Synthesis Of Oligonucleotide Analogues Research Articles

Studies on Nucleoside H-Phosphonothioates as Synthons in the Synthesis of Oligonucleotide Analogues

Recently, we have reported that H-phosphonothioate diesters1 are versatile synthetic intermediates for the preparation of phosphorothioates, phosphorodithioates, or other phosphodiester analogues. The most suitable coupling agents to produce Hphosphonothioate diesters were found to be various chlorophosphates1 [e.g., 2-chloro-5,5-dimethyl-2-oxo-2λ5-1,3,2-dioxaphosphinane (NEP), diphenyl phosphorochloridate]. These reagents secure the required chemoselectivity during the condensation and are unreactive towards the P-H function in H-phosphonothioate diesters1,2.

Synthesis of oligonucleotide analogues by using phosphoramidite reagents derived from 2,2-bis(hydroxymethyl)malonates

Synthesis of oligonucleotide analogues by using phosphoramidite reagents derived from 2,2-bis(hydroxymethyl)malonates

Synthesis of Oligonucleotide Analogues with Modified Backbones

Synthesis of Oligonucleotide Analogues with Modified Backbones

Nucleoside H-phosphonates. X. Studies on nucleoside hydrogenphosphonothioate diester synthesis

Synthesis and chemical properties of nucleoside H-phosphonothioates are discussed in the context of possible application of these compounds as intermediates in the synthesis of oligonucleotide analogues.