Tartaric Acid Esters Of Monoglycerides Research Articles

Measurement of physicochemical and dough pasting properties of fresh potato instant noodles as influenced by addition of emulsifiers

Effects of different types and concentrations of emulsifiers on physicochemical properties of fresh potato added instant noodles and its dough pasting properties were analyzed. The Rapid visco-analyzer (RVA) data showed that noodle sample at 30 % polysorbate-40 (P40) exhibited significantly high pasting properties compared to control sample and changed slightly at added distilled monoglycerides (DMGs) and diacetyl tartaric acid esters of mono-glycerides (DATEM) levels. Rheological analysis exhibited comparable storage modulus (Gʹ) to loss modulus (Gʹʹ) and tanδ higher than control sample. The fresh potato instant noodles added with DMGs and DATEM exhibited low textural parameters, enhanced color parameters and loose microstructures. Fourier transform infrared spectroscopy (FT-IR) analysis revealed that molecular structure of the noodles was not affected by the added emulsifiers. Low field nuclear magnetic resonance (LF-NMR) analysis indicated that distribution of water fraction in fresh potato noodles was slightly affected by the added emulsifiers.

Effect of glucose oxidase enzyme and diacetyl tartaric acid ester of monoglycerides (DATEM) emulsifier on the physical and textural characteristics of cupcake

Effect of glucose oxidase enzyme and diacetyl tartaric acid ester of monoglycerides (DATEM) emulsifier on the physical and textural characteristics of cupcake

Gluten Conformation at Different Temperatures and Additive Treatments.

The effect of temperature (25, 45, and 65 °C) on the gluten secondary structure was investigated by using Fourier transform infrared (FTIR) spectroscopy and modulation of disulfide and hydrogen bonds contributions (100 ppm ascorbic acid (AA), 0.6% diacetyl tartaric acid ester of monoglycerides (DATEM), and 0.25 mM dithiothreitol (DTT)). The results showed that additives heated at 65 °C altered most of the gluten matrix formation by changing structural secondary structures compared to the secondary structures of native gluten (control). The content of random coils, α-helices, and β-sheet of gluten increased, while the extent of β-turns and antiparallel β-sheets decreased, which led to the transformation to a more stable secondary conformation. In addition, the rheological properties (%creep strain) revealed that gluten deformation increased during the heating process with all of the additives. The chemometric method could quantitate an overall alteration of gluten polymerization and gluten matrix formation during heating with additive treatments.

Influence of diacetyl tartaric acid ester of monoglycerides on the properties of whey powder–maltodextrin emulsion

The aim of this study was to prepare a stable oil-in-water emulsion by whey powder (WP)–maltodextrin (MD) at room temperature for further industrial processing. Due to the weak surface activity of WP and MD, diacetyl tartaric acid ester of monoglycerides (DATEM) was added to the system as an emulsifier and the effect of DATEM concentration on the properties of WP–MD emulsions was investigated. As the concentration of DATEM increased, the mean droplet size, zeta potential, surface tension, apparent viscosity, and morphology of the emulsions changed significantly. Fourier transform infrared analysis showed that the strength of hydrogen bonding in the emulsions was positively correlated with DATEM concentration, and the interaction between DATEM and the protein caused changes in the secondary structure of the protein, which benefited the stability of the emulsions. Furthermore, the experimental results also indicated that the stable WP–MD emulsion could be formulated above the threshold value of DATEM of 0.125%. Novelty impact statement The purpose of this study was to provide a new idea to solve the problem of cheese whey pollution so that whey powder (WP) and maltodextrin (MD) can be used as wall materials for oil-loaded food emulsions and microcapsules, as well as to combine fundamental research with practical production situations to expand their practical application potential. The addition of diacetyl tartaric acid ester of monoglycerides (DATEM) can compensate for the weak surface activity of WP and MD and a stable WP–MD emulsion can be effectively formulated above the threshold value of 0.125% DATEM concentration.

Effects of pectin and emulsifiers on the physical and nutritional qualities and consumer acceptability of wheat composite dough and bread.

Rheological behaviour and certain quality attributes of the dough and bread prepared from the wheat-millet-Bambara flour (WMB) containing mixtures of emulsifiers and/or apple pectin were investigated. WMB was prepared by substituting wheat flour (WF) with 25% millet flour and 25% Bambara flour. Pectin (1.0-2.0g) and emulsifiers namely sodium stearoyl lactylate (0.25-0.40g), polysorbate 80 (0.50-0.80g), and diacetyl tartaric acid ester of monoglycerides (0.10-0.25g) mixed in different proportions were added to produce dough and bread. Mixolab was utilised to measure the rheological behaviour of dough and bread made from all mixes were analysed for physical characteristics, nutritional composition, and organoleptic properties. A significant increase in dough development time (emulsifier: 65% and pectin: 57.9%) and dough stability (emulsifier: 18.2% and pectin: 35.2%) were observed. Loaf volume, specific volume and proximate composition of the composite bread increased significantly relative to control. Protein content (33%), protein digestibility (85%) and certain essential amino acids (lysine: 54.6%; threonine: 36.4%) increased significantly in the WMB bread compared to the WF bread. Sensory evaluation revealed an above average acceptability for the composite bread samples. Emulsifiers and pectin used in the present study resulted in significant improvement in the dough rheology, as well as in the physical characteristics, the nutritional and sensory attributes of the WMB composite bread. The results of the present study confirm the potential for supplementation and fortification of wheat bread using flours from millet and Bambara sources.

Mapping the location of DATEM in multi-phase systems: Synthesis and characterization of spin-label probe analogues

Electron paramagnetic resonance (EPR) spectroscopy has emerged as a fast, reliable, non-invasive and sensitive method to determine the distribution, localization and reactivity of labelled ingredients in micro-heterogeneous systems. However, the commercially available probe molecules are very limited. In the present work, five new nitroxide [(4-hydroxy-2, 2, 6, 6-tetramethylpiperidin-1-oxyl (TEMPOL)] derivatives (1b–5b) of diacetyl tartaric acid esters of monoglycerides (DATEM) (1a–5a), a widely used food emulsifier, were synthesized under Steglich conditions and characterized by MS (mass-spectrometry), FT-IR (Fourier-transform infrared spectroscopy), EPR, NMR (nuclear magnetic resonance spectroscopy), fluorescence spectroscopy, and DSC (differential scanning calorimetry) analysis. Phase partitioning studies proved that the new spin labels are adequately capable of describing the localizations and partitioning of the corresponding DATEM in multi-phase systems. Findings disclosed in this work will provide new knowledge on ingredient reactivity and localization in multi-phase systems; which is vital to aid the design of more efficient delivery systems for bioactive compounds.

Understanding the effect of emulsifiers on bread aeration during breadmaking.

Much research has been done to explain the action of emulsifiers during breadmaking, but there is still plenty unknown to elucidate their functionality despite their diverse chemical structure. The aim of the present study was to provide some light on the role of emulsifiers on air incorporation into the dough and gas bubbles progress during baking and their relationship with bread features. Emulsifiers like diacetyl tartaric acid ester of monoglycerides (DATEM), sodium stearoyl lactylate (SSL), distilled monoglyceride (DMG-45 and DMG-75), lecithin and polyglycerol esters of fatty acids (PGEF) were tested in very hydrated doughs. Emulsifiers increase the maximum dough volume during proofing. Emulsifiers increase the number of bubbles incorporated during mixing, observing higher number of bubbles, particularly with PGEF. Major changes in dough occurred at 70 K when bubble size augmented, becoming more heterogeneous. DMG-75 produced the biggest bubbles. As a consequence, emulsifiers tend to increase the number of gas cells with lower size in the bread crumb, but led to greater crumb firmness, which suggested different interactions between emulsifiers and gluten, affecting protein polymerization during baking. The progress of the bubbles during baking allowed the differentiation of emulsifiers, which could explain their performance in breadmaking. © 2018 Society of Chemical Industry.

The Effects of Egg and Diacetyl Tartaric Acid Esters of Monoglycerides Addition on Storage Stability, Texture, and Sensory Properties of Gluten-Free Sorghum Bread.

The impact of whole egg addition (as is) at 20%, 25%, or 30% (flour basis) on sorghum bread quality was evaluated. The use of the antistaling agent diacetyl tartaric acid esters of monoglycerides (DATEM) at 0.5% (flour basis) at each of the egg addition levels was also studied. Evaluated quality factors included color, specific volume, and crumb structure. Texture analysis was performed to evaluate the rate of quality loss based on changes in crumb hardness values over time. A trained sensory panel evaluated bread quality attributes by descriptive analysis. Sorghum breads with egg had larger specific volumes than the control, while DATEM had a negative effect on the volume of sorghum gluten-free bread. Inclusion of egg in the bread formula improved cell structure and produced darker crust (P < 0.05). The addition of egg reduced bread hardness and slowed the rate of quality loss over a 12-d storage period. Descriptive analysis confirmed the findings of texture analysis. Control breads were significantly harder (P < 0.05) than egg-containing bread at days 0 and 4. This research demonstrates that addition of eggs to a gluten-free sorghum bread formulation results in improved storage stability and better overall quality and acceptability of the product.

Functionality of Different Surfactants on the Rheological and Micro‐Structural Characteristics of Multigrain Premix Incorporated Wheat Flour

AbstractEffect of different surfactants namely, lecithin, glycerol monostearate (GMS), sodium stearoyl‐2 lactylate (SSL) and di‐acetyl tartaric acid esters of monoglycerides (DATEM) at three different levels (0.25, 0.5 and 1%) on the rheological and micro‐structural characteristics of multigrain premix incorporated wheat flour (MWF) were studied. Surfactants significantly (P ≤ 0.05) increased the consistograph water absorption capacity, tolerance, alveograph average abscissa at rupture and mixogram peak value of MWF and decreased the consistograph dough development time, alveograph tenacity, mixograph height and width. DATEM and lecithin decreased the rapid visco analyzer peak viscosity of MWF, whereas GMS and SSL increased the same. The maximum increase in tolerance and peak viscosity were observed with GMS and SSL at 0.5% level when compared to other surfactants at the same level. SEM studies showed thick protein matrix with 0.5% of SSL and DATEM, whereas with GMS, the protein matrix appeared smooth, uniform and continuous. Among the surfactants studied, GMS and SSL at 0.5% level showed maximum effect on the rheological and micro‐structural characteristics of MWF.Practical ApplicationsRheological characteristics of wheat flour are effective in predicting the behavior of the dough during processing and the quality of end product. Incorporating multigrain premix to wheat flour adversely affected the rheological characteristics of wheat flour. This article discusses the improvement effect of different surfactants on the rheological and micro‐structural characteristics of multigrain premix incorporated wheat flour. This article also suggests the better surfactant for multigrain incorporated wheat flour to improve its rheological characteristics. Knowledge of functionality of different surfactants on multigrain incorporated wheat flour will be very much useful for the industries to develop nutritious multigrain products with desired quality using surfactants.

استفاده از خواص مکانیکی خمیر در پیشگویی رئولوژی خمیر، جنبههای کیفی، حسی، ماندگاری و ریزساختار نان: بررسی با روشهای چند متغیره متقارن و نامتقارن

خواص مکانیکی خمیر نقش مهمی در صنایع پخت ایفا می‌کنند و در مواردی از قبیل تثبیت افزودنی‌ها و مطالعه تاثیر آنها بر سایر خواص خمیر و نان می‌توانند مورد توجه واقع شوند. در این پژوهش، از روش‌های چندمتغیره متقارن (تعیین ضرایب همبستگی و آنالیز مولفه‌های اصلی) و نامتقارن (سطح پاسخ و رگرسیون حداقل مربعات جزئی) به‌منظور مطالعه تاثیر اجزاء ژل بهبوددهنده بر خواص مکانیکی خمیر نان بربری، ارتباط خواص مکانیکی خمیر با سایر خواص خمیر و نان و نیز بهینه‌سازی فرمول ژل استفاده گردید. نمونه‌های ژل بهبوددهنده، محتوی 0 تا g100/g 5/0 سدیم استئاروئیل لاکتیلات، داتم و پروپیلن گلیکول بودند. نتایج نشان داد که با توجه به تغییر مقدار و نوع اجزاء فرمولاسیون ژل، دامنه وسیعی از تغییرات در خواص مکانیکی خمیر ایجاد می‌شود و از اختلاط g100/g 5/0 سدیم استئاروئیل لاکتیلات، g100/g 5/0 داتم و g100/g 5/0 پروپیلن گلیکول بهترین حالت حاصل می‌گردد. ضرایب همبستگی بین خواص مکانیکی خمیر با خصوصیات فارینوگرافی خمیر، کیفیت، جنبه‌های حسی، پردازش تصویر و ماندگاری نان بررسی گردید. همچنین آنالیز مولفه‌های اصلی ثابت نمود که قادر به استخراج اطلاعات مناسبی است و می‌تواند به‌عنوان یک روش آسان برای تحلیل و تفسیر خواص مکانیکی خمیر و ارتباط آنها با سایر خواص خمیر و نان مورد استفاده قرار گیرد. مدل‌های رگرسیونی حداقل مربعات جزئی نیز برای تعیین ارتباط بین خصوصیات مکانیکی خمیر با خصوصیات فارینوگرافی خمیر، خصوصیات کیفی، حسی، پردازش تصویر و ماندگاری نان به‌کار برده شد. نتایج حاصل از آنالیزهای آماری نشان داد که خصوصیات مکانیکی خمیر، قادر به پیشگویی حداقل 50% از خصوصیات خمیر و نان هستند.

Effect of hydrocolloids and emulsifiers on the shelf-life of composite cassava-maize-wheat bread after storage.

The objective of this study was to evaluate the effect of hydrocolloids and/or emulsifiers on the shelf‐life of composite cassava‐maize‐wheat (ratio 40:10:50) reference bread during storage. Added hydrocolloids were carboxymethylcellulose (CMC) and high methoxyl pectin (HM pectin) at a 3% level (w/w) and/or the emulsifiers diacetyl tartaric acid esters of monoglycerides (DATEM), lecithin (LC), and monoglycerides (MG) at a 0.3% level (w/w). After 4 days of storage, composite breads with MG had comparatively lower crumb moisture while crumb density was similar in all breads. The reference bread crumb firmness was 33.4 N, which was reduced with an addition of DATEM (23.0 N), MG (29.8 N), CMC (24.6 N) or HM pectin (22.4 N). However, the CMC/DATEM, CMC/LC, and HM pectin/DATEM combinations further reduced crumb firmness to <20.0 N. The melting peak temperature was increased from 52 C to between 53.0 C and 57.0 C with added hydrocolloids and/or emulsifiers. The melting enthalpy of the retrograded amylopectin was lower in composite bread with hydrocolloids and emulsifiers, 6.7–11.0 J/g compared to 20.0 J/g for the reference bread. These results show that emulsifiers in combination with hydrocolloids can improve the quality and extend the shelf‐life of composite cassava‐maize‐wheat breads.

Influence of bread improvers on rheological properties of dough supplemented with sweet-potato flour and optimization analysis of complex bread improvers on sweet potato bread quality

In order to improve the quality of bread supplemented with 25% sweet- potato flour,bread improvers were commonly used,such as wheat gluten,emulsifieres,hydrocolloids,enzymes and VC.Effects of different kinds of bread improvers on rheofermentation properties as well as farinogram properties were studied.Results showed that in single factor experiment,the addition of gluten had certainly improved dough fermentation characteristics,and the additions of 4.5% and 5.5% had the most obvious improvement. For other modifiers,emulsifier- diacetyl tartaric acid ester of monoglycerides( DATEM),sodium stearoyl lactic acid( SSL),hydroxypropyl cellulose( HPC),carrageenan,enzymes- glucose oxidase( GOX),xylanase( XYL),lipase( LYP) and ascorbic acid( VC) had different effects on dough fermentation,but hydroxypropyl cellulose( HPC) and lipase( LYP) had no effect on farinogram properties. Using ratio test and response surface design,treating bread hardness and specific volume as indicators,this experiment determined the formula of the compound modifier: compound emulsifieres 0.47%,carrageenan 0.58%,compound enzymes 0.0221%,VC0.00225%,which the theory of specific volume and hardness were 5.01 cm3/ g and 166.3 g,respectively.To test the accuracy of the model,the validation experiment was done under the optimum condition. Bread specific volume was 5.08 cm3/ g and hardness was 171.5 g,which both significantly higher than the control group of specific volume 4.02 cm3/ g and hardness 271.1 g.

Stability of whippable oil-in-water emulsions: Effect of monoglycerides on crystallization of palm kernel oil

The effect of selected monoglycerides on droplet morphology and rheology of palm kernel oil emulsions was studied. Combination of lactic acid ester of monoglycerides (LACTEM) and unsaturated monoglycerides (GMU) yielded highly viscous emulsions caused by partial coalescence. LACTEM combined with saturated monoglycerides (GMS) or diacetyl tartaric acid ester of monoglycerides (DATEM) gave low-viscous emulsions. The rheological behavior was found not to be related to polymorphic transformations of fat in the emulsions.A possible relationship between structural changes of emulsions and the effect of monoglyceride crystallization behavior of PKO was examined by studying the monoglycerides' influence on crystallization of bulk PKO. Polymorphic transformation, crystallization kinetics and melting/crystallization profile of bulk PKO were analyzed. The monoglycerides had minor effect on crystallization behavior of bulk PKO compared to the major impact on emulsion stability. This indicates that emulsifiers act differently in bulk and dispersed fat. For PKO the structural changes of the emulsions were independent of the effects of monoglycerides on the crystallization of bulk fat.

Rheological Characteristics of Gluten after Modified by DATEM, Ascorbic Acid, Urea and DTT Using Creep-Recovery Test

The effects of diacetyl tartaric acid ester of monoglycerides (DATEM), ascorbic acid (AA), urea, and dithiothreitol (DTT) on viscoelastic properties of commercial hard red winter wheat gluten were investigated. A constant shear stress of 40 Pa was applied to gluten during creep-recovery test. Experimental creep-recovery compliance responses were fitted into a Burgers model with four elements accounting for characteristics of pure elastic (spring), viscoelastic (spring-dashpots elements), and viscous flow (dashpot). DATEM decreased the elasticity and viscoelasticity, but increased viscosity of gluten. The addition of AA, urea, and DTT, resulted in opposite rheological properties when compared with DATEM. Relationship among physical properties was also studied with principal component analysis (PCA) including gluten viscoelasticity, dough mixing and baking properties. Regressed coefficients from Burgers model accounted for higher percent of explained variance and were independent from flour content, baking and dough mixing properties.

Effect of emulsifiers on complexation and functional properties of oxidized white sorghum (Sorghum bicolor) starch

The effect of addition of three commonly used emulsifiers namely GMS (glycerol monostearate), SSL (sodium stearoyl lactylate) and DATEM (diacetyl tartaric acid esters of monoglycerides) on complexation, thermal, pasting and textural properties of OWSS (oxidized white sorghum starch) was studied. The study is of interest as both oxidized starches and emulsifiers are present as co-ingredients in different food products and thus their complexation could affect the textural characteristics of foods. The complexation index (CI) reduced on oxidation of sorghum starch. The CI for native white sorghum starch (NWSS) was in the order GMS > SSL > DATEM whereas for OWSS, CI was in the order GMS > SSL > DATEM. Presence of emulsifiers significantly reduced gelatinization enthalpies of starches. Types I and II amylose–lipid complexes were observed in NWSS and OWSS on addition of GMS. Pasting temperature of NWSS increased while peak viscosity reduced on addition of SSL and GMS. Cold paste and setback viscosities of OWSS increased significantly (p ≤ 0.05) on addition of emulsifiers. Emulsifiers reduced firmness and rupture strength of NWSS and OWSS gels. Increase in firmness of gels on storage increased in NWSS and decreased in OWSS on addition of emulsifiers. Elasticity of OWSS on cold storage was higher in the presence of SSL and GMS.

Wheat Varietal Flours: Influence of Pectin and DATEM on Dough and Bread Quality

The effect of two additives, high methoxyl pectin (P) and the emulsifier diacetyl tartaric acid esters of monoglycerides commonly named DATEM (D) and their mixture (P+D) on dough properties and baking performance of two varietal Argentinean wheat flours (‘Buck Pronto’ [BP]; ‘Klein Escudo’ [KE] was analyzed. Rheological characterization of dough (alveogram, farinograms, texture profile analysis-TPA, and rheometric assays), with and without additives, was performed. SEM was used to evaluate the microstructure of dough. Baking performance was analysed by bread volume measurements, shape ratio of loaves (width/height), and the hardness of crumb and crust. Assays on dough showed differences in alveographic force (W) and in most of the texture profile analysis parameters. Assays on bread showed that BP specific volume was improved with the addition of P and P+D, but shape ratio was only improved with the mixture of P+D. Breads from KE flour with additives presented, in all cases, showed higher volumes and a better shape ratio than those obtained with the control sample. Hardness of KE crumb was diminished by all additives but BP crumb was softened only with the addition of P and P+D. All sensory parameters were improved for both types of bread, particularly with D and P+D.

Changes in secondary structure of gluten proteins due to emulsifiers

Changes in the secondary structure of gluten proteins due to emulsifiers were analyzed by Raman Spectroscopy. The protein folding induced by 0.25% SSL (Sodium Stearoyl Lactylate) (GS0.25, Gluten+0.25% SSL) included an increase in α-helix conformation and a decrease in β-sheet, turns and random coil. The same behavior, although in a less degree, was observed for 0.5% gluten–DATEM (Diacetyl Tartaric Acid Esters of Monoglycerides) system. The low burial of Tryptophan residues to a more hydrophobic environment and the low percentage area of the C–H stretching band for GS0.25 (Gluten+0.25% SSL), could be related to the increased in α-helix conformation. This behavior was also confirmed by changes in stretching vibrational modes of disulfide bridges (S–S) and the low exposure of Tyrosine residues. High levels of SSL (0.5% and 1.0%) and DATEM (1.0%) led to more disordered protein structures, with different gluten networks. SSL (1.0%) formed a more disordered and opened gluten matrix than DATEM, the last one being laminar and homogeneous.

Incorporation of several additives into gluten free breads: Effect on dough properties and bread quality

The objective of this work was to assess the effect of emulsifiers, hydrocolloids and enzymes on gluten-free dough rheology and thermal properties and bread quality, while relating dough properties parameters to bread technological quality. Breads were based on rice flour, cassava starch and full-fat active soy flour, with 65% or 75% (flour-starch basis) of water incorporation. Additives used were emulsifiers (diacetyl tartaric acid ester of monoglycerides – DATEM and sodium stearoyl lactylate – SSL), enzymes (glucose oxidase and α-amylase) and hydrocolloids (xanthan gum, carboxymethylcellulose, alginate and carrageenan). Results showed that additive incorporation modified dough behavior, evidenced by different calorimetric and rheological properties. Besides, the electrophoretic pattern of dough extracted proteins changed with glucose oxidase addition. These modifications resulted in breads with different characteristics, such as specific volume, firmness and firming rate, and crumb structure. Nonetheless, they did not necessarily show better quality parameters than the control bread. The control dough displayed good performance for obtaining gluten-free breads of acceptable volume, crumb structure and, principally, with lower hardening rate during storage. Contrary to widespread opinion, this work shows that the presence of additives is not essential for gluten-free bread production. This fact provides new perspectives to the gluten free market at the moment of selecting raw materials and technological parameters, reducing production costs and facilitating gluten free products development.

Emulsifiers: Effects on Quality of Fibre-Enriched Wheat Bread

Resistant starch (RS) is a nutritional ingredient commonly used in bread products as dietary fibre (DF). This ingredient presents similar physiological functions than those imparted by DF, promoting beneficial effects such as the reduction of cholesterol and/or glucose levels on blood. Quality improvement of bread containing RS, with an optimized combination of emulsifiers, will be useful in the development of new and healthy bakery products. The objective of this research was to analyse the effects of different emulsifiers on several quality parameters of dough and bread prepared with wheat flour partially substituted with resistant starch as a dietary fibre. A blend of wheat flour/maize-resistant starch (MRS; 87.5:12.5) with sodium chloride, ascorbic acid, α-amylase, compressed yeast and water was utilized. Emulsifiers were incorporated to formulations in different levels according to a simplex centroid design. The viscoelastic, textural and extensional properties of dough were analysed. Bread quality was evaluated throughout the gelatinization and retrogradation of starch, specific volume of loaves, and texture and firmness of bread crumb. The incorporation of 12.5% (w/w) of MRS to wheat flour caused an increase of 5% in water absorption. Stability decreases markedly (from 9.9 to 2.2 min) and the mixing tolerance index increased (from 79 to 35 UF). The sodium stearoyl lactylate (SSL)–diacetyl tartaric acid esters of monoglycerides (DATEM) mixture increased hardness and resistance to extension on dough, whilst dough containing Polysorbate 80 (PS80) was softer; nevertheless, both types of dough retained less CO2. An optimized concentration of the three emulsifiers (0.24% SSL, 0.18% PS80, 0.08% DATEM, w/w) was obtained by surface response methodology. The bread prepared with this combination of emulsifiers presented a considerable specific volume with a very soft crumb.

Analysis of soluble proteins/aggregates derived from gluten-emulsifiers systems

According to their amphiphilic nature, emulsifiers as Sodium Stearoyl Lactylate (SSL) and Diacetyl Tartaric Acid Esters of Monoglycerides (DATEM) favor interactions between dough components, although the mechanisms of action are not fully elucidated. There is quite information about the nature of emulsifier–gluten protein interaction and its consequence in breadmaking quality, but no reports were found about structural changes in gluten proteins produced by SSL and DATEM and their influence in aggregation–disaggregation gluten protein phenomena. For this reason the aim of this work was to investigate changes on gluten protein structure induced by SSL and DATEM; through the analysis of the nature of soluble aggregates by different SDS-PAGE techniques, and gliadins and glutenins extracted from gluten samples by RP-HPLC. At 1% level, SSL allowed the solubilization of a large proportion of high molecular mass aggregates, suggesting that at this high level, this emulsifier cause changes in native gluten protein network. Nevertheless, no significant differences in the protein quantity extracted of the distinct gliadins and glutenins were observed. On the other hand, the emulsifier SSL at 0.5% (w/w) level also allowed the extraction of a high percentage of γ-gliadins and low molecular weight glutenins in comparison with gluten and gluten-DATEM samples. In conclusion distinct quantity and quality of gliadins and glutenins were extracted from gluten samples, depending on the level and the chemical structure of each emulsifier.