Aim: The present study was conducted to evaluate the relationship between pulp weight and various physio-biochemical attributes among 30 diverse mango (Mangifera indica L.) germplasms under the Tarai region of Uttarakhand. Place and Duration of Study: This investigation was carried out at Horticulture Research Centre, GB Pant University of Agriculture and Technology, Pantnagar, Uttarakhand, during the years 2021 and 2022. Methodology: The selected 30 germplasms included two commercially grown cultivars and 28 primary germplasms sourced from different eco-geographical locations of Uttar Pradesh and Uttarakhand states of India. Pulp weight, physical characteristics (fruit shape index, pulp/stone ratio) and biochemical parameters (Brix/Acid ratio and pH) were measured for each germplasm and statistically laid out in completely randomized design (CRD), and their relationships were analyzed using simple linear regression and Pearson’s correlation coefficients. Results: The maximum values recorded among the studied germplasms were 300.73 g for pulp weight (PMGC-19), 2.41 for fruit shape index (PMSS-31), 0.309 for pulp/stone ratio (PMGC-71), 140.87 for Brix/Acid ratio (PMGC-96) and 5.87 for pH (PMGC-96). Among the physical attributes, pulp weight exhibited a moderate positive correlation with fruit shape index (r = 0.419), indicating that fruits with a higher shape index tend to have increased pulp weight. Whereas, a moderate negative correlation was observed between pulp weight and pulp/stone ratio (r = -0.457), suggesting that as the pulp/stone ratio increases, pulp weight decreases. However, biochemical attributes such as Brix/Acid ratio and pH showed no significant correlation with pulp weight (r = 0.005 and r = 0.018, respectively), indicating an independent influence of these parameters on pulp characteristics. Conclusion: These findings provide important insights into the complex relationships between pulp weight and other physio-biochemical traits in mango, some traits for breeding programs aimed to improving fruit quality and pulp content and identified superior and promising germplasms for cultivation as well as potential parental genotypes in mango improvement programs.