This study aimed to determine the results of total tannin content determination of sapodilla peel extract (Manilkara zapota) and the effectiveness of sapodilla peel extract (Manilkara zapota) as a Aedes aegypti mosquito larvicide. The method used was a quantitative descriptive with a purposive sampling method. The sample was ripe sapodilla (Manilkara zapota) peel. The determination of tannin content in sample extracts was analyzed using UV-Vis spectrophotometry. The extraction of tannins from sapodilla peel (Manilkara zapota) was carried out by maceration method using 70 % ethanol. Based on the research results it was found that the total content of sapodilla peel extract was 3,058 mg GAE/g extract. The results of the larvicide test showed that the sapodilla peel extract could be used as an Aedes aegypti larvicide with the most effective concentration in killing 50% of the larvae at a concentration of 20,000 ppm.