Parasitic plants have been viewed as pests since they are able to damage agricultural crops and forest trees. They establish vasculature connections with the hosts and withdraw the required nutritive resources. This study aimed to explore the physiological and biochemical effects of the parasitic plant Plicosepalus acacia on the host Tamarix aphylla. It was conducted on young fully expanded leaves from the uninfected and infected trees and the parasitic plant ‘in situ’. The parasite had higher net photosynthetic assimilation rate (A), transpiration rate (E) and stomatal conductance (gs) compared to the host. Equally, it had two-fold greater water potential (Ψ) and osmotic potential (Ψs). It accumulated high amount of K, while it avoided accumulation of the most trace and ultratrace elements. Otherwise, parasitism seemed to increase A, WUE, water uptake and accumulation of the most major, trace and ultra-trace elements, however it reduced the accumulation of osmolytes at the infected plants. Based on UPLC-MS approach, P. acacia seemed to use a group of composites to interact with the host, including oleamide as a protector metabolite against host’s defense system, carvone to establish vasculature connections with the host, cuminaldehyde to weaken growth and proliferation of the host, and caprolactam to weaken the distal part of the host. In contrast, the host T. aphylla could be used pipecolinic acid and nicotinamide to regulate systemic resistance and to defense against the parasite infection. Finally, despite the defense molecular interactions between the two partners, the parasitic plant exhibited several beneficial effects on the host.