is a function of more than one generative process. Physically based surface biology and geology retrievals must therefore isolate the component of interest from a myriad of unrelated processes, each occurring with unknown presence and abundance across the hundreds of square meters sampled by each pixel. Foliar traits, for example, must be isolated from canopy structure and substrate composition. In many cases, these unrelated processes can dominate overall variance of spatially integrated reflectance. We propose a new approach to isolate low‐variance spectral signatures in mixed pixels. The aggregate effects of (high‐variance) spatial mixing processes within each pixel are modeled by treating the observed reflectance as a linear mixture of a small set of generic endmember spectra. Spatial mixing effects are removed by computing the (low‐variance) difference between the modeled and observed spectra, named the Mixture Residual (MR). The MR, a residual reflectance spectrum that is presumed to carry the subtler and variable signals of interest, is then leveraged as a source of signal. We illustrate the approach using three independent collections of reflectance spectra: synthetic composites computed from field measurements, NEON AOP airborne image compilations, and DESIS satellite data. The MR is found to discriminate between land cover versus plant trait signals, and to accentuate subtle absorption features. Mean band‐to‐band correlations within the visible, NIR, and SWIR wavebands decrease from 0.97, 0.94, and 0.97 to 0.95, 0.04, and 0.31 (respectively). The number of dimensions required to explain 99% of image variance increases from 4 to 13. We focus on vegetation as an illustrative example, but note that the concept can be extended to other classes of spectra and used as an input to other algorithms.