Beef Taste Research Articles

Targeted-metabolomic and untargeted-proteomic approaches reveal the effects of muscle fibre type and postmortem ageing on taste-active compounds in beef

The taste of beef is caused by taste-active compounds detected in the mouth during mastication. We hypothesised that the concentration of taste-active compounds in beef is influenced by muscle-fibre-type and postmortem ageing. To test this, and unravel the underlying mechanisms, we investigated the taste-active compounds, and proteomic profiles, in beef masseter [oxidative muscle, all type I fibres) and cutaneous trunci (glycolytic muscle, mostly type II fibres) before and after 14-days postmortem ageing. Our results showed that nucleotides were initially higher and degraded slower in cutaneous trunci (P < 0.05 for both), which could be explained by the profile of nucleotide metabolism enzymes. In contrast, free amino acids were initially higher and increased more in masseter compared to cutaneous trunci (P < 0.05 for all), which might be explained by the profile and activity of proteases in these two muscles. Our results indicate the taste of beef is affected by the muscle-fibre-type and postmortem ageing.

Study on the Correlations between Quality Indicators of Dry-Aged Beef and Microbial Succession during Fermentation.

Dry-aged beef has been long favored by people due to its unique flavor and taste. However, the inner relationship between its overall quality formation and microbial changes during dry aging has not yet received much attention and research. To deeply reveal the forming mechanism of the unique flavor and taste of dry-aged beef, correlations between its three main quality indicators, i.e., texture, free amino acids (FAAs), volatile flavor compounds (VFCs), and microbial succession were analyzed in this study. The results showed that Staphylococcus spp. and Macrococcus spp. were key strains that influenced the total quality of dry-aged beef and strongly correlated with chewiness, hardness, and sweet FAAs (Ala), providing beef with unique palatability and taste. Additionally, among VFCs, Staphylococcus spp. and Macrococcus spp. showed a strong correlation with octanal and heptanal, and meanwhile, those highly correlated with nonanal, pentanol, and oct-1-en-3-ol were Debaryomyces spp., Psychrobacter spp., and Brochothrix spp., respectively, providing beef with a unique flavor. Staphylococcus spp. was proposed to be the dominant genus for dry-aged beef. This study provides valuable reference for the understanding of the role of microorganisms involved in dry aging.

Quality Properties and Flavor-Related Components of Beef Longissimus Lumborum Muscle from Four Korean Native Cattle Breeds.

This study was carried out to assess the quality properties, components associated with taste and aroma of beef as a function of breed. For this purpose, steers from four Korean native cattle breeds: Hanwoo (n=10), Chikso (n=10), black Hanwoo (n=12, BHW) and Jeju black cattle (n=12, JBC) were used. The steers all were raised under identical conditions and finished at a similar age of around 30-months old. Following 24 h of slaughter, all longissimus lumborum muscles were collected and used for analysis of meat quality, fatty acids, and flavor-related components (metabolic compounds, free amino acids, and aroma volatiles). The Hanwoo presented a significantly higher intramuscular fat content (IMF, 22.85%) than the BHW (11.78%), Chikso (9.25%), and JBC (9.14%; p<0.05). The meat of Hanwoo breed showed lighter and redder color, and lower shear force value (p<0.05). The JBC presented a "healthier" fatty acid profiles as it had a higher total unsaturated fatty acids content (p<0.05). With regard to flavor-related components, Hanwoo also had higher total contents of free amino acids and metabolites associated with umami and sweet tastes, and fat-derived volatile compounds (aldehydes, alcohols, and ketones) associated with fatty aroma. It may be concluded that there was a considerable difference in the meat quality properties among breeds. The variations of IMF content and flavor-related components may be the main factors contributing to the typical flavors of beef among the four Korean native cattle breeds.

DRY-AGED BEEF: COLOR PARAMETERS AND SENSORY CHARACTERISTICS

Research work involves the instrumental analysis of the change in color (L*, a*, b*) and the sensory characteristics of the Simmental beef, parts of the carcass: T-bons, received from the local farm, during the dry-aging period for 0, 14, 21, 28, 35 days and for the roasted aged beef at high and very high temperatures. The analyzed beef samples presented uniform results for the chromatic parameters during the dry-aging period, being influenced by the values of meat pH and the water-holding capacity, namely: for the dry-aged meat, the L* parameter values indicate an increase in the brightness of the meat, for the a* parameter, decreasing values were established, which indicates a reduction in the oxygenation of the meat, a reduction of oxymyoglobin in meat, responsible for the intense red color and for the b* coordinate a slight decrease in values was observed, oriented to yellow color. For roasted beef parameter L* decreases due to the reduction of the beef moisture during the roasting process. The redness of beef a* decreases in intensity primarily depending on the beef aging period but also with the increase in the roasting temperature, due to the drastic degradation of myoglobin. The b* parameter also decreases, possibly due to the increase in metmyoglobin content during heat treatment. The sensory characteristics of the dry aged beef have been considerably improved due to the increase in the pH of the meat, which attracts the activation of meat-specific enzymes that contribute to the accumulation of secondary compounds with a major impact on the beef taste, aroma and juiciness. These aspects are later reflected on the sensory properties of the roasted aged beef, being intensified by the heat treatment process.

Strategi Komunikasi Pemasaran Melalui Media Sosial Instagram (Studi kasus Pada Online Shop SEIWAY di Tangerang selatan)

Sales methods have developed from bartering, direct sales in stores, door to door marketing (salesmen), advertising on television and radio, entering the digital marketing era in the 2000s. Digital (online) marketing involves promoting products or services using social media technology, connecting producers directly with consumers, allowing for daily advertising, uploading product videos, and direct online sales where consumers can interact and transact via live chat on online platforms. Social media consisting of Facebook, Instagram, WhatsApp, and online markets such as Shopee, Lazada, and Tokopedia. The focus of this research is online sales via the social media platform Instagram. The research subject is Small and Medium Enterprises (MSMEs) in South Tangerang which are famous for Sei Sapi SeiWay, a new processed beef product with the taste of roast beef which is popular among young people. Researchers are interested in discussing digital marketing in SeiWay sales in South Tangerang. The aim of this research is to understand SeiWay's marketing communication strategy through the social media platform Instagram. The research methodology used is qualitative research aimed at finding insights that cannot be obtained through statistical procedures or other quantification methods. The results is SEI WAY's sales experienced an increase in sales, both sales of food products and franchises, by participating in marketing programs on Instagram social media. However, not all advertising programs on Instagram are followed due to limited budget to place advertisements every month on Instagram. The recommendation for SeiWay is hire a marketing digital team’s for increase sales via media soscial.

Inosine 5′- monophosphate derived umami taste intensity of beef determination by electrochemistry and chromatography

The umami sensation contributes to beef taste and acceptability. Inosine 5′- monophosphate (IMP), the most abundant 5′-ribonucleotide in meat, is known to impart an umami taste without the undesired side effects commonly associated with glutamate. Nevertheless, the investigation of IMP's role in beef flavor has thus far been overlooked. Traditional methods for detecting IMP have relied on liquid chromatography coupled with ultraviolet spectroscopy or mass spectrometry techniques. However, these methods are not practical for production settings due to the complexity and resource demands of sophisticated laboratory techniques. Alternative methods like cyclic voltammetry might offer more practical solutions for rapidly detecting IMP. The objectives of this study were to evaluate the efficiency of using electrochemistry and chromatography on differentiating beef strips spiked with different IMP contents. The IMP threshold was 0.30 mM determined by a trained panel using the Best Estimates Threshold method. Beef strip steaks of USDA Prime, Choice, and Select were spiked at 0.30 and 0.60 mM of IMP, based on green weight and an estimated moisture content of 65%. In this study, differences in the IMP content of steaks were not detected by liquid chromatography-mass spectrometry. However, the cyclic voltammetry approach differentiated IMP concentrations at 0.50 mM or above in aqueous solutions and subsequentially meat extracts from the buffered blank solutions. In conclusion, cyclic voltammetry holds potential as a rapid and effective approach for detecting IMP in beef and other meat products, offering promising applications for future research.

Effect of dry-ageing with Mucor flavus on beef taste and aroma

Okinawa Industrial Technology Centre succeeded in isolating the mould strain, Mucor flavus, creating savoury odours during beef ageing. Several companies already produce dry-aged beef products using the isolated mould strain in Okinawa. It will be a new food content as the Okinawan brand for tourists from all over the world. After trimming, each amino acid in the meat surface was analysed by LCMS and aroma volatile compounds from the headspace were analysed by solid-phase micro extraction followed by GCMS to reveal the effect of dry-ageing with M. flavus on beef taste and aroma. Each amino acid in the surface of dry-ageing beef with the mould increased. It was hypothesised that there is a gradient of moisture content from the surface of the edible part to the inner side of the dryaged beef, where amino acids are easily generated from the protein degradation. Amino acids react with sugars by heating, resulting in a savoury flavour. Besides, the peak number of volatile compounds using GCMS was more detected in dry-aged beef with mould than that in dry-aged beef without mould. There is a high possibility that M. flavus can produce higher polar compounds in beef meat. Several aldehydes detected only in dryaged beef with the mould were related to savoury flavours, such as nutty and boiled meat.

Composition and taste of beef, pork, and duck meat and bioregulatory functions of imidazole dipeptides in meat

This study quantified the nutritional components and imidazole dipeptide levels of commercially available meats (beef, pork, and duck), and their effects on taste were quantified via taste recognition devices. Although meat and its products are considered high-risk diets, meat components, such as imidazole dipeptides, exert bioregulatory functions. Further, considering their bioregulatory function, commercial meats’ antioxidant activity and vascular endothelial function were examined. Characteristic variations in nutritional components were observed depending on the type and part of meat analyzed. These components affected the taste and texture of meat. The main imidazole dipeptides detected were anserine (duck meat) and carnosine (beef and pork). Meat with larger quantities of total imidazole dipeptide demonstrated better sensory test results. Therefore, anserine and carnosine effects on taste were determined using a taste recognition device; carnosine alone produced a noticeably bitter taste, whereas adding anserine reduced bitterness and enhanced umami taste. In a few cases, cooking enhanced the quantity of carnosine and/or anserine and their antioxidant activities. We demonstrated the ability of imidazole dipeptides, particularly anserine, to improve nitric oxide production in vascular endothelial cells. This study provides essential information for health-conscious consumers to develop high-quality, functional meat products.

Characterization of stewed beef by sensory evaluation and multiple intelligent sensory technologies combined with chemometrics methods

Though stewed beef is favored by consumers, the impact of the domestic high-pressure stewing method on beef has received little attention. This study characterized the beef cooked under varied pressures in the household pressure cooker by analytical instruments, sensory evaluation, and multiple intelligent sensory technologies. Totally, 165 volatile compounds were identified by SPME-GC-TOF-MS, with more detected in samples with higher pressure. The glutamic acid contributed significantly to the umami taste of beef (TAV > 1.25). The meaty and fatty odor, hardness, chewiness, and sweet taste contributed to the overall liking of stewed beef (P < 0.05). The multiple-target BPNN model based on fused data from multiple intelligent sensory technologies could simultaneously predict sensory perception intensities with a satisfying performance (R2 > 0.9340), but could not efficiently predict subjective overall liking scores. The study guides the domestic cooking of beef stew and quantitative sensory prediction based on multiple intelligent sensory techniques.

Identification of the hub genes related to adipose tissue metabolism of bovine.

Due to the demand for high-quality animal protein, there has been consistent interest in how to obtain more high-quality beef. As well-known, the adipose content of beef has a close connection with the taste and quality of beef, and cattle with different energy or protein diet have corresponding effects on the lipid metabolism of beef. Thus, we performed weighted gene co-expression network analysis (WGCNA) with subcutaneous adipose genes from Norwegian red heifers fed different diets to identify hub genes regulating bovine lipid metabolism. For this purpose, the RNA sequencing data of subcutaneous adipose tissue of 12-month-old Norwegian red heifers (n = 48) with different energy or protein levels were selected from the GEO database, and 7,630 genes with the largest variation were selected for WGCNA analysis. Then, three modules were selected as hub genes candidate modules according to the correlation between modules and phenotypes, including pink, magenta and grey60 modules. GO and KEGG enrichment analysis showed that genes were related to metabolism, and participated in Rap, MAPK, AMPK, VEGF signaling pathways, and so forth. Combined gene interaction network analysis using Cytoscape software, eight hub genes of lipid metabolism were identified, including TIA1, LOC516108, SNAPC4, CPSF2, ZNF574, CLASRP, MED15 and U2AF2. Further, the expression levels of hub genes in the cattle tissue were also measured to verify the results, and we found hub genes in higher expression in muscle and adipose tissue in adult cattle. In summary, we predicted the key genes of lipid metabolism in the subcutaneous adipose tissue that were affected by the intake of various energy diets to find the hub genes that coordinate lipid metabolism, which provide a theoretical basis for regulating beef quality.

Quality Organoleptic Se’i Beef Given Schleichera oleosa Bark Extract on Different Volums

This study aims to determine the organoleptic quality of beef Se’i which was given Schleichera oleosa bark extract at different levels. Se’i beef was processed by adding 0 mL of Schleichera oleosa bark extract (without the addition of extract), 20 mL, 30 mL and 40 mL, then the beef Se’i was smoked in a smoker house for ± 1 hour at a temperature of 80-90˚C. Se’i beef was then tested by 30 semi-trained panelists who were panelists who often consumed beef Se’i. The variables observed in this study included color, aroma, texture and taste of beef Se’i. The data obtained were analyzed statistically with a nonparametric alternative test, namely Kruskal Wallis. If the Kruskal Wallis test showed a significant difference (p&lt;0.05), then it was continued with the Post Hoc Test using the Mann Whitney test. Panelists responded to the color of beef Se’i best in the addition of 30 mL of Schleichera oleosa bark extract with an average value of 4.37. Beef Se’i with the addition of Schleichera oleosa bark extract produces a reddish color compared to Se’i without the addition of Schleichera oleosa bark extract which has a more blackish color. The best beef Se’i aroma was shown in the addition of 40 mL of Schleichera oleosa bark extract at 5.30 followed by the addition of 30 mL and 20 mL of extract. 30 mL of Schleichera oleosa bark extract provides a distinctive taste that is favored by panelists. The best taste of beef Se’i in the addition of 30 mL of Schleichera oleosa bark extract was 4,70 followed by the addition of 20 mL of Schleichera oleosa bark extract, which had a second average of 4.50 and 40 mL, which was 4.16. Meanwhile, the lowest mean value was in the treatment without the addition of Schleichera oleosa bark extract, which was 4.00. Panelists assessed the best texture of beef Se’i produced in the addition of 30 mL of Schleichera oleosa bark extract, which was 5.26. Se’i beef with the addition of 40 mL of Schleichera oleosa bark extract had a second average of 5.23 and 20 mL, which was 4.50. Meanwhile, beef Se’i without the addition of Schleichera oleosa bark extract had the lowest average of 4.06.

Sensory Properties and Main Differential Metabolites Influencing the Taste Quality of Dry-Cured Beef during Processing.

This study adopted widely targeted high-performance liquid chromatography-tandem mass spectrometry (HPLC-MS/MS) metabolomics and multivariate data analysis methods to evaluate the correlation between changes in metabolites and their taste formation in dry-cured beef during processing. The physicochemical profile changed significantly in the maturity period (RG), especially due to the continuous hydrolysis and oxidation of proteins. The sensory characteristic of dry-cured beef was highest in saltiness, umami, overall taste, and after-taste in RG. Overall, 400 metabolites were mainly identified, including amino acids, peptides, organic acids, and their derivatives, nucleotides, and their metabolites, as well as carbohydrates. Cysteine and succinic acid were significantly up-regulated during the process of dry-curing beef compared to the control group (CG). Moreover, glutamine and glutathione were significantly down-regulated in the fermentation period (FG) and in RG. Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment analysis revealed that glyoxylate and dicarboxylate metabolism, glutathione metabolism, alanine, aspartate, and glutamate metabolism, arginine biosynthesis, taurine, and hypotaurine metabolism were the main metabolic pathways influencing the taste of dry-cured beef during processing. Results of correlation analysis revealed that umami is positively correlated with salty, L-cysteine, L-arginine, inosine, creatinine, and succinic acid. Our study results provide a better understanding of the changes in taste substances and will contribute to quality evaluation of dry-cured beef.

Impact of plant-based meat alternatives on cattle inventories and greenhouse gas emissions

New plant-based meat (PBM) alternatives that aim to mimic the taste and texture of beef could have significant economic, environmental, and animal welfare impacts if they replace traditional animal-based meats and reduce livestock production. Whether PBM alternatives can achieve these ends depends on the extent to which consumers are willing to substitute for PBM alternatives, the structure of the meat industry, and the inter-linkages of the livestock industry with the other parts of the economy. We construct and calibrate an economic model to estimate how a reduction in PBM prices, or increase in demand for PBM, in the United States affects cattle production. For every 10% reduction in price or increase in demand for PBM, we estimate U.S. cattle production falls approximately 0.15%, U.S. cattle producers’ economic welfare falls by $300 million year−1, and U.S. consumer welfare rises by $513 million year−1. Key variables affecting model outcomes include the supply elasticity of cattle, the share of the total cost of cattle used to produce ground beef, and cross price-elasticity of demand between PBM and ground beef. Increases in U.S. demand for PBM alter trade patterns, leading to a reduction of beef imports and an increase in beef exports, a phenomenon that further reduces global greenhouse gas emissions and land use given the relative efficiency of U.S. beef production. For every 10% reduction in the price of PBM alternatives, we estimate that the global reduction in emissions is equivalent to 0.34% of U.S. emissions from beef production and 1.14% when including reduced land-use change emissions. Even substantial reductions in prices of PBM alternatives are unlikely to have substantive impacts on the U.S. cattle population and emissions, suggesting the need to also pursue alternative mitigation strategies, such as innovations to reduce the methane emissions per head.

Impact of Plant-Based Meat Alternatives on Cattle Inventories and Greenhouse Gas Emissions

New plant-based meat alternatives that aim to mimic the taste and texture of beef could have significant economic, environmental, and animal welfare impacts if they replace animal-based meats and reduce livestock production. Whether plant-based meat alternatives can achieve these ends depends on the extent to which consumers are willing to substitute for plant-based meat alternatives, the structure of the meat industry, and the inter-linkages of the livestock industry with the other parts of the economy. We construct and calibrate an economic model to estimate how a reduction in plant-based meat prices, or increases in demand for plant-based meat, in the United States affect cattle production. For every 10% reduction in price or increase in demand for plant-based meat alternatives, we estimate U.S. cattle production falls approximately 0.15%, U.S. cattle producers’ economic welfare falls by $300 million/year, and U.S. consumer welfare rises by $513 million/year. Increases in U.S. demand for plant-based meat alter trade patterns, leading to a reduction of beef imports and an increase in beef exports, a phenomenon that further reduces global greenhouse gas emissions and land use given the relative efficiency of U.S. beef production. For every 10% reduction in the price of plant-based meat alternatives, we estimate that the global reduction in emissions is equivalent to 0.41% of U.S. emissions from beef production and 1.4 % when including carbon sequestration from reforestation of abandoned cropland and pasture and reduced land-use change emissions.1 Even substantial reductions in prices of plant-based meat alternatives are unlikely to have substantive impacts on the U.S. cattle population and emissions, suggesting the need to also pursue alternative mitigation strategies, such as innovations to reduce the methane emissions per head.

A non-destructive detection method for evaluating beef taste quality based on electrochemical PVC membrane sensor

A polyvinyl chloride (PVC) membrane sensor was developed to accurately detect beef taste quality grades. The beef taste was identified by the Fisher discriminant and verified by sensory evaluation. The PVC membrane sensor with the maximum open circuit potential (OCP) value and excellent performance was composed of 0.4 mL of dibutyl phthalate (DBP), 0.25 mL of lauryl ether phosphate, 3.6 mg of magnesium stearate and 6 mg of thermoplastic polyurethanes (TPU). Texture analyzer tests showed that the membranes showed desired physical properties, and the elasticity, viscosity and cohesion of the PVC membrane sensor were 4.74 mm, 2.17 mJ and 1.89 respectively. Scanning electron microscopy (SEM) showed the electrode immersed for 6 h without separation with PVC membrane. The coefficient of variation was less than 10% with a storage period of 7 months. Sensory evaluation showed that the beef sensory rated grades array determination by the Fisher discriminant method to verify the sensitivity of ammonia-sensitive substance-modified PVC membrane sensor had an accuracy of 93.3%.

Improving the quality of sous-vide beef from Holstein-Friesian bulls by different marinades

The aim of the study was to determine effects of four commercial marinades on the colour, tenderness, cooking loss and sensory characteristics of semimembranosus beef muscles before and after sous-vide (SV) treatment. Muscles (n = 24) were marinated using Odessa (O; red pepper, garlic, onion), Mexico (M; red pepper, tomato), Old Polish (OP; pepper, garlic), and Bordeaux (B; pepper, red pepper, garlic) marinades for 24 h at 4 °C. Marinades uptake ranged from 3.3% (M) to 4.4% (B). Marinating and SV significantly affected all colour parameters. In SV beef, the highest values of L* were noted in OP and O samples, whereas the highest a* and b* values in M samples. Overall, marinating reduced (P < 0.05) cooking loss (34.6%) and shear force (19.5%). The use of marinating prior to SV treatment beneficially affected taste, tenderness and juiciness of beef. OP marinade allowed to obtain beef steaks with the best sensory quality and the lowest shear force.

Evaluation of Moist Beef Jerkys (dendeng lambok) Nutrition as a Specific-Spiced Traditional Food at West Sumatera, Indonesia

Moist beef jerky (dendeng lambok) is one of traditional food in Indonesia that potential to be introduced to the world. This kind of food is a form of diversification of processed beef products that use natural spices as ingredients that can improve the taste and nutritional value of beef. In this study, the nutritional changes of the beef on the production of moist beef jerky as a specific-spiced traditional food at West Sumatera, Indonesia were evaluated. The production of traditional moist beef jerky that treated with some specific spices prior to frying, might induced the nutritional compound of the raw beef therefore the nutritional properties such as proximate contents, amino acids, fatty acids and in-vitro protein digestibility of the raw beef was compared with the spiced-fried beef. The Association of Official Analytical Chemist’s method, Ultra Performance Liquid Chromatography-Photo Diode Array Detection, Gas Chromatography-Flame ionization Detection and in-vitro enzymatic analysis were used to analyze proximate contents, amino acids, fatty acids and in-vitro protein digestibility of the samples respectively. As the result, the moisture content and carbohydrate content of fried beef decreased significantly compared to the raw beef while the protein and fat content increased. Moreover, the frying process of spiced beef could increased the amino acids, fatty acids and in-vitro protein digestibility of the beef. The present study showed that the quality characteristics of moist beef jerky were significantly improved compared to the raw beef especially on protein profile, fat profile and in-vitro protein digestibility. The utilization of specific spices and frying process on the production of moist beef jerky not only contribute to nutritional properties, but also contribute to its taste and flavor. The increment of amino acids and fatty acids might contribute to the emergence of specific aroma and flavor of produced moist beef jerky. Moreover, the specific study on the interaction of moist beef jerky’s spices on its nutritional properties should be clarified further.

Organoleptic properties of pineapple peel juice marinated beef (Ananas comosus L. Merr)

Beef has a high level of toughness that is not liked by consumers. The solution to increasing beef tenderness is to soften the beef. One method for tendering the beef is by marinating using local ingredients that are easily available, abundant, and inexpensive, namely pineapple. Pineapple peel is waste from pineapple that has not been utilized and is usually discarded. In pineapple peel, there is a bromelain enzyme thought to be able to hydrolyse protein so that it can tender beef. The purpose of this study was to find out the organoleptic properties of beef using the marination with various pineapple peel juice concentrations (Ananas comosus L. Merr) namely 0%, 10%, 20%, and 30%. This study used an experimental method with a Completely Randomized Design consisting of 4 treatments and 4 replications. The parameters in this study were organoleptic properties namely colour, aroma, texture, tenderness, and taste. Results of the study showed that beef marination with various pineapple peel juice concentrations had a significant difference (P&lt;0.05) on taste, texture, and tenderness of beef but had no significant difference in colour and aroma (P&gt;0.05)

365 Palatability of Beef Products: A Perspective on Future Expectations

Abstract The number one driver of consumer demand for beef and beef products is taste. The beef industry continuously measures consumer perceptions of beef and performance of beef products against evolving consumer expectations. In order to maintain consumer preference for beef taste attributes, the industry has dedicated years of meat science research to improve its product and measure its progress. This session will share the outcomes of both technical and consumer research focused on beef palatability.

N,N'-Diphenylthiourea electrochemical sensor for the detection of l-glutamate and Aspartate in beef.

This study was conducted to design an electrochemical sensor for detection of l-glutamate (L-Glu) and Aspartate (Asp), which contribute largely to the umami taste of beef. Using N, N'-diphenylthiourea (DPTU), polypyrrole (PPy), and polyvinyl chloride (PVC), a composite electrode (DPTU/PVC/PPy/Pt) was prepared for rapidly electrochemical detection of l-Glu and Asp. The surface morphology of the synthesized functionalized electrode was characterized by Field Emission Scanning Electron Microscopy (FESEM). Potentials of umami amino acids accounted for 97.8%, while six interferential amino acids existed. The linear correlation between the content of l-Glu and Asp in beef broth was studied under different treatment conditions. The sensor compared with an amino acid analyzer well detected the contents of l-Glu and Asp in beef broths, with a coefficient of 0.991 in Pearson correlation analysis and an accuracy of 88.9%. The proposed electrochemical sensor showed good concurrence with previously reported methods and was effectively employed for the quantification of l- Glu and Asp in beef. PRACTICAL APPLICATION: The sensor exhibits the good performance with high stability and high accuracy. And it is a potential sensing platform with good reproducibility, making the proposed method suitable and reliable for routine analysis of L-Glu and Asp in beef. This method was proved promising for quantitative detecting amino acids in beef.