Terminal Spine Research Articles

Urogenital schistosomiasis, a leading cause of bladder squamous cell carcinoma

Abstract Introduction/Objective Schistosoma, genus of trematodes, commonly known as blood flukes are parasitic flatworms responsible for a group of infections in humans termed schistosomiasis and hundreds of millions of people are infected worldwide. In areas where Shistosoma is not endemic to the region most cases of bladder malignancy are of urothelial origin. In areas where Shistosoma is endemic bladder malignancy is more commonly squamous cell carcinoma. However, with increased travel, migration and tourism it is important to potentially consider Shistosoma infection in areas that are not typically considered endemic regions. Methods/Case Report A 62-year-old male presented to our hospital and revealed through an interpreter he has had 1.5 years hematuria with “pieces of meat or brown dirt”. Cystoscopy was performed and showed a large necrotic mass. CT scan showed an 8.2 cm exophytic bladder dome mass. TURBT was performed and a diagnosis of invasive high-grade urothelial carcinoma with squamous differentiation and with multiple Schistosoma organisms identified.The patient received Schistosoma treatment and was scheduled for radical cystectomy and ileal conduct. A few days before the scheduled procedure the patient presented to our emergency department with acute abdominal pain. He was subsequently diagnosed with progressive bladder cancer with new left malignant ureteral obstruction, which lead to hydronephrosis, DVT, abdominopelvic ascites and intraperitoneal bladder perforation. Abdominal fluid cytology showed atypical keratinizing squamous cells suspicous for malignancy and marked acute inflammation. Patient underwent cystectomy with ileal conduit and the specimen was sent to pathology for examination. Results (if a Case Study enter NA) NA Conclusion Gross examination showed an irregular perforated bladder. Microscopic examination showed a high grade urothelial carcinoma with squamous and sarcomatoid differentiation with detrusor muscle invasion. Additionally, Schistosoma organisms with a terminal spine and calcified eggs were identified. Some of the calcified egg material was engulfed by giant cells. The organisms are surrounded by a prominent eosinophilic infiltrate. Lymphovascular invasion is also noted. Schistosomiasis is associated with the development of squamous cell carcinoma of the bladder. It is important to maintain high oncological alertness for squamous cell carcinoma, particularly for patients arriving from endemic areas. Such as in our case, a patient from Somalia living in Kansas City.

Phenotypic analysis of mezcal agaves from the Central Valleys of Oaxaca

Objective: Mezcal agaves in the state of Oaxaca have a high economic value due to the demand for mezcal production; therefore, the purpose of this work was to assess and highlight the importance of morphological diversity within and among cultivated Agave species. Design/Methodology/Approach: A completely randomized experimental design with 25 treatments (populations) and 11 replicates (individuals) was implemented. The plant (individual) was the experimental unit and 19 morphological descriptors proposed by the National Seed Inspection and Certification Service (SNICS) were assessed. A total of 275 individuals of the Agave angustifolia, Agave karwinskii, Agave marmorata, Agave rhodacantha, Agave potatorum, Agave seemanniana, and Agave nussaviorum species were assessed using a multivariate analysis to determine their phenotypic variability and existing relationships. Results: The dendrograms for the Q-mode and R-mode were obtained by means of a cluster analysis, forming 4 groups based on the average linkage generated from the standardized BDM of the sampled species of the genus Agave. Four groups were formed using the k-means clustering method, in accordance with field observations and a review of the taxonomic bibliography. The first two principal components (PC) accounted for 66.4% of the total variation, according to the principal component analysis (PCA). For PC1 and PC2, the variables with the highest contribution were those related to leaf shape (Fh), size of the lateral spine (FEL), number of leaves (Nh), plant height (H), uniformity in the size of the lateral spines (UTE), and terminal spine shape (FET). Study Limitations/Implications: A comprehensive study requires taxonomic keys to identify species, subspecies, and even varieties of Agave. Additionally, molecular characterization is essential to understand the variability and phylogenetic relationships of these populations, subject to a phylogenetic analysis. Findings/Conclusions: Multivariate analysis techniques revealed that three species showed high phenotypic variability in the maturation stage under cultivation conditions. The A. potatorum and A. karwinskii species had a greater intra-population phenotypic variability, with significant differences within the same species. Agave marmorata showed no intra- or inter-population variability. Leaf texture (Txh) was the only variable that explained the variation within its group. This is a tall species whose diameter is larger than in the other species. The variables of the Mexican (Agave rhodacantha) group showed low correlation, as their behavior was highly dispersed. The variables obtained in the field from this group of populations must be meticulously assessed to identify the degree of correlation between the variables and to confirm the behavior of this group.

A new deep-sea Cristaphyes (Kinorhyncha: Allomalorhagida: Pycnophyidae) from the continental rise of South Island, New Zealand

ABSTRACT A new kinorhynch species, Cristaphyes microtubuliferus sp. nov., is described from the continental rise of South Island, New Zealand. The new species is recognised by the presence of very poorly developed lateral terminal spines, combined with its considerable trunk length, which can exceed 1 mm. In addition, the new species is characterised by morphologically dimorphic setae, i.e. the regular long and slender setae found in most pycnophyid species, and much shorter and more rigid ‘minute setae’. Besides the new species, the presence of two additional undescribed Cristaphyes species is reported. The material is insufficient to support formal species descriptions for the latter two, but short diagnoses are provided for the species. The description of Cristaphyes microtubuliferus sp. nov. brings the total number of described and identified kinorhynch species in New Zealand up to 19. With the new species included, the genus Cristaphyes now accommodates 26 species, of which only six are known from the southern hemisphere. LSID: urn:lsid:zoobank.org:pub: 77FEA91A-7684-4664-9DFC-470A245D9267

First record of the hippolytid shrimp Hippolyteaustraliensis (Stimpson, 1860) (Crustacea, Decapoda) from China.

Two specimens of the genus Hippolyte were examined from the caridean collections of the Marine Biological Museum, Chinese Academy of Sciences. These specimens were captured in the South China Sea in 1987. Detailed inspection revealed that their morphological features closely match the (re)descriptions of Hippolyteaustraliensis, especially in the presence of a long, distinct lateral carina on the rostrum, a dorsally unarmed rostral border, and four prominent terminal spines on the dactylus of the last three pereiopods. The discovery of Hippolyteaustraliensis in Chinese waters represents a significant expansion of its geographic distribution from the South Pacific to the North Pacific. Furthermore, an additional taxonomical report of H.australiensis is provided since its retrieval in 2001.

New species of Delicata (Molineidae: Anoplostrongylinae) parasite of Cabassous tatouay (Desmarest, 1804) from the Atlantic Forest, Rio de Janeiro, Brazil.

A new species of nematode parasite of the genus Delicata (Molineidae: Anoplostrongylinae) is described from the small intestine of a road-killed Greater Naked-tailed Armadillo Cabassous tatouay (Cingulata: Chlamyphoridae) on the BR-040 highway in Rio de Janeiro state, Brazil. The genus Delicata includes 13 species of parasitizing armadillos and anteaters distributed in Brazil, Argentina, and Trinidad and Tobago. The present species is distinguished from almost all species of Delicata by the longest length of the body, except for D. khalili and D. appendiculata. However, these can be distinguished from each other by the length of the spicules. The species that closely resembles, Delicata tatouay n. sp. is D. speciosa, but it can be distinguished by a robust branch from rays 2 and 3, rays 4 larger, and rays 8 longer compared to those of the new species. The new species is the only one with a tail, characterized by a terminal spine with rattlesnake tail-like transversal striations.

Two new species of Dichelyne Jägerskiöld, 1902 (Nematoda: Cucullanidae) parasitising marine fishes in Iraq.

Recent examinations of some marine fishes from off the southern coast of Iraq revealed the presence of two new species of Dichelyne (Dichelyne) Jägerskiöld, 1902 (Nematoda: Cucullanidae), both intestinal parasites, namely: D. (D.) lethrini n. sp. from the smalltooth emperor Lethrinus microdon Valenciennes (Lethrinidae, Acanthuriformes) and D. (D.) iraqiensis n. sp. from the humphead snapper Lutjanus sanguineus (Cuvier) (Lutjanidae, Acanthuriformes). Specimens are described and illustrated based on light and scanning electron microscopical examinations. Dichelyne (D.) lethrini n. sp. is mainly characterised by the length of spicules (522 µm), presence of a dorsal caecum, location of deirids at the level of the posterior end of oesophagus and the post-oesophageal position of the excretory pore, number (10 pairs) and distribution of caudal papillae in the male, and the presence of two minute terminal spines on the female tail tip. Dichelyne (D.) iraqiensis n. sp. differs from the species of the subgenus Dichelyne mainly based on the absence of caudal spines, the presence of a dorsal caecum, the length of spicules (789-884 µm), the location of deirids and the excretory pore approximately at the same level, and the size of eggs (72-78 × 42-51 µm). These new species infecting marine fishes in Iraq represent new geographic records of cucullanids of the subgenus Dichelyne from fishes in marine waters off Iraq.

A new mud shrimp of the genus Paragourretia Sakai, 2004, from the Gulf of Mexico (Crustacea: Decapoda: Ctenochelidae).

A new western Atlantic member of the ctenochelid genus Paragourretia Sakai, 2004 is described to accommodate a single unique specimen collected by dredge on muddy rubble substrates of the continental shelf in the southwestern Gulf of Mexico. Paragourretia sandrae n. sp. closely resembles Paragourretia biffari (Blanco Rambla & Liñero Arana, 1994), another regionally reported species for which photographs and additional illustrations are herein provided. While P. biffari and P. sandrae n. sp. are similar in habitus and known habitat to the more commonly encountered confamilial Dawsonius latispina (Dawson, 1967), the third maxillipeds in Paragourretia bear distinct exopods that are absent in Dawsonius Manning & Felder, 1991. As in eight other world congeners, P. sandrae n. sp. differs from both P. biffari and Dawsonius in lacking triangular ventrolateral projections on the sixth pleomere. It is further distinguished from P. biffari by lacking a distinctly incised notch in the anterodistal margin of the uropodal exopod and by absence of a median terminal spine on the telson. Additional sequence-quality specimens of P. sandrae n. sp. are required to clarify its relationships within the polyphyletic genus Paragourretia on a molecular genetic basis.

MORPHOLOGY OF THE DECAPODITE STAGES OF <i>ARGIS</i> <i>LAR</i> (OWEN 1839) (CARIDEA, CRANGONIDAE) FROM THE KRONOTSKY BAY, EASTERN KAMCHATKA

Two decapodite stages of the shrimp, Argis lar, family Crangonidae, from the plankton off the Kronotsky Bay, northwestern Pacific, eastern Kamchatka, are described. Сomparison of The available larvae were morphologically compared with decapodites of other species of this family with a shortened development. The first and second decapodite stages were similar in size and structural features of the pleon, carapace, antennae, mandibles, maxillae, pereiopods, and pleopods. Significant differences were found in the morphology of the telson, maxilliped, and antennules in the first and second decapodite stages of A. lar. In the second decapodite stage, the posterior median spine shifted to the middle of the carapace, the number of antennular and maxilliped endopod segments were increased, the epipodite appeared in the third maxilliped, the shape of the telson changed, the terminal spines in the telson disappeared, and the relative length of the angular setae were decreased. Kuro shrimps are thought to have three to five larval stages.

Amplectobeluid Radiodont Guanshancaris gen. nov. from the Lower Cambrian (Stage 4) Guanshan Lagerstätte of South China: Biostratigraphic and Paleobiogeographic Implications.

Radiodonta, an extinct stem-euarthropod group, has been considered as the largest predator of Cambrian marine ecosystems. As one of the radiodont-bearing Konservat-Lagerstätten, the Guanshan biota (South China, Cambrian Stage 4) has yielded a diverse assemblage of soft-bodied and biomineralized taxa that are exclusive to this exceptional deposit. "Anomalocaris" kunmingensis, the most abundant radiodont in the Guanshan biota, was originally assigned to Anomalocaris within the Anomalocarididae. Despite this taxon being formally assigned to the family Amplectobeluidae more recently, its generic assignment remains uncertain. Here, we present new materials of "Anomalocaris" kunmingensis from the Guanshan biota, and reveal that the frontal appendages possess two enlarged endites; all endites bear one posterior auxiliary spine and up to four anterior auxiliary spines; three robust dorsal spines and one terminal spine protrude from the distal part. These new observations, allied with anatomical features illustrated by previous studies, allow us to assign this taxon to a new genus, Guanshancaris gen. nov. Brachiopod shell bearing embayed injury and incomplete trilobites, associated with frontal appendages in our specimens, to some extent confirm Guanshancaris as a possible durophagous predator. The distribution of amplectobeluids demonstrates that this group is restricted to Cambrian Stage 3 to Drumian, and occurs across South China and Laurentia within the tropics/subtropics belt. Moreover, the amount and abundance of amplectobeluids evidently decreases after the Early-Middle Cambrian boundary, which indicates its possible preference for shallow water, referring to its paleoenvironmental distribution and may be influenced by geochemical, tectonic, and climatic variation.

Redescription of the mole crab Emerita portoricensis Schmitt, 1935 (Crustacea: Decapoda: Hippidae), based on Caribbean populations from Puerto Rico, Belize, Costa Rica, and Panama

The mole crab Emerita portoricensis Schmitt, 1935 was originally described solely on the basis of few key characters that were not precisely defined, giving reason to question subsequent reports of its distribution. The present study, prompted by recent collections documenting coloration in life, undertakes a comprehensive redescription of the species based on specimens of varied sizes from Puerto Rico, Belize, Costa Rica, and Panama. Collections from the northern Caribbean that at first take appear to represent a northernmost record of E. brasiliensis Schmitt, 1935 or southernmost occurrence of E. talpoida (Say, 1817), may be assignable E. portoricensis as now recognized. Among western Atlantic species, E. portoricensis and E. benedicti have to date been considered to have the dactylus of the first pereopod terminally subacute or sharply pointed, which purportedly separates them from E. brasiliensis and E. talpoida, western Atlantic species in which this article is terminally rounded. However, in E. portoricensis this character varies with specimen size and the magnification at which the distal extreme of the dactylus is examined, being rounded to varying degrees in all but the largest specimens. Even in sexually mature specimens of less than maximum size, this rounded tip is armed by a minute corneous spine in E. portoricensis, although it is less prominent than the terminal spine on the consistently more acute dactylus of E. benedicti at all adult sizes. Also, the carapace color in live specimens of E. portoricensis, as documented for specimens collected in both Belize and Panama, differs from that of E. brasiliensis, E. talpoida, and E. benedicti by typically including longitudinal and diagonal dark bars of olive brown on the branchial regions and a light longitudinal bar marking the posterior quarter of the median line. Posterior to the cervical groove, fine rugae of the carapace that form broken transverse lines are at most little diminished across the mid-dorsal longitudinal line in E. portoricensis and E. benedicti, somewhat more broken in E. brasiliensis, and distinctly diminished to all but absent at the midline in E. talpoida. Previously reported BINs in the Barcode of Life database include sequenced specimens from Costa Rica herein accepted as E. portoricensis. We exclude populations from Brazil that have been mis-assigned to E. portoricensis.

New Echinoderes (Kinorhyncha: Cyclorhagida) from Mexico: Molecular barcoding demonstrate species delimitation between highly similar morphospecies

Two new species of Echinoderes are described from Mexico. Echinoderes abeli sp. nov. is described from the archipelago Islas Marias in the Mexican Pacific Ocean west coast. The species is characterized by very short and stout lateral terminal spines, middorsal spines on segments 4 to 8 and lateroventral spines on segments 6 to 9, lateroventral tubes on segment 2 and 5, midlateral tubes on segment 4, and sublateral ones on segment 8, and in laterodorsal positions on segment 10, as well as glandular cell outlets type 2 in subdorsal positions segment 2. The second new species, Echinoderes wilberti sp. nov. is described from the Caribbean coast in Yucatan. The new species show great resemblance with another species, Echinoderes horni, that occurred at the same locality. The only notable difference is a pair of subdorsal tubes on segment 2, present in E. wilberti sp. nov. only. However, barcoding of COI demonstrated the two morphospecies differed genetically more than 8%. This result shows that small morphological differences might be supported by much more conspicuous molecular differences. Based on the high level of morphological similarity between E. wilberti sp. nov. and E. horni, as well as a third Caribbean species, Echinoderes parahorni, a species group, the E. horni group, is proposed to accommodate this potentially monophyletic group of species.This article is registered at www.zoobank.org under urn: lsid: zoobank.org:pub: 5AE5C820-C922-492B-83F3-66C957E53453.

Expanding the Echinoderes coulli group (Kinorhyncha: Cyclorhagida) with a new species from the Chuuk Islands, Micronesia

A new species of Echinoderes is described from the Chuuk Islands, Micronesia. Echinoderes inaequalis sp. nov. clearly belongs to the Echinoderes coulli species group due to the presence of enlarged sieve plates, reduced trunk spines (excluding the terminal series) and a hairy habitus. E. inaequalis sp. nov. is characterised by the presence of a minute middorsal spine on segment 4; unpaired subdorsal tubes in alternating positions on segments 5–7; lateroventral tubes on segments 5, 7–9, midlateral tubes on segment 8, sexually dimorphic laterodorsal tubes on segment 10; numerous glandular cell outlets type 2 located on segments 2, 4–8; and very short and stout terminal spines (ca. 12% of the trunk length). The subdorsal unpaired tubes of segments 5–7 show intraspecific variation. E. inaequalis sp. nov. increases the count of the E. coulli group to 23 species.

Revision of an Arctic kinorhynch species: Echinoderes svetlanae and E. tubilak are junior synonyms of E. remanei

The kinorhynch species Echinoderes remanei (Blake, 1930) is redescribed herein, based on the material housed in the National Museum of Natural History, Smithsonian Institution in Washington D.C as well as specimens collected at several other sites in the Northern Hemisphere. Echinoderesremanei is characterized by the presence of middorsal spines on segments 4 to 8 and lateroventral spines on segments 6 to 9; four pairs of glandular cell outlets type 2 on segment 2 (subdorsal, laterodorsal, sublateral, and ventrolateral), and one pair on segment 4 (subdorsal), 5 (midlateral), 8 (sublateral) and 10 (laterodorsal); lateroventral tubes on segment 5; and by sexual dimorphism expressed in lateral terminal spine lengths (in females, the lateral terminal spines are about half as long as those in males). The study also reveals that the two other species, Echinoderes tubilak Higgins & Kristensen, 1988 and Echinoderes svetlanae Adrianov, 1999 in Adrianov & Malakhov (1999) are conspecific with E. remanei. Therefore, E. tubilak and E. svetlanae are proposed as junior synonyms of E. remanei and are synonymized with E. remanei (Blake, 1930).

Dracophyes cepedai gen. et sp. nov., a new Dracoderidae genus and species (Kinorhyncha: Allomalorhagida) from the Peru Basin

A new Dracoderidae genus, Dracophyes gen. nov., and a new species Dracophyes cepedai sp. nov. from the Peru Basin are described. The new genus is characterized by the presence of acicular spines showing conspicuous intracuticular structures on segments 1–9 (either anchor for the dorsal or groove-like for the lateral series), alternating laterally displaced dorsal spines, becoming more lateral towards the posterior segments, males with two pairs of minute, poorly sclerotized penile spines, and its conspicuous obovate trunk shape. The new genus accommodates three species: Dracophyes toyoshioae comb. nov., Dracophyes chaac comb. nov. and D. cepedai sp. nov. The latter was collected in abyssal depths in the Peru Basin (East Pacific Ocean) and is recognised by having dorsal acicular spines on segments 1 to 9, alternating laterally displaced from the middorsal line, ventral acicular spines in ventrolateral position on segment 1, lateroventral position on segments 2–4 and 6–9, and in lateral accessory position on segment 5, tubes in lateroventral position on segment 5 and in lateral accessory position on segments 2 and 8, type 2 glandular cell outlets in lateroventral position on segment 10 and extremely long lateral terminal spines. Moreover, with the erection of the new genus, emended diagnoses for the family Dracoderidae and the former single genus within the family, Dracoderes, are proposed.

A new species of Tharybis (Crustacea: Copepoda: Calanoida: Tharybidae) from northern Taiwan

A new species Tharybis kueishanensis sp. n. (Copepoda: Calanoida: Tharybidae) is described from the inshore surface waters of Kueishan Island off northern Taiwan, which is the first report of Tharybis from the China Seas. Its female is distinguished from the other species of Tharybis by the total body length (1.89-2.12 mm) larger than the other species, the shape of the genital double-somite, which is slightly asymmetrical with a small notch on the distal margin of the right side, and the length of the inner terminal spine of leg 5, which is about 1.5 times of the terminal spiniform projections. The male is distinguished by the presence of 3 triangular projections at the inner middle margin and the tip of the distal segment of the right leg 5.

Agave infiernilloensis (sect. Choritepalae, Asparagaceae), a gigantic new species from the Balsas Basin in Western Mexico

We describe and illustrate Agave infiernilloensis, a new species of the section Choritepalae (Agavoideae, Asparagaceae). It is morphologically similar to A. gypsicola from which it differs by having larger rosettes and leaves, shorter terminal spine, shorter and recurving inflorescence, more flowers per node, as well as longer flowers, ovary, and filaments. We assessed the species as Endangered (EN) according to the IUCN criteria. We provide a distribution map and a key to the choritepalous species of Agave.

Transgenerational changes in Daphnia magna under radio frequency radiation in the juvenile and puberty period

Purpose To analyze the results of direct and transgenerational effects of radio frequency electromagnetic fields (RF-EMF) on the model organism of crustaceans Daphnia magna. Materials and methods D. magna were chronically exposed at 900 GHz EMF with an energy flux density (EFD) of about 1 mW/cm2 in the juvenile and pubertal periods of their ontogenesis. The cytotoxicity of exposure as well as survival, fertility and teratogenic effect of directly exposed daphnids and their progeny across three generations were analyzed. Results and conclusions The results of our study show that exposure of RF-EMF at juvenile period can significantly affect the fertility and size of irradiated daphnids and their offspring of the first generation. The decrease in fertility may be associated with a cytotoxic effect on the cells of irradiated animals. The reduction in the size of the terminal spine and the body of individuals is an indicator of the negative impact of radiation on the protective strategy of the crustacean population. The reproductive process is restored by the second generation. The results of our study provide further insights into the possible mechanisms underlying the in vivo effects of RF-EMF.

Taxonomic study on the genus Stenochironomus Kieffer from the Baishanzu Nature Reserve, China (Diptera, Chironomidae).

During the summer of July to September 2020, a biodiversity survey on Chironomidae of Baishanzu Nature Reserve, China was made. In total, five Stenochironomus taxa/species were discovered, of which two belong to undescribed species and one (S.okialbus Sasa, 1990) is reported for the first time from China. The male adults of two new species are described and illustrated. Stenochironomusannulus Song & Qi sp. nov. is distinguished in having a wing with two dark spots restricted to the fork area of FCu and RM, the mid- and hind-femur each with a brown annulus, and the inferior volsella with two setae and one strong terminal spine. Stenochironomusbaishanzuensis Song & Qi sp. nov. is distinguished by a combination of characters: a single dark spot on the middle part of the wing, fore legs brown to dark brown except for the basal 3/4 of femur, and the inferior volsella with four long setae and one stout terminal spine. The neighbour-joining tree based on public COI barcodes formed distinct clades with clear support for the new species. An updated key to known male adults of Stenochironomus from China is also provided.

Setaphyes algarvensis sp. nov., the first description of an allomalorhagid mud dragon (Kinorhyncha: Allomalorhagida) from Portugal (Eastern Atlantic Ocean)

The kinorhynch fauna from Portugal has been explored, yielding a new species of the genus Setaphyes (Kinorhyncha: Allomalorhagida). This is the first description of an allomalorhagid species from Portugal. Specimens of the new species were collected at a subtidal muddy beach in Alvor, a village located in the southernmost region of Portugal. Setaphyes algarvensis sp. nov. may be distinguished from its congeners by a unique arrangement of the setae: paired paradorsal setae on segments 2–7 and 9, paradorsal seta on segment 8 unpaired, laterodorsal setae on segments 2–3 and 6–9 in males and 2–9 in females, paralateral setae on segment 1, lateroventral setae on segments 2–10 (two pairs on segment 5), ventrolateral setae on segment 1 in males and 1–3 in females, and ventromedial setae on segments 3–9 in males and 4–9 in females. The diagnostic features of Setaphyes algarvensis sp. nov. are discussed from a comparative perspective with the congener species. Additionally, morphometric analyses of selected features, namely the total trunk length and the relation between the total trunk length and the length of lateral terminal spines, turned out to be useful to distinguish between the new species and Setaphyes kielensis (its most similar congener).

First trigonotarbid arachnids from the Pennsylvanian of Indiana and Oklahoma

AbstractA new specimen of the arachnid order Trigonotarbida is described from the Middle Pennsylvanian (lower Desmoinesian) Shelburn Formation of Indiana, which has previously yielded the remains of a phalangiotarbid. Two new trigonotarbid arachnid specimens are also described from the Middle Pennsylvanian (Desmoinesian) Senora Formation of Oklahoma. These are the first trigonotarbid specimens reported from Indiana and Oklahoma. The Indiana trigonotarbid belongs to the Eophrynidae, as indicated by distinct features such as the large tubercles on the dorsal surface of the opisthosoma and two pairs of terminal opisthosomal spines. This specimen is the first arachnid fossil to be imaged using a Multistripe Laser Triangulation scanner. The heavy dorsal tuberculation, lobed and subtriangular carapace, rounded clypeus, lack of terminal opisthosomal spines, and rounded opisthosoma on Oklahoma specimen FMNH PE 56932 indicate it belongs to the genusAphantomartus, in Aphantomartidae. The other Oklahoma specimen, FMNH PE 56955, possesses opisthosomal tergites that are divided into five plates longitudinally as well as a subquadrate carapace, which identify it as a member of Anthracomartidae; its rounded opisthosomal margin shows it to belong to the genusAnthracomartus.