Temperate Climate Research Articles

The impact of temperature on non-typhoidal Salmonella and Campylobacter infections: an updated systematic review and meta-analysis of epidemiological evidence

As temperatures rise, the transmission and incidence of enteric infections such as those caused by Salmonella and Campylobacter increase. This study aimed to review and synthesise the available evidence on the effects of exposure to ambient temperatures on non-typhoidal Salmonella and Campylobacter infections. A systematic search was conducted for peer-reviewed epidemiological studies published between January 1990 and March 2024, in PubMed, Scopus, Embase, and Web of Science databases. Original observational studies using ecological time-series, case-crossover or case-series study designs reporting the association between ambient temperature and non-typhoidal Salmonella and Campylobacter infections in the general population were included. A random-effects meta-analysis was performed to pool the relative risks (RRs) per 1°C temperature increase, and further meta regression, and subgroup analyses by climate zone, temperature metrics, temporal resolution, lag period, and continent were conducted. The Navigation Guide systematic review methodology framework was used to assess the quality and strength of evidence. The study protocol was registered with the International Prospective Register of Systematic Reviews (PROSPERO). Out of 3472 results, 44 studies were included in this systematic review encompassing over one million cases each of Salmonella and Campylobacter infections. Geographically, the 44 studies covered 27 countries across five continents and most of the studies were from high income countries. The meta-analysis incorporated 23 Salmonella studies (65 effect estimates) and 15 Campylobacter studies (24 effect estimates). For each 1°C rise in temperature, the risk of non-typhoidal Salmonella and Campylobacter infections increased by 5% (RR: 1.05, 95% CI: 1.04-1.06), and 5% (RR: 1.05, 95% CI: 1.04-1.07%), respectively, with varying risks across different climate zones. The overall evidence was evaluated as being of "high" quality, and the strength of the evidence was determined to be "sufficient" for both infections. These findings emphasise the relationship between temperature and the incidence of Salmonella and Campylobacter infections. It is crucial to exercise caution when generalising these findings, given the limited number of studies conducted in low and middle-income countries. Nevertheless, the results demonstrate the importance of implementing focused interventions and adaptive measures, such as the establishment of localised early warning systems and preventive strategies that account for climatic fluctuations. Furthermore, our research emphasises the ongoing need for surveillance and research efforts to monitor and understand the changing dynamics of temperature-related enteric infections in the context of climate change. Australian Research Council Discovery Projects grant (ARC DP200102571) Program.

Thermal Performance of Novel Eco-Friendly Prefabricated Walls for Thermal Comfort in Temperate Climates

The global threat of climate change has become increasingly severe, with the efficiency of buildings and the environment being significantly impacted. It is necessary to develop bioclimatic architectural systems that can effectively reduce energy consumption while bringing thermal comfort, reducing the impact of external temperatures. This study presents the results of a real-scale experimental house prototype, MHTITCA, using a unique design composed of novel eco-friendly prefabricated channel walls filled with a blend of soil, sawdust, and cement for walls and roofs. The experimental analysis performed in this study was based on dynamic climatology. A solar orientation chart of the place was constructed to identify the solar radiation intensity acting on the house. Measurements of roof surface temperatures were conducted to determine temperature damping and temperature wave lag. Monthly average temperature and direct solar radiation data of the site were considered. Compared to other alternative house prototypes, the system maximizes thermal comfort in high-oscillation temperate climates. Temperature measurements were taken inside and outside to evaluate the thermal performance. A thermal insulating layer was added outside the wall and the envelope to improve the prototype’s thermal comfort and reduce the decrement factor even more. This MHTITCA house prototype had 85% thermal comfort, 0% overheating, and 15% low heating. This eco-friendly prototype design had the best thermal performance, achieving a thermal lag of twelve hours, a reduced decrement factor of 0.109, and preventing overheating in areas with high thermal fluctuations. Comparatively, the other prototypes examined provided inferior thermal comfort. The suggested MHTITCA system can be an energy-saving and passive cooling option for thermal comfort in low-cost houses in temperate climates with high thermal oscillations in urban or rural settings.

Public health effectiveness of cooling centres in adverse hot weather events: a systematic review

Abstract Background The 2022 heatwave in England highlighted the need for effective public health interventions like cooling centres in temperate climates where built-in air conditioning is uncommon. Aim To determine the impacts of cooling centres on heat-related health outcomes for users in temperate climates, assessing their effectiveness and identifying barriers to usage. Methods This systematic review analysed literature sourced from MEDLINE, Embase, Web of Science, CENTRAL, and Google Scholar from January 2010 to February 2023. Criteria for inclusion focused on studies evaluating health outcomes related to cooling centre use. Screening and quality assessments were independently conducted using the Mixed Methods Appraisal Tool. Results The review included six studies: three quantitative, three qualitative. Two were evidence synthesis of prior studies. No studies conclusively measured health and wellbeing outcomes directly after cooling centre use; instead, research emphasised user demographics, barriers, and facilitators. Common barriers included accessibility, operational hours, and public perceptions, which often misalign with user needs. Successful implementations were noted in centres integrated within community facilities like libraries or community centres. One modelling study suggested a number needed to treat to avoid one heat-related death of 1 million. The relevance of this is unclear as it was in a hotter climate with greater prevalence of air conditioning than in England. Conclusions While direct health benefits in cooler climates like England are poorly documented, cooling centres play an essential role in comprehensive heat response strategies. They offer potential social benefits and provide a cost-effective alternative to urban redesign. Future studies should aim to directly measure health outcomes and refine operational strategies to maximise benefits. Key messages • Cooling centres are crucial in comprehensive heat response strategies, yet direct evidence in cooler climates like England remains limited. • Integration with existing community services may significantly enhance cooling centre utilisation and effectiveness.

Assessment of background dose rate on non-human biota in a Mediterranean terrestrial ecosystem.

The assessment of radiological impact to the environment is usually carried out by the dose rate estimation to hypothetical entities named Reference Animals and Plants (RAPs). There are many codes to carry out this assessment, which requires the definition of a scenario and using site-specific transfer parameters when possible. Transfer parameters present a geographical bias, as they are mostly derived from temperate and arctic climate datasets, but there is a scarcity of data for Mediterranean climates. In this study, a terrestrial Mediterranean scenario was defined using the distribution of activity concentrations of anthropogenic (90Sr, 137Cs) and naturally occurring radionuclides (40K, 210Pb, 210Po, 226,228Ra, 235,238U, 232Th) in Cáceres province (Spain). Site-specific transfer factors, CRwo-media, defined as the ratio between the concentration in the whole organism and the medium (soil in this case) were considered. Dose rate assessments for terrestrial RAPs were carried out using Tier 3 in ERICA Tool, ranging 0.23-3.73µGy/h which is below the screening level of 10µGy/h. Therefore, no harmful effects are expected to occur. Internal dose rate predominates over external one because the main contributors are naturally occurring radionuclides (in most cases 40K, 226,228Ra, 210Pb, 210Po), which are mostly α-emitting radionuclides. These results can be used for the evaluation of other radiological and nuclear installations in Mediterranean climates, as they set the background dose rate.

The Developmental Cycle of Spirodela polyrhiza Turions: A Model for Turion-Based Duckweed Overwintering?

Duckweeds are widely distributed small, simply constructed aquatic higher plants (the Lemnaceae) found on quiet freshwater surfaces. Species inhabiting temperate climates may have to cope with long periods of severe cold during the winter season. Several duckweeds form compact resting structures from the assimilatory fronds of the growing season that can bridge inhospitable conditions in a quiescent state. Of these, turions separate from the mother fronds and overwinter on the water body bottom in a dormant state. They can surface, germinate, and sprout to resume active growth upon warming in the spring. The turions of the largest duckweed, Spirodela polyrhiza, have been intensively examined as to ultrastructure, the factors governing their formation and release from dormancy, and the signals driving their germination and sprouting and the accompanying starch degradation. Comparative transcriptomics of assimilatory fronds and dormant turions are revealing the molecular features of this developmental cycle. The results illustrate an elegant sequence of reactions that ensures aquatic survival of even severe winters by frost avoidance in a vegetative mode. Since little is known about other duckweed resting fronds, the S. polyrhiza turion developmental cycle cannot be considered to be representative of duckweed resting fronds in general but can serve as a reference for corresponding investigations.

Effects of High Tunnel Soil Solarization on Sclerotinia sclerotiorum in the Temperate Climate of Central Kentucky.

Diseases caused by Sclerotinia spp. can affect a wide range of plants, including vegetables, with yield losses ranging from 10 to 50%. Sclerotinia diseases can be especially problematic in high tunnels where high-value vegetable crops are planted in early spring to extend the growing season, achieve earlier harvest, and bring higher profits. Fungicide applications and crop rotations are limited due to product application restrictions and constraints on time, crop resistance, and profitability. Soil solarization is a cultural management method that uses transparent polyethylene to raise soil temperatures via solar irradiation to kill pathogens, pests, and weeds. A two-year study was conducted in a Kentucky high tunnel to determine the maximum temperature potential of solarization at various soil depths at different durations during different seasons and to identify temperatures at which S. sclerotiorum sclerotia lose viability. The experiment included solarization treatments of 2, 4, and 6 weeks and a non-solarized control implemented in spring, summer, and fall. Sclerotia and temperature data loggers were buried at 5.1, 10.2, and 15.2 cm soil depths. The number of hours at which soil temperatures reached ≥ 40 °C was greatest in summer in both years, followed by fall, and then spring. The highest average daily maximum soil temperature reached was 48.9°C, which occurred during the summer 6-week solarization in Year 1. The viability of buried sclerotia was overall lower in solarized treatments compared to non-solarized treatments in both years. In general, the 2-week solarization treatment had significantly higher percent sclerotial germination than the 4-week and 6-week treatments, which were not significantly different from one another. The viability of sclerotia was progressively higher with burial depth. In both years, sclerotia germination was significantly lower in summer compared to spring and fall.

Cultivation and Potential for Biomass Production for Energy and Seed Purposes of Tall Wheatgrass (Agropyron elongatum (Host) Beauv.) Under Sandy Soil and Temperate Climate Conditions

An experiment was conducted to analyse the potential for obtaining biomass for energy purposes and tall wheatgrass (TWG) seeds grown under conditions of varying pre-sowing fertilisation with compost and mineral fertilisation with nitrogen on sandy soils. Field trials were conducted between 2012 and 2015. The study factors were compost from municipal green areas with I-doses of 0, 10 and 20 Mg∙ha−1 added before sowing and nitrogen II-doses of 0, 40, 80 and 120 kg∙ha−1 added each year in the form of ammonium nitrate. During the experiment conducted on sandy soils, a favourable effect of fertilisation on the morphological parameters of above-ground vegetative and generative parts was found. The experiment resulted in high dry matter yields (DMYs) in the range of 9.08–31.38 Mg∙ha−1 and high seed yields (SYs) (635 kg∙ha−1 to as much as 2397 kg∙ha−1), which depended on the applied fertilisation variant. The applied levels of compost fertilisation had a positive effect on the obtained dry matter yields (DMYs) and SY. Analysing the effect of the applied doses of mineral nitrogen fertilisation (40, 80 and 120 kg∙ha−1) on the dry matter yield of TWG under sandy soil conditions, it should be noted that this factor significantly increased the DMY and SY in all years of the study. At the same time, the response of plants to this factor of the study over the years varied and depended on weather conditions. The high energy yield (192.50 GJ∙ha−1–408.93 GJ∙ha−1) closely related to the high DMY indicates the high suitability of TWG as a new grass species under temperate climate conditions grown for biomass energy production and the possibility to harvest seeds when adequately cultivated.

Cover crop characteristics modulate amount and timing of nitrogen supply of fertilized sugar beet (Beta vulgaris L.) in temperate climate

For temperate climate, there is little information on the effects cover crops grown in fall (CCs) on the nitrogen (N) supply for next year’s sugar beet (SB). Four field trials were conducted on silty soils to establish the CC N effect (Neff) compared to bare fallow separately for the periods sowing-summer and summer-autumn harvest. Biomass characteristics of radish (Raphanus sativus L.), spring vetch (Vicia sativa L.), saia oat (Avena strigosa Schreb.), and winter rye (Secale cereale L.) CCs before winter, soil mineral nitrogen in spring (SMN), and SB N accumulation and sugar yield (SY) were measured. In the period sowing-summer, characterized by a high SB N demand, Neff of overwintering, high biomass yielding rye CC was negative up to -50 kg N ha-1 at three site/years and varied around zero for the other CCs except vetch, for which Neff was positive. At SB autumn-harvest, Neff was negative up to -100 kg N ha-1 except for vetch in one trial. SY was lowest after rye CC. Regression analyses indicated a negative impact of CC biomass, C:N ratio and the difference in SMN between fallow (high SMN) and CCs (low SMN) on Neff. To conclude, if CCs yield a high amount of biomass surviving until spring and thus remove SMN from the soil which otherwise remains available for SB, early season mineralization of CC biomass N can be too low to ensure a N supply sufficient for maximum SB yield. Choosing leguminous CCs or early termination of CCs might alleviate this constraint.

Solar Species: Energy Optimization of Urban Form Through an Evolutionary Design Process

This paper proposes design guidelines to enhance energy efficiency and energy generation potential in active solar buildings. Additionally, it presents a variety of optimized urban forms characterized by attributes such as shape, layout, and number of buildings on the plot. These urban configurations are classified into solar species, each associated with a distinct range of high passive and active solar potential. These results were achieved by developing and applying a simulation-driven, multi-objective optimization technique for the early-stage design of a residential building cluster in a temperate climate. This method leverages both passive and active energy indicators, employing a genetic algorithm to identify optimal forms that maximize active solar potential while also minimizing operational energy demand. The approach utilizes a parametric modelling routine that relies on vertical cores and horizontal connections to produce design iterations featuring irregular geometry, while ensuring structural continuity and means of egress. The findings reveal a significant variability in onsite energy generation, with optimized solutions differing by a factor of 2.5 solely based on shape, underscoring the critical role of active solar potential. Taken together, these results hint at the descriptive and predictive capabilities of these solar species, making them a promising heuristic model for characterizing urban form in relation to energy performance.

A transgenerational study on the effect of granddam conception month on granddaughter test day records for milk production traits in Italian Simmental cattle

The consequences of climate change are becoming a relevant issue also for the livestock industry in temperate countries. Transgenerational studies outlined the effect of the ancestors’ calving season, considered as an indicator of the period during which pregnancy occurs, on their female progeny performance. The aim of this study was to evaluate the effect of great granddam (F0) calving month on their great granddaughters (F3) Test Day (TD) records for milk production. A total of 414,069 TD records of milk, fat, and protein yields from 27,224 Italian Simmental F3 cows was analysed with a mixed linear model with year of calving, parity, age at parity, F0 calving month, days in milk class, the interactions between DIM and F0 calving month and between parity and F0 calving month, F3 calving date and the random effects of herd-test date and animal. The F0 calving month significantly influenced F3 performance for the three considered traits; largest F3 TD records were obtained for F0 calvings occurring in October, smallest for February. The largest milk production of F3 cows were observed when their F1 conceptions occurred in winter. Results were confirmed by a further analysis considering the calving seasons of F0, F1 and F2. The effect of F0 calving month (associated with the conception of F1) on milk production decreased as F3 parity increased. Results of this study suggest that the period of ancestor pregnancy and conception affects milk production traits of further generations also in temperate climates.

Cross-continental comparison of plant reproductive phenology shows high intraspecific variation in temperature sensitivity

Abstract The success of plant species under climate change will be determined, in part, by their phenological responses to temperature. Despite the growing need to forecast such outcomes across entire species ranges, it remains unclear how phenological sensitivity to temperature might vary across individuals of the same species. In this study, we harnessed community science data to document intraspecific patterns in phenological temperature sensitivity across the multicontinental range of six herbaceous plant species. Using linear models, we correlated georeferenced temperature data with 23,220 plant phenological records from iNaturalist to generate spatially-explicit estimates of phenological temperature sensitivity across the shared range of species. We additionally evaluated the geographic association between local historic climate conditions (i.e. mean annual temperature and interannual variability in temperature) and the temperature sensitivity of plants. We found that plant temperature sensitivity varied substantially at both the interspecific and intraspecific level, demonstrating that phenological responses to climate change have the potential to vary both within and among species. Additionally, we provide evidence for a strong geographic association between plant temperature sensitivity and local historic climate conditions. Plants were more sensitive to temperature in hotter climates (i.e., regions with high mean annual temperature), but only in regions with high interannual temperature variability. In regions with low interannual temperature variability, plants displayed universally weak sensitivity to temperature, regardless of baseline annual temperature. This evidence suggests that pheno-climatic forecasts may be improved by accounting for intraspecific variation in phenological temperature sensitivity. Broad climatic factors such as mean annual temperature and interannual temperature variability likely serve as useful predictors for estimating temperature sensitivity across species’ ranges.

The effect of spermidine supplementation on polyamine metabolism and Fusarium infection in Linum usitatissimum

Flax (Linum usitatissimum) is a valuable industrial crop in temperate climate zones. However, the prevalence of fungal diseases, particularly those caused by Fusarium oxysporum sp. linii, poses a substantial threat, leading to significant crop losses and diminished interest in flax cultivation. In this study, flax plants were treated with spermidine and subsequently infected with Fusarium oxysporum to investigate multiple aspects: (1) the uptake of exogenous spermidine by the plants, (2) the impact of this uptake on the levels of other polyamines and the expression of polyamine biosynthesis genes, and (3) the effects of fungal infection on these parameters. The results demonstrated that flax plants effectively absorb spermidine, leading to substantial changes in the levels of polyamines and the expression of related genes in both roots and shoots. Notably, spermidine treatment resulted in significant alterations in the levels of putrescine and spermine, as well as in the transcript levels of key genes involved in polyamine biosynthesis. Moreover, Fusarium oxysporum infection itself induced changes in polyamine content and gene expression, which varied between root and shoot tissues. When spermidine-treated plants were infected with Fusarium oxysporum, a more complex response was observed, characterized by modifications in polyamine levels and gene expression that suggest an enhanced defense mechanism against the pathogen. These findings indicate that polyamine treatment not only affects the infection process but also modulates the plant's internal polyamine metabolism and gene expression, contributing to an improved resistance to fungal attack. This study highlights the potential of spermidine as a protective agent in flax and provides a foundation for further exploration of polyamine-related defense mechanisms.

GWAS for Drought Resilience Traits in Red Clover (Trifolium pratense L.)

Red clover (Trifolium pratense L.) is a well-appreciated grassland crop in temperate climates but suffers from increasingly frequent and severe drought periods. Molecular markers for drought resilience (DR) would benefit breeding initiatives for red clover, as would a better understanding of the genes involved in DR. Two previous studies, as follows, have: (1) identified phenotypic DR traits in a diverse set of red clover accessions; and (2) produced genotypic data using a pooled genotyping-by-sequencing (GBS) approach in the same collection. In the present study, we performed genome-wide association studies (GWAS) for DR using the available phenotypic and genotypic data. Single nucleotide polymorphism (SNP) calling was performed using GBS data and the following two red clover genome assemblies: the recent HEN-17 assembly and the Milvus assembly. SNP positions with significant associations were used to delineate flanking regions in both genome assemblies, while functional annotations were retrieved from Medicago truncatula orthologs. GWAS revealed 19 significant SNPs in the HEN-17-derived SNP set, explaining between 5.3 and 23.2% of the phenotypic variation per SNP–trait combination for DR traits. Among the genes in the SNP-flanking regions, we identified candidate genes related to cell wall structuring, genes encoding sugar-modifying proteins, an ureide permease gene, and other genes linked to stress metabolism pathways. GWAS revealed 29 SNPs in the Milvus-derived SNP set that explained substantially more phenotypic variation for DR traits, between 5.3 and 42.3% per SNP–trait combination. Candidate genes included a DEAD-box ATP-dependent RNA helicase gene, a P-loop nucleoside triphosphate hydrolase gene, a Myb/SANT-like DNA-binding domain protein, and an ubiquitin–protein ligase gene. Most accessions in this study are genetically more closely related to the Milvus genotype than to HEN-17, possibly explaining how the Milvus-derived SNP set yielded more robust associations. The Milvus-derived SNP set pinpointed 10 genomic regions that explained more than 25% of the phenotypic variation for DR traits. A possible next step could be the implementation of these SNP markers in practical breeding programs, which would help to improve DR in red clover. Candidate genes could be further characterized in future research to unravel drought stress resilience in red clover in more detail.

Holocene palaeoecological changes in a transitional climate zone of western-central Mexico: The pollen and non-pollen palynomorphs record

The Alberca de Tacámbaro (AT) is located at a transitional zone between tropical and temperate climates at the core of the North American Monsoon area. This location features a mosaic vegetation comprising pine-oak forests and tropical dry forests. Currently there is no information regarding the dynamics or evolution during the Holocene of these vegetation types. The palynological record of the last 9400 years BP preserved in the lacustrine sequence of the AT was used to document changes in the composition of terrestrial and aquatic communities, evenness, and rate of change. Pinus and Quercus forests dominated the terrestrial palynological record, but mesophytic forests were also recorded throughout the sequence. Four tropical dry forest taxa assemblage expansions were identified at 9200 to 8420 yr BP; 5900 to 5230 yr BP; 3100 to 2800 yr BP; and 2300 to 2000 yr BP. These phases correlated with times of reduced North American Monsoon intensity, suggesting that these communities thrived during drought periods. Variations in non-pollen palynomorphs were synchronic with the lithostratigraphic, geochemical data as well as with the terrestrial changes. During the Meghalayan, compositional shifts in the vegetation and increases in the herbaceous elements indicated human impact, while aquatic taxa, suggested eutrophic and warmer conditions in the lake. By examining the history of this vegetation mosaic, the data on temporal vegetation dynamics during the Holocene offered clues about the response to ongoing global warming and how climate change will likely shape plant communities in western-central Mexico.

Enhancing artificial permafrost table predictions using integrated climate and ground temperature data: A case study from the Qinghai-Xizang highway

The complexities of permafrost changes, driven by climate warming and engineering activities, coupled with challenges in data acquisition, make it crucial and challenging to accurately predict the artificial permafrost table, particularly for subgrades in high-temperature unstable permafrost regions. To address this, this study developed a hybrid machine learning model (RF-LSTM-XGBoost) for permafrost table prediction. By analyzing climate change and ground temperature data from various positions and depths along the subgrade in the Tuotuo River section of the Qinghai-Xizang Highway, the Spearman correlation coefficient method was initially used to determine the important influencing factors. Random Forest (RF), Long Short-Term Memory Neural Network (LSTM), and Extreme Gradient Boosting (XGBoost) models were used to predict the artificial permafrost table, and grid search and cross-validation methods were employed to optimize the hyperparameters of each model. A linear weighted combination method based on the minimum cumulative absolute error was utilized to merge the models, and its performance was compared with the individual RF, LSTM, and XGBoost models. Subsequently, the feature importance of the variables in the machine learning model was analyzed. The results indicated a strong correlation between artificial permafrost table changes and factors such as daily average atmospheric temperature, subgrade surface ground temperature, and subgrade surface ground heat flux during the freezing-thawing cycle. The combined model highlighted daily atmospheric temperature as the most influential predictor, followed by ground heat flux, with the surface ground temperature being less impactful. The combined model demonstrated improved predictive accuracy, with MSE, MAPE, RMSE, MAE, and R2 values of 0.003, 0.052, 0.0085, 0.029, and 0.989, respectively, surpassing those of individual models. This model offers a rapid, accurate, and reliable approach for permafrost table prediction, advancing subgrade stability research in challenging permafrost environments.

Stomatal closure as a driver of minimum leaf conductance declines at high temperature and vapor pressure deficit in Quercus.

Rising global temperatures and vapor pressure deficits (VPD) are increasing plant water demand and becoming major drivers of large-scale plant mortality. Controlling transient leaf water loss after stomatal closure (gmin) is recognized as a key trait determining how long plants survive during soil drought. Yet, substantial uncertainty remains regarding how gmin responds to elevated temperatures and VPD and the underlying mechanisms. We measured gmin in 24 Quercus species from temperate and Mediterranean climates to determine if gmin was sensitive to a coupled temperature and VPD increase. We also explored mechanistic links to phenology, climate, evolutionary history, and leaf anatomy. We found that gmin in all species exhibited a non-linear negative temperature and VPD dependence. At 25°C (VPD = 2.2 kPa), gmin varied from 1.19 to 8.09 mmol m-2 s-1 across species but converged to 0.57 ± 0.06 mmol m-2 s-1 at 45°C (VPD = 6.6 kPa). In a subset of species, the effect of temperature and VPD on gmin was reversible and linked to the degree of stomatal closure, which was greater at 45°C than at 25°C. Our results show that gmin is dependent on temperature and VPD, is highly conserved in Quercus species, and is linked to leaf anatomy and stomatal behavior.

Exploring grapevine canopy management: effects of removing main leaves or lateral shoots before flowering

Over the course of a six-year trial, we investigated the physiological response of the Swiss white cultivar Vitis vinifera Petite Arvine, rich in varietal thiols, to the following canopy removal treatments from the cluster area, i.e. from the shoot base to the sixth leaf of each shoot: A) lateral shoots only, B) lateral shoots + 50 % main leaves, C) lateral shoots + 100 % main leaves or D) main leaves only. All leaf removal (LR) treatments were performed at the pre-flowering stage. Intensive pre-flowering removal of both lateral shoots + 100 % main leaves from the cluster area (C) strongly reduced yield potential (‒47 % on average) and tended to reduce the concentration of 3-mercaptohexanol precursors (Cys-3MH) in the must (‒21 %; p-value < 0.10). The effect of LR on berry set and must composition was modulated by removing fewer main leaves (‒24 % in yield potential and ‒6 % in Cys-3MH concentration). Climate conditions primarily influenced yield and grape composition. Main leaves and lateral shoots played different physiological roles: removal of main leaves only (D) resulted in a larger leaf area (+15 %) due to the development of lateral shoots in the cluster area and a lower yield potential (‒12 %) due to fewer berries per cluster when compared one-to-one with removal of lateral shoots only (A). In the must at harvest, treatment D had higher concentrations of malic acid (+12 %), yeast-assimilable nitrogen (+10 %) and glutathione (+8 %), but there were no significant trends for TSS, pH, Cys-3MH or Folin index. The overall effects of pre-flowering LR on wine composition were negligible in the context of this trial. The study highlighted the different physiological roles of the main leaves and lateral shoots, suggesting that pre-flowering leaf removal should be used cautiously, taking into account the plant’s resilience to environmental conditions. This research is part of a broader project on grapevine canopy management in temperate climates in Switzerland.

Comprehensive Assessment of the Impact of Green Roofs and Walls on Building Energy Performance: A Scientific Review

Sustainability and energy efficiency are now two pivotal goals that society aims towards. Green roofs and facades have gained significant attention in this direction for innovative, sustainable solutions for enhancing building energy performance. With a focus on sustainable urban development and energy-efficient building practices, this study delves into the intricate relationship between these green infrastructure elements and the overall energy dynamics of constructed environments. Furthermore, a range of case studies from diverse geographical locations are presented to provide valuable insights into their practical implications as emerging technologies that contribute to improved insulation, reduced heat transfer, regulating indoor temperatures, and mitigation of urban heat island effects, thus reducing the need for artificial heating and cooling and optimizing overall energy consumption. This comprehensive review serves as a dataset for understanding and highlighting all the research findings of the numerical and experimental investigations invested in the field of greenery systems to encourage their integration, which is crucial for combating climate change and pollution. Previous research is often focused on isolated, short-term, or single-climate analyses of consumption; therefore, by providing an inclusive description of their practical benefits in both temperate and extreme climates, the gap in previous articles is tackled.

DNA-metabarcoding of cyanobacteria and microalgae in chernozem soils of temperate continental climate of the forest-steppe zone of Eurasia under different degrees of agrotechnology intensification.

Chernozem soil is a valuable resource and contains a great diversity of microorganisms that play a global role in the process of soil formation, the species diversity of which has changed over the last five years under the influence of different agrotechnologies. For the first time, under the conditions of the Central Chernozem region, grain and fallow crop rotation, studies using the DNA-metabarcoding method were carried out to study the taxonomic structure of bacteria, fungi, cyanobacteria, and microalgae communities in the arable horizon of typical medium loamy chernozem under winter wheat cultivation. A comparative analysis of the composition of the genotypes showed significant differences in the presented level of mineral nutrition of the soil NPK (60) and NPK (100) compared with the control variant. After processing the 16S and 18S rRNA datasets, a similar trend of decreasing numbers of pro- and eukaryotic species was found from 6296 (control without MF) to 5310 with NPK (60) and to 4643 with NPK (100), respectively. The Chao1 index indicated that the expected diversity within the prokaryotic group was higher in the control without MF at 211, but decreased to 182 and 193 with NPK (60) and NPK (100) fertilizers, respectively. Analysis of the eukaryotic group revealed a 2.6- and 2.9-fold decrease in diversity by class and genus, respectively, depending on the nutritional levels in agrotechnologies, owing to the use of MF. In the prokaryotic community, Alphaproteobacteria microorganisms predominated at an amount of 14.20-14.46%, with Cyanophyceae accounting for 5.2-9.9%. The diversity of eukaryotes was smaller than the number of classes of prokaryotes; the main dominant were Zygnematophyceae 19.5-41%, Chlorophyceae occupied 10.4-15.8%. On the other hand, the doses of fertilizers used contributed to the emergence of dominant species adapted to high doses of mineral nutrients for plants.

Tetranychus urticae Koch Biology on Apple Trees

The two-spotted spider mite (Tetranychus urticae Koch) is a polyphagous arthropod herbivore that consumes over 1,100 plant species from 140 distinct plant families, including those producing harmful chemicals in temperate and tropical climates. It is an important pest in greenhouse and field cultivation, causing damage to various crops such as citrus, apples, grapes, tomatoes, peppers, cucumbers, strawberries, maize, and soy. T. urticae ability to spread over agricultural fields and adapt to environmental stressors is linked to its high propensity for mutation, leading to the highest frequency of pesticide resistance among arthropods. The genetic diversity of T. urticae gathered from apple orchards throughout Poland has been studied to investigate potential correlations between molecular variations and the occurrence of resistance to METI-acaricides. Research has shown that an abundance of leaf pubescence on the abaxial leaf surface adversely affects the development of European red mite, two-spotted spider mite, and carmine spider mite. However, due to variations in leaf sizes, tree canopy, tree ages, and the existence of natural enemies, it is impossible to compare data gathered due to variations in leaf sizes, tree canopy, tree ages, and the presence of natural enemies. Pesticide treatments negatively impact fruit plantations' ecosystems, devastating biodiversity, altering the makeup and structure of arthropod populations, and promoting mass reproduction of phytophage mites by displacing certain species with others. The most extensive research has been done on the common spider mite's development of resistance to avermectin group preparations, with some studies showing resistance to multiple acaricides.