Temperate-zone Animals Research Articles

Transcriptome analyses of nine endocrine tissues identifies organism-wide transcript distribution and structure in the Siberian hamster

Temperate zone animals exhibit seasonal variation in multiple endocrine systems. In most cases, peripheral organs display robust switches in tissue involution and recrudescence in mass. Our understanding of the molecular control of tissue-specific changes in seasonal function remains limited. Central to this problem is the lack of information on the nucleic acid structure, and distribution of transcripts across tissues in seasonal model organisms. Here we report the transcriptome profile of nine endocrine tissues from Siberian hamsters. Luteinizing hormone receptor expression was localized to gonadal tissues and confirmed previous distribution analyses. Assessment of the prolactin receptor reveal relatively high abundance across tissues involved in reproduction, energy, and water homeostasis. Neither melatonin receptor-1a, nor -1b, were found to be expressed in most tissues. Instead, the closely related G-protein coupled receptor Gpr50 was widely expressed in peripheral tissues. Epigenetic enzymes such as DNA methyltransferase 3a, was widely expressed and the predominant DNA methylation enzyme. Quantitative PCR analyses revealed some sex- and tissue-specific differences for prolactin receptor and DNA methyltransferase 3a expression. These data provide significant information on the distribution of transcripts, relative expression levels and nucleic acid sequences that will facilitate molecular studies into the seasonal programs in mammalian physiology.

Hatching phenology is lagging behind an advancing snowmelt pattern in a high-alpine bird

To track peaks in resource abundance, temperate-zone animals use predictive environmental cues to rear their offspring when conditions are most favourable. However, climate change threatens the reliability of such cues when an animal and its resource respond differently to a changing environment. This is especially problematic in alpine environments, where climate warming exceeds the Holarctic trend and may thus lead to rapid asynchrony between peaks in resource abundance and periods of increased resource requirements such as reproductive period of high-alpine specialists. We therefore investigated interannual variation and long-term trends in the breeding phenology of a high-alpine specialist, the white-winged snowfinch, Montifringilla nivalis, using a 20-year dataset from Switzerland. We found that two thirds of broods hatched during snowmelt. Hatching dates positively correlated with April and May precipitation, but changes in mean hatching dates did not coincide with earlier snowmelt in recent years. Our results offer a potential explanation for recently observed population declines already recognisable at lower elevations. We discuss non-adaptive phenotypic plasticity as a potential cause for the asynchrony between changes in snowmelt and hatching dates of snowfinches, but the underlying causes are subject to further research.

A depressed gut makes for a depressed brain via vagal transmission

Many temperate zone animals exhibit seasonal rhythms in physiology and behavior, including seasonal cycles of reproduction, energetics, stress responsiveness, and immune function, among many others. These rhythms are driven by seasonal changes in the duration of pineal melatonin secretion. The neural melatonin target tissues that mediate several of these rhythms have been identified, though the target(s) mediating melatonin’s regulation of glucocorticoid secretion, immune cell numbers, and bacterial killing capacity remain unspecified. The present results indicate that one melatonin target tissue, the paraventricular nucleus of the thalamus (PVT), is necessary for the expression of these seasonal rhythms. Thus, while radiofrequency ablations of the PVT failed to alter testicular and body mass response to short photoperiod exposure, they did block the effect of short day lengths on cortisol secretion and bacterial killing efficacy. These results are consistent with the independent regulation by separate neural circuits of several physiological traits that vary seasonally in mammals.

The paraventricular thalamus serves as a nexus in the regulation of stress and immunity

Many temperate zone animals exhibit seasonal rhythms in physiology and behavior, including seasonal cycles of reproduction, energetics, stress responsiveness, and immune function, among many others. These rhythms are driven by seasonal changes in the duration of pineal melatonin secretion. The neural melatonin target tissues that mediate several of these rhythms have been identified, though the target(s) mediating melatonin’s regulation of glucocorticoid secretion, immune cell numbers, and bacterial killing capacity remain unspecified. The present results indicate that one melatonin target tissue, the paraventricular nucleus of the thalamus (PVT), is necessary for the expression of these seasonal rhythms. Thus, while radiofrequency ablations of the PVT failed to alter testicular and body mass response to short photoperiod exposure, they did block the effect of short day lengths on cortisol secretion and bacterial killing efficacy. These results are consistent with the independent regulation by separate neural circuits of several physiological traits that vary seasonally in mammals.

Photoperiod-induced changes in hypothalamic de novo DNA methyltransferase expression are independent of triiodothyronine in female Siberian hamsters (Phodopus sungorus)

Many temperate zone animals engage in seasonal reproductive physiology and behavior as a strategy to maximise the propagation of the species. The hypothalamus integrates environmental cues and hormonal signalling to optimize the timing of reproduction. Recent work has revealed that epigenetic modifications, such as DNA methylation, vary across seasonal reproductive states. Multiple hormones act in the hypothalamus to permit or inhibit reproductive physiology, and the increase in thyroid hormone triiodothyronine (T3) has been implicated in the initiation of breeding in many species. The objective of this study was to examine the effect of T3 on the photoperiod-dependent regulation of reproductive physiology and hypothalamic DNA methyltransferase enzyme expression in female Siberian hamsters (Phodopus sungorus). We tested the hypothesis that T3 in short days (SD) would stimulate hypothalamic Rfrp3 and de novo DNA methyltransferase (Dnmt) expression in female Siberian hamsters. 10 weeks of SD lengths induced a decrease in body and uterine mass. Hamsters maintained in SD were found to express lower levels of GnRH, Rfrp3, Dnmt3a and Dnmt3b. Two weeks of daily T3 injections did not affect body mass, uterine mass, Gnrh, Rfrp3, Dnmt3a or Dnmt3b expression in neuroendocrine tissues. SD significantly lowered Tshβ mRNA expression and T3 reduced Tshβ in LD hamsters. Our data indicate sex-dependent effects of T3 for the neuroendocrine regulation of seasonal reproduction in hamsters.

Seasonal and Sexual Variation in Metabolism, Thermoregulation, and Hormones in the Big Brown Bat (Eptesicus fuscus)

In response to seasonal variation in energy availability and thermal environment, physiological and endocrine mechanisms have evolved in temperate zone animals. Seasonal changes in hormone activity affect metabolism, body temperature, and reproductive activity. We examined the seasonal regulatory role of hormones on basal metabolic rate (BMR) and regulatory nonshivering thermogenesis (RNST) in 98 female and 17 male adult Eptesicus fuscus (big brown bat). We measured BMR, RNST, and plasma levels of thyroid hormone (T3), leptin, and cortisol in bats captured in maternity colonies in eastern Massachusetts from May to August (from arousal from the hibernation phase to the prehibernation phase). We hypothesized that all three hormones are seasonally primarily metabolic hormones and secondarily thermogenic hormones. In males, only BMR significantly changed seasonally. In females, all five variables significantly changed seasonally. The seasonal pattern of plasma leptin and cortisol levels correlated with the seasonal pattern of BMR, with an initial increase followed by a decrease, suggesting that leptin and cortisol are primarily metabolic hormones. The seasonal pattern of plasma T3 levels generally paralleled the basic seasonal pattern of RNST, with both increasing at the second half of the season, suggesting that T3 is primarily a thermogenic hormone. The observed decrease in plasma leptin levels may be necessary to allow for the observed seasonal decrease in BMR, with the similar cortisol pattern important for leptin regulation. While T3 is needed to maintain BMR, it may play a more critical role in the seasonal regulation of RNST than of BMR.

Vocalizations convey sex, seasonal phenotype, and aggression in a seasonal mammal

Seasonal variation in social behavior is often accompanied by seasonal variation in communication. In mammals, how seasonal environmental cues influence aggressive vocalizations remains underexplored. Photoperiod is the primary cue coordinating seasonal responses in most temperate zone animals, including Siberian hamsters (Phodopus sungorus), a species that undergoes reproductive inhibition and increased aggression in winter. During same-sex aggressive encounters, hamsters emit both broadband calls (BBCs) and ultrasonic vocalizations (USVs) that indicate aggression and the vocalizer's sex, respectively; however, it is not known whether these rodents adjust specific elements of their vocal repertoire to reflect their photoperiod-induced seasonal phenotypes. To address this, we recorded vocalizations emitted during dyadic interactions between male or female pairs of hamsters housed in long or short photoperiods and measured serum testosterone levels. USV emission rate remained stable across photoperiods, but proportional use of USV subtypes varied in novel ways: ‘jump’ USVs were sensitive to seasonal phenotype, but not the vocalizer's sex, whereas ‘plain’ USVs were sensitive only to the sex of the vocalizer. BBC emission rate varied with seasonal phenotype; short-day non-reproductive hamsters produced more BBCs and demonstrated increased aggression compared with reproductive hamsters. Testosterone, however, was not related to vocalization rates. Collectively, these findings demonstrate that changes in the vocal repertoire of Siberian hamsters reflect sex, aggression, and seasonal phenotype, suggesting that both BBCs and USVs are important signals used during same-sex social encounters.

Endocrine rhythms in the brown bear (Ursus arctos): Evidence supporting selection for decreased pineal gland size.

Many temperate zone animals adapt to seasonal changes by altering their physiology. This is mediated in large part by endocrine signals that encode day length and regulate energy balance and metabolism. The objectives of this study were to determine if the daily patterns of two important hormones, melatonin and cortisol, varied with day length in captive brown bears (Ursus arctos) under anesthetized and nonanesthetized conditions during the active (March–October) and hibernation periods. Melatonin concentrations varied with time of day and season in nonanesthetized female bears despite exceedingly low nocturnal concentrations (1–4 pg/mL) in the active season. In contrast, melatonin concentrations during hibernation were 7.5-fold greater than those during the summer in anesthetized male bears. Functional assessment of the pineal gland revealed a slight but significant reduction in melatonin following nocturnal light application during hibernation, but no response to beta-adrenergic stimulation was detected in either season. Examination of pineal size in two bear species bears combined with a phylogenetically corrected analysis of pineal glands in 47 other species revealed a strong relationship to brain size. However, pineal gland size of both bear species deviated significantly from the expected pattern. Robust daily plasma cortisol rhythms were observed during the active season but not during hibernation. Cortisol was potently suppressed following injection with a synthetic glucocorticoid. The results suggest that melatonin and cortisol both retain their ability to reflect seasonal changes in day length in brown bears. The exceptionally small pineal gland in bears may be the result of direct or indirect selection.

Effects of photoperiod and food restriction on the reproductive physiology of female California mice

Many temperate-zone animals use changes in photoperiod to time breeding. Shorter term cues, like food availability, are integrated with photoperiod to adjust reproductive timing under unexpected conditions. Many mice of the genus Peromyscus breed in the summer. California mice (Peromyscus californicus), however, can breed year round, but tend to begin breeding in the winter. Glial cells may be involved in transduction of environmental signals that regulate gonadotrophin releasing hormone I (GnRH) activity. We examined the effects of diet and photoperiod on reproduction in female California mice. Mice placed on either short days (8L:16D) or long days (16L:8D) were food restricted (80% of normal intake) or fed ad libitum. Short day-food restricted mice showed significant regression of the reproductive system. GnRH-immunoreactivity was increased in the tuberal hypothalamus of long day-food restricted mice. This may be associated with the sparing effect long days have when mice are food restricted. The number of GFAP-immunoreactive fibers in proximity to GnRH nerve terminals correlated negatively with uterine size in ad libitum but not food restricted mice, suggesting diet may alter glial regulation of the reproductive axis. There was a trend towards food restriction increasing uterine expression of c-fos mRNA, an estrogen dependent gene. Similar to other seasonally breeding rodents, short days render the reproductive system of female California mice more susceptible to effects of food restriction. This may be vestigial, or it may have evolved to mitigate consequences of unexpectedly poor winter food supplies.

Regulation of heart rate and rumen temperature in red deer: effects of season and food intake

Red deer, Cervus elaphus, like other temperate-zone animals, show a large seasonal fluctuation in energy intake and expenditure. Many seasonal phenotypic adjustments are coordinated by endogenous signals entrained to the photoperiod. The cues determining variation in the resting metabolism of ungulates remain equivocal, however, largely because of the confounding effects of food intake and thus the heat increment of feeding. To distinguish endogenous seasonal and environmental effects on metabolism, we subjected 15 female red deer to two feeding treatments, 80% food restriction and low/high protein content, over two winter seasons in a cross-over design experiment. We used rumen-located transmitters to measure heart rate and rumen temperature, which provided indices of metabolism and core body temperature, respectively. Our mixed model (R²=0.85) indicated a residual seasonal effect on mean daily heart rate that was unexplained by the pellet food treatments, activity, body mass or air temperature. In addition to an apparently endogenous down-regulation of heart rate in winter, the deer further reduced heart rate over about 8 days in response to food restriction. We found a strong correlation between rumen temperature and seasonal or periodic variation in heart rate. An effect of lowered rumen (and hence core body) temperature was enhanced during winter, perhaps owing to peripheral cooling, which is known to accompany bouts of hypometabolism. Our experimental results therefore support the hypothesis that a reduction in body temperature is a physiological mechanism employed even by large mammals, like red deer, to reduce their energy expenditure during periods of negative energy balance.

Neuroanatomical plasticity in the gonadotropin‐releasing hormone system of the ewe: Seasonal variation in glutamatergic and γ‐aminobutyric acidergic afferents

Temperate zone animals time the onset of reproductive events to coincide with specific portions of the sidereal year. Although the neural mechanisms involved remain poorly understood, a marked annual variation in the brain's sensitivity to estradiol negative feedback is thought to mediate many of the changes in neuroendocrine hormone secretion, especially that of the gonadotropin-releasing hormone (GnRH) neurons, via neural afferents. The aim of the present study was to determine whether glutamatergic inputs to GnRH neurons in sheep vary seasonally and to expand our previous observations of seasonal changes in gamma-aminobutyric acid (GABA)-ergic inputs. Brains from adult sheep were collected during the breeding season (N = 8) or the nonbreeding season (anestrus; N = 7). Confocal microscopy and optical sectioning were used to quantify the density of labeled VGLUT2 and VGAT immunoreactivity onto GnRH neurons. The results reveal a significantly greater number of VGLUT2-ir inputs to GnRH dendrites during the breeding season vs. the nonbreeding season but no seasonal changes on GnRH cell somas. The number of VGAT-ir terminals onto GnRH dendrites was reduced in the breeding season compared with the nonbreeding season. GnRH neurons were also found to receive dual-phenotype (VGLUT + VGAT) inputs; these varied with season in a manner similar to VGAT inputs. Morphologically, the numbers of branches of proximal dendrites increased significantly in a subset of GnRH neurons located near the midline. Together these results reveal a dynamic seasonal reorganization of identified inputs onto GnRH neurons and lend additional support to the overall hypothesis that seasonal modulation of GnRH neurons involves glutamatergic and GABAergic neural plasticity.

Cloning of Gonadotropin-Releasing Hormone I Complementary DNAs in Songbirds Facilitates Dissection of Mechanisms Mediating Seasonal Changes in Reproduction

Temperate zone animals exhibit seasonal variation in reproductive physiology. In most cases, seasonal changes in reproductive states are regulated by changes in GnRH1 secretion, rather than synthesis, from the preoptic area (POA)/anterior hypothalamus. An important exception occurs in some songbirds that become photorefractory to the stimulatory effects of long days and show profound decreases in brain GnRH1 protein content. Whether this decline reflects changes in gene expression is unknown because of past failures to measure GNRH1 mRNA levels, due in large part to the absence of available GNRH1 gene sequence in this taxon. Here, we report the first cloning of GNRH1 cDNAs in two songbirds: European starlings and zebra finches. Consistent with the size of the prepro-hormone in other avian and non-avian species, the open-reading frames predict proteins of 91 and 92 amino acids, respectively. Whereas the decapeptide in both species is perfectly conserved with chicken GnRH1, the amino acid identity in the signal peptide and GNRH associated peptide subdomains are significantly less well conserved. At the nucleotide level, the starling and zebra finch coding sequences are approximately 88% identical to each other but only approximately 70% identical to chicken GNRH1. In situ hybridization using radiolabeled cRNA probes demonstrated GNRH1 mRNA expression primarily in the POA, consistent with previous studies on the distribution of the GnRH1-immunoreactive cell bodies. Furthermore, we provide evidence for photoperiod-dependent regulation of GNRH1 mRNA in male starlings. Declines in GNRH1 mRNA levels occur in parallel with testicular involution. Thus, photorefractoriness is associated with decreases in GNRH1 gene expression in the medial POA.

Evolutionary aspects of dietary selection and digestion in the European roe deer (Capreolus capreolus) with special reference to seasonality

The European roe deer (Capreolus capreolus) is the smallest wild ruminant species in the northern hemisphere. Capreolini are represented in the fossil record of Eurasia by an array of forms extending from the Miocene to the Recent. Interestingly, a map of fossil occurrences shows that the historical range is fairly congruent with the recent distribution of Capreolus; roe deer have always been temperate-zone animals of Eurasia (Lister et al. 1998). In contrast to many other large herbivores the roe deer population in Europe increased both in terms of distribution and density during the last 100 years. This emphasises the flexible response of the species to rapidly changing environmental conditions and their potential to adapt to new resources in modern agriculture and forestry. This paper reviews aspects of evolution, dietary selection, digestion and adaptation of the European roe deer to the changing environment. In terms of feeding type, roe deer have been classified as a typical concentrate selector (Hofmann 1989), browsing on highly digestible plant parts. However, this feeding strategy can not be sustained during winter when forage is less available and its digestibility is reduced. Body reserves generally play no major role in roe deer (Holand 1992). Roe deer have developed several mechanisms to adapt to seasonal variations in their environment. It is the only ungulate that is strictly monestric with a genetically fixed diapause. Fawns are born in May when the quality of browse is at an optimum, and twins or even triplets are common. It is concluded that the perfect synchrony between seasonal changes of the resources and the demand due to reproductive investment may be the key function for the success of roe deer in temperate and northern climates.

Kisspeptin Mediates the Photoperiodic Control of Reproduction in Hamsters

The KiSS-1 gene encodes kisspeptin, the endogenous ligand of the G-protein-coupled receptor GPR54. Recent data indicate that the KiSS-1/GPR54 system is critical for the regulation of reproduction and is required for puberty onset. In seasonal breeders, reproduction is tightly controlled by photoperiod (i.e., day length). The Syrian hamster is a seasonal model in which reproductive activity is promoted by long summer days (LD) and inhibited by short winter days (SD). Using in situ hybridization and immunohistochemistry, we show that KiSS-1 is expressed in the arcuate nucleus of LD hamsters. Importantly, the KiSS-1 mRNA level was lower in SD animals but not in SD-refractory animals, which spontaneously reactivated their sexual activity after several months in SD. These changes of expression are not secondary to the photoperiodic variations of gonadal steroids. In contrast, melatonin appears to be necessary for these seasonal changes because pineal-gland ablation prevented the SD-induced downregulation of KiSS-1 expression. Remarkably, a chronic administration of kisspeptin-10 restored the testicular activity of SD hamsters despite persisting photoinhibitory conditions. Overall, these findings are consistent with a role of KiSS-1/GPR54 in the seasonal control of reproduction. We propose that photoperiod, via melatonin, modulates KiSS-1 signaling to drive the reproductive axis.

WHY DO MOST TROPICAL ANIMALS REPRODUCE SEASONALLY? TESTING HYPOTHESES ON AN AUSTRALIAN SNAKE

Most species reproduce seasonally, even in the tropics where activity occurs year-round. Squamate reptiles provide ideal model organisms to clarify the ultimate (adaptive) reasons for the restriction of reproduction to specific times of year. Females of almost all temperate-zone reptile species produce their eggs or offspring in the warmest time of the year, thereby synchronizing embryogenesis with high ambient temperatures. However, although tropical reptiles are freed from this thermal constraint, most do not reproduce year-round. Seasonal reproduction in tropical reptiles might be driven by biotic factors (e.g., peak periods of predation on eggs or hatchlings, or food for hatchlings) or abiotic factors (e.g., seasonal availability of suitably moist incubation conditions). Keelback snakes (Tropidonophis mairii, Colubridae) in tropical Australia reproduce from April to November, but with a major peak in May-June. Our field studies falsify hypotheses that invoke biotic factors as explanations for this pattern: the timing of nesting does not minimize predation on eggs, nor maximize food availability or survival rates for hatchlings. Instead, our data implicate abiotic factors: female keelbacks nest most intensely soon after the cessation of monsoonal rains when soils are moist enough to sustain optimal embryogenesis (wetter nests produce larger hatchlings, that are more likely to survive) but are unlikely to become waterlogged (which is lethal to eggs). Thus, abiotic factors may favor seasonal reproduction in tropical as well as temperate-zone animals.

Effects of Dominance on Vigilance in Avian Social Groups

During winter, many temperate-zone animals must survive harsh environmental conditions of low ambient temperatures, long nights, and unpredictable food supplies. Two evolutionary forces are thought to affect an individual's survivorship during winter: risk of starvation and risk of predation (Lima 1986, McNamara and Houston 1990, Grubb and Pravosudov 1994a, Pravosudov and Grubb 1997). Foraging and vigilance for predators appear to be mutually exclusive forms of behavior that an animal must trade off (Lima 1986). To reduce the risk of starvation, an animal must maximize the rate of foraging and energy intake, but to reduce the risk of predation, it must maximize its vigilance. Social foraging has been thought to benefit all members of a group by allowing each member to increase its foraging efficiency while sharing vigilance with the rest of the group (Elgar 1989). Vigilance in social groups of birds may be directed at predators or dominant group members (Waite 1987a, b; Elgar 1989). Recent studies have suggested that vigilance toward dominant individuals could be directed at both conspecifics and heterospecifics in multispecies groups (Popp 1988, Carrascal and Moreno 1992, Sasvari 1992). Ekman (1987) reported that subordinate Willow Tits (Parus montanus) were more vigilant than dominant individuals. Hogstad (1988a), on the other hand, reported the opposite trend, with dominant Willow Tits being more vigilant than subordinates. The latter report, however, was equivocal because in some instances juvenile females, which always have the lowest dominance rank, had the highest vigilance rates (Hogstad 1988a). However, the absolute rate of vigilance might not be the best measure of benefits for group members. Both studies of Willow Tits examined vigilance of group members within groups but did not provide comparative records for solitary individuals. Because parids seldom (if ever) are solitary in nature, only a manipulative study can provide records for solitary birds under controlled circumstances. Although dominance-related vigilance within the same species occasionally has been studied in controlled experiments (Waite 1987a, b), the effect of heterospecific group members on vigilance has been

The economy of winter: phenotypic plasticity in behavior and brain structure.

Mobile animals must learn the spatial distributions of resources. The cost of foraging increases dramatically for temperate-zone animals during the winter. Two strategies may be used to balance the energetic budget: reducing costs of foraging and reducing need to forage. Both strategies are correlated with changes in brain structure, specifically in the hippocampus, a fore-brain structure used by birds and mammals to map spatial distributions of resources. Small mammals that reduce their need to forage, through hibernation or reduction in body size, show a specific reduction in the structure and size of the hippocampus. The costs of foraging can be also decreased by migration to better foraging conditions or by food-storing, both of which decrease the temporal heterogeneity of food resources. Both of these latter strategies are associated with increased hippocampal structure; for food-storing birds, this increase is a seasonal phenomenon. Thus not only behavior, but also learning ability and even brain structures in adult animals, may be phenotypically plastic in response to the changing demands of the environment.

Age determination in the Red fox (Vulpes vulpes)–an evaluation of technique efficiency as applied to a sample of suburban foxes

A review of age determination techniques that have been applied to the family Canidae is presented. It is shown that the pattern of growth and development of the various species is very similar, the rate of development being greater in the smaller, shorter‐lived species, and vice versa.The occurrence of annuli in the hard tissues of London foxes is demonstrated; these annuli can be used reliably for age determination, despite suggestions that temperate zone animals from weakly continental climates have indistinct annuli. The objective ages determined by cementum annuli are then used to investigate the value of less time‐consuming age determination techniques, particularly in the separation of the young‐of‐the‐year from adults. The results obtained from different studies vary, and these differences are discussed. It is suggested that the speed of development varies slightly in different Red fox populations, and so caution must be exercised before data from one population areapplied to another population.

Ultraviolet radiation thresholds for corneal injury in antarctic and temperate-zone animals

The threshold for corneal damage was determined for a series of antarctic and temperate-zone animals. 1. 1. Keratitis was produced in all animals tested. 2. 2. Susbtantial differences in tolerance levels were found. The thresholds ranged from about 0·3 × 10 6 to 0·6 × 10 6 ergs/cm 2 except for the California ground squirrel, which had a higher threshold of 1·5 · 10 6 ergs/cm 2. 3. 3. The domesticated birds were more sensitive than the antacrtic birds, indicating that adaptation of the cornea to u.v. radiation may be important as protection against snow blindness. 4. 4. Transmission spectra of the cornea showed that the cornea absorbed most of the damaging short wave length u.v. radiation, and that absorption was complete above wave lengths which have the most damaging effect, i.e. near 290 mμ.

Physiological Research in Northern Alaska

Review physiological field research from 1881, citing the scientists and their studies. At the Naval Arctic Research Lab at Barrow there have been projects on blood analysis, nutritional data, energy and fat metabolism, temperature regulation, animal navigation, eye physiology, reproductive physiology of arctic and temperate zone animals, and measurement of time by animals. Techniques, such as the insertion of radio-capsules in bears and foxes, are illustrated and future research needs for data on man and animals discussed.