For many years, plants have been utilized in food, healing materials, and curing for many illnesses. Lately, improvements in biological searches have displayed the notable potential of natural compounds.Objective: In this study, biological activities of extracts of herbal mixtures with propolis were investigated.Materials and Methodology: Ethanol and hexane extracts of propolis-Syzygium aromaticum mixture, propolis-Papaver somniferum mixture, propolis-Foeniculum sp. mixture were used in the assays.Results: Ethanol extracts exhibited higher antibacterial activity compared to hexane extracts. While ethanol extracts inhibited bacterial growth ranges from 7±1.41 mm to 19.5±2.12 mm, hexane extracts showed inhibition zones ranges from 7±0.00 mm to 14±1.41 mm. The maximum and the minimum total phenolic contents were detected in propolis-S. aromaticum ethanol extracts as 389.81±0,001 μg GAE/mL and in propolis-Foeniculum sp. as 100.57±0.012 μg GAE/mL, respectively.Conclusion: Studied plant extracts with propolis might be an option to synthetic antioxidant and antibacterial compounds.