The present study was designed to explore furthermore the relationship between abnormalities in mice whichwere used as a model to study the (NTDs) in human beings and retinoic acid. The study was conducted onthe Swiss white mice, with 40 females and 10 males. Females that have the vaginal plug are isolated inseparate plastic cages to study the teratogenic effect of retinoic acid in the concentration of 15, 25, 35 or45 mg/kg live body weight which were administered intraperitonially. Also, folic acid was used with dose35mg/kg to test the preventive effect against neural tube defects. Retinoic acid doses of 15 mg/kg, 25 mg/kg and 35 mg/kg caused different abnormalities in percent of 20% including (Small size with unclear face,NTDs), 32% including (NTDs, prolonged embryos and deformation eye and ears), 68% including (NTDs,prolonged embryos and deformation eye and ears and small size) respectively. While the dose of 45 mg/kgcaused 100% reabsorption.