High-resolution Imaging Techniques Research Articles

Neuroethology: A Review of Studies on Combat Behavior in Mice

Optogenetics has revolutionized neuroscience, enabling precise control and monitoring of neural circuits. This study focuses on the neural mechanisms underlying combat behaviors in mice, particularly the roles of the ventromedial hypothalamus (VMH) in freezing, jumping escape, and defensive attack behaviors. The research employed a combination of optogenetic tools, behavioral assays, and high-resolution imaging techniques. Genetically modified mice expressing channelrhodopsin-2 (ChR2) and halorhodopsin (NpHR) were used. Viral vectors were injected into specific brain regions, and optogenetic stimulation was performed to activate or inhibit neuronal populations. Behavioral responses were documented and analyzed using specialized software, and brain tissues were prepared for histological examination. Activation of VMH neurons increased jumping escape behavior, while inhibition reduced defensive attack behaviors, highlighting the VMH's critical role in these responses. High-resolution video analysis provided detailed metrics on behavior, and histological examination confirmed precise targeting and expression of optogenetic proteins in the brain. The results demonstrated the direct influence of specific neural circuits on combat behaviors. The study elucidated the roles of VMH and associated neural circuits in mediating combat behaviors in mice. Optogenetic techniques provided precise control over neuronal activity, revealing critical insights into the neural mechanisms underlying these behaviors. The findings support the VMH's central role in regulating instinctual defensive actions and offer potential applications for developing therapeutic strategies for anxiety and pain disorders.

OCTA: Essential or Gimmick?

This commentary article delves into the transformative role of optical coherence tomography angiography (OCTA) in diagnosing and managing a wide array of eye conditions, including diabetic retinopathy, age-related macular degeneration, retinal vein occlusions, and white dot syndromes. Developed in 2005, OCTA has emerged as a non-invasive, high-resolution imaging technique that offers advantages over traditional fluorescein angiography (FA), providing quicker and safer monitoring of ocular conditions with similar diagnostic accuracy. In diabetic retinopathy, OCTA has been instrumental in early identification of retinal changes, offering quantifiable metrics including perfused capillary density (PCD) for assessing vascular alterations. For age-related macular degeneration (AMD), OCTA has deepened our understanding of non-exudative neovascular AMD, allowing for more effective monitoring and potential earlier initiation of treatment. In cases of retinal vein occlusions, OCTA can reveal specific microvascular features and allow for depth-resolved measurements of the foveal avascular zone, providing significant prognostic implications. OCTA has also been invaluable in studying rare white dot syndromes, enabling nuanced differentiation between conditions that often present similarly. Emerging research also suggests that OCTA can have potential utility in neurodegenerative diseases like Alzheimer's, where retinal vascular patterns could offer diagnostic insights. While OCTA is revolutionizing ophthalmic care, further clinical trials and standardization are needed for its broader adoption into clinical practice.

Enhanced Characterization of Fractured Zones in Bedrock Using Hydraulic Tomography through Joint Inversion of Hydraulic Head and Flux Data

Hydraulic tomography (HT) is a promising technique for high-resolution imaging of subsurface heterogeneity, which addresses the limitations of traditional methods, such as borehole drilling and geophysical surveys. This study focuses on the application of HT to detect and characterize fractured zones in bedrock and addresses the gap in the understanding of the role of distributed flux data in the joint inversion of hydraulic head and flux data. By conducting synthetic injection tests and using sequential successive linear estimators for inversion, the study explores the effectiveness of combining limited head data with distributed temperature sensing (A-DTS)-derived flux data. The findings highlight the fact that integrating flux data significantly enhances the accuracy of identifying fracture permeability characteristics, even when head data is sparse. This approach not only improves the resolution of hydraulic conductivity fields but also offers a cost-effective strategy for practical field applications. The results underscore the potential of HT to enhance our understanding of groundwater flow and contaminant transport in fractured media, which has important implications for carbon capture, enhanced geothermal systems, and radioactive waste disposal.

Efficacy of high-resolution vessel wall MRI in the postoperative assessment of intracranial aneurysms following flow diversion treatment.

Certain patients must undergo frequent postoperative digital subtraction angiography (DSA) after flow diversion (FD) therapy. No imaging modality with an efficacy comparable to that of DSA has been established. This study was conducted to determine the efficacy of contrast-enhanced delay alternating with nutation for tailored excitation (DANTE) T1-sampling perfection with application-optimized contrasts by using different flip angle evolution (SPACE), a high-resolution vessel wall magnetic resonance imaging technique for evaluating the occlusion status of intracranial aneurysms after FD treatment, with DSA serving as the reference standard. This retrospective study included 23 patients with 26 aneurysms who had undergone FD treatment between April 2016 and May 2022. Contrast-enhanced DANTE T1-SPACE and DSA were performed as postoperative follow-up imaging studies at 45 time points, both in the same period. The agreement rates for aneurysm occlusion status in the 45 imaging studies were examined. Contrast-enhanced DANTE T1-SPACE had a sensitivity and specificity of 96.3% (26/27) and 83.3% (15/18), respectively, for detecting aneurysm remnants. Overall, 91.1% (41/45) of findings detected on contrast-enhanced DANTE T1-SPACE were consistent with those on DSA. The findings detected on contrast-enhanced DANTE T1-SPACE were completely consistent with those of intraaneurysmal residual blood flow identified on DSA or high-resolution cone-beam computed tomography images in 74.1% (20/27) of the examinations that showed incomplete occlusion on DSA. Furthermore, parent artery status after FD treatment on contrast-enhanced DANTE T1-SPACE was consistent with that observed on DSA in 97.8% (44/45) of examinations. Contrast-enhanced DANTE T1-SPACE is a very useful option in the follow-up of aneurysms after FD treatment.

Late-stage microstructures in Chang’E-5 basalt and implications for the evolution of lunar ferrobasalt

Abstract This study investigates silicate liquid immiscibility (SLI) microstructures in the Chang’E-5 (CE-5) lunar ferrobasalt sample, the youngest recovered mare basalt (ca. ~2.0 Ga). Employing advanced high-resolution imaging techniques and chemical analysis, we examined a subophitic fragment, revealing two distinct types of microstructures indicative of multi-stage SLI events. The first type is observed in the mesostasis pockets and exhibits both “sieve” and “maze” textures, where the Si-K rich glassy phases are interconnected with Fe-rich minerals, e.g., fayalite. This type of microstructure, similar to previous observations in Apollo and Luna samples, is the product of a stable SLI event. The second type is characterized by K-free but high-Si melt inclusions occurring as emulsions in the rims of plagioclase. The entrapment of these emulsions followed a metastable SLI event, with the Fe-rich liquids serving as precursors to subsequent stable SLI processes. Additionally, the Fe-rich droplets within the emulsions underwent coarsening via Ostwald ripening, a phenomenon in which smaller particles in solution dissolve and deposit on larger particles. Our simulation of this coarsening process suggests a duration of at least 15-32 days for the SLI processes, alongside a slow cooling rate (< 0.3 °C/hour) of the late-stage CE-5 lava. We propose that metastable SLI may have influenced the effusive signature of the CE-5 lava flow during its late-stage evolution. The metastable SLI process can potentially lead to the formation of various phases during the late-stage evolution of lunar ferrobasaltic magmas, thereby contributing to the diversity of lunar rock types.

Initial findings using high-resolution magnetic resonance imaging for visualisation of the sural nerve and surrounding anatomy in healthy volunteers at 7 Tesla.

Histopathological diagnosis is the gold standard in many acquired inflammatory, infiltrative and amyloid based peripheral nerve diseases and a sensory nerve biopsy of sural or superficial peroneal nerve is favoured where a biopsy is deemed necessary. The ability to determine nerve pathology by high-resolution imaging techniques resolving anatomy and imaging characteristics might improve diagnosis and obviate the need for biopsy in some. The sural nerve is anatomically variable and occasionally adjacent vessels can be sent for analysis in error. Knowing the exact position and relationships of the nerve prior to surgery could be clinically useful and thus reliably resolving nerve position has some utility. 7T images of eight healthy volunteers' (HV) right ankle were acquired in a pilot study using a double-echo in steady-state sequence for high-resolution anatomy images. Magnetic Transfer Ratio images were acquired of the same area. Systematic scoring of the sural, tibial and deep peroneal nerve around the surgical landmark 7 cm from the lateral malleolus was performed (number of fascicles, area in voxels and mm2, diameter and location relative to nearby vessels and muscles). The sural and tibial nerves were visualised in the high-resolution double-echo in steady-state (DESS) image in all HV. The deep peroneal nerve was not always visualised at level of interest. The MTR values were tightly grouped except in the sural nerve where the nerve was not visualised in two HV. The sural nerve location was found to be variable (e.g., lateral or medial to, or crossing behind, or found positioned directly posterior to the saphenous vein). High-resolution high-field images have excellent visualisation of the sural nerve and would give surgeons prior knowledge of the position before surgery. Basic imaging characteristics of the sural nerve can be acquired, but more detailed imaging characteristics are not easily evaluable in the very small sural and further developments and specific studies are required for any diagnostic utility at 7T.

Elucidating bacterial coaggregation through a physicochemical and imaging surface characterization

Bacterial coaggregation is a highly specific type of cell-cell interaction, well-documented among oral bacteria, and involves specific characteristics of the cell surface of the coaggregating strains. However, the understanding of the mechanisms promoting coaggregation in aquatic systems remains limited. This gap is critical to address, given the broad implications of coaggregation for multispecies biofilm formation, water quality, the performance of engineered systems, and diverse biotechnological applications. Therefore, this study aims to comprehensively characterize the cell surface of the coaggregating strain Delftia acidovorans 005P, isolated from drinking water, alongside a non-coaggregating strain, D. acidovorans 009P. By analyzing two strains of the same species, we aim to identify the factors contributing to the coaggregation ability of strain 005P. To achieve this, we employed a combination of physicochemical characterization, Fourier-transform infrared spectroscopy (FTIR), and advancing imaging techniques [transmission electron microscopy and cryo-electron tomography (cryo-ET)]. The coaggregating strain (005P) exhibited higher surface hydrophobicity, negative surface charge, and cell surface and co-adhesion energies than the non-coaggregating strain (009P). The chemical characterization of bacterial surfaces through FTIR revealed subtle differences, particularly in spectral regions linked to carbohydrates and phosphodiesters/amide III of proteins (860–930 cm−1 and 1212–1240 cm−1, respectively). Cryo-ET highlighted significant differences in pili structures between the strains, such as variations in length, frequency, and arrangement. The pili in the 005P strain, identified as pili-like adhesins, serve as key mediators of coaggregation. By integrating physicochemical analyses and high-resolution imaging techniques, this study conclusively links the coaggregation ability of D. acidovorans 005P to its unique pili characteristics, emphasizing their crucial role in microbial coaggregation in aquatic environments.

High-resolution live cell imaging to define ultrastructural and dynamic features of the halotolerant yeast Debaryomyces hansenii.

Although some budding yeasts have proved tractable and intensely studied models, others are more recalcitrant. Debaryomyces hansenii, an important yeast species in food and biotechnological industries with curious physiological characteristics, has proved difficult to manipulate genetically and remains poorly defined. To remedy this, we have combined live cell fluorescent dyes with high-resolution imaging techniques to define the sub-cellular features of D. hansenii, such as the mitochondria, nuclei, vacuoles and the cell wall. Using these tools, we define biological processes like the cell cycle, organelle inheritance and various membrane trafficking pathways of D. hansenii for the first time. Beyond this, reagents designed to study Saccharomyces cerevisiae proteins were used to access proteomic information about D. hansenii. Finally, we optimised the use of label-free holotomography to image yeast, defining the physical parameters and visualising sub-cellular features like membranes and vacuoles. Not only does this work shed light on D. hansenii but this combinatorial approach serves as a template for how other cell biological systems, which are not amenable to standard genetic procedures, can be studied.

Diagnostic value of high-resolution vessel wall imaging technique in intracranial arterial stenosis and occlusion: a comparative analysis with digital subtraction angiography

Objective To analyze the diagnostic value of HR-VWI in intracranial arterial stenosis and occlusion and compare it with DSA. Methods A retrospective analysis of clinical data of 59 patients with intracranial arterial stenosis in our hospital was conducted to compare the diagnostic results of the two methods for different degrees of intracranial stenosis and various morphological plaques. Results The diagnosis of stenosis and occlusion by both methods showed no significant difference (p > 0.05). Comparison of plaque morphology detected by HR-VWI with pathological examination results showed no significant difference (p > 0.05); however, there was a significant difference between plaque morphology detected by DSA and pathological examination results (p < 0.05). Additionally, there was a significant difference between plaque morphology detected by HR-VWI and DSA (p < 0.05). Conclusion HR-VWI technique is comparable to DSA technique in diagnosing intracranial arterial stenosis and occlusion, but it is superior to DSA in plaque morphology diagnosis.

Exploring the Capability of Cu-MoS2 Catalysts for Use in Electrocatalytic Overall Water Splitting.

Herein, we prepare MoS2 and Cu-MoS2 catalysts using the solvothermal method, a widely accepted technique for electrocatalytic overall water-splitting applications. TEM and SEM images, standard tools in materials science, provide a clear view of the morphology of Cu-MoS2. HRTEM analysis, a high-resolution imaging technique, confirms the lattice spacing, lattice plane, and crystal structure of Cu-MoS2. HAADF and corresponding color mapping and advanced imaging techniques reveal the existence of the Cu-doping, Mo, and S elements in Cu-MoS2. Notably, Cu plays a crucial role in improving the hydrogen evolution reaction (HER) and oxygen evolution reaction (OER) of the Cu-MoS2 catalyst as compared with the MoS2 catalyst. In addition, the Cu-MoS2 catalyst demonstrates significantly lower overpotential (167.7 mV and 290 mV) and Tafel slopes (121.5 mV dec-1 and 101.5 mV dec-1), standing at -10 mA cm-2 and 10 mA cm-2 for HER and OER, respectively, compared to the MoS2 catalyst. Additionally, the Cu-MoS2 catalyst displays outstanding stability for 12 h at -10 mA cm-2 of HER and 12 h at 10 mA cm-2 of OER using chronopotentiaometry. Interestingly, the Cu-MoS2‖Cu-MoS2 cell displays a lower cell potential of 1.69 V compared with the MoS2‖MoS2 cell of 1.81 V during overall water splitting. Moreover, the Cu-MoS2‖Cu-MoS2 cell shows excellent stability when using chronopotentiaometry for 18 h at 10 mA cm-2.

EDTP enhances and protects the fluorescent signal of GFP in cleared and expanded tissues

Advanced 3D high-resolution imaging techniques are essential for investigating biological challenges, such as neural circuit analysis and tumor microenvironment in intact tissues. However, the fluorescence signal emitted by endogenous fluorescent proteins in cleared or expanded biological samples gradually diminishes with repeated irradiation and prolonged imaging, compromising its ability to accurately depict the underlying scientific problem. We have developed a strategy to preserve fluorescence in cleared and expanded tissue samples during prolonged high-resolution three-dimensional imaging. We evaluated various compounds at different concentrations to determine their ability to enhance fluorescence intensity and resistance to photobleaching while maintaining the structural integrity of the tissue. Specifically, we investigated the impact of EDTP utilization on GFP, as it has been observed to significantly improve fluorescence intensity, resistance to photobleaching, and maintain fluorescence during extended room temperature storage. This breakthrough will facilitate extended hydrophilic and hydrogel-based clearing and expansion methods for achieving long-term high-resolution 3D imaging of cleared biological tissues by effectively safeguarding fluorescent proteins within the tissue.

Three short-period Earth-sized planets around M dwarfs discovered by TESS: TOI-5720 b, TOI-6008 b, and TOI-6086 b

One of the main goals of the NASA Transiting Exoplanet Survey Satellite (TESS) mission is the discovery of Earth-like planets around nearby M-dwarf stars. We present the discovery and validation of three new short-period Earth-sized planets orbiting nearby M dwarfs: TOI-5720 b, TOI-6008 b, and TOI-6086 b. We combined TESS data, ground-based multicolor light curves, ground-based optical and near-infrared spectroscopy, and Subaru/IRD radial velocity data to validate the planetary candidates and constrain the physical parameters of the systems. In addition, we used archival images, high-resolution imaging, and statistical validation techniques to support the planetary validation. TOI-5720 b is an Earth-sized planet with a radius of Rp = 1.09 ± 0.07 R⊕. It orbits a nearby (36 pc) M 2.5 host with an orbital period of P = 1.4344555 ± 0.0000036 days. It has an equilibrium temperature of Teq = 708 ± 19 K (assuming a null albedo) and an incident flux of Sp = 41.7 ± 4.5 S⊕. TOI-6008 b is a short-period planet of P = 0.8574347 ± 0.0000424 day. It has a radius of Rp = 1.03 ± 0.05 R⊕, an equilibrium temperature of Teq = 707 ± 19 K, and an incident flux of Sp = 41.5 ± 4.5 S⊕. The host star (TOI-6008) is a nearby (23 pc) M 5 with an effective temperature of Teff = 3075 ± 75 K. Based on the radial velocity measurements collected with Subaru/IRD, we set a 3σ upper limit of Mp &lt; 4 M⊕, thus ruling out a star or brown dwarf as the transiting companion. TOI-6086 b orbits its nearby (32 pc) M 3 host star (Teff = 3200 ± 75 K) every 1.3888725 ± 0.0000827 days and has a radius of Rp = 1.18 ± 0.07 R⊕, an equilibrium temperature of Teq = 634 ± 16 K, and an incident flux of Sp = 26.8 ± 2.7 S⊕. Additional high-precision radial velocity measurements are needed to derive the planetary masses and bulk densities and to search for additional planets in the systems. Moreover, short-period Earth-sized planets orbiting around nearby M dwarfs are suitable targets for an atmospheric characterization with the James Webb Space Telescope through transmission and emission spectroscopy and phase-curve photometry.

Dual-beam X-ray nano-holotomography.

Nanotomography with hard X-rays is a widely used technique for high-resolution imaging, providing insights into the structure and composition of various materials. In recent years, tomographic approaches based on simultaneous illuminations of the same sample region from different angles by multiple beams have been developed at micrometre image resolution. Transferring these techniques to the nanoscale is challenging due to the loss in photon flux by focusing the X-ray beam. We present an approach for multi-beam nanotomography using a dual-beam Fresnel zone plate (dFZP) in a near-field holography setup. The dFZP generates two nano-focused beams that overlap in the sample plane, enabling the simultaneous acquisition of two projections from slightly different angles. This first proof-of-principle implementation of the dual-beam setup allows for the efficient removal of ring artifacts and noise using machine-learning approaches. The results open new possibilities for full-field multi-beam nanotomography and pave the way for future advancements in fast holotomography and artifact-reduction techniques.

Spontaneous Coronary Artery Dissection and Tear Repair: A Comprehensive Review of Pathophysiology, Diagnosis, and Therapeutic Interventions

Spontaneous Coronary Artery Dissection (SCAD) represents a rare but significant cause of acute coronary syndrome, predominantly affecting young to middle-aged women without traditional cardiovascular risk factors. The pathophysiology of SCAD involves the spontaneous development of an intimal tear or the formation of an intramural hematoma, leading to coronary artery obstruction and myocardial ischemia. Diagnosing SCAD is challenging due to its varied presentation and the need for high-resolution imaging techniques. Management strategies for SCAD are diverse, ranging from conservative medical therapy to percutaneous coronary intervention (PCI) and, in severe cases, surgical repair. This review aims to provide a detailed analysis of the pathophysiological mechanisms, diagnostic criteria, and therapeutic approaches for the repair of SCAD, highlighting recent advancements in interventional cardiology and surgical techniques.

Acceleration of uterine 3D T2-weighted imaging by compressed SENSE-a multicentre study.

To find the optimal acceleration factor (AF) of the compressed SENSE (CS) technique for uterine isotropic high-resolution 3D T2-weighted imaging (3D-ISO-T2WI). A total of 91 female volunteers from the First Affiliated Hospital of Dalian Medical University, Chu Hsien-I Memorial Hospital and Tianjin Institute of Endocrinology, and The Fourth Hospital of Harbin were recruited. A total of 44 volunteers received uterus sagittal 3D-ISO-T2WI scans on 3.0T MRI device with different CS AFs (including SENSE3, CS3, CS4, CS5, CS6, and CS7), 51 received 3D-ISO-T2WI scans with different degrees of fat suppression (none, light, moderate, and severe), while 4 volunteers received both series of scans. Image quality was subjectively evaluated with a 3-point scoring system. Junction zone signal-to-noise ratio (SNR), contrast-to-noise ratio (CNR), and myometrial SNR were also calculated. Intraclass correlation coefficients were used to analyse the consistency of the measurement results by 2 observers. Analysis of variance test or Friedman rank sum test was used to compare the differences in subjective scores, SNR, and CNR under different AFs/different degrees of fat suppression. Images by AFs of CS3, CS4, and CS5 had the highest SNR and CNR. Among them, CS5 had the shortest scan time. CS5 also had one of the highest subjective scores. There was no significant difference in SNR and CNR among images acquired with different degrees of fat suppression. Also, images with moderate fat suppression had the highest subjective scores. The CS5 combined with moderate fat suppression is recommended for routine female pelvic 3D-ISO-T2WI scan. The CS5 has the highest image quality and has the shortest scan time, which is the best AF. Moderate fat suppression has the highest subjective scores. The CS5 and moderate fat suppression are the best combination for a female pelvic 3D-ISO-T2WI scan.

CFZA camera: a high-resolution lensless imaging technique based on compound Fresnel zone aperture.

In lensless imaging using a Fresnel zone aperture (FZA), it is generally believed that the resolution is limited by the outermost ring breadth of the FZA. The limitation has the potential to be broken according to the multi-order property of binary FZAs. In this Letter, we propose to use a high-order component of the FZA as the point spread function (PSF) to develop a high-order transfer function backpropagation (HBP) algorithm to enhance the resolution. The proportion of high-order diffraction energy is low, leading to severe defocus noise in the reconstructed image. To address this issue, we propose a Compound FZA (CFZA), which merges two partial FZAs operating at different orders as the mask to strike a balance between the noise and resolution. Experimental results verify that the CFZA-based camera has a resolution that is double that of a traditional FZA-based camera with an identical outer ring breadth and can be reconstructed with high quality by a single HBP without calibration. Our method offers a cost-effective solution for achieving high-resolution imaging, expanding the potential applications of FZA-based lensless imaging in a variety of areas.

Feasibility of detecting shadows in disks induced by infall

Observations performed with high-resolution imaging techniques have revealed the existence of shadows in circumstellar disks that can be explained by the misalignment of an inner disk with respect to an outer one. The cause of misalignment, however, is still a matter of debate. In this study, we investigate the feasibility of observing shadows induced by one prominent scenario that may lead to misalignment, which involves the late infall of material onto a protostellar system. In particular, we used previously performed hydrodynamical simulations of such events and we generated flux maps in the visible, near-infrared, submillimeter, and millimeter wavelength ranges using Monte Carlo radiative transfer. Based on those results, we derived synthetic observations of these systems performed with the instruments SPHERE/VLT and ALMA, which we used as a basis for our subsequent analysis. We find that near-infrared observations with SPHERE are particularly well suited for detecting shadows via direct imaging alongside other features such as gaps, arcs, and streamers. On the contrary, performing a shadow detection based on reconstructed ALMA observations is very challenging due to the high sensitivity that is required for this task. Thus, in cases that allow for a detection, sophisticated analyses may be needed, for instance by the utilization of carefully constructed azimuthal profiles, aiding the search for potentially shallow shadows. Lastly, we conclude that a late infall-induced disk misalignment offers a plausible explanation for the emergence of shadows observed in various systems.

All-dielectric super-lattice metasurfaces with fivefold spatial resolution enhancement for structured illumination microscopy.

Structured illumination microscopy (SIM) is a promising imaging technique for high-resolution imaging with a wide field of view. Although a periodic nanostructure is a versatile platform for engineering the spatial frequency of structured illumination patterns in SIM, challenges remain, including artifacts from Fourier space gaps. We designed an all-dielectric super-lattice metasurface (ADSLM) to generate structured illumination patterns with enhanced spatial frequency and broadened spatial frequency coverage with no intermediate frequency gaps. Our numerical simulations reveal that ADSLM-based image reconstruction is capable of producing high-contrast, artifact-free images, resulting in enhanced spatial resolution up to 5.7-fold for coherent SIM at 450 nm. Our results show that the ADSLM-SIM technique may facilitate high-resolution imaging using CMOS-compatible substrates, offering potential for compact miniaturized imaging applications.

The Pivotal Role of Microscopy in Unravelling the Nature of Microbial Deterioration of Waterlogged Wood: A Review

This review focuses on the pivotal role microscopy has played in diagnosing the type(s) of microbial attacks present in waterlogged ancient wooden objects, and to understand the nature and extent of deterioration of such objects. The microscopic journey began with the application of light microscopy (LM) to examine the deterioration of waterlogged woods, notably foundation piles supporting historic buildings, progressing into the use of high-resolution imaging tools (SEM and TEM) and techniques. Although bacteria were implicated in the deterioration of foundation piles, confirmation that bacteria can indeed degrade wood in its native state came when decaying wood from natural environments was examined using electron microscopy, particularly TEM, which enabled bacterial association with cell wall regions undergoing degradation to be clearly resolved. The information base has been a catalyst, stimulating numerous studies in the past three decades or so to understand the nature of microbial degradation of waterlogged archaeological wood more precisely, combining LM, SEM, and TEM with high-resolution chemical analytical methods, including chemical microscopy. The emerging information is aiding targeted developments towards a more effective conservation of ancient wooden objects as they begin to be uncovered from burial and waterlogging environments.

Acute complicated jejunum diverticulitis: a case report with a short literature review.

Jejunal diverticulosis is a rare condition. Most of the time, it is asymptomatic; but it can cause severe complications such as intestinal perforation, mechanical occlusion, and hemorrhage. A patient aged 78 years, with a history of biological aortic valve prosthesis, atrial fibrillation, type 2 diabetes mellitus, and chronic obstructive pulmonary disease, presented in the emergency department for acute abdominal pain in the lower abdominal floor, nausea, and inappetence. Abdominal computed tomography revealed an inflammatory block in the hypogastrium, agglutinated small intestinal loops, fecal stasis, and air inclusions. Pulled mesentery and associated internal hernia are suspected. Exploratory laparotomy was performed, revealing an inflammatory block in the hypogastrium, whose dissection revealed inner purulent collection and the appearance of jejunal diverticulitis, a diagnosis confirmed by histopathological examination. Segmental resection of the jejunum with double-layer terminal-terminal enteroenteric anastomosis, lavage, and drainage was performed. The evolution was favorable. Based on our brief review, the diagnosis of complicated jejunal diverticulosis is difficult and sometimes not accurately established, even by high-resolution imaging techniques, with diagnostic laparotomy being necessary for these situations. Surgical treatment should be considered before severe complications develop.