Synthesis Of Magnesium Oxide Research Articles

Effect of Green Synthesized Magnesium Oxide Nanoparticles Using Crataegus aronia L. on Human Colon Cancer – CaCo-2 Cell Line

Effect of Green Synthesized Magnesium Oxide Nanoparticles Using Crataegus aronia L. on Human Colon Cancer – CaCo-2 Cell Line

IMPROVING COWPEA (Vigna unguiculata (L.) Walp.) YIELD WITH GREEN SYNTHESIZED MGO NANOPARTICLES USING Jatropha tajonensis LEAF EXTRACT

The aim of this work was to investigate the effect of synthesized magnesium oxide nanoparticles of Jatropha tajonensis leaf extract on the growth and yield of cowpea (Vigna unguiculata (L.) Walp.). Materials and Methods. The preparation and planting of the cowpea seeds; The extraction of extract of Jatropha tajonensis leaves in aqueous solution. The synthesis of MgO nanoparticles from the extract, followed by characterization to confirm the formation — UV-VIS, FTIR, SEM-EDX and PXRD. The effects of MgONPs on cowpea (Vigna unguiculata (L.) Walp.) plants were surveyed under field conditions to assess its uses in improving growth and yield of cowpea. Results. The results showed that different doses of MgONPs applied to cowpea plant significantly affected all measured parameters of cowpea plantlets under the field condition in a positive way. The best results in growth, yield and the phonological parameters were cowpea plants treated with high MgONP applications (100 mg/L). It has been observed that different MgONPs applications have significant effects on vegetative growth and yield parameters of cowpea. A significant increase in the number of vegetative parameters was observed in the pots with different doses of nano-20, 40, 60, 80 and 100/MgONPs applications compared to the control. Different MgO (with or without NPs) treatments led to significant differences in shoot formation (P < 0.01). According to the effect of different doses of magnesium NPs applied to the cowpea, plant height varied between 18.88 ± 2.51 and 21.35 ± 3.25. The highest value in the height was obtained from nano-100 mg/L MgONPs application with 21.35 ± 3.25 and the lowest value was obtained from the salt 17.48 ± 3.83 mg/L MgONPs application. Conclusion. This study found that MgONPs greatly influenced the plantlets’ growth parameters and other measured traits; in addition. There was an indication that the efficiency of growth and yield of cowpea could be improved by increased application of MgO in the form of nanoparticles. Also, highlighted was the possibility of using MgONPs in increasing another crop yield to cater for the evergrowing world population.

Photocatalytic degradation of acid violet dye by sunlight exposure using green synthesized magnesium oxide nanoparticles

An aqueous extract of Phyllanthus Emblica fruit extract was used as a precursor in the effective synthesis of Magnesium Oxide Nanoparticles (MgONP) and the same was calcined at different temperatures. The thermal study demonstrated that MgO nanoparticles evolved from Mg(OH)2 by the identification of characteristic weight loss curves and endothermic peaks in TGA and DTA, respectively. UV-visible analysis showed that the peak shifts to a longer wavelength at 350 nm when the calcination temperature rises from 200 to 600 °C, indicating a decrease in the size of MgONP particles. On the other hand, Tauc plot analysis showed that the band gaps between the precursor and calcined MgO samples varied from 5.84 to 3.70 eV. FTIR and XRD analysis confirmed the synthesis of MgONPs from Mg(OH)2 exhibiting spherical shapes of around 20 nm in size.Twenty minutes of exposure to sunshine caused the dye's colour to entirely vanish, demonstrating the remarkable photocatalytic effectiveness of MgONP calcined at 600 °C. The effective breakdown of Acid Violet was confirmed by the rate constant of the photocatalytic behaviour increasing proportionately with the catalyst dose. Furthermore, it was suggested by the Langmuir and Freundlich isotherm models that Acid Violet efficiently adheres to the photocatalyst surface when R2 values are nearer to 1. There are several potential applications for MgO nano adsorbents in dye removal processes, as evidenced by desorption studies that showed their reusability.

Nanoparticles (MgO) utilising Justicia adhatoda and an evaluation of their antimicrobial properties

Researchers are using safer green synthesis methods to make nanoparticles. Industry uses green synthetic methods more than physicochemical methods. Green MgO nanoparticle production is environmentally friendly, non-toxic, stable, and offers many nanoscale production options. Green synthesis methods can produce large amounts of nanoparticles, but there are still challenges. The biological extracts during synthesis prevent reaction mechanisms from being understood. Justicia adhatoda plant leaves were employed as a natural ligation agent to generate magnesium oxide nanoparticles. The functional group analysis was done with Fourier Transform Infra-Red spectroscopy. The green synthesized magnesium oxide NPs were crystalline and spherical, as shown by X-ray diffraction and scanning electron microscopy. Analytical investigations determined that particles are 22.96 nm in size. The antimicrobial studies were carried for the Justicia adhatoda MgO nanoparticles against few gram-positive and gram-negative bacteria and fungi. The binding effect was greater at higher concentrations than the control Gentamicin.

Biosynthesis of Magnesium Oxide Nanoparticles using <i>Pennisetum Purpureum</i> and Application in Removal of Cadmium Contaminant from Water

This study evaluated an innovative approach to synthesize magnesium oxide nanoparticles (MgO NPs) through a green synthesis method using Pennisetum purpureum extract as a reducing and stabilizing agent. The green synthesis of MgO NPs is cost-effective and environmentally friendly. Characterization techniques such as X-ray diffraction (XRD), scanning electron microscopy (SEM), and Fourier- transform infrared spectroscopy (FTIR) confirmed the successful synthesis of nanoparticles with unique properties. Debye-Scherrer equation is used for the determination of particle size, which was found to be 15.7nm. The potential application of MgO NPs in the removal of cadmium (Cd) contaminant from water sources was investigated through batch adsorption experiments. Cadmium pollution poses a severe threat to ecosystems and human health due to its toxic nature and persistence in the environment. MgO NPs, with their high surface area and reactivity, efficiently adsorbed and immobilize cadmium ions from water. Adsorption isotherm models indicated that Langmuir isotherm is the best model to describe the adsorption of Cd2+ on magnesium oxide nanoparticles prepared by elephant grass extracts. It was found that a removal of greater percent of Cd2+ could be accomplished through adsorption with 0.05g of magnesium oxide nanoparticles, an initial concentration of metal at 5ppm pH of 10 and at contact time of 65minutes.

Fabrication and evaluation of Dillenia indica-carrageenan blend hybrid superporous hydrogel reinforced with green synthesized MgO nanoparticles as an effective wound dressing material

An unexplored hybrid superporous hydrogel (MHSPH) of Dillenia indica fruit mucilage (DIFM) and carrageenan blend embedded with green synthesized magnesium oxide nanoparticles (MNPs) is utilized as an effective wound dressing material with appreciable mechanical strength in murine model. The prepared MNPs and the optimized MHSPH were characterized using X-ray diffraction (XRD), thermogravimetric analysis (TGA), Fourier transform infrared (FT- IR) spectroscopy. Size, zeta potential and morphology of MNPs was assessed using Dynamic light scattering technique (DLS) and field-emission scanning electron microscopy (FESEM) respectively. The MHSPH grades were further optimized using swelling study in phosphate buffer solution at pH 1.2, 7.0, and 8. Both MNPs and the optimized grade of MHSPH were evaluated based on hemolysis assay, and protein denaturation assays indicating them to be safe for biological use. Acute toxicity studies of the optimized MHSPH on Zebra fish model, revealed no observable toxic effect on the gill cells. Wound healing in Swiss albino mice with application of optimized grade of MHSPH took only 11 days for healing when compared to control mice where healing took 14 days, thus concluding that MHSPH as an effective dressing material as well as tissue regrowth scaffold.

Effective removal of Cr(VI) and Pb(II) ions from mining wastewater using eco-friendly synthesized magnesium oxide nanoparticles incorporated rice husk ash

This study explored the effective adsorptive performance of magnesium oxide (MgO) nanoparticles incorporated rice husk ash (RHA) to address the removal of Chromium (VI) and Lead (II) from mining wastewater from aqueous environment. MgO nanoparticles were synthesized using a magnesium salt precursor and moringa oleifera leaf extract and then impregnated onto RHA to create MgO-RHA nano composite. Different analytical techniques were employed to characterize the RHA and MgO-RHA nanocomposites. The prepared adsorbents were used in batch adsorptive studies and the concentrations of the metal ions were measured before and after treatment using atomic absorption spectrophotometry. Morphological analysis confirmed that the adsorbents developed exhibited a dispersed and porous nature. BET analysis revealed that RHA, MgO, and MgO-RHA had surface areas of 93.04, 32.02, and 102.71 m2/g, respectively. MgO-RHA outperforms RHA in removing metal ions due to its increased surface area and functionality. MgO-RHA demonstrated higher removal percentages of up to 79.20 % and 96.02 % for Cr(VI) and Pb(II), respectively. The Pseudo-second order kinetics and Langmuir isotherm demonstrated the highest degree of conformity to the experimental data, considering the coefficient of determination (R2), smallest value of SSE and chi-square (ᵪ2). Thermodynamic results indicated that the adsorption process was characterized by endothermicity, spontaneity and chemisorption controlled. In summary, the performance of the MgO-RHA nanocomposite surpassed that of RHA alone, which serves as a better adsorbent for metal ion removal.

Magnesium Oxide (MgO) Nanoparticles: Synthetic Strategies and Biomedical Applications

In recent times, there has been considerable interest among researchers in magnesium oxide (MgO) nanoparticles, due to their excellent biocompatibility, stability, and diverse biomedical uses, such as antimicrobial, antioxidant, anticancer, and antidiabetic properties, as well as tissue engineering, bioimaging, and drug delivery applications. Consequently, the escalating utilization of magnesium oxide nanoparticles in medical contexts necessitates the in-depth exploration of these nanoparticles. Notably, existing literature lacks a comprehensive review of magnesium oxide nanoparticles’ synthesis methods, detailed biomedical applications with mechanisms, and toxicity assessments. Thus, this review aims to bridge this gap by furnishing a comprehensive insight into various synthetic approaches for the development of MgO nanoparticles. Additionally, it elucidates their noteworthy biomedical applications as well as their potential mechanisms of action, alongside summarizing their toxicity profiles. This article also highlights challenges and future prospects for further exploring MgO nanoparticles in the biomedical field. Existing literature indicates that synthesized magnesium oxide nanoparticles demonstrate substantial biocompatibility and display significant antibacterial, antifungal, anticancer, and antioxidant properties. Consequently, this review intends to enhance readers’ comprehension regarding recent advancements in synthesizing MgO nanoparticles through diverse approaches and their promising applications in biomedicine.

Effect of magnesium oxide nanoparticles and LED irradiation on the viability and differentiation of human stem cells of the apical papilla.

Currently, regenerative endodontic treatments are gaining more and more attention, and stem cells play a significant role in these treatments. In order to enhance stem cell proliferation and differentiation, a variety of methods and materials have been used. The purpose of this study was to determine the effects of magnesium oxide nanoparticles and LED irradiation on the survival and differentiation of human stem cells from apical papilla. The MTT test was used to measure the cell survival of SCAPs that had been exposed to different concentrations of magnesium oxide nanoparticles after 24 and 48h, and the concentration with the highest cell survival rate was picked for further studies. The cells were classified into four distinct groups based on their treatment: (1) control, which received no exposure, (2) exposure to magnesium oxide nanoparticles, (3) exposure to light emitting diode (LED) irradiation (635nm, 200mW/cm2) for 30s, (4) exposure simultaneously with magnesium oxide nanoparticles and LED irradiation. A green approach was employed to synthesize magnesium oxide nanoparticles. Quantitative real time PCR was used to measure the gene expression of osteo/odontogenic markers such as BSP, DSPP, ALP and DMP1 in all four groups after treatment, and Alizarin red S staining (ARS) was used to determine the osteogenic differentiation of SCAPs by demonstrating the Matrix mineralization. The highest viability of SCAPs was observed after 24h in concentration 1 and 10µg/mL and after 48h in concentration 1µg/mL, which were not significantly different from the control group. In both times, the survival of SCAPs decreased with increasing concentration of magnesium oxide nanoparticles (MgONPs). According to the results of Real-time PCR, after 24 and 48h, the highest differentiation of BSP, DMP1, ALP and DSPP genes was observed in the LED + MgONPs group, followed by MgONPs and then LED, and in all 3 experimental groups, it was significantly higher than control group (P < 0.05). Also, after 24 and 48h, the density of ARS increased in all groups compared to the control group, and the highest density was observed in the MgONPs + LED and MgONPs groups. This research concluded that exposure to SCAPs, MgONPs, and LED irradiation has a significant effect on enhancing gene expression of odontogenic/osteogenic markers and increasing matrix mineralization.

Synthesis of Periclase Phase (MgO) from Colloidal Cassava Starch Suspension, Dual Application: Cr(III) Removal and Pigment Reuse

Synthesis of Periclase Phase (MgO) from Colloidal Cassava Starch Suspension, Dual Application: Cr(III) Removal and Pigment Reuse

In vitro α-amylase/α-glucosidase, cytotoxicity and radical scavenging potential of Hypoxishemerocallidea synthesized magnesium oxide nanoparticles

The prevalence of diabetes has continued to increase over the past decade. Medicinal extract-synthesized nanoformulations incorporating trace elements found in the body have emerged as alternative therapeutic technology for the treatment of diabetes. In this study, magnesium oxide (MgO) nanoparticles were prepared using Hypoxishemerocallidea (HH) and investigated for their cytotoxicity, antioxidant, and antidiabetic potentials. MgO NPs were characterized by transmission electron microscopy (TEM), Fourier-transform infrared (FTIR) spectroscopy, and zeta-potential techniques. TEM analysis confirmed the 2D nanosheet-like morphology of the nanoparticles with particle size (diameter; 300 nm and length of about 1 µm) while the FTIR spectra showed functional groups correlating to the bioactive compounds of HH in the MgO NPs. Moderate antioxidant activity of MgO NPs was observed against DPPH (IC50 = 57.35 ± 0.28 µg/mL) and ABTS (IC50 = 52.08 ± 0.24 µg/mL). The nanoparticles were shown to be less toxic to normal embryonic (HEK293) and human liver (HEPG2) cell lines, with IC50 of 48.63 ± 0.73 and 32.39 ± 0.95 µg/mL, respectively compared to a known cytotoxic drug, doxorubicin which exhibited IC50 of 2.70 ± 0.32 and 8.62 ± 0.57 µg/mL respectively. Besides, the anti-hyperglycemic potential of the MgO NPs as demonstrated by α-amylase and α-glucosidase activities were significantly high with IC50 values of 33.03 ± 1.43 and 52.38 ± 3.06 µg/mL, respectively. These results were compared with an FDA-approved antidiabetic reference standard Acarbose, which exhibited IC50 values of 24.54 ± 1.55 and 6.54 ± 0.27 µg/mL. The results indicated that the HH bioinspired MgO NPs are capable of inhibiting meditators of diabetes and oxidative stress. This study further suggests that the MgO NPs synthesized using HH could be a good candidate for the management of diabetes and other inflammatory diseases due to their strong enzyme inhibition, efficient antioxidant properties, and biocompatibility.

Application of green synthesized magnesium oxide nanoparticles to prolong commercial availability of Vitis vinifera L

The objective of the study was to extend shelf life of Vitis vinifera (L.) by the application of green synthesized Magnesium oxide nanoparticles. Aqueous leaf extract of Azadirachta indica A. juss. and various concentrations of 20 mM, 30 mM, and 40 mM solutions of Magnesium nitrate hexa hydrate salt, were used to synthesize nanoparticles of different size. The characterization of nanoparticles was done by SEM, XRD, and UV. The antimicrobial activity of MgO NPs was evaluated for Azospirilum brasilense and Trichoderma viride, representative of microbes responsible for V. vinifera fruits spoilage. Nanoparticles with crystal size of 28.60 nm has more pronounced effect against microbes. The Shelf life of the Vitis vinifera L. was evaluated by application of 28.60 nm MgO NPs through T1 (nanoparticles coated on packaging), T2 (nanoparticles coated directly on fruit) at 4 °C and 25 °C. T1 at 4 °C was effective to extend the shelf life of Vitis vinifera (L) for an average of 20 days.

Synthesizing Magnesium Oxide through a 3D Experience

This lesson is from the Types of Reactions unit in a High School Chemistry class and focuses on the synthesis of magnesium oxide. The purpose of this lesson is for students to conduct a lab experiment, and understand, at an atomic level, how the reaction occurs. In collaboration between a Chemistry teacher and Educational Technology faculty member, one three-dimensional (3D) experience was created to support the visualization of the reaction and minimize the students’ misconceptions.

Synthesis of magnesium oxide and zinc oxide powders in a glow discharge plasma at atmospheric pressure

Investigation of the processes involved in the synthesis of magnesium oxide and zinc oxide powders using the thermal effects of an atmospheric-pressure glow discharge plasma in an inert gas flow are described. The discharge operates in a repetitively pulsed mode with pulse repetition rate of several tens of kilohertz and pulse duration up to 12 μs, discharge current of 600 mA and voltage up to 300 V. These parameters lead to thermal erosion of magnesium or zinc inserts in a molybdenum crucible. The chemical and phase composition of the erosion products were determined using TEM/EDS and X-ray diffraction analysis, and the composition of the plasma was assessed by optical emission spectrometry. This experimental approach allows fabrication of powders of these metal oxides with characteristic particle size 10–50 nm, and the formation of coatings of these materials in a one-step process.

Evaluation of biogenically synthesized MgO NPs anticancer activity against breast cancer cells

BackgroundMagnesium is recognized to have pharmacological potential, and its nanoformulation is anticipated to offer significant therapeutic effects, particularly against cancer. In this study, we analyzed the anticancer effect of biogenically synthesized magnesium oxide nanoparticles (MgO NPs) against breast cancer cells (MDA-MB-231). MethodsDifferent biological evaluations, such as cytotoxicity, cellular morphology, induction of apoptosis, generation of ROS, cell adhesion and cellular migration were estimated using well established methodology. ResultsThe biogenic MgO NPs exhibited increased cytotoxicity, induced apoptosis, enhanced formation of ROS, promoted cell adhesion and inhibited cellular migration in a dose-dependent manner, showing its therapeutic potential against MDA-MB-231 cells. ConclusionThe current study observed strong anticancer activity of MgO NPs against studied cancer cell lines. However, our study must be validated in an appropriate animal/xenograft model to authenticate the effectiveness of MgO NPs against breast cancer.

Influence of cobalt doping on chemical and green synthesized magnesium oxide nanoparticles for enhanced photocatalytic evaluation, adsorption studies, antimicrobial analysis and corrosion inhibition study

In this study, Cobalt-doped magnesium oxide nanoparticles (Co-MgO-Nps) were successfully synthesized using two different methods: co-precipitation via chemical synthesis and co-precipitation via a green synthesis approach. The originality of this research lies in the diverse applications that were explored using these nanoparticles. The study investigated various potential uses of Co-MgO-Nps, thereby highlighting their versatility and broad range of applications. X-ray diffraction (XRD), Fourier Transformed Infrared Spectroscopy (FTIR), Scanning Electron Microscope (SEM), Thermogravimetric Analysis (TGA), Energy-Dispersive X-ray (EDX) analysis, UV–Vis Diffusion Reflectance Spectroscopy, BET adsorption isotherm, Transmission Electron Microscope (TEM), Photoluminescence (PL), Zeta potential and particle size determination were used to investigate and characterize the synthesizedCo-MgO-Nps. The photocatalytical degradationability of Co-MgO-Npsunder UV light irradiation wasstudied on potentially dangerous malachite green dye. The synthesizedCo-MgO-Nps showed outstanding photocatalytic performance in various ppm concentrations. Adsorption studies using malachite green dye at numerous ppm levels revealed that the Co-MgO-Nps synthesized by green approach degraded the dye more quickly than those synthesized chemically. Antimicrobial activities were evaluated against E. coli with different concentrations; the findings confirmed that the Co-MgO-Nps produced from green synthesis approach showed superior antimicrobial activity than the chemically synthesized Co-MgO-Nps. The synthesized Co-MgO-Nps showed good efficiency for inhibiting corrosion of maraging steel by hydrochloric acid.

Green Synthesized Magnesium Oxide Nanoparticles Reinforce Osteogenesis Properties of Bacterial Cellulose Scaffolds for Bone Tissue Engineering Applications: An In Vitro Assessment.

The use of biocompatible scaffolds with appropriate characteristics to treat large bone defects has attracted significant attention. The main objective of the current study is to fabricate a 3D nanocomposite structure that contains green synthesized magnesium oxide nanoparticles (MgONPs) and bacterial cellulose (BC) nanofibres, as a bioscaffold for bone regeneration. In this experimental study, Camellia sinensis extract was used as the green method to synthesize MgONPs. The synthesized hydrogels were evaluated for their porosity, morphology, degradation rate, mechanical features, cell attachment, and cytocompatibility. Osteogenic differentiation was assessed by alkaline phosphatase (ALP) activity, real-time reverse transcription-polymerase chain reaction (RT-PCR), and alizarin red staining. MgONPs significantly increased both mechanical strength (P=0.009) and porosity (P=0.01) of the BC hydrogels. Human MG-63 osteoblast proliferation significantly increased in the MgONP-BC group compared to the pure BC group (P=0.003). Expression rates of both the ALP (P=0.001) and osteocalcin (OCN) genes were significantly enhanced in cells seeded on the MgONP-incorporated BC. MG-63 cells had significantly greater calcium deposition and ALP activity (P=0.002) on the MgONP-BC scaffold compared to the BC at day 21. The MgONP-BC scaffold can promote the osteogenic activity of osteoblast-like cells, which indicates its therapeutic potential for bone tissue regeneration.

Characterization and antimicrobial, antioxidant, and anti-proliferative activities of green synthesized magnesium oxide nanoparticles with shoot extracts of Plicosepalus curviflorus

Objective: To synthesize magnesium oxide nanoparticles using ethanol extract of shoots of Plicosepalus curviflorus (PC-MgONPs) and evaluate the antimicrobial, antioxidant, and anti-proliferative activities of PC-MgONPs. Methods: The green synthesized PC-MgONPs were characterized by ultraviolet-visible (UV), Fourier-transform infrared spectroscopy, zeta potential, energy dispersive X-ray, and scanning electron microscopy. Furthermore, we investigated total antioxidant capacity and antimicrobial and anti-proliferative activities using breast cancer cell lines (MDA-231). Results: The UV spectrum of PC-MgONPs showed a sharp absorption peak at 300 nm. The presence of magnesium, oxygen, and sodium was confirmed by energy dispersive X-ray analysis. Scanning electron microscopy revealed PC-MgONPs as roughly spherical granular structures with sizes ranging from 20.0 to 76.4 nm. PC-MgONPs showed considerable antimicrobial activities against Escherichia coli, Staphylococcus aureus, methicillin-resistant Staphylococcus aureus, Pseudomonas aeruginosa and Candida albicans with zones of inhibition of 11-17 mm. In addition, total antioxidant capacity and anti-proliferative activity of PC-MgONPs against MDA-231 cells were dose-dependent. Conclusions: The synthesized PC-MgONPs could be a potent antimicrobial, antioxidant and anti-cancer agent, which needs further investigation.

Synthesis of magnesium oxide nanoparticles and its application for photocatalytic removal of furfural from aqueous media: Optimization using response surface methodology

Furfural is a toxic compound with an LD50 of 65 mg/kg that is produced in many industries, such as paper industries, petrochemical industries, and oil refineries. This compound exists in various concentrations in these industries and is released into the environment. Therefore, removing this contaminant from the wastewater of production units is among the environmental priorities of every company. This study investigated the optimization of photocatalytic removal of furfural using synthesized magnesium oxide nanoparticles (MgONPs) by the response surface method (RSM). In this study, MgONPs were prepared by the direct precipitation method, and the characteristics of the nanoparticles (NPs) were examined using XRD and SEM analyses. The effect of various operating parameters, including pH (3, 7, and 11), contact time (15, 90, and 165 min), initial concentration of furfural (25, 150, and 275 mg/L), dose of NPs (125, 687.5, and 1250 mg/L), and UV radiation intensity (8, 15, and 30 µW/cm2) on furfural removal was explored. Furfural concentration was measured using a spectrophotometer at a wavelength of 277 nm. The number of samples was determined by the CCD method. Data were analyzed using MINITAB 16 software. The optimal removal conditions based on variance analysis and appropriate model were obtained at a pH of 6, NP dose of 1 g/L, initial concentration of 250 mg/L, reaction time of 160 min, and UV radiation intensity of 30 µW/m2. Under these conditions, the removal efficiencies for both furfural and COD were 99.97%. The results of the analysis of the synthesized MgONPs also showed that the direct precipitation method is a fast and low-cost method for the synthesis of MgONPs. Results showed that the photocatalytic process in the presence of synthesized MgONPs is highly efficient in removing furfural from aqueous media.

Exploring the nano-fungicidal efficacy of green synthesized magnesium oxide nanoparticles (MgO NPs) on the development, physiology, and infection of carrot (Daucus carota L.) with Alternaria leaf blight (ALB): Molecular docking

In this research, green synthesized magnesium oxide nanoparticles (MgO NPs) from lemon fruit extracts and their fungicidal potential was evaluated against Alternaria dauci infection on carrot (Daucus carota L.) under greenhouse conditions. The scanning and transmission electron microscopy (SEM and TEM) and ultra-violet (UV) visible spectroscopy were used to validate and characterize MgO NPs. The crystalline nature of MgONPs was determined using selected area electron diffraction (SAED). MgO NPs triggered substantial antifungal activity against A. dauci when exposed to 50 and 100 mg L−1 concentrations but the higher antifungal potential was noticed in 100 mg L−1 under in-vitro conditions. In fungal inoculated plants, a marked decrease in growth, photosynthetic pigments, and an increase in phenol, proline contents, and defense-related enzymes of carrot were seen over control (distilled water). However, foliar application of MgO NPs at 50 and 100 mg L−1 resulted in significant improvement of plant growth, photosynthetic pigments, phenol and proline contents, and defense enzymes activity of carrots with and without A. dauci infection. Spraying of MgO NPs at 100 mg L−1 had more plant length (17.11%), shoot dry weight (34.38%), plant fresh weight (20.46%), and root dry weight (49.09%) in carrots when challenged with A. dauci over inoculated control. The leaf blight indices and percent disease severity were also reduced in A. dauci inoculated plants when sprayed with MgO NPs. The non-bonding interactions of Alternaria genus protein with nanoparticles were studied using molecular docking.