Synthesis Of Fluorescent Dyes Research Articles

New High-Performance Fluorescent Dye Scaffolds: Applications for Bioimaging and Biosensing

AbstractFluorescence imaging in the shortwave infrared region (SWIR, 850–2500 nm) window has become an indispensable tool in biomedical research because it has weaker absorption, less light scattering, and less background fluorescence in this window. As we know, the design and synthesis of fluorescent dyes and fluorescent probes are the cores of fluorescence imaging and biosensing. Thus, their photophysical mechanisms exploring and bioanalytical applications are multidisciplinary and cutting-edge research topics. In this regard, we have been working on high-performance fluorescent dyes and fluorescent probes for years. In short, we have developed a series of bright, stable, aggregation-resistant, SWIR fluorescent dyes ECXs, based on a carbon-bridged spiro ring strategy. We also developed a series of high-performance SWIR fluorescent dyes CXs and Chrodols, which combine the structural advantages of cyanine and xanthenoid dyes. Based on these new SWIR scaffolds, we further constructed some activatable SWIR fluorescent probes with OFF-ON or ratiometric properties for biosensing in vivo. Therefore, the main line of our work is to gain an in-depth understanding of the photophysical mechanisms of fluorescent dyes, to create high-performance luminescent dyes, and to further develop fluorescent probes for bioimaging and biosensing.1 Introduction2 ECX Dyes Based on a Carbon-Bridged Spiro Ring Strategy3 Fluorescent Dyes and Fluorescent probes Combining the Structures of Cyanine and Xanthenoid Dyes3.1 CX Series Dyes3.2 A Functionalized Modified CX probe NRh3.3 CX-like Dyes with a Secondary Amino Py-23.4 Chrodol Series Dyes4 Conclusion and Future Prospects

A green method for the synthesis of coumarin dye and its application to hypochlorite recognition

In modern organic synthesis, green organic synthesis technology has become an important research direction in the field of chemistry and chemical engineering. Coumarin dyes play an important role in industrial chemistry, biochemistry and material science, so the synthesis of fluorescent dyes has been an important problem in modern organic synthesis. Therefore, the design and discovery of green organic synthesis of coumarin derivatives is very important. Hypochlorous acid/hypochlorite as a ROS plays an important role in diagnosis and treatment of diseases. To date, many probes have been developed for HClO/ClO− detection. Among them, some probes have poor selectivity and slow response time for HClO/ClO−, which greatly limits the practical application value of probes. In this work, the reaction of 7-(diethylamino)-2H-chromene-2-thione with hypochlorite produces 7-(diethylamino)-2H-chromen-2-one, which is a novel green synthesis method of coumarin dye. The experimental results showed that the reaction is a ratiometric, selective and sensitive fluorescence system for the recognition of hypochlorite. This method has been successfully to detection of hypochlorite in HeLa/HNEpC cells, zebrafish and actual sample detection.

Analysis of 3-d molecular distribution with the digital imaging microscope

Advances in digital imaging microscopy and in the synthesis of fluorescent dyes allow the determination of 3D distribution of specific proteins, ions, GNA or DNA in single living cells. Effective use of this technology requires a combination of optical and computer hardware and software for image restoration, feature extraction and computer graphics.The digital imaging microscope consists of a conventional epifluorescence microscope with computer controlled focus, excitation and emission wavelength and duration of excitation. Images are recorded with a cooled (-80°C) CCD. 3D images are obtained as a series of optical sections at .25 - .5 μm intervals.A conventional microscope has substantial blurring along its optical axis. Out of focus contributions to a single optical section cause low contrast and flare; details are poorly resolved along the optical axis. We have developed new computer algorithms for reversing these distortions. These image restoration techniques and scanning confocal microscopes yield significantly better images; the results from the two are comparable.

ChemInform Abstract: Synthesis of Fluorescent Dyes. Part 15. Synthesis of New Reagents for the Fluorescence Derivatisation of Thiols and Alcohols.

AbstractAusgehend vom Benzothiazol (I) werden die selektiven Thiolderivatisierungsreagenzien (IV) und (Vb) synthetisiert.