In order to break the conflict between family and work, lack of work self-efficacy, and even fall into the dilemma of job burnout, this paper, from the perspective of gender mainstreaming, deeply analyzes the reasons why the development of female leaders in colleges and universities falls into the dilemma. The development of female leaders in colleges and universities lacks the support of social culture, and there are institutional obstacles such as the imperfection of organizational assessment mechanism. Therefore, this study proposes a more structured and systematic development strategy. On the one hand, the organization should actively promote the establishment of female leadership assessment and evaluation management mechanism embedded in the social gender mainstreaming, and accelerate the socialization process of housework. On the other hand, it is necessary to strengthen the cognition of female leaders in colleges and universities so as to obtain good to great leadership, in a number of measures to promote the role change of female leaders in colleges and universities, highlight the role in social development, truly release the “gender dividend” in colleges and universities.