Abstract Latino adults in the U.S. have low rates of colorectal cancer (CRC) screening. Using at-home fecal immunochemical tests (FIT) can improve screening rates. This study builds upon three-minute instructional animated videos originally created by the Colorectal Cancer Prevention Network that our team previously translated into Spanish. In the current study, we employed a user-centered approach, known as learner verification and revision (LV&R), to elicit patients’ input on the videos. These videos will be used as part of a text-based intervention aimed at increasing FIT use among Latino adults aged 45-54. This study was part of a larger trial, Community Partnership for Telehealth Solutions to Counter Misinformation and Achieve Equity (PRIME), which aims to test an intervention that integrates the videos with social-needs navigation to boost CRC screening within Latino communities. LV&R integrates theories of health communication and qualitative research to tailor interventions to the needs of target populations. To solicit feedback on the two videos (one on FIT, one on colonoscopy), we interviewed 24 patients (46% male) from a large urban community clinic in Los Angeles. Patients were age-eligible for CRC screening, had been mailed a FIT, texted a link to the videos, and spoke English or Spanish (54% preferred Spanish). Interviews asked about acceptability, comprehension, actionability, and cultural relevance of the videos among our target audience. Interviews were recorded, transcribed, and summarized using rapid qualitative analysis techniques. Interviews revealed positive perceptions of both videos' accessibility, content, and appeal. Participants appreciated the videos’ straightforward language and clear instructions, which were found to be culturally and linguistically acceptable across English and Spanish-speakers. The videos were viewed as informative and persuasive, motivating participants to consider or complete CRC screening and share videos with others. The videos aided in dispelling misinformation related to screening with FIT and/or colonoscopy, particularly for those who had never screened for CRC. Participants liked the length of the videos along with use of appropriate humor to convey complex steps in the screening process. Participants suggested minor improvements such as adding language related to bowel prep, screening guidelines, communication of results, and reassuring language to help with fears to enhance the videos' effectiveness. Overall, these findings underscore the importance of culturally responsive, informative, and engaging materials in promoting CRC screening awareness and participation. Latino patients reported high acceptability, persuasiveness, helpfulness, and cultural appropriateness of animated videos sent via text to educate patients about FIT and colonoscopy. Suggestions for improvement centered on providing more facts about CRC, emphasizing that both men and women are at risk, adding reassuring language to encourage individuals to overcome any fears, and providing more resources for further information. Citation Format: Jennifer S. Rivelli, Jennifer L. Schneider, Blake W. Locher, Katherine A. Vaughn, Meagan C. Shaw, Gloria D. Coronado, Esmeralda Ruiz, Jamie H. Thompson, Anne Escaron. Improving FIT and colonoscopy animated videos for colorectal cancer screening: An application of learner verification and revision [abstract]. In: Proceedings of the 17th AACR Conference on the Science of Cancer Health Disparities in Racial/Ethnic Minorities and the Medically Underserved; 2024 Sep 21-24; Los Angeles, CA. Philadelphia (PA): AACR; Cancer Epidemiol Biomarkers Prev 2024;33(9 Suppl):Abstract nr A150.