Taxonomic Recognition Research Articles

PALYNOLOGICAL CHARACTERIZATION OF HERBACEOUS AND WOODY FLORA OF LAHORE UNIVERSITY OF MANAGEMENT SCIENCES, LAHORE, PAKISTAN

The present study reveals palynomorphic characterization of woody and herbaceous flora of Lahore University of Management Sciences, Lahore. In this respect, 67 plant species including 56 dicots, 10 monocots and 1 gymnosperm belonging to 40 different families have been studied by using light microscopy. The present study demonstrates that the pollen morphology of herbaceous and woody flora of LUMS exhibits a huge diversity. The pollen features studied includes structure, volume, aperture, polar length, equatorial diameter, P/E ratio, shape, size, aperture and tectum. Pollen shape was mostly prolate spheroidal but oblate spheroidal, prolate, sub prolate, spheroidal and tricolpate were also observed in some species. Pollen aperture varied from porate to colpate and some species were inaperturate. Pollen tectum has been found reticulate in most species, while psilate, scabrate, verrucate, echinate and striate were also observed. Furthermore, the family name and common name have also been studied. Maximum pollen size is 176.92 µm found in Pinus caraniensis (pinaceae) and minimum pollen size is 88.87µm observed in Morus alba (Moraceae). This work is anticipated to be advantageous in documentation for taxonomic recognition of the plants of Lahore University of Management Sciences, Lahore (LUMS).

Distinct evolutionary lineages of Schistocephalus parasites infecting co-occurring sculpin and stickleback fishes in Alaska.

Sculpins (coastrange and slimy) and sticklebacks (ninespine and threespine) are widely distributed fishes cohabiting 2 south-central Alaskan lakes (Aleknagik and Iliamna), and all these species are parasitized by cryptic diphyllobothriidean cestodes in the genus Schistocephalus. The goal of this investigation was to test for host-specific parasitic relationships between sculpins and sticklebacks based upon morphological traits (segment counts) and sequence variation across the NADH1 gene. A total of 446 plerocercoids was examined. Large, significant differences in mean segment counts were found between cestodes in sculpin (mean = 112; standard deviation [s.d.] = 15) and stickleback (mean = 86; s.d. = 9) hosts within and between lakes. Nucleotide sequence divergence between parasites from sculpin and stickleback hosts was 20.5%, and Bayesian phylogenetic analysis recovered 2 well-supported clades of cestodes reflecting intermediate host family (i.e. sculpin, Cottidae vs stickleback, Gasterosteidae). Our findings point to the presence of a distinct lineage of cryptic Schistocephalus in sculpins from Aleknagik and Iliamna lakes that warrants further investigation to determine appropriate evolutionary and taxonomic recognition.

Re-establishment of Aechmea laxiflora (Bromeliaceae, Bromelioideae) based on morphological and phylogenetic evidence, with notes on its geographical distribution

Background: Previous phylogenetic analyses suggested that Aechmea bracteata is not a monophyletic species, being each variety an individual lineage within the Aechmea bracteata complex.
 Hypothesis: A phylogenetic analysis based on molecular data and a morphological analysis will provide evidence to support the taxonomic recognition of A. bracteata var. pacifica as a distinct species.
 Studies species: A. bracteata var. bracteata, A. bracteata var. pacifica and related species.
 Study site and dates: Mexico to Northern South America; 17 populations from Southeastern and Western Mexico. Analyses were performed between 2021 and 2023.
 Methods: A linear morphometric analysis was conducted with 85 herbarium specimens testing 28 quantitative and three qualitative variables. Potential diagnostic traits were reviewed in living specimens. A phylogenetic analysis was performed with two nDNA markers (ETS and g3pdh) and one cpDNA marker (trnL-F). A total of 373 records were projected into biogeographical provinces of the Neotropics.
 Results: The morphometric analysis allowed to separate each variety into a group, also, nine of the evaluated traits resulted statistically significant through a univariate analysis. Five additional diagnostic traits from the inflorescence and leaves were recognized. According to nDNA both taxa present reciprocal monophyly, however, cpDNA groups A. bracteata var. pacifica with Central American species, showcasing a potential hybrid origin. Geographically, the Sierra Madre del Sur acts as a barrier between both taxa.
 Conclusions: Due to the gathered evidence is possible to recognize A. bracteata var. pacifica as an endemic species to Mexico, reestablishing the basionym A. laxiflora.

Evolution of snake skin: Role of cutaneous tactile corpuscles in Hierophis viridiflavus (Lacépède, 1789)

AbstractThe skin of snakes acts as the first interface between these reptiles and the environment they live in. It plays an important role in the ecology of these organisms, and specialised structures, such as tactile corpuscles, are crucial in the sensing of a wide variety of stimuli. This study, by examining the presence, localisation and colocalisation of serotonin, substance P, PGP 9.5 and tubulin, wants to demonstrate the sensory role that these structures play in the ecology of the Green whip snake Hierophis viridiflavus (Lacépède, 1789). This study presents the first evaluation of nerve and immune molecules (TLR2) in the tactile corpuscles of H. viridiflavius ventral skin, indicating a potential defence role of these structures. These findings provide valuable information on the ecology of snakes, their defence systems, and their evolutionary morphostructural adaptations. Additionally, the identification of these corpuscles could provide valuable support for taxonomic recognition. Furthermore, this study expands the knowledge about stimuli sensing in snakes.

WING PHENOTYPIC DIVERSITY IN STINGLESS BEES GENERA (APIDAE: MELIPONINI) FROM ECUADOR AMAZONIA

Native stingless bees, Meliponini, are the only social bees that produce honey in addition to honeybees. These bees have been managed in meliponiculture and for crop pollination. In Ecuador, meliponiculture studies are scarce and limited by the lack of species recognition. Traditionally the taxonomic recognition of the species is based on morphological characteristics. In Hymenoptera, the wing shape has proven to be a valuable tool in combination with traditional characters. Due to this, the wing shape was evaluated for discriminating morphospecies in Ecuador’s Amazonian meliponaries. A total of 127 specimens grouped into six genera were analyzed: Melipona (Melikerria) sp., Melipona (Michmelia) sp1., Melipona (Michmelia) sp2., Tetragonisca sp., Paratrigona sp., Nannotrigona sp., Scaura sp., and Plebeia sp. The most significant differences are located at the vein intersection: cubital and cubital 1 (Cu + Cu1), posterior margin of the marginal cell (r and Rs), and basal and posterior marginal cell (M and Rs).

Continental mollusks from the Olmos Formation (Upper Cretaceous), Coahuila, Mexico

We describe freshwater mollusks from the Olmos Formation, Campanian, Upper Cretaceous. The results were obtained from several specimens of mollusks, identified with two families of gastropods: Viviparidae (Viviparus sp.) and Physidae (Mesolanistes sp.), and three families of bivalves: Limidae (Pseudolimea sp.), Unionidae (Proparreysia sp., Unio sp., Unionelloides? sp., Plesielliptio sp., and Ostreidae (Ostrea sp.). Based on this taxonomic recognition, along with the associated fossil flora, it was possible to get a preliminary of the environment where the mollusks lived. The interpretation of the paleoenvironment resemble freshwater bodies adjacent to an estuarine system. Furthermore, the mixing of specimens of brackish and freshwater environments is indicative of a transport of some of these mollusks. The general view of the paleoenvironment where the mollusks inhabited is that of a transitional freshwater-estuarine in the Olmos Formation during Campanian times.

Taxonomic notes on the New Zealand flora: the status of four varietal names in Brachyglottis (Asteraceae), Corokia (Argophyllaceae), Mida (Nanodeaceae) and Teucridium (Labiatae)

ABSTRACT Taxonomic names at the rank of variety are often poorly understood and may be misapplied as their taxonomic circumscriptions can be inadequate. These names often fall out of favour or are neglected until they are eventually abandoned, but uncertainty can remain as to their taxonomic or nomenclatural status. While many variety names persist in the New Zealand flora literature and databases, unlike naming a new taxon there are no formal rules or guidelines for not accepting a name and placing it in synonymy. The taxonomic recognition of four varietal names accepted in the Flora of New Zealand Volume 1 (Allan 1961) are assessed against the species to which they are assigned to determine if they warrant continued recognition. These varietal names are Brachyglottis bidwillii var. viridis (Kirk) B.Nord. (Asteraceae), Corokia buddleioides var. linearis Cheeseman (Argophyllaceae), Mida salicifolia var. myrtifolia (A.Cunn.) Allan (Nanodeaceae) and Teucridium parvifolium var. luxurians Cheeseman (Labiatae). For each of these taxa, and the species to which they were assigned, the key characters circumscribing them were analysed. Each of the four varieties are not significantly different from the species to which they have been assigned and are therefore treated as synonyms. While the means of the type species and variety are different, this is not surprising as typically the variety is named for plants that have particularly wide or narrow leaves that mostly occur in a particular part of the range of phenotypic variation. Scatter plots confirm the leaf variation is continuous with no discrete groups. Discriminant analyses demonstrate that specimens were correctly named in only a relatively low percentage of cases, suggesting the measurement ranges are insufficient for reliable recognition and indicative of overlap between the two taxa. Accepted names are listed and a full synonymy is provided, including where necessary the typification of names.

<i>Primula</i> ×<i>chignolensis</i> (Ericales: Primulaceae), a new primrose hybrid discovered in Val Seriana (northern Italy)

A new hybrid of primrose discovered fifteen years ago in the locality Bivacco La Plana, Pre-Alps of Bergamo, above Chignolo, a hamlet of the municipality of Oneta, later also confirmed for the crest of Mount Alben, always in the same municipality, is here described. The parental species turn out to be Primula albenensis Banfi & Ferl. and P. auricula L., the former endemic to the mountains of Bergamo (Mount Alben, Mount Aralalta group and the Ancogno Valley), the latter a European orophyte, here coexisting often together on limestone faces. The new finding shows constant morphology in its stands, and there is no evidence of either "hybrid swarming" or introgression to exclude the total sterility of the population and this occurrence should be considered result of a process of inter parentes hybrid speciation (nothospecies) deserving of taxonomic recognition.

Coastal dwarf variants of the red swamp Banksia (Proteaceae) represent parallel ecotypic evolution rather than a divergent evolutionary lineage of single origin

Abstract Morphological variation is common within widely distributed plant species and often raises interesting taxonomic and evolutionary questions. We sampled eight typical inland populations and two coastal dwarf populations of Banksia occidentalis to assess whether the coastal variant warrants taxonomic recognition. Genomic (single nucleotide polymorphisms) and morphometric analyses were undertaken to assess the variation within and between variants. Genetically, the coastal populations were more closely related to geographically adjacent populations of the typical variant than they were to each other, indicating that each coastal population has originated independently. Morphologically, the coastal populations also did not represent a cohesive phenotypic group, with both falling outside the range of variation for the typical variant, but also not overlapping each other. Wide morphological, but not genomic, variation was identified along a coastal–inland transect. Combined, these data indicate that the morphological variation in these coastal populations is the result of parallel ecotype evolution from experiencing similar environmental conditions and does not represent the diversification of a distinct evolutionary lineage that would have warranted taxonomic recognition. Our study highlights the consequences of strong selection pressures in marginal environments and provides fascinating insight to the long evolutionary history of the flora in this ancient Australian landscape.

On the importance of types and the perils of en passant taxonomy: a brief history of the typification of Coluber naja Linnaeus, 1758 (Serpentes: Elapidae) and its implications, with the designation of a lectotype.

In response to the recent in passing (en passant) taxonomic decision to split Naja naja (Linnaeus) and recognise the Sri Lankan populations as a separate species, N. polyocellata Deraniyagala, we analyse the evidence underlying the proposal and its nomenclatural implications. The proposed split is weakly supported by the available evidence, so that retaining N. naja as a single species seems appropriate until further analysis. Moreover, the proposal raises several issues concerning types, type locality and nomenclature. Linnaeus description of Coluber naja was based on a single preserved specimen seen by him (now lost) and several illustrations in Sebas Thesaurus. The specimens that were the basis of these illustrations constitute part of the type series. Two of the latter specimens, ZMB 2795 and 2796, have been rediscovered in the collections of the Museum fr Naturkunde, Berlin. Here, we describe them, and determine that both are of Sri Lankan origin. To settle the question of the type and type locality of this iconic taxon, we designate ZMB 2796 as lectotype for the species, thereby implicitly restricting the type locality to Sri Lanka. The name polyocellata thus becomes a subjective junior synonym of Coluber naja, and the name Naja brasiliensis Laurenti, 1768 an objective junior synonym thereof. Any taxonomic recognition of additional diversity within N. naja would thus require the renaming of Indian, not Sri Lankan spectacled cobras, but should await a significant body of convincing evidence. We caution against taxonomic decisions taken in passing, based on limited evidence and without in-depth assessment of their nomenclatural implications.

Incipient speciation in allopatric Etheostoma rupestre (Percidae: Etheostomatinae) lineages, with the description of three new subspecies.

In recent years, new species descriptions for the North American darters have proliferated. Most species concepts accepted by contemporary ichthyologists require that a valid species be both monophyletic and diagnoseable, yet many lineages exhibit modal or range differences in morphological characteristics without individuals being diagnosable. Such scenarios present difficulties with regards to proper taxonomic recognition of divergent lineages and often prohibit appropriate conservation action. Following the example of recent authors, we provide meristic, geometric morphometric, and pigmentation data to support the recognition of three subspecies of Etheostoma rupestre, a species endemic to the Mobile Basin. These morphological data cohere with previous genetic work for E. rupestre. The nominate subspecies Etheostoma rupetsre rupestre (Tsais Rock Darter) is endemic to the Tombigbee River and Black Warrior River watersheds in Alabama and Mississippi and is characterized by having lower numbers of lateral blotches, lower range and mean of lateral line scales, lower modal number of scales above the lateral line, and lower degrees of nape squamation than other subspecies. Etheostoma rupestre piersoni (Shamrock Darter), ssp. nov., is endemic to the Cahaba and Alabama River Watersheds in Alabama and is characterized by intermediate counts of lateral blotches and higher scale counts and nape squamation than E. r. rupestre. Etheostoma rupestre uphapeense (Jade Darter), ssp. nov., is restricted to several small, disjunct populations in the Coosa and Tallapoosa watersheds in Alabama, Georgia, and Tennessee. Etheostoma r. uphapeense is characterized by having a higher mean number of lateral blotches than both other subspecies and higher scale counts than E. r. rupestre. While E. r. rupestre and E. r. piersoni are widespread and abundant within their respective ranges, E. r. uphapeense has a disjunct range and is often uncommon where it occurs. Etheostoma r. uphapeense should be monitored where it occurs to discern population trends.

The importance of cryptic diversity in the conservation of wide-ranging species: The red-footed tortoise Chelonoidis carbonarius in Colombia.

Wide-ranging species are seldom considered conservation priorities, yet they have the potential to harbour genetically deeply differentiated units across environments or ecological barriers, including some that warrant taxonomic recognition. Documenting such cryptic genetic diversity is especially important for wide-ranging species that are in decline, as they may comprise a set of even more endangered lineages or species with small distributions. However, studies of wide-ranging species, particularly when they cross political borders, are extremely challenging. One approach to overcoming these challenges is to conduct detailed local analyses in combination with less detailed, range-wide studies. We used this approach with the red-footed tortoise (Chelonoidis carbonarius), a threatened species likely to contain cryptic diversity given its vast range and the distinctive ecoregions that it inhabits. Previous single-gene molecular studies indicated the presence of at least five lineages, two of which occur in different ecoregions separated by the Andes within Colombia. We used a comprehensive genomic analysis to test the hypothesis of cryptic diversity within the single jurisdiction of Colombia. We used a combination of restriction-site-associated DNA sequencing and environmental niche modelling to provide three independent lines of evidence that support the presence of important cryptic diversity that may deserve taxonomic recognition: allopatric reproductive isolation, local adaptation and ecological divergence. We also provide a fine-scale genetic map with the distribution of conservation units in Colombia. As we complete ongoing range-wide analyses and make taxonomic adjustments, we recommend that the two lineages in Colombia be treated as separate units for conservation purposes.

Pseudosperma pamukkalense (Inocybaceae: Agaricomycetes), a new species from Turkey

A new species, Pseudosperma pamukkalense, discovered in Pinus nigra subsp. pallasiana forests is described from the Mediterranean climate zone of south-western Turkey. The novel species is recognized by the presence of pale straw to brownish yellowish pileus, mingled with reddish-brownish streaks with age, smooth to finely innately fibrillose pileus surface, whitish stipe with mainly cylindrical to clavate caulocystidia, large subphaseoliform basidiospores, and mostly narrowly to broadly clavate cheilocystidia. The description of the new species is based on morphological characters, and illustrations, line drawings and comparisons with allied species are provided. A molecular phylogenetic analysis of the internal transcribed spacer (ITS) and the nuclear 28S rRNA gene (nrLSU) regions supported the taxonomic recognition of the new species which was on a separate branch within the genus Pseudosperma.

Pollination by native bees achieves high fruit quantity and quality of highbush blueberry: a sustainable alternative to managed pollinators

IntroductionBlueberry is one of the most relevant buzz-pollinated crops worldwide, and Chile is the most important global producer of fresh blueberries during wintertime in the Northern Hemisphere. Non-buzzing bees, such as honey bees, may provide suboptimal services compared with bees capable of buzz pollination. The widely held contention that honey bees are inferior pollinators of blueberries drives the industry to place pressure on governments to allow bumblebee (Bombus terrestris) importation for pollination. However, the introduction of B. terrestris generates environmental problems in Chile by competing with and transmitting parasites to local bees. Despite some native Chilean bees being recently recognized as efficient pollen vectors of blueberry crops, no study has evidenced the influence of their visits on fruit yield. Therefore, we aimed to evaluate the native Chilean floral visitors' performance to improve the quantity and quality of highbush blueberry in comparison to the performance of managed visitors.MethodsPer-visit pollination performance (fruit set and fruit quality) and visitation frequency were measured, and the performance of buzzing behavior by flower visitors was evaluated in four cultivars grown in five blueberry orchards located in southern Chile.ResultsWe found that fruit set and weight were highly influenced by floral visitor taxon. Some native bee species can greatly improve the fruit set and fruit quality (greater weight) of the highbush blueberry cultivars. For instance, one single visit of C. occidentalis can increase fruit weight by a factor of 1.8 compared to an A. mellifera visit; however, visits of halictids and syrphids resulted in lower fruit sets than those of unvisited flowers. However, we found that the occurrence of sonication behavior alone was not a predictor of higher fruit set and fruit weight of highbush blueberry cultivars. Consequently, the taxonomic recognition of floral visitors, ideally to the species level, is still needed to distinguish the most efficient fruit yield promoters of blueberry.DiscussionThe conservation of the biotic pollinators, especially native pollinators, would improve blueberry fruit quality and is likely to improve overall crop productivity.

Automatic acoustic recognition of pollinating bee species can be highly improved by Deep Learning models accompanied by pre-training and strong data augmentation.

Bees capable of performing floral sonication (or buzz-pollination) are among the most effective pollinators of blueberries. However, the quality of pollination provided varies greatly among species visiting the flowers. Consequently, the correct identification of flower visitors becomes indispensable to distinguishing the most efficient pollinators of blueberry. However, taxonomic identification normally depends on microscopic characteristics and the active participation of experts in the decision-making process. Moreover, the many species of bees (20,507 worldwide) and other insects are a challenge for a decreasing number of insect taxonomists. To overcome the limitations of traditional taxonomy, automatic classification systems of insects based on Machine-Learning (ML) have been raised for detecting and distinguishing a wide variety of bioacoustic signals, including bee buzzing sounds. Despite that, classical ML algorithms fed by spectrogram-type data only reached marginal performance for bee ID recognition. On the other hand, emerging systems from Deep Learning (DL), especially Convolutional Neural Networks (CNNs), have provided a substantial boost to classification performance in other audio domains, but have yet to be tested for acoustic bee species recognition tasks. Therefore, we aimed to automatically identify blueberry pollinating bee species based on characteristics of their buzzing sounds using DL algorithms. We designed CNN models combined with Log Mel-Spectrogram representations and strong data augmentation and compared their performance at recognizing blueberry pollinating bee species with the current state-of-the-art models for automatic recognition of bee species. We found that CNN models performed better at assigning bee buzzing sounds to their respective taxa than expected by chance. However, CNN models were highly dependent on acoustic data pre-training and data augmentation to outperform classical ML classifiers in recognizing bee buzzing sounds. Under these conditions, the CNN models could lead to automating the taxonomic recognition of flower-visiting bees of blueberry crops. However, there is still room to improve the performance of CNN models by focusing on recording samples for poorly represented bee species. Automatic acoustic recognition associated with the degree of efficiency of a bee species to pollinate a particular crop would result in a comprehensive and powerful tool for recognizing those that best pollinate and increase fruit yields.

Legal Limbo and Caste Consternation: Determining Kayasthas’ Varna Rank in Indian Law Courts, 1860–1930

Abstract This article explores how colonial law in India interacted with the construction of caste rank (varna) between 1860 and 1930. It specifically tracks contestations over Kayasthas’ legal varna rank in northern and eastern India through various inheritance disputes, threading them together to shed light on how courts sought to anchor their interpretations of Hindu law around the Indian jurisprudential conceptions of varna. It examines the successes and failures of Kayasthas to have favorable legal rulings that would uphold their status as “twice-born”/dvija, demonstrating that colonial law was limited in its ability (and often indifferent) to construct caste ranks. Inconsistent ruling in provincial courts pushed Kayasthas to seek taxonomic recognition as “twice-born” in the colonial census, demonstrating how colonial law and taxonomy intersected in novel ways. This article argues that by taking a novel approach to Indian social history through the prism of law, we can enrich our understanding of how modern notions of caste and social rank were constructed in colonial India.

Taxonomic species recognition should be consistent.

Taxonomic species recognition should be consistent.

Aloiampelos tenuior var. ernstii, a new orange-flowered variety of rambling aloe (Asphodelaceae subfam. Alooideae)

A red- and several yellow-flowered variants of Aloiampelos tenuior (Asphodelaceae subfam. Alooideae), a species of scrambling aloe, are well known, including in cultivation, and apart from the autonymic variety, at least four others have been given taxonomic recognition at that rank. The yellow-flowered varieties of A. tenuior are predominantly, but not exclusively, based on vegetative characters, while the red-flowered variety is distinguished by its flower colour, as well as vegetative characters. The orange-flowered form of A. tenuior, which is much less common in cultivation than A. tenuior var. tenuior or A. tenuior var. rubriflora, is here described as A. tenuior var. ernstii.

An integrative insight into the diversity, distribution, and biogeography of the freshwater endemic clade of the Ponticola syrman group (Teleostei: Gobiidae) in the Caucasus biodiversity hotspot.

Freshwater habitats of the Caucasus biodiversity hotspot represent a center of endemism for the gobiid genus Ponticola Iljin, 1927. Hitherto, large‐scale molecular studies, owing to restricted taxon and geographical sampling, have failed to give an elaborate picture of diversity and evolutionary history of these species. Here, to contribute to filling this gap, we assessed taxonomic diversity, phylogeography and evolutionary history for the south Caspian populations of Ponticola presently classified as P. iranicus and P. patimari, using an integrative taxonomic approach comprising an entire geographic range sampling, and analyses of mitochondrial DNA haplotypes, the head lateral line system, otolith shape, and meristic and morphometric variation. All freshwater samples of the P. syrman group belong to a monophyletic clade with two main subclades: a small subclade confined to the upper Sefidroud sub‐basin including the type locality of P. iranicus and a large subclade with three geographically constrained haplogroups (Hg1, Hg2, and Hg3), comprising the rest of the distribution. Hg1 showed an eastern distribution including the type locality of P. patimari, while Hg2 and Hg3 are sister groups with central and western‐central distributions, respectively. The freshwater clade diverged from P. syrman during the Tyurkyanian low stand (~150 m b.s.l. lasting ~0.1 Myr), while the divergence of P. iranicus and P. patimari and radiations within P. patimari took place during the Bakunian high stand (up to 50 m a.s.l. lasting ~378–480 kya). Species delimitation analyses indicated two distinct species, corresponding to each main subclade. Although the otolith shape and lateral line analyses did not reflect with phylogeographic pattern, PCA and DFA plots of meristic and morphometric data showed a clear separation of the two major subclades corresponding to P. iranicus and P. patimari, suggesting the presence of significant morphological variation meriting formal taxonomic recognition. Overall, our findings (i) reveal the presence of two freshwater endemic species in the P. syrman group, and pending further investigation, hypothesize the presence of a third cryptic species; (ii) revise and document a narrow distributional range and low diversity for P. iranicus, in contrast to a wider distributional range and high diversity for P. patimari; (iii) suggest that the climatic oscillations of the Pleistocene were associated with the cladogenesis within the P. syrman group; and (iv) allowed for the recognition of conservation units and proposition of management measures.

Accelerating the discovery of rare tree species in Amazonian forests: integrating long monitoring tree plot data with metabolomics and phylogenetics for the description of a new species in the hyperdiverse genus Inga Mill.

In species-rich regions and highly speciose genera, the need for species identification and taxonomic recognition has led to the development of emergent technologies. Here, we combine long-term plot data with untargated metabolomics, and morphological and phylogenetic data to describe a new rare species in the hyperdiverse genus of trees Inga Mill. Our combined data show that Inga coleyana is a new lineage splitting from their closest relatives I. coruscans and I. cylindrica. Moreover, analyses of the chemical defensive profile demonstrate that I. coleyana has a very distinctive chemistry from their closest relatives, with I. coleyana having a chemistry based on saponins and I. cylindrica and I. coruscans producing a series of dihydroflavonols in addition to saponins. Finally, data from our network of plots suggest that I. coleyana is a rare and probably endemic taxon in the hyper-diverse genus Inga. Thus, the synergy produced by different approaches, such as long-term plot data and metabolomics, could accelerate taxonomic recognition in challenging tropical biomes.