Temperate Region Of Argentina Research Articles

EL MEJORAMIENTO GENÉTICO DE CEBADILLA CRIOLLA (Bromus catharticus Vahl) EN ARGENTINA: SÍNTESIS DE LOS LOGROS Y AVANCES

The prairie grass (Bromus catharticus Vahl) is the annual/biennial native forage grass of temperate climates of most importance and diffusion in Argentina. Several authors have studied the phenotypic variability in morphophysiological characters in cultivars and native populations of prairie grass. The first commercial cultivars were heterogeneous populations that were considered the best adapted in the temperate region of Argentina. Genetic, agronomic and molecular characterization of the selection base populations and of the germplasm conserved in the active bank of INTA Pergamino, contributed to knowing and conserving the available genetic diversity and, at the same time, provided usable genetic variability in breeding. The most representative cultivars considered in this work were Pergamino Martín Fierro MAG; Fierro Plus INTA, Bar INTA 200, Rosalía INTA and INTA Calvu, in which technological packages associated with the utilization in animal production were developed. Key words: prairie grass, cultivars, germplasm, plant breeding

Photoperiod affects female life history traits in temperate populations of Aedes aegypti from South America

Abstract In many insects, short photoperiods induce females to lay diapausing eggs, which are associated with a reduction in female fecundity, and/or with an increase in their growth rate, allowing for a larger size at adulthood. The effects of short photoperiods may also differ among populations, depending on the different selection pressures imposed by the winter conditions. Thus, this study aimed to experimentally assess the effects of short photoperiods on Aedes aegypti, an invasive mosquito species that also vectors several viral diseases. We compared life history parameters of the females reared from the first larval instar under contrasting photoperiods, in two populations from the temperate region of Argentina, one with a milder winter (BA) and the other with a harsher winter (SB). The results showed a significant increase in the time to oviposition, a trend to a larger size of females (significant only for SB) and higher inhibition of egg hatching (more pronounced in SB), in response to short photoperiods. No differences in fecundity were detected among treatments. The different reaction norms of the populations in female body size and egg hatching response suggest an adaptation to local conditions, which might favour a further expansion of A. aegypti towards colder climates.

Understanding the role of temporal variation of environmental variables in predicting Aedes aegypti oviposition activity in a temperate region of Argentina

Environmental variables related to vegetation and weather are some of the most influential factors that impacting Aedes (Stegomya) aegypti, a mosquito vector of dengue, chikungunya and Zika viruses. In this paper, we aim to develop temporal predictive models for Ae. aegypti oviposition activity utilizing vegetation and meteorological variables as predictors in Córdoba city (Argentina). Eggs were collected using ovitraps placed throughout the city from 2009 to 2012 that were replaced weekly. Temporal generalized linear mixed models were developed with negative binomial distributions of errors that model average number of eggs collected weekly as a function of vegetation and meteorological variables with time lags. The best model included a vegetation index, vapor pressure of water, precipitation and photoperiod. With each unit of increment in vegetation index per week the average number of eggs increased by 1.71 in the third week. Furthermore, each millimeter increase of accumulated rain during 4 weeks was associated with a decrease of 0.668 in the average number of eggs found in the following week. This negative effect of precipitation could occur during abundant rainfalls that fill containers completely, thereby depriving females of oviposition sites and leading them to search for other suitable breeding sites. Furthermore, the average number of eggs increased with the photoperiod at low values of mean vapor pressure; however the average number of eggs decreased at high values of mean vapor pressure, and the positive relationship between the response variable and mean vapor pressure was stronger at low values of photoperiod. Additionally, minimum temperature was associated positively with oviposition activity and that low minimum temperatures could be a limiting factor in Ae. aegypti oviposition activity. Our results emphasize the important role that climatic variables such as temperature, precipitation, and vapor pressure play in Ae. aegypti oviposition activity and how these variables along with vegetation indices can be used to inform predictive temporal models of Ae. aegypti population dynamics that can be used for informing mosquito population control and arbovirus mitigation strategies.

Predation on Eggs of Aedes aegypti (Diptera: Culicidae): Temporal Dynamics and Identification of Potential Predators During the Winter Season in a Temperate Region.

In temperate regions, the populations of Aedes aegypti (L.) remain in the egg stage during the cold season. The ability of these eggs to survive until the next favorable season is affected by several mortality factors, including the action of predators. In the present study, we analyzed the temporal dynamics of the loss of eggs and identified predators of Ae. aegypti eggs along the unfavorable season in a temperate region of Argentina. To this end, eggs were exposed in field conditions in pitfall traps, where walking arthropod taxa were captured during 1-wk periods from early June to early September (Austral winter). The association of arthropod taxa with the loss of eggs was analyzed to identify potential predators. Based on the results obtained, two taxa were chosen to confirm their capacity to consume eggs in a laboratory study. The proportion of lost eggs and the abundance of predators were significantly higher in the winter-spring transition, although results were heterogeneous among traps in all exposure periods. Ants of the genus Strumigenys, isopods of the species Armadillidium vulgare (Isopoda: Armadilloidea), and dermapterans of the species Euborellia annulipes (Dermaptera: Anisolabidae) were positively associated with a high proportion of lost eggs. In laboratory conditions, A. vulgare and E. annulipes consumed the offered eggs, thus confirming their predator capacity. This study represents the first record of predation of Ae. aegypti eggs in temperate South America and the first evidence of dermapterans consuming mosquito eggs.

Low-cost monitoring buoys network tracking biogeochemical changes in lakes and marine environments – a regional case study

Abstract Lakes, rivers, estuaries and ocean waters control many important natural functions at the regional-global level. Hence, integrative and frequent long-term water monitoring is required globally. This paper describes the main features and innovations of a low-cost monitoring buoys network (MBN) deployed in a temperate region of Argentina. The MBN was designed to record extended time series at high-frequency, which is of great value for the scientific community, as well as for decision-makers. In addition, two innovative designs belonging to two versions of moored buoys (i.e. shallow waters and coastal marine waters) were presented. It was shown that the cost of either of two versions of the buoy is low, which can be considered as the main advantage.

Effects of Partial Mixed Rations on Production, Composition and Nutritional Value of Milk in Lactating Dairy Cows in Temperate Region of Argentina

The aim of the study was to determine the effect of supplementing an oat winter pasture with a total mixed ration (TMR) on lactation performance of dairy cows. Nine multiparous Holstein cows were used in a 3 × 3 Latin square design with three TMR: pasture ratios at 79:21 (T1), 58:42 (T2) and 33:67 (T3) on a dry matter (DM) basis. The response to the 100% TMR diet (TMR-100) was tested using an extra period of 14 days inmediately after finishing the Latin square schedule using the nine experimental cows. Total DM intake resulted higher in TMR-100 (28.2 (kg/cow·day) and decreased by 2.64 kg/cow·day in T1, 5.02 kg in T2 and 6.68 kg in T3. Yields of milk (31.2 kg/cow·day) and fat corrected milk (26.8 kg/cow·day) was similar between T1, T2 and T3. Milk yield resulted higher in TMR-100 (32.1 kg/cow·day) compared to T2 (30.7 kg/cow·day). Milk fat concentration (g/100 g) was higher in T2 (3.13) and T3 (3.20) compared to T1 (2.96) and TMR-100 (2.85) and milk fat yield did not differ. Milk protein content resulted higher in TMR-100 and T2 averaging 3.37 g/100 g compared to T1 (3.32 g/100 g). The lowest milk protein concentration was observed in T3 (3.29 g/100 g) that resulted only different to T2. Milk protein yield did not differ between T1, T2 and T3 averaging 1.03 kg/cow·day. A lower protein yield was detected in T2 (1.03) and T3 (1.00) when compared to TMR-100 (1.07). Efficiency of milk production (kg milk/kg DM intake) resulted higher in T3 (1.42) compared to T1 (1.25). Treatments that included pasture yielded a higher efficiency of milk production when compared to TMR-100 (1.13). Plasma urea concentration resulted lower in TMR-100 (33.8 mg/dl) with no significant differences for this parameter between the other treatments. Glucose plasma levels did not differ between T1, T2 and T3 but plasma non-esterified fatty acids (NEFA) gradually increased as the proportion of pasture was enhanced according to the lower energy intake. Plasma insulin levels were higher in TMR-100 and T2 whereas concentration of somatomedins (IGF-1) remained unaffected. Grouth hormone (GH) levels and the GH/insulin ratio were highly variable and not affected by treatments. Concentration of vaccenic and conjugated linoleic acids gradually increased with the inclusion of pasture in the diet. Supplementing a winter oat pasture with TMR may be a suitable strategy to maintain milk production with a high conversion efficiency but part of the produced milk could be sustained at the expense of endogenous energy mobilization. The conditions of replacing pasture for TMR in high yielding dairy cows should be defined taking into account the depressing effect of pasture on total DM and energy intake when fresh forage is included in high proportion in the diet.

Records of epigeal spiders in Bahia Blanca in the temperate region of Argentina

Ecological surveys of diversity and seasonal patterns of spiders in relation with cadavers have rarely been conducted, despite the high potential species diversity and abundance of spiders. The composition, abundance, guilds and seasonality of the spiders of Bahía Blanca, in the temperate region of Argentina were analysed. The study was performed in a semi-rural area between 2010 and 2011, using pitfall traps that were checked daily during 168 ± 7 days in winter, 38 days in spring, 30 days in summer, and 71 days in autumn. A total of 972 spiders were recorded belonging to 22 families and 65 species/morphospecies. The most abundant families were: Lycosidae, Zodariidae, Thomisidae, Theridiidae and Salticidae. The guild with more specimens was represented by ground hunters followed by specialists and ambush hunters, and the guild with the greatest number of species corresponded also to the ground hunters. Considering all specimens, adults prevailed with respect to juveniles, and males were more abundant than females. The more abundant species were Leprolochus birabeni, Steatoda sp. 1, Thomisidae sp. 33, Thomisidae sp. 32, Ostearius melanopygius and Metaltella simoni.

Presence of <I>Dalbulus maidis</I> (Hemiptera: Cicadellidae) and of <I>Spiroplasma kunkelii</I> in the Temperate Region of Argentina

"Corn stunt" is one of the main corn (Zea mays L.) diseases in the Americas and Dalbulus maidis (DeLong & Wolcott) is the key vector of the pathogen Spiroplasma kunkelii Whitcomb. In Argentina, the corn-producing area is in the temperate region, where vector and pathogen prevalence levels are unknown. In this study, the prevalence and distribution of D. maidis and S. kunkelii in the temperate region of Argentina and D. maidis overwintering ability in this region were determined. Surveys were conducted in 2005-2006 and 2006-2007 seasons to determine D. maidis and S. kunkelii presence, and in winter 2006 to determine the vector overwintering ability. The highest S. kunkelii prevalence and incidence levels were found in the transition area from the temperate to the subtropical region, related to the highest D. maidis prevalence and insects sampled per location. D. maidis adults were found in volunteer corn plants and spontaneous vegetation in autumn and winter months, which were inoculative for the pathogen S. kunkelii. This overwintering ability was related to detection of D. maidis insects in corn crops at early growth stages in the following growing season. This work emphasizes that corn stunt disease is present in the temperate region of Argentina, and this highlights the need to develop proper agronomic practices like monitoring insect vector populations and controlling voluntary plants. This study also indicates that further research is needed to understand the potential yield reduction caused by this pathogen on symptomless plants and population dynamics of the insect vector.

Heterosis × environment interaction in maize: What drives heterosis for grain yield?

Variation in mean heterosis over a range of environments is expected when maize hybrids and inbred lines respond differently to environmental stimuli. However, the magnitude and nature of the heterosis × environment interaction (H × E) has not been adequately described. The objectives of this work were to determine (i) the effects of environmental variability on the expression of heterosis for plant grain yield (PGY) and related ecophysiological traits and (ii) to what extent H × E is of general importance for the expression of heterosis for these traits. Field experiments included a set of six inbred lines and twelve derived hybrids grown in 14 environments (year × nitrogen × water regime combinations) in the temperate region of Argentina. Main physiological and quantitative determinants of PGY were measured and mid parent heterosis (MPH) computed for each trait. Genotype × environment interaction was investigated using the joint regression analysis. For PGY, hybrids had a significant but moderate association between sensitivity to the environment and mean genotype value, whereas inbred lines did not show association. For harvest index (HI) hybrids showed greater mean values than inbreds, however, regression coefficients of both genotype groups tended to overlap slightly. A decrease in environmental quality led to a decline in the expression of heterosis for PGY but not for HI. A bilinear with plateau model adequately described the association between heterosis for PGY and environmental quality, because a threshold value was detected beyond which further increases in environment mean did not translate into higher heterosis for PGY. A similar response pattern was found between PGY MPH and biomass at physiological maturity (Biomass PM) MPH. Despite the greater heterosis for Biomass PM, further increases in PGY MPH could not be realized above a threshold value of 115 g pl −1 for Biomass PM MPH. HI MPH was the major factor that set a limit to PGY MPH under favorable environments.

Ecological differences between two sympatric species of armadillos (Xenarthra, Mammalia) in a temperate region of Argentina

Morpho-physiological specialization related to foraging can act as constraints on behaviour and ecological patterns of abundance and distribution. We tested this prediction in two species (weeping or screaming hairy armadillos Chaetophractus vellerosus Gray, 1865 and mulitas Dasypus hybridus Desmarest, 1804) that represent the two subfamilies of armadillos (Dasypodidae, Xenarthra): Euphractinae and Dasypodinae. The first subfamily possesses a well-developed masticatory apparatus that is considered primitive within the Xenarthra, while Dasypodinae show reductions in various aspects of cranial morphology. We sampled signs (burrows and foraging holes) and captured both species on 34 farms randomly selected in the north-eastern Pampas region of Argentina. We analyzed several niche dimensions. The two species showed significant differences in habitat use, seasonal patterns and diet. Weeping armadillos were generalists in their diet and active throughout the year. They were associated with calcareous-sand soil, as expected for a burrowing animal of the deserts. They tolerated a wide range of environmental conditions. We found that mulitas are myrmecophagous, and that they demonstrated a reduction in body temperature and activity during the cold season. As expected from their geographical distribution, mulitas used mainly vegetation with high cover and were associated with humic soils. Niche segregation between the two species of armadillos appeared to originate from different degrees of dietary specialization.

Variability Among Four Laboratories of the Filter Bag Technique to Determine Acid and Neutral Detergent Fiber Contents in Ruminant Feeds from Argentina

The objective of this work was to evaluate the filter bag technique (FBT) in an ANKOM 200 fiber analyzer to assess its accuracy for the determination of acid and neutral detergent fiber (ADF and NDF, respectively) contents in common ruminant feeds from the temperate region of Argentina. The evaluation was done in a ring test of 4 laboratories. The 3 Argentine laboratories were from the College of Agronomy, University of Buenos Aires, the College of Agricultural Sciences of the Catholic University of Argentina, and the Balcarce Agricultural Experiment Station of the National Institute of Agricultural Technology (INTA). The fourth laboratory was from the Department of Animal Science of Cornell University, Ithaca, NY. The feeds utilized were soybean meal expeller, whole sunflower seed, corn grain, herbages of alfalfa, ryegrass, tall wheatgrass, weeping lovegrass, guinea and barley grasses, and silages of corn and of a natural grassland. Results indicated that values obtained in the 4 laboratories were consistent regardless of the laboratory where the analyses were performed. It was concluded that the FBT in the ANKOM instrument gave satisfactory results when used for the determination of both ADF and NDF across samples having a wide range of fiber contents, and that the among-laboratories variability of this methodology was low.

Evaluation of Two Procedures to Determine Acid and Neutral Detergent Fibers in Ruminant Feeds of the Temperate Region of Argentina

The objective of this work was to compare the traditional Van Soest's procedure to analyze neutral detergent fibers (NDF) and acid detergent fibers (ADF) using filtering crucibles (VS) with a semiautomatic method which uses filter bags in an ANKOM Technology Corp. instrument (ANK). Nine ruminant feeds widely used in the temperate region of Argentina were analyzed: soybean meal expeller, alfalfa, pasture silage, ryegrass, corn silage, weeping lovegrass, tall wheatgrass, guinea grass, and barley grass. Four runs were done for each technique and feed. The comparison of ADF and NDF means by the means test for paired samples showed no significant differences between techniques (alpha = 0.01). The variability among runs was greater with the VS method than the ANK, both for NDF, standard deviation (SD) = 0.71 vs 0.39, and for ADF, SD = 0.83 vs 0.56, but the differences were not significant. The linear regressions were VS = 1.43 + 0.95 ANK; R2 = 0.99, and VS = 0.53 + 0.98 ANK; R2 = 0.99 for NDF and ADF, respectively, which indicated a strong linear relationship among the results of both procedures. It was concluded that the ANK procedure gave results comparable to those of the VS method when ruminant feeds from the temperate region of Argentina were analyzed.

Intercepted Radiation at Flowering and Kernel Number in Maize

When water and nutrients are not limiting growth, kernel number per plant (KNP) can be calculated for maize (Zea mays L.) using linear models based on intercepted photosynthetically active radiation per plant (IPARP). These models do not include the concept of a threshold IPARP for KNP. Our objective was to study the response of KNP to IPARP in order to improve current models. Published information from several field experiments, performed with two maize hybrids in the temperate region of Argentina, was used for the analysis. Both linear and curvilinear (inverse linear and exponential) models based on IPARP explained more than 75% of the variability in KNP and kernel number per apical ear (KNA). Curvilinear models gave a better fit at low (<0.5 MJ plant−1 d−1) and intermediate (1.3‐2 MJ plant−1 d−1) values of IPARP than a simple two‐line linear model. Models allowed the estimation of maximum KNA and maximum KNP, and the minimum IPARP to reach them, but only curvilinear models allowed the estimation of a threshold IPARP (0.31‐0.37 MJ plant−1 d−1) to avoid plant barrenness. The inverse linear model gave a better prediction of maximum kernel number than the other two models. The exponential model allowed a better estimation of the initial value (2.16 MJ plant−1 d−1) for expressing prolificacy (ears plant−1) > 1. The relationship between KNP and IPARP did not improve when IPARP was expressed on a thermal time basis (photothermal quotient), probably because air temperature did not vary much among the situations explored in this work.