Women’s access to critical resources such as land is one of the most topical issues amongst development practitioners, gender advocates, and policymakers alike. Land is one of the bases of wealth, power, and authority in most developing countries. As such, no meaningful development takes place without land resources, yet gender inequalities concerning land allocation and distribution exist in most Sub-Saharan African countries, especially northern Ghana where the majority of the affected are women. This paper examined the effect of the customary land tenure system on landless women smallholder farmers and Agro-processors in West Mamprusi Municipality. Primary data from individuals and household heads, chiefs, and Tingban-dima (landowners), were gathered using semi-structured interviews, focus group discussions, and in-depth interviews. The secondary was also extensively reviewed as a complement to the primary data in the area of study. The study revealed significant gender disparities between men and women relative to land allocation and land access. Women's access to land was abysmal compared to that of men. The situation was also found to be aggravated by the dominant patriarchal relations which favor men relative to property ownership. only a handful of women had owned and had access to some lands but in most cases, such lands were reported to be poorer in quality, and lack proximity relative to complementary resources such as water. additionally, some lands in the hands of women came in with hefty royalties, thereby exacerbating their plight. The productive capacity of women in food production, agro-processing, vegetable production, and animal husbandry was found to be impeded by the land tenure system which limits their livelihood opportunities thereby widening the poverty gap between women and men. Nonetheless, women's access to land has seen a bit of improvement over the years, considering the recognition of women’s contribution to household income and the nutritive diet of children, but Indigenous traditional inheritance structures still limit the potential of women in this direction. The need to therefore advocate for a change in traditional land ownership and distribution by emphasizing on women’s rights and contribution to development. The constitution of Ghana allows for equal access to land irrespective of gender; this must therefore be advocated. This will address the gender gap in land and water resources to pave the way for women in agriculture, which will improve rural livelihoods, especially women of reproductive age.