While the resting metabolic rate (RMR) is crucial for understanding athletes' energy requirements, limited information is available on the RMR of Paralympic athletes. To determine RMR and its predictors in a diverse cohort of Paralympic athletes and evaluate the agreement between measured and predicted RMR from both newly developed and preexisting equations. This cross-sectional study, conducted between September 2020 and September 2022 in the Netherlands and Norway, assessed RMR in Paralympic athletes by ventilated hood indirect calorimetry and body composition by dual energy X-ray absorptiometry. Sixty-seven Paralympic athletes (male: n=37; female: n=30) competing in various sports, with a spinal cord disorder (SCD; n=22), neurological condition (n=8), limb deficiency (n=18), visual or hearing impairment (n=7) or other disability (n=12) participated. RMR, fat-free mass (FFM), body mass, and triiodothyronine (T3) concentrations were assessed. Multiple regression analyses were conducted with height, FFM, body mass, sex, T3 concentration, and disabilities as potential predictors of RMR. Differences between measured and predicted RMRs were analyzed for individual accuracy, root mean square error (RMSE), and intraclass correlation (ICC). Mean RMR was 1386±258 kcal/day for females and 1686±302 kcal/day for males. Regression analysis identified FFM, T3 concentrations and the presence of a spinal cord disorder (SCD), as the main predictors of RMR (adjusted R2=0.71; F=50.3; P<0.001). The novel prediction equations based on these data, as well as pre-existing equations of Chun et al. and Nightingale and Gorgey performed well on accuracy (>60% of participants within 10% of measured RMR), had a good reliability (ICC >0.78), and low RMSE (≤141 kcal). FFM, total T3 concentrations, and the presence of SCD are the main predictors of RMR in Paralympic athletes. Both the current study's prediction equations and those by Chun et al. and Nightingale and Gorgey align well with measured RMR, offering accurate prediction equations for the RMR of Paralympic athletes.